Molecular Characterization of Escherichia Coli Isolates from Food Animals

The present study was carried out for isolation and molecular characterization of Escherichia coli from faecal and meat samples of food animals viz. cattle, pigs and poultry. A total of 66 E. coli isolates were recovered from 420 samples of different food animals and further confirmed by PCR targeting E. coli specific uidA gene. These isolates were sensitive to chloramphenicol and ciprofloxacin and resistant to ampicillin and cloxacillin. Out of 66 isolates, 42 were typed into 13 different ‘O’ serogroups, 13 untypable and remaining 11 were identified as rough. Serogroup O84, O101, O118, O120 and O147 were predominant and serogroup O118 was found to be common in the samples of all 3 species of food animals. Five (7.57%) and 3 (4.54%) of E. coli isolates were found to harbor virulent genes, stx2 and est, respectively. Twenty representative E. coli isolates selected randomly from 20 different locations were subjected to molecular typing by PCR targeting Repetitive Extragenic Palindromic (REP) sequences. The region specific molecular types of E. coli could not be detected by using REP-PCR based discrimination.

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Occurrence and Characterization of Escherichia coli O157 and Other Serotypes in Raw Meat Products in Morocco

Journal of Food Protection ◽

10.4315/0362-028x-71.10.2082 ◽

2008 ◽

Vol 71 (10) ◽

pp. 2082-2086 ◽

Cited By ~ 5

Author(s):

LUCIANO BENEDUCE ◽

GIUSEPPE SPANO ◽

ARI Q. NABI ◽

FRANCESCO LAMACCHIA ◽

SALVATORE MASSA ◽

...

Keyword(s):

Escherichia Coli ◽

Susceptibility Testing ◽

Meat Products ◽

Molecular Techniques ◽

Escherichia Coli O157 ◽

Raw Meat ◽

E Coli ◽

Meat Samples ◽

The City

In this study, 100 raw meat samples were collected from 15 local Moroccan butcheries in five different areas of the city of Rabat during a period of 4 months. Overall, 7 of 15 butcheries from three areas of the city yielded strains of Escherichia coli O157. Single isolates from 9 (9%) of 100 raw meat samples were biochemically and serologically confirmed as E. coli O157. Using molecular techniques, two strains were positive for the Shiga toxin, with two additional strains containing an attaching-effacing gene. All potentially virulent serotypes isolated from these meat samples showed distinct pulsed-field gel electrophoresis profiles. Based on antibiotic susceptibility testing, more than 70% of the isolates were resistant to ampicillin and clavulanic acid–amoxicillin. Moreover, one strain was resistant to more than three antibiotics. Our study represents the first survey of E. coli O157 and related serotypes in raw meat products in Morocco.

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Escherichia coli O157:H7: distribution, molecular characterization, antimicrobial resistance patterns and source of contamination of sheep and goat carcasses at an export abattoir, Mojdo, Ethiopia

BMC Microbiology ◽

10.1186/s12866-019-1590-8 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 5

Author(s):

Solomon Abreham ◽

Akafete Teklu ◽

Eric Cox ◽

Tesfaye Sisay Tessema

Keyword(s):

Escherichia Coli ◽

Molecular Characterization ◽

Virulence Genes ◽

Latex Agglutination ◽

Human Infection ◽

Systematic Random Sampling ◽

E Coli ◽

Source Of Infection ◽

Resistance Patterns

Abstract Background Cattle have been identified as a major reservoir of E. coli O157:H7 for human infection; the ecology of the organism in sheep and goats is less understood. This study was carried out to determine prevalence, source of infection, antibiotic resistance and molecular characterization of Escherichia coli O157: H7 isolated from sheep and goat. Methods Systematic random sampling was carried out at Modjo export abattoir, Ethiopia, from November 2012 to April 2013 to collect 408 samples from 72 sheep and 32 goats. Samples collected were skin swabs, fecal samples, intestinal mucosal swabs and the inside and outside part of carcasses as well as carcass in contacts such as workers hands, knife, hook and carcass washing water. Then, samples were processed following standard bacteriological procedures. Non-Sorbitol fermenting colonies were tested on latex agglutination test and the positives are subjected to PCR for detection of attaching and effacing genes (eaeA) and shiga toxin producing genes (stx1 and stx2). All E. coli O157:H7 isolates were checked for their susceptibility pattern towards 15 selected antibiotics. Results E. coli O157:H7 were detected in only 20/408 samples (4.9%). Among these 20 positive samples, 70% (14/20), 25% (5/20) and 5% (1/20) were from sheep, goats and knife samples, respectively. No significant associations were found between carcasses and the assumed sources of contaminations. Of all the 20 isolates virulence genes were found in 10 (50%) of them; 3 (15%) with only the eaeA gene and 7(35%) expressing eaeA and stx2 genes. All the isolates were susceptible to Norfloxacin (NOR) (100%). Conclusions The presence of virulence genes shows E. coli O157:H7 is a potential source of human infection in Ethiopia.

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Genetic Characterization of IncI2 Plasmids CarryingblaCTX-M-55Spreading in both Pets and Food Animals in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02155-12 ◽

2013 ◽

Vol 57 (6) ◽

pp. 2824-2827 ◽

Cited By ~ 42

Author(s):

Luchao Lv ◽

Sally R. Partridge ◽

Liangying He ◽

Zhenling Zeng ◽

Dandan He ◽

...

Keyword(s):

Escherichia Coli ◽

Genetic Characterization ◽

Content Type ◽

E Coli ◽

Food Animals ◽

Plasmid Backbone

ABSTRACTpHN1122-1 carryingblaCTX-M-55, from anEscherichia coliisolate from a dog, was completely sequenced. pHN1122-1 has an IncI2 replicon and typical IncI2-associated genetic modules, includingmok/hok-finO-yafA/B,nikABC, and two transfer regions,traandpil, as well as a shufflon.blaCTX-M-55is found within a 3.084-kb ISEcp1transposition unit that includes a fragment of IncA/C plasmid backbone. pHN1122-1 and closely related plasmids were identified in otherE. coliisolates from animals in China.

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Molecular Characterization of UpaB and UpaC, Two New Autotransporter Proteins of Uropathogenic Escherichia coli CFT073

Infection and Immunity ◽

10.1128/iai.05322-11 ◽

2011 ◽

Vol 80 (1) ◽

pp. 321-332 ◽

Cited By ~ 55

Author(s):

Luke P. Allsopp ◽

Christophe Beloin ◽

Glen C. Ulett ◽

Jaione Valle ◽

Makrina Totsika ◽

...

Keyword(s):

Escherichia Coli ◽

Molecular Characterization ◽

Host Responses ◽

Upec Strain ◽

Content Type ◽

Pcr Screening ◽

E Coli ◽

K 12 ◽

Encoding Genes

ABSTRACTUropathogenicEscherichia coli(UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter (AT) subgroup of proteins. The genome-sequenced prototype UPEC strain CFT073 contains 11 putative AT-encoding genes. In this study, we have performed a detailed molecular characterization of two closely related AT adhesins from CFT073: UpaB (c0426) and UpaC (c0478). PCR screening revealed that theupaBandupaCAT-encoding genes are common inE. coli. TheupaBandupaCgenes were cloned and characterized in a recombinantE. coliK-12 strain background. This revealed that they encode proteins located at the cell surface but possess different functional properties: UpaB mediates adherence to several ECM proteins, while UpaC expression is associated with increased biofilm formation. In CFT073,upaBis expressed whileupaCis transcriptionally repressed by the global regulator H-NS. In competitive colonization experiments employing the mouse UTI model, CFT073 significantly outcompeted itsupaB(but notupaC) isogenic mutant strain in the bladder. This attenuated phenotype was also observed in single-challenge experiments, where deletion of theupaBgene in CFT073 significantly reduced early colonization of the bladder.

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Presence and Characterization of a Mosaic Genomic Island Which Distinguishes Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H− from E. coli O157:H7

Applied and Environmental Microbiology ◽

10.1128/aem.71.8.4875-4878.2005 ◽

2005 ◽

Vol 71 (8) ◽

pp. 4875-4878 ◽

Cited By ~ 16

Author(s):

Andreas Janka ◽

Georg Becker ◽

Anne-Katharina Sonntag ◽

Martina Bielaszewska ◽

Ulrich Dobrindt ◽

...

Keyword(s):

Escherichia Coli ◽

Genomic Island ◽

Enterohemorrhagic Escherichia Coli ◽

Resistance Locus ◽

Escherichia Coli O157 ◽

Related Sequence ◽

E Coli ◽

Pcr Targeting

ABSTRACT A mosaic genomic island comprising Shigella resistance locus (SRL) sequences flanked by segments of Escherichia coli O157:H7 strain EDL933 O islands 43, 81, and 82 was identified in sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H− strain 493/89. This mosaic island is absent from strain EDL933. PCR targeting the SRL-related sequence is a useful tool to distinguish SF EHEC O157:H− from EHEC O157:H7.

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Molecular Characterization of Escherichia coli Isolates Carrying mcr-1, fosA3, and Extended-Spectrum-β-Lactamase Genes from Food Samples in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00064-17 ◽

2017 ◽

Vol 61 (6) ◽

Cited By ~ 10

Author(s):

Xiaobo Liu ◽

Ruichao Li ◽

Zhiwei Zheng ◽

Kaichao Chen ◽

Miaomiao Xie ◽

...

Keyword(s):

Public Health ◽

Escherichia Coli ◽

Molecular Characterization ◽

Food Products ◽

Multidrug Resistant ◽

Food Samples ◽

Content Type ◽

E Coli ◽

Extended Spectrum

ABSTRACT This study surveyed the prevalence of mcr-1 in extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains of food origin in China and identified strains that carried mcr-1, fosA3, and ESBL genes, which were carried in various plasmids. The mcr-1 and ESBL genes could be cotransferred by one or more types of plasmids. The presence of these multidrug-resistant E. coli strains in food products might pose a huge threat to public health.

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Molecular Characterization of β-Lactamase Producing Genes and Integrons in Diarrheagenic Escherichia Coli From Diarrheal Children Less Than Five Years of Age in Ouagadougou, Burkina Faso.

10.21203/rs.3.rs-628039/v1 ◽

2021 ◽

Author(s):

Rene Dembele ◽

Wendpoulomdé A.D. Kaboré ◽

Issiaka Soulama ◽

Oumar Traoré ◽

Nafissatou Ouédraogo ◽

...

Keyword(s):

Escherichia Coli ◽

Molecular Characterization ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Control Measures ◽

Diffusion Method ◽

Biochemical Tests ◽

Disk Diffusion Method ◽

E Coli

Abstract Background The aim of this study was to determine the resistance of diarrheagenic Escherichia coli strains to β-lactams antibiotics and to perform the molecular characterization of Extended Spectrum β-lactamases (ESBL) and integrons genes. Methods This study was carried out from August 2013 to October 2015 and involved 31 DEC strains isolated from diarrheal stools samples collected from children less than five years of age. The identification and characterization of DEC strains was done through the standard biochemical tests those were confirmed using API 20E and Polymerase Chain Reaction (PCR). The determination of antimicrobial resistance was realized by the disk diffusion method then an amplification of the β-lactamase resistance genes and integrons by PCR was done. Results Out of the 419 E. coli strains identified, 31 isolates (7.4%) harbored the DEC virulence genes. From these DEC, 21 (67.7%) were ESBL-producing E. coli. Susceptibility to ESBL-producing E. coli showed that the majority of isolates were highly resistant to amoxicillin (77.4%), amoxicillin clavulanic acid (77.4%) and piperacillin (64.5%). The following antibiotic resistance genes and integron were identified from the 31 DEC isolates: blaTEM (6.5%), blaSHV (19.4%), blaOXA (38.7%) blaCTX−M (9.7%), Int1 (58.1%) and Int3 (19.4%). No class 2 integrons (Int2) was characterized. Conclusions Because of the high prevalence of multidrug-resistant ESBL organisms found in this study among pediatric patients, there is a need of stringent pediatric infection control measures.

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Genetic Characterization of Plasmid-mediated qepA Gene Among ESBL-producing Escherichia Coli Isolates in Mexico

10.21203/rs.3.rs-547553/v1 ◽

2021 ◽

Author(s):

Humberto Barrios-Camacho ◽

Josefina Duran-Bedolla ◽

Jesus Silva-Sanchez ◽

Luis Lozano-Aguirre ◽

Fernando Reyna-Flores ◽

...

Keyword(s):

Escherichia Coli ◽

Molecular Characterization ◽

Genetic Characterization ◽

Insertion Sequences ◽

Plasmid Sequence ◽

E Coli ◽

Multiple Copies ◽

Chromosomal Mutations ◽

Sequence Types

Abstract A molecular characterization of a plasmid-born qepA gene in (ESBL)-producing E. coli clinical isolates were performed. An 2.63% (11/418) were qepA positive isolates, of which a 90.0% carried CTX-M-15 (9/11) and SHV-12 (1/11). All isolates showed chromosomal mutations in the gyrA and parC genes. The clonal groups A, B and C were identified and belonged to, respectively, phylogroups A, B1 and D, as well as the sequence types 205, 405 and 617. Several plasmid profiles were determined with incompatibility groups FIA, FIB and FII. The genetic environment of the qepA in plasmid pEC8020 was different from those reported previously. The plasmid sequence included genes conferring resistance to β-lactams (blaCTX-M-15), macrolides (mphA), fluoroquinolones (qepA1), trimethoprim (dfrB4) and sulphonamides (sul1). Likewise, the IncF-pEC8020 plasmid carried several insertion sequences including ISCR3, IS6100 and multiple copies of IS26. This work contributes to the epidemiology and genetics of plasmid-born qepA genes of ESBL-producing E. coli.

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Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

Indian Journal of Animal Research ◽

10.18805/ijar.b-3972 ◽

2020 ◽

Author(s):

T. Ramasamy ◽

S. Keerthana ◽

M.R. Srinivasan ◽

D. Chandrasekar ◽

K. Porteen ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Molecular Characterization ◽

Antibiotic Sensitivity ◽

Growth Media ◽

E Coli ◽

Sensitivity Pattern

Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

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Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China

Journal of Medical Microbiology ◽

10.1099/jmm.0.012229-0 ◽

2009 ◽

Vol 58 (11) ◽

pp. 1449-1453 ◽

Cited By ~ 51

Author(s):

Li Yuan ◽

Jian-Hua Liu ◽

Gong-Zheng Hu ◽

Yu-Shan Pan ◽

Zhi-Ming Liu ◽

...

Keyword(s):

Escherichia Coli ◽

Molecular Characterization ◽

Antimicrobial Agents ◽

Animal Health ◽

Henan Province ◽

E Coli ◽

Nucleotide Base ◽

Extended Spectrum ◽

M 14

Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has spread rapidly worldwide and poses a serious threat to human and animal health. This study collected 51 non-replicate E. coli isolates from 14 different chicken farms in Henan Province in China from December 2007 to August 2008. The prevalence of ESBL-producing E. coli, molecular characterization of the ESBL-related bla genes, including bla TEM, bla SHV and bla CTX-M, and the susceptibilities of these bacteria to various antimicrobial agents were determined. Thirty-one of the 51 isolates were positive for an ESBL phenotype and 29 of these isolates carried one or more bla genes. Twenty-two isolates harboured bla TEM genes and 15 isolates carried bla CTX-M genes (one CTX-M-14, three CTX-M-24 and 11 CTX-M-65). One isolate carried bla TEM -57; the remaining bla TEM isolates carried bla TEM-1 with one silent nucleotide base variation (T18C). We believe that this is the first study to report TEM-57 in E. coli isolates. All isolates harbouring bla CTX-M-24 and bla CTX-M-14 and five of the bla CTX-M-65 isolates also harboured the bla TEM-1 gene. To our knowledge, this study is the first to describe detection of CTX-M-65-producing E. coli isolated from chickens. None of the isolates contained the bla SHV gene. Conjugation experiments demonstrated that bla CTX-M and bla TEM genes could be transferred to E. coli DH5α. The results indicate that ESBL frequency has reached an alarming level in chicken isolates in China, with TEM-1 and CTX-M-65 enzymes being the two predominant β-lactamases detected.

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