scholarly journals Acetylcysteine in random skin flap in rats

2005 ◽  
Vol 20 (2) ◽  
pp. 121-123 ◽  
Author(s):  
Luiz Eduardo Felipe Abla ◽  
Heitor Carvalho Gomes ◽  
Sandro Percario ◽  
Lydia Masako Ferreira
Keyword(s):  
Normal Skin ◽  
Skin Flap ◽  
Control Group ◽  
Distilled Water ◽  
Significant Difference ◽  
Group Control ◽  
The Mean ◽  
Flap Elevation ◽  
Skin Samples

PURPOSE: Analyze the ability of Acetylcysteine to reduce distal necrosis in a random skin flap, in the rat. METHODS: The present study utilized 28 adult male Wistar-EPM rats distributed, at random, in two groups of 14 animals. Control group rats (CG) received distilled water and Acetylcysteine group animals (NACG) received NAC (300 mg/kg) by oral infusion, 15 minutes before flap elevation. On the seventh postoperative day, percentage of distal necrosis was determined and skin samples collected in order to allow determination of MDA levels. RESULTS: The mean necrotic area in CG group (control) was 66 % and in NACG group (Acetylcysteine) 52 %, a statistically significant difference according to the Mann-Whitney test (U calc = 25; U crit = 45). MDA levels were lower in the CG flap skin samples than in the NACG samples (U calc = 24; U crit = 45), the oposite being true in the normal skin samples (U calc = 10; U crit = 45). CONCLUSION: Acetylcysteine was effective, according to the model used, reducing the percentage of distal necrosis in NACG rats.

scholarly journals Terazosin and propranolol as blockers to the deleterious effect of nicotine in a random skin flap, in the rat

2004 ◽  
Vol 19 (3) ◽  
pp. 186-195 ◽  
Author(s):  
Andre V. Fonseca ◽  
Lydia Masako Ferreira ◽  
Heitor C. Gomes ◽  
Sandro Percário
Keyword(s):  
Postoperative Period ◽  
Deleterious Effect ◽  
Skin Flap ◽  
Mean Value ◽  
Control Group ◽  
Distilled Water ◽  
Propranolol Group ◽  
Flap Elevation ◽  
Skin Samples

PURPOSE: To evaluate the effect of Terazosin and Propranolol on the prevention of necrosis induced by nicotine, in a random skin flap. METHODS: This study utilized 32 adult male Wistar-EPM rats divided, at random, into four groups of eight animals each. All the 32 animals received nicotine (2 mg/kg/day) subcutaneously, for one week before and one week after flap elevation. CG (Control) group received distilled water (0.2 ml/day) by gavage and saline (0.5 ml) intraperitoneally, for seven days in the postoperative period. TG (Terazosin) group received terazosin hydrochloride (3 mg/day) by gavage and saline, intraperitoneally, for seven days in the postoperative period. PG (Propranolol) group received propranolol (1.5 mg/day) intraperitoneally and distilled water, by gavage, following the stablished pattern. TPG (Terazosin + Propranolol) group received both drugs. On the seventh postoperative day, the distal necrotic area of the flaps was determined via the paper template method. Blood and skin samples were collected in order to allow determination of Malondialdehyde (MDA) levels RESULTS: The control group had a mean value of 39.5 % of necrosis; the Terazosin group 25.1 %; the Propranolol group 34.5 % and the Terazosin + Propranolol group 26.2 % of necrosis. MDA levels in the serum and in the skin samples behave similarly, with an exception regarding Propranolol group in this case. CONCLUSION: Terazosin is effective in the prevention of necrosis in this animal model and Propranolol is not effective in this case.

scholarly journals Uric acid as a monitor of oxidative stress in a random skin flap in rats

2003 ◽  
Vol 18 (6) ◽  
pp. 502-508 ◽  
Author(s):  
Sueli Teresinha Freire ◽  
Lydia Masako Ferreira ◽  
Heitor Carvalho Gomes ◽  
Sandro Percario
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Adult Male ◽  
Normal Skin ◽  
Skin Flap ◽  
Time Schedule ◽  
Skin Samples

PURPOSE: To verify the possible role of uric acid as a monitor of oxidative stress in a random skin flap, in the rat. METHODS: This study utilized 21 adult male Wistar-EPM rats divided, at random, in three groups of seven animals, to evaluate the behavior of uric acid levels during oxidative stress zero, 5 and 15 minutes after elevation of the flap. According to the time schedule, skin samples were collected in order to allow determination of uric acid and malondialdehyde (MDA) levels. RESULTS: Uric acid levels in the skin samples did not change with time, but the values in normal skin were higher since the beginning. Malondialdehyde (MDA) levels in the skin samples remained stable throughout. CONCLUSION: Uric acid levels in the flap skin were lower than those in the normal skin, denoting local oxidative stress.

scholarly journals DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF MEGLUMINE IN BULK

2014 ◽  
Vol 10 (3) ◽  
pp. 2325-2328
Author(s):  
Barge V. U. ◽  
Jadhav P. B. ◽  
Attarde S. R. ◽  
Kodre K. V. ◽  
Patil R. Y
Keyword(s):  
Quantitative Determination ◽  
Distilled Water ◽  
Mean Values ◽  
Spectrophotometric Methods ◽  
Linearity Range ◽  
Significant Difference ◽  
The Mean ◽  
Development And Validation ◽  
Third Derivative

UV, first, second and third derivative spectrophotometric methods have been developed for the determination of meglumine. The solutions of standard and sample were prepared in distilled water. For the first method i.e. calibration curve UV spectrophotometric method, the quantitative determination of the drug was carried at 254 nm and the linearity range was found to be 10 – 60 µg/ml. For the first, second, third derivative spectrophotometric methods the drug was determined at 247 nm, 216 nm, 266 nm with the linearity range 10 – 60 µg /ml. The calibration graphs constructed at their wavelength of determination were found to be linear for UV and derivative spectrophotometric methods. All the proposed methods have been extensively validated. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations.

scholarly journals Anthropogenetic variability in the groups of homo- and heterosexually oriented individuals

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 765-776
Author(s):  
Suzana Cvjeticanin
Keyword(s):  
Genetic Variability ◽  
Population Genetic ◽  
Control Group ◽  
Population Genetic Study ◽  
Test Results ◽  
Mean Values ◽  
Significant Difference ◽  
The Mean ◽  
Distribution Type

This population-genetic study compares morphophysiological and genetic variability in a group of homosexually oriented individuals from Serbia (N=96) with control group of heterosexual individuals (N=96) using a test of determination of homozygously recessive characteristics in humans (HRC-test). Results of our study revealed a statistically significant difference in the mean values of genetic homozygosity (control group 5.0+0.2 ; homosexuals 3.4 +0.1 HRCs, out of 20 observed characteristics) the differences in the distribution type, as well as in the variances of presence of specific combinations of such traits. These results suggest a complex polygenic difference between two observed systems. There is no difference in the degree of genetic homozygosity between the genders in each tested group of individuals. However, both homosexually oriented females and males have significantly lower mean values of HRCs compared to female and male heterosexuals.

scholarly journals Anthropogenetic variability in groups of children from regular and special schools from different localities in Serbia

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 743-751 ◽  
Author(s):  
Slavko Brankovic ◽  
Suzana Cvjeticanin
Keyword(s):  
Control Group ◽  
Population Genetic Study ◽  
Control Groups ◽  
Special Schools ◽  
Mean Values ◽  
Significant Difference ◽  
Mental Abilities ◽  
The Mean ◽  
Physical Capacities

This population-genetic study compares morpholophysiological and genetic variability in five control groups of individuals (children from five regular schools, N= 996) with children from that many special schools (N= 736) from Serbia, by using a test of determination of homozygously recessive characteristics in humans (HRC-test). Genetic homozygosity degree showed not only statistically significant difference between the mean values obtained for two groups of studied samples (control group 6.95? 0.07; children from special schools 8.63? 0.08 HRCs, out of 30 analyzed characteristics), but also differences in the type of distribution, as well as the presence of specific combinations of such traits. Results of comparisons done in different places (Kraljevo, Nis, Vranje, Leskovac, Pirot) showed the same tendency- the increase of genetic homozygosity and relative decrease of variability in samples of children from special schools. The number of HRCs among individuals from control groups varied from 2 to 15/30, and from 3 to 16/30 among children from special schools. It is possible that increased recessive homozygosity present in the group of children from special schools leads to increase of genetic loads, what may cause easier expression of some physiological and mental abilities that children from special schools have.A great individual variation in amount of genetic homozygosity that exists among human individuals may influence their potentials for different kinds of adaptation, including their mental abilities, physical capacities or resistance to different diseases.

scholarly journals Evaluation of Surface Roughness of Composite, Compomer and Carbomer After Curing Through Mylar Strip and Glycerin: A Comparative Study

2019 ◽  
Vol 11 (1) ◽  
pp. 12-15
Author(s):  
Asli Topaloglu-Ak ◽  
Dilara Çayırgan ◽  
Melisa Uslu
Keyword(s):  
Surface Roughness ◽  
Silicone Rubber ◽  
Confidence Level ◽  
Control Group ◽  
Curing Conditions ◽  
Significant Difference ◽  
Group Control ◽  
The Mean ◽  
Oxygen Group ◽  
Roughness Measurements

Aim: To compare the effect of mylar strip and glycerin topical application on the surface roughness of composite, compomer, and carbomer during polymerization. Materials and Method: Each of 45 disc-shaped specimens of Z250, Dyract, Carbomer, were prepared according to manufacturer’s instructions and molded in silicone rubber molds by one operator (5 mm diameter × 2 mm thickness; n = 135). Each restorative group was divided into 3 groups as oxygen group (control), mylar strip, and glycerin group (n = 15). In the control group, specimens were light cured in the open air for 40 s, whereas in the test groups they were covered either with a mylar strip or a drop of glycerin solution and cured for 40 s. Glycerin was rinsed off before polishing. Sof-Lex discs were used for polishing before the surface roughness measurements by a profilometer. The obtained data were statistically analyzed using two-way analysis of variance at a confidence level of 95% ( P < .05). Results: The mean surface roughness values of composite, compomer, and carbomer polymerized in air, through mylar strip and glycerin showed no statistically significant difference ( P > .05). Conclusion: Mylar strip and glycerin application did not affect the surface roughness of the composite, compomer, and carbomer. Polishing may mask the effect of different curing conditions.

Detection of Circulating Tissue Factor and Factor VII in a Normal Population

1996 ◽  
Vol 75 (05) ◽  
pp. 772-777 ◽  
Author(s):  
Sybille Albrecht ◽  
Matthias Kotzsch ◽  
Gabriele Siegert ◽  
Thomas Luther ◽  
Heinz Großmann ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Normal Population ◽  
Mean Value ◽  
Factor Vii ◽  
Significant Difference ◽  
Age Dependent ◽  
The Mean ◽  
Highly Correlated ◽  
And Gender

SummaryThe plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation.

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

The Baby Shark (Songs Heard Affecting Resuscitation Kinetics) study

2020 ◽  
pp. bmjstel-2020-000657
Author(s):  
Rebecca Singer ◽  
Grace Leo ◽  
Tessa Davis ◽  
Ben Lawton ◽  
Henry Goldstein ◽  
...  
Keyword(s):  
Control Group ◽  
Control Groups ◽  
Target Zone ◽  
Compression Depth ◽  
Significant Difference ◽  
Overall Performance ◽  
The Mean ◽  
Training Exercises ◽  
And Control ◽  
Musical Cues

Previous research has examined the utilisation of musical cues to improve the performance of cardiopulmonary resuscitation (CPR) delivered in training environments. We postulated a musical cue that is both contemporary and transcends cultures may improve CPR performance. Our aim was to establish whether chest compressions are performed with improved rate and depth if a song of a fixed beat (PinkFong’s ‘Baby Shark’ with a tempo of 115 beats per minute (bpm) and 15 beats in each verse) is played to a healthcare professional immediately before undertaking CPR compared to whale noises (a non-metronomic rhythm). 58 Participants of a paediatric conference (majority doctors) were randomly assigned to listen to a minute of Baby Shark (28) or whale song (30) and then undertake a minute of CPR. There was no significant difference in the mean compression rate between the Baby Shark and control groups, with the groups achieving 121 and 125 bpm, respectively (p=0.18). In relation to compression depth within the target zone, the Baby Shark group had more compressions completed within the target zone (55%) than the control group (39%) although this difference was not significant (p=0.08). Listening to Baby Shark prior to undertaking simulated CPR does not improve overall performance, but there is a potential tendency to improve adequate compression depth which may be beneficial in training exercises.

scholarly journals Corneal Biomechanical Parameters and Central Corneal Thickness in Glaucoma Patients, Glaucoma Suspects, and a Healthy Population

2021 ◽  
Vol 10 (12) ◽  
pp. 2637
Author(s):  
Mª. Ángeles del Buey-Sayas ◽  
Elena Lanchares-Sancho ◽  
Pilar Campins-Falcó ◽  
María Dolores Pinazo-Durán ◽  
Cristina Peris-Martínez
Keyword(s):  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Mean Difference ◽  
The Other ◽  
Control Group ◽  
Healthy Population ◽  
Diagnostic Categories ◽  
Significant Difference ◽  
Biomechanical Parameters ◽  
The Mean

Purpose: To evaluate and compare corneal hysteresis (CH), corneal resistance factor (CRF), and central corneal thickness (CCT), measurements were taken between a healthy population (controls), patients diagnosed with glaucoma (DG), and glaucoma suspect patients due to ocular hypertension (OHT), family history of glaucoma (FHG), or glaucoma-like optic discs (GLD). Additionally, Goldmann-correlated intraocular pressure (IOPg) and corneal-compensated IOP (IOPcc) were compared between the different groups of patients. Methods: In this prospective analytical-observational study, a total of 1065 patients (one eye of each) were recruited to undergo Ocular Response Analyzer (ORA) testing, ultrasound pachymetry, and clinical examination. Corneal biomechanical parameters (CH, CRF), CCT, IOPg, and IOPcc were measured in the control group (n = 574) and the other groups: DG (n = 147), FHG (n = 78), GLD (n = 90), and OHT (n = 176). We performed a variance analysis (ANOVA) for all the dependent variables according to the different diagnostic categories with multiple comparisons to identify the differences between the diagnostic categories, deeming p < 0.05 as statistically significant. Results: The mean CH in the DG group (9.69 mmHg) was significantly lower compared to controls (10.75 mmHg; mean difference 1.05, p < 0.001), FHG (10.70 mmHg; mean difference 1.00, p < 0.05), GLD (10.63 mmHg; mean difference 0.93, p < 0.05) and OHT (10.54 mmHg; mean difference 0.84, p < 0.05). No glaucoma suspects (FHG, GLD, OHT groups) presented significant differences between themselves and the control group (p = 1.00). No statistically significant differences were found in the mean CRF between DG (11.18 mmHg) and the control group (10.75 mmHg; mean difference 0.42, p = 0.40). The FHG and OHT groups showed significantly higher mean CRF values (12.32 and 12.41 mmHg, respectively) than the DG group (11.18 mmHg), with mean differences of 1.13 (p < 0.05) and 1.22 (p < 0.001), respectively. No statistically significant differences were found in CCT in the analysis between DG (562 μ) and the other groups (control = 556 μ, FHG = 576 μ, GLD = 569 μ, OHT = 570 μ). The means of IOPg and IOPcc values were higher in the DG patient and suspect groups than in the control group, with statistically significant differences in all groups (p < 0.001). Conclusion: This study presents corneal biomechanical values (CH, CRF), CCT, IOPg, and IOPcc for diagnosed glaucoma patients, three suspected glaucoma groups, and a healthy population, using the ORA. Mean CH values were markedly lower in the DG group (diagnosed with glaucoma damage) compared to the other groups. No significant difference was found in CCT between the DG and control groups. Unexpectedly, CRF showed higher values in all groups than in the control group, but the difference was only statistically significant in the suspect groups (FHG, GLD, and OHT), not in the DG group.

Sign in / Sign up

Export Citation Format

Share Document