Genetic variability of Passiflora spp. from commercial fields in the Federal District, Brazil

This study aimed to characterize the genetic variability in commercial accessions of passion fruit from the Federal District, Brazil, by RAPD markers. Genetic analyses were done with leaf samples of 30 accessions. DNA samples were amplified by RAPD technique, and respective markers converted into a binary matrix, from which the genetic distances between the accessions were estimated. Clustering analyis based on genetic distances allowed to detect a wide range of genetic variabillity among the accessions of sour passion fruit, and to separate them from the two sweet passion fruit. The graphical positioning of 'BRS Ouro Vermelho' confirms its potential to improve the genetic variability of commercial varieties of sour passion fruit. Dispersal of genetic distances among commercial accessions of sour passion fruit supports evidence for different genetic origins of the materials planted in the Federal District. The verified genetic variability indicates the potential success of future breeding programs for this region.

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Genetic characterization of the Moxotó goat breed using RAPD markers

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2005000300006 ◽

2005 ◽

Vol 40 (3) ◽

pp. 233-239 ◽

Cited By ~ 12

Author(s):

Rejane Rodrigues de Oliveira ◽

Andréa Alves do Egito ◽

Maria Norma Ribeiro ◽

Samuel Rezende Paiva ◽

Maria do Socorro Maués Albuquerque ◽

...

Keyword(s):

Genetic Variability ◽

Rapd Markers ◽

Genetic Characterization ◽

Random Amplified Polymorphic Dna ◽

Genetic Distances ◽

Goat Breed ◽

Breeding Programs ◽

Analysis Of Molecular Variance ◽

Polymorphic Bands

The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.

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Genetic Variability of Passion Fruit Multispecific Hybrids and Their Respective Wild Parents Determined by Microsatellite Markers

Journal of Agricultural Science ◽

10.5539/jas.v11n10p302 ◽

2019 ◽

Vol 11 (10) ◽

pp. 302

Author(s):

Mara Cecília de Mattos Grisi ◽

Fábio Gelape Faleiro ◽

Nilton Tadeu Vilela Junqueira ◽

Jamile da Silva Oliveira

Keyword(s):

Genetic Variability ◽

Microsatellite Markers ◽

Genetic Distances ◽

Passion Fruit ◽

Group Method ◽

Breeding Programs ◽

Promising Alternative ◽

Pair Group ◽

Principal Coordinates ◽

Wild Accessions

The Passiflora genus comprises more than 500 species that are used for food, industrial, ornamental, and pharmaceutical purposes. The sour passion fruit (P. edulis Sims) has low genetic variability for disease resistance, and the use of wild species in the cross-breeding basis is a promising alternative for introgression of resistance genes. The objective of this study was to characterize multispecific hybrids and wild materials with potential to be used as parents in passion fruit genetic breeding programs, using microsatellite markers. Genomic DNA from 33 accessions was extracted and analyzed using 23 microsatellite markers, which were used to estimate the genetic dissimilarities among accessions. The genetic dissimilarity matrices were used to perform clustering analysis by dendrogram using the Unweighted Pair-Group Method as grouping criterion and by graphic dispersion based on multidimensional scale, using the principal coordinates method. Genetic distances between accessions ranged from 0.067 to 1.00. The markers indicated genetic variability among the studied accessions and also the efficiency of the recurrent genome recovery within the backcross program. The genetic structure among the accessions shows the clustering tendency between the wild accessions of P. hatschbachii and P. quadrifaria and the accessions obtained by crossing these species. The same occurred for P. incarnata and P. edulis accessions. The knowledge generated by the molecular characterization provides information on the diversity of accessions and contributes to the work of breeders in the selection of parents.

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Phenology of the production of flowers and fruits of wild and hybrid species of the genus Passiflora

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452018188 ◽

2018 ◽

Vol 40 (2) ◽

Cited By ~ 1

Author(s):

Danilo Akio de Sousa Esashika ◽

Fábio Gelape Faleiro ◽

Nilton Tadeu Vilela Junqueira

Keyword(s):

Central Region ◽

Wild Species ◽

High Potential ◽

Passion Fruit ◽

Brazilian Savanna ◽

Breeding Programs ◽

Hybrid Species ◽

Floral Buds ◽

Sour Passion Fruit

Abstract Wild species of passion fruit have characteristics that could be used in the passiflora culture, among them the production of fruits in the off-season of the sour passion fruit. The objective of this work was to evaluate the phenology of flowers and fruits production in wild species cultivated in the Brazilian Savanna Central Region. For each access, a 1-meter-long strip (2 m2) was evaluated for the presence of flowers and fruits from January 2015 to December 2016. The hybrid and wild species of passiflora that produce floral buds, flowers and fruits during the off-season of passion fruit sour include: BRS Céu do Cerrado (P. incarnata x P. edulis), P. alata Dryand, P. bahiensis Klotzsch, P. coccinea Aubl., P. maliformis L., P. quadriglandulosa Rodschied, P. auriculataKunth, P. decaisneana G. Nicholson, P. sidifolia M. Roem., P. suberosa L., P. tholozanii Sacco, P. quadrangularis x P. alata. It was also verified the presence of species and hybrids with high ornamental potential due to the beauty of their flowers and their production during the whole year, such as: P. suberosa L., P. tholozanii Sacco, P. coccinea x P. setacea, P. coccinea x P. quadrifaria. These species and hybrids have high potential for passiflora breeding programs as a source of genes of interest.

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Genotypic selection of multispecific hybrids obtained through crosses between commercial Passiflora edulis and wild passiflora species

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452021963 ◽

2021 ◽

Vol 43 (1) ◽

Author(s):

Mara Cecília de Mattos Grisi ◽

Nilton Tadeu Vilela Junqueira ◽

Leo Duc Haa Carson Schwartzhaupt da Conceição ◽

Fábio Gelape Faleiro ◽

Marcelo Fideles Braga ◽

...

Keyword(s):

Block Design ◽

Fruit Weight ◽

Passion Fruit ◽

Simultaneous Increase ◽

Interspecific Crosses ◽

Breeding Programs ◽

Passiflora Edulis ◽

Interspecific Introgression ◽

Randomized Block Design ◽

Sour Passion Fruit

Abstract The interspecific introgression of resistance genes to diseases in commercial varieties through interspecific crosses has been adopted as a strategy in breeding programs of sour passion fruit. This work aimed to evaluate 11 progenies of multispecific hybrids obtained from crosses involving seven Passiflora species, in addition to four commercial cultivars, using the REML/BLUP method. The experiment was carried out as a randomized block design with six repetitions and three plants per plot. Ten fruit traits were evaluated. The additive, multiplicative, and sum of ranks indexes were applied to determine the most appropriate selective strategy in the simultaneous increase of fruit weight, number of fruits, and yield. Plants from the 325 x VAO progeny showed a lower bacterial defoliation index and better means predicted for the traits number of fruits and productivity. The progenies 325 x LD4, PL3 x LD4, and the controls CSB-Marília and BRS-Gigante Amarelo showed plants with better-predicted means for traits related to fruit quality. The genetic gains demonstrated good prospects for the use of wild species in sour passion fruit genetic breeding.

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Variability and genetic structure of yellow passion fruit (Passiflora edulis f. flavicarpa Degener) in Colombia using microsatellite DNA markers

Agronomía Colombiana ◽

10.15446/agron.colomb.v35n2.59973 ◽

2017 ◽

Vol 35 (2) ◽

pp. 135-149 ◽

Cited By ~ 1

Author(s):

John Ocampo ◽

Natali Acosta-Barón ◽

Javier Hernández-Fernández

Keyword(s):

Genetic Variability ◽

Polymorphic Information Content ◽

Passion Fruit ◽

Hierarchical Level ◽

Breeding Programs ◽

Geographic Structure ◽

Microsatellite Dna Markers ◽

Superior Genotypes ◽

Valle Del Cauca ◽

Yellow Passion Fruit

Colombia is one of the leading producers of yellow passion fruit but the genetic studies based on molecular markers from commercial plantations have not been considered to select interesting market material. The goal of this study was to assess the genetic variability and the population structure of 51 Colombian commercial yellow passion fruit accessions (102 individuals), and to provide the necessary information for prospective selection and breeding programs. Thus, a total of six microsatellites were amplified with 58 alleles identified and an average of 9.66 alleles per locus, including nine private and 31 rare. Diversity indexes showed polymorphic information content values of 0.74 (PIC), an observed (Ho) and expected (He) heterozygosity average of 0.52 and 0.78, respectively. Spatial distribution showed the greatest allelic richness (11 to 14) in most of the Valle del Cauca accessions. The average genetic distance among accessions was 0.68, and the cluster analysis showed three main groups poorly supported (bootstrap <50%), with slight geographical structure and high differentiation between individuals of the same accession. Structure analysis indicated K=4 as the genetic structure's uppermost hierarchical level, while Bayesian clustering showed a division of individuals into four genetically distinct groups. The low geographic structure and high variability of the accessions could be explained by allogamy and seed exchange frequency among farmers. Results issued suggest a complementary agro-morphological assessment to establish total genetic variability and implement a breeding program through assisted selection of superior genotypes in search of more productive and resistant cultivars to phytosanitary problems.

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Molecular characterization and phylogeny of the entomopathogenic fungus

Plant Protection Science ◽

10.17221/9664-pps ◽

1999 ◽

Vol 35 (No. 1) ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

M. Oborník ◽

R. Stouthamer ◽

E. Meekes ◽

M. Schilthuittzen

Keyword(s):

Phylogenetic Analysis ◽

Genetic Variability ◽

Phylogenetic Tree ◽

Ribosomal Dna ◽

Dna Sequences ◽

Entomopathogenic Fungus ◽

Rapd Markers ◽

South India ◽

Geographical Origin ◽

Genetic Distances

We characterized 23 isolates of the entomopathogenic fungus Aschersonia spp. from Mexico, Brazil, Guyana, Trinidad, Venezuela, Columbia, Florida, Malaysia, Thailand, Japan, Philippines, Java and South India using RAPD markers. The data were used to compute the genetic variability and to reconstruct the phylogeny of the genus Aschersonia. Relative genetic distances varied from 0.018 (between isolates Aa2 and Ap2) to 0.445 (between isolates A1 and At1). In the constructed phylogenetic tree, isolates were clustered according to their geographical origin. We determined partial 26S ribosomal DNA sequences of five Aschersonia isolates (A28, A31, Ai1a, Ai2b – Aschersonia spp.; and Ap1– Aschersonia placenta) and used them for phylogenetic analysis. Three of the tested isolates were not distinguishable. The tree constructed indicated that isolates Ai1a and Ai2b belong to species distinct from A. placenta and A. aleyrodis.

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Genetic variability and phylogeny inferred from random amplified polymorphic DNA data reflect life strategy of entomopathogenic fungi

Canadian Journal of Botany ◽

10.1139/b00-087 ◽

2000 ◽

Vol 78 (9) ◽

pp. 1150-1155 ◽

Cited By ~ 5

Author(s):

Miroslav Oborník ◽

Marek Klíc ◽

Lukáš Zizka

Keyword(s):

Genetic Variability ◽

Entomopathogenic Fungi ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Genetic Distances ◽

Major Influence ◽

Life Strategy ◽

Verticillium Lecanii ◽

Paecilomyces Fumosoroseus ◽

Neighbor Joining

Our analysis of the genetic variability and phylogenetic relationships among the isolates of mitosporic entomopathogenic fungi Paecilomyces fumosoroseus (Wize) Brown & Smith (10 isolates), Paecilomyces farinosus (Holm ex S.F. Gray) Brown & Smith (3 isolates), Paecilomyces lilacinus (Thom) Samson (1 isolate), and Verticillium lecanii (Zimmerman) Viegas (6 isolates) was based on the use of random amplified polymorphic DNA (RAPD) markers. Neighbor-joining analysis characterized P. fumosoroseus and V. lecanii as monophyletic taxa. The analysis also supported monophyly of the genus Paecilomyces. We have used the distribution of genetic distances to demonstrate the genetic variability within studied species, in Paecilomyces spp. (Hyphomycetes) and Aschersonia spp. (Coelomycetes) in particular. We conclude that the RAPD-based analysis is useful for the reconstruction of intraspecific and interspecific relationships of the entomopathogenic fungi. Moreover, it can be employed to study the intergeneric relationships, especially in the case of closely related genera of the hyphomycetous entomopathogenic fungi. We propose that RAPD-based phylogeny and genetic variability reflect their life strategy. In our opinion, the mobility of spores represents a factor with major influence on the evolution of these entomopathogenic fungi.Key words: Paecilomyces, Verticillium, Aschersonia, RAPD, phylogeny, genetic variability.

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Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132010000200017 ◽

2010 ◽

Vol 53 (2) ◽

pp. 375-387 ◽

Cited By ~ 8

Author(s):

Luciana do Valle Rego Oliveira ◽

Ricardo Tadeu de Faria ◽

Claudete de Fátima Ruas ◽

Paulo Maurício Ruas ◽

Melissa de Oliveira Santos ◽

...

Keyword(s):

Molecular Markers ◽

Genetic Variability ◽

Genetic Analysis ◽

Genetic Relationship ◽

Genetic Similarity ◽

Core Collection ◽

Rapd Markers ◽

Dna Amplification ◽

Breeding Programs

In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.

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RAPD analysis of genetic variability in a multiprovenance base population of Eucalyptus grandis hill ex maiden

Revista Árvore ◽

10.1590/s0100-67622008000600001 ◽

2008 ◽

Vol 32 (6) ◽

pp. 961-967

Author(s):

Susi Meire Maximino Leite ◽

Édson Seizo Mori ◽

Celina Ferraz do Valle ◽

César Augusto Valencise Bonine ◽

Celso Luís Marino

Keyword(s):

Genetic Variability ◽

Genetic Similarity ◽

Statistical Tests ◽

Eucalyptus Grandis ◽

Base Population ◽

Binary Matrix ◽

Breeding Programs ◽

Genetic Breeding ◽

Two Factors ◽

Group 3

This study aimed to evaluate the genetic variability among individuals of a base population of Eucalyptus grandis and to build a molecular marker database for the analyzed populations. The Eucalyptus grandis base population comprised 327 individuals from Coff's Harbour, Atherton and Rio Claro. A few plants came from other sites (Belthorpe MT. Pandanus, Kenilworth, Yabbra, etc.). Since this base population had a heterogeneous composition, the groups were divided according to geographic localization (latitude and longitude), and genetic breeding level. Thus, the influence of those two factors (geographic localization and genetic breeding level) on the genetic variability detected was discussed. The RAPD technique allowed the evaluation of 70 loci. The binary matrix was used to estimate the genetic similarity among individuals using Jaccard's Coefficient. Parametric statistical tests were used to compare within-group similarity of the means. The obtained results showed that the base population had wide genetic variability and a mean genetic similarity of 0.328. Sub-group 3 (wild materials from the Atherton region) showed mean genetic similarity of 0.318. S.P.A. (from Coff's Harbour region) had a mean genetic similarity of 0.322 and was found to be very important for maintenance of variation in the base population. This can be explained since the individuals from those groups accounted for most of the base population (48.3% for it). The base population plants with genetic similarity higher than 0.60 should be phenotypically analyzed again in order to clarify the tendency of genetic variability during breeding programs.

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