RAPD analysis of genetic variability in a multiprovenance base population of Eucalyptus grandis hill ex maiden

This study aimed to evaluate the genetic variability among individuals of a base population of Eucalyptus grandis and to build a molecular marker database for the analyzed populations. The Eucalyptus grandis base population comprised 327 individuals from Coff's Harbour, Atherton and Rio Claro. A few plants came from other sites (Belthorpe MT. Pandanus, Kenilworth, Yabbra, etc.). Since this base population had a heterogeneous composition, the groups were divided according to geographic localization (latitude and longitude), and genetic breeding level. Thus, the influence of those two factors (geographic localization and genetic breeding level) on the genetic variability detected was discussed. The RAPD technique allowed the evaluation of 70 loci. The binary matrix was used to estimate the genetic similarity among individuals using Jaccard's Coefficient. Parametric statistical tests were used to compare within-group similarity of the means. The obtained results showed that the base population had wide genetic variability and a mean genetic similarity of 0.328. Sub-group 3 (wild materials from the Atherton region) showed mean genetic similarity of 0.318. S.P.A. (from Coff's Harbour region) had a mean genetic similarity of 0.322 and was found to be very important for maintenance of variation in the base population. This can be explained since the individuals from those groups accounted for most of the base population (48.3% for it). The base population plants with genetic similarity higher than 0.60 should be phenotypically analyzed again in order to clarify the tendency of genetic variability during breeding programs.

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Genetic variability of Passiflora spp. from commercial fields in the Federal District, Brazil

Ciência Rural ◽

10.1590/s0103-84782011000600013 ◽

2011 ◽

Vol 41 (6) ◽

pp. 996-1002 ◽

Cited By ~ 6

Author(s):

Ana Paula Gomes de Castro ◽

Fábio Gelape Faleiro ◽

Daniel Diego Costa Carvalho ◽

Kenia Gracielle da Fonseca ◽

Marina de Fátima Vilela ◽

...

Keyword(s):

Genetic Variability ◽

Rapd Markers ◽

Federal District ◽

Genetic Distances ◽

Passion Fruit ◽

Binary Matrix ◽

Breeding Programs ◽

Wide Range ◽

Potential Success ◽

Sour Passion Fruit

This study aimed to characterize the genetic variability in commercial accessions of passion fruit from the Federal District, Brazil, by RAPD markers. Genetic analyses were done with leaf samples of 30 accessions. DNA samples were amplified by RAPD technique, and respective markers converted into a binary matrix, from which the genetic distances between the accessions were estimated. Clustering analyis based on genetic distances allowed to detect a wide range of genetic variabillity among the accessions of sour passion fruit, and to separate them from the two sweet passion fruit. The graphical positioning of 'BRS Ouro Vermelho' confirms its potential to improve the genetic variability of commercial varieties of sour passion fruit. Dispersal of genetic distances among commercial accessions of sour passion fruit supports evidence for different genetic origins of the materials planted in the Federal District. The verified genetic variability indicates the potential success of future breeding programs for this region.

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GENETIC VARIABILITY IN PROGENIES OF Eucalyptus grandis AND Eucalyptus urophylla FOR TOLERANCE TO HYDRIC DEFICIT

FLORESTA ◽

10.5380/rf.v48i4.56213 ◽

2018 ◽

Vol 48 (4) ◽

pp. 543 ◽

Cited By ~ 1

Author(s):

Mateus Chagas ◽

Leandro Siqueira ◽

Shinitiro Oda ◽

Raimundo Medeiros ◽

Evandro Vagner Tambarussi

Keyword(s):

Genetic Variability ◽

Block Design ◽

Eucalyptus Grandis ◽

Eucalyptus Urophylla ◽

Forest Expansion ◽

Breeding Programs ◽

Genetic Gains ◽

Randomized Block Design ◽

Genotypic And Phenotypic Correlations ◽

Coefficient Of Genetic Variation

Genetic variability in progenies of Eucalyptus grandis and Eucalyptus urophylla for tolerance to hydric deficit. Information on the adaptation and genetic potential of Eucalyptus under water deficit conditions is necessary owing to the increasing cultivation of the genus in regions considered as "forest frontiers". Therefore, the present study aimed to estimate genetic variability, genotypic and phenotypic correlations, and estimated genetic gains in progenies of E. grandis and E. urophylla. Two progeny tests (TP1 and TP2) were performed in the municipality of Vila Nova dos Martirios (state of Maranhão, Brazil), in 30-month-old plants. The experiment was carried out using a randomized block design, with five replicates, nine plants/square plot, and 144 treatments in TP1 and 264 in TP2. Statistical analysis was performed and genetic parameters were estimated. Individual and within-progeny heritabilities were corrected using the kinship coefficient for eachspecies. Genetic gains were low, ranging from 0.96 to 6.39% for E. grandis and 1.62 to 5.48% for E. urophylla. The diameter at breast height (DBH) showed a higher coefficient of genetic variation compared with the other measured characteristics, suggesting a higher potential for selection. High, positive, and significant correlations were observed between DBH and height (H), indicating the possibility of obtaining gains based on a single characteristic. Heritability was considered low, thus directly influencing the selection gains, which were lower than those commonly reported in other studies. These findings provide important information for breeding programs in areas of forest expansion.

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Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132010000200017 ◽

2010 ◽

Vol 53 (2) ◽

pp. 375-387 ◽

Cited By ~ 8

Author(s):

Luciana do Valle Rego Oliveira ◽

Ricardo Tadeu de Faria ◽

Claudete de Fátima Ruas ◽

Paulo Maurício Ruas ◽

Melissa de Oliveira Santos ◽

...

Keyword(s):

Molecular Markers ◽

Genetic Variability ◽

Genetic Analysis ◽

Genetic Relationship ◽

Genetic Similarity ◽

Core Collection ◽

Rapd Markers ◽

Dna Amplification ◽

Breeding Programs

In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.

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Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210635 ◽

2020 ◽

Vol 21 (6) ◽

Author(s):

KARIMA KARA ◽

MALIKA RACHED-KANOUNI ◽

SAMAH MNASRI ◽

Hichem Khammar ◽

M’BAREK BEN NACEUR

Keyword(s):

Triticum Aestivum ◽

Genetic Variability ◽

Bread Wheat ◽

Ssr Markers ◽

Genetic Similarity ◽

Triticum Aestivum L ◽

Breeding Programs ◽

High Level ◽

Derived Data ◽

Selection Of

Abstract. Kara K, Rached-Kanouni M, Mnasri S, Khammar H, Ben Naceur MB. 2020. Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers. Biodiversitas 21: 2638-2644. The assessment of genetic diversity is a key step in evaluating the adaptation of populations to new environmental conditions, and thus in the selection of new cultivars. The present investigation aimed to assess genetic variability of bread wheat (Triticum aestivum L.) genotypes grown in Algeria. The 17 hexaploid genotypes of bread wheat were assessed using 16 molecular microsatellites of SSR (Simple Sequence Repeat). Among the 16 microsatellite markers tested, only 11 markers were the most polymorphic and reproducible. The Polymorphism Information Content (PIC) values per locus varied from 0.14 to 0.70 with an average of 0.48 and 0.49. Genetic similarity between genotypes varied from 0.27 and 0.92 with an average of 0.60. The highest genetic distance value of 0.92 has been scored between Kauz/Pastor/Fiscal and Wbll1*2/Brambling. The lowest value of 0.27 has been scored between Cham6 and Pastor/Wbll1. Genetic similarity was calculated by molecular derived data and used to produce a dendrogram. The genotypes were grouped in two clear clusters according to their origin and pedigree. The first cluster included wheat genotypes Ain Abid (local genotypes), Attila/2 Pastor, and 5119 (introduced genotypes). The second group revealed high polymorphism and was subdivided into four sub-groups. This high level of diversity revealed among the accessions of wheat, grown in Algeria could be used in breeding programs.

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Synthesis of a base population of Habanero pepper

Horticultura Brasileira ◽

10.1590/s0102-053620150000400021 ◽

2015 ◽

Vol 33 (4) ◽

pp. 530-532 ◽

Cited By ~ 4

Author(s):

Luciano L Nass ◽

Karina RR Souza ◽

Claudia SC Ribeiro ◽

Francisco JB Reifschneider

Keyword(s):

Genetic Variability ◽

Recurrent Selection ◽

Inbred Lines ◽

Breeding Program ◽

Base Population ◽

Germplasm Bank ◽

Breeding Programs ◽

Fixed Weight ◽

Habanero Pepper ◽

Different Origins

ABSTRACT: The establishment of populations with ample genetic variability and their use in breeding programs are discussed based on the Capsicum breeding program at Embrapa. Thirty-one accessions of "Habanero" pepper from different origins present in the Capsicum Active Germplasm Bank (AGB) of Embrapa Vegetables were used to establish a base population. The base population of "Habanero" was formed by an equal mixture of F2 seeds from all crosses obtained, using a fixed weight (1 g) per cross, which is close to 150 seeds per genotype. This balanced population was introduced in theCapsicum AGB as CNPH 15,469. There are several possibilities to explore this variability, such as the use of base population as source of inbred lines; the use of base population for selection in specific environments; establishment of new populations from the base population, and also its use in recurrent selection programs.

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Characterization of genetic variability among common bean genotypes by morphological descriptors

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332012000100010 ◽

2012 ◽

Vol 12 (1) ◽

pp. 76-84 ◽

Cited By ~ 4

Author(s):

Marilene Santos de Lima ◽

José Eustáquio de Souza Carneiro ◽

Pedro Crescêncio Souza Carneiro ◽

Camila Santana Pereira ◽

Rogério Faria Vieira ◽

...

Keyword(s):

Genetic Variation ◽

Genetic Variability ◽

Common Bean ◽

Genetic Divergence ◽

Genetic Similarity ◽

Morphological Descriptors ◽

Germplasm Bank ◽

Breeding Programs ◽

Genetic Dissimilarity

The purpose of this study was to characterize the genetic variability in 100 genotypes of the Active Germplasm Bank of common bean of the Federal University of Viçosa, by morphological descriptors, classify them in groups of genetic similarity and to identify the degree of relevance of descriptors of genetic divergence. The genotypes were evaluated based on 22 quantitative and qualitative morphological descriptors. The high-yielding genotypes V 7936, Gold Gate, LM 95103904, 1829 S 349 Venezuela, and PF 9029975, CNFC 9454 and Fe 732015, with upright growth, have potential for use as parents in common bean breeding programs. By genetic divergence analysis, the genotypes were clustered in eight groups of genetic dissimilarity. By methods of principal components, 9 of the 22 descriptors were eliminated, for being redundant or little variable, suggesting that 10-20 morphological descriptors can be used in studies of characterization of genetic variation.

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ESTIMASI NILAI HERITABILITAS BOBOT IKAN MAS VARIETAS PUNTEN DALAM PROGRAM SELEKSI INDIVIDU

Jurnal Riset Akuakultur ◽

10.15578/jra.11.3.2016.217-223 ◽

2016 ◽

Vol 11 (3) ◽

pp. 217

Author(s):

Estu Nugroho ◽

Budi Setyono ◽

Mochammad Su’eb ◽

Tri Heru Prihadi

Keyword(s):

Body Weight ◽

Selection Response ◽

Base Population ◽

Breeding Programs ◽

Male And Female ◽

Body Weight Trait ◽

Population Selection ◽

Selection For ◽

Weight Trait ◽

Selection Of

Program pemuliaan ikan mas varietas Punten dilakukan dengan seleksi individu terhadap karakter bobot ikan. Pembentukan populasi dasar untuk kegiatan seleksi dilakukan dengan memijahkan secara massal induk ikan mas yang terdiri atas 20 induk betina dan 21 induk jantan yang dikoleksi dari daerah Punten, Kepanjen (delapan betina dan enam jantan), Kediri (tujuh betina dan 12 jantan), Sragen (27 betina dan 10 jantan), dan Blitar (15 betina dan 11 jantan). Larva umur 10 hari dipelihara selama empat bulan. Selanjutnya dilakukan penjarangan sebesar 50% dan benih dipelihara selama 14 bulan untuk dilakukan seleksi dengan panduan hasil sampling 250 ekor individu setiap populasi. Seleksi terhadap calon induk dilakukan saat umur 18 bulan pada populasi jantan dan betina secara terpisah dengan memilih berdasarkan 10% bobot ikan yang terbaik. Calon induk yang terseleksi kemudian dipelihara hingga matang gonad, kemudian dipilih sebanyak 150 pasang dan dipijahkan secara massal. Didapatkan respons positif dari hasil seleksi berdasarkan bobot ikan, yaitu 49,89 g atau 3,66% (populasi ikan jantan) dan 168,47 g atau 11,43% (populasi ikan betina). Nilai heritabilitas untuk bobot ikan adalah 0,238 (jantan) dan 0,505 (betina).Punten carp breeding programs were carried out by individual selection for body weight trait. The base population for selection activities were conducted by mass breeding of parent consisted of 20 female and 21 male collected from area Punten, eight female and six male (Kepanjen), seven female and 12 male (Kediri), 27 female and 10 male (Sragen), 15 female and 11 male (Blitar). Larvae 10 days old reared for four moths. Then after spacing out 50% of total harvest, the offspring reared for 14 months for selection activity based on the sampling of 250 individual each population. Selection of broodstock candidates performed since 18 months age on male and female populations separately by selecting based on 10% of fish with best body weight. Candidates selected broodstocks were then maintained until mature. In oder to produce the next generation 150 pairs were sets and held for mass spawning. The results revealed that selection response were positive, 49.89 g (3.66%) for male and 168.47 (11.43%) for female. Heritability for body weight is 0.238 (male) and 0.505 (female).

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Genetic variability and genome-wide association analysis of flavor and texture in cooked beans (Phaseolus vulgaris L.)

Theoretical and Applied Genetics ◽

10.1007/s00122-020-03745-3 ◽

2021 ◽

Author(s):

Amber Bassett ◽

Kelvin Kamfwa ◽

Daniel Ambachew ◽

Karen Cichy

Keyword(s):

Genetic Variability ◽

Seed Coat ◽

Principal Component ◽

Breeding Programs ◽

Flavor Intensity ◽

Genome Wide ◽

White Bean ◽

The Usa ◽

New Varieties ◽

End Use

Abstract Key message Cooked bean flavor and texture vary within and across 20 Andean seed types; SNPs are significantly associated with total flavor, beany, earthy, starchy, bitter, seed-coat perception, and cotyledon texture. Abstract Common dry beans are a nutritious food recognized as a staple globally, but their consumption is low in the USA. Improving bean flavor and texture through breeding has the potential to improve consumer acceptance and suitability for new end-use products. Little is known about genetic variability and inheritance of bean sensory characteristics. A total of 430 genotypes of the Andean Diversity Panel representing twenty seed types were grown in three locations, and cooked seeds were evaluated by a trained sensory panel for flavor and texture attribute intensities, including total flavor, beany, vegetative, earthy, starchy, sweet, bitter, seed-coat perception, and cotyledon texture. Extensive variation in sensory attributes was found across and within seed types. A set of genotypes was identified that exhibit extreme attribute intensities generally stable across all three environments. seed-coat perception and total flavor intensity had the highest broad-sense heritability (0.39 and 0.38, respectively), while earthy and vegetative intensities exhibited the lowest (0.14 and 0.15, respectively). Starchy and sweet flavors were positively correlated and highest in white bean genotypes according to principal component analysis. SNPs associated with total flavor intensity (six SNPs across three chromosomes), beany (five SNPs across four chromosomes), earthy (three SNPs across two chromosomes), starchy (one SNP), bitter (one SNP), seed-coat perception (three SNPs across two chromosomes), and cotyledon texture (two SNPs across two chromosomes) were detected. These findings lay a foundation for incorporating flavor and texture in breeding programs for the development of new varieties that entice growers, consumers, and product developers alike.

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Genetic Similarity of Avena sativa L. Varieties as an Example of a Narrow Genetic Pool of Contemporary Cereal Species

Plants ◽

10.3390/plants10071424 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1424

Author(s):

Magdalena Cieplak ◽

Sylwia Okoń ◽

Krystyna Werwińska

Keyword(s):

Genetic Diversity ◽

Avena Sativa ◽

Genetic Similarity ◽

Breeding Programs ◽

Central European ◽

Cereal Species ◽

Genetic Pool ◽

Selection Of ◽

High Genetic Similarity

The assessment of the genetic diversity of cultivated varieties is a very important element of breeding programs. This allows the determination of the level of genetic differentiation of cultivated varieties, their genetic distinctiveness, and is also of great importance in the selection of parental components for crossbreeding. The aim of the present study was to determine the level of genetic diversity of oat varieties currently grown in Central Europe based on two marker systems: ISSR and SCoT. The research conducted showed that both these types of markers were suitable for conducting analyses relating to the assessment of genetic diversity. The calculated coefficients showed that the analyzed cultivars were characterized by a high genetic similarity. However, the UPGMA and PCoA analyses clearly indicated the distinctiveness of the breeding programs conducted in Central European countries. The high genetic similarity of the analyzed forms allow us to conclude that it is necessary to expand the genetic pool of oat varieties. Numerous studies show that landraces may be the donor of genetic variation.

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