scholarly journals Inulinase from Kluyveromyces marxianus: culture medium composition and enzyme extraction

1999 ◽  
Vol 16 (3) ◽  
pp. 237-245 ◽  
Author(s):  
A. PESSOA JR ◽  
M. VITOLO
Keyword(s):  
Kluyveromyces Marxianus ◽  
Culture Medium ◽  
Medium Composition ◽  
Enzyme Extraction

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Differentiation of mesenchymal stem cells from humans and animals into insulin-producing cells: An overview in vitro induction forms

2020 ◽  
Vol 16 ◽  
Author(s):  
Bruna O. S. Câmara ◽  
Bruno M. Bertassoli ◽  
Natália M. Ocarino ◽  
Rogéria Serakides
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture Medium ◽  
Adipogenic Differentiation ◽  
Medium Composition ◽  
Cell Therapies ◽  
Insulin Producing Cells ◽  
Msc Differentiation

The use of stem cells in cell therapies has shown promising results in the treatment of several diseases, including diabetes mellitus, in both humans and animals. Mesenchymal stem cells (MSCs) can be isolated from various locations, including bone marrow, adipose tissues, synovia, muscles, dental pulp, umbilical cords, and the placenta. In vitro, by manipulating the composition of the culture medium or transfection, MSCs can differentiate into several cell lineages, including insulin-producing cells (IPCs). Unlike osteogenic, chondrogenic, and adipogenic differentiation, for which the culture medium and time are similar between studies, studies involving the induction of MSC differentiation in IPCs differ greatly. This divergence is usually evident in relation to the differentiation technique used, the composition of the culture medium, the cultivation time, which can vary from a few hours to several months, and the number of steps to complete differentiation. However, although there is no “gold standard” differentiation medium composition, most prominent studies mention the use of nicotinamide, exedin-4, ß-mercaptoethanol, fibroblast growth factor b (FGFb), and glucose in the culture medium to promote the differentiation of MSCs into IPCs. Therefore, the purpose of this review is to investigate the stages of MSC differentiation into IPCs both in vivo and in vitro, as well as address differentiation techniques and molecular actions and mechanisms by which some substances, such as nicotinamide, exedin-4, ßmercaptoethanol, FGFb, and glucose, participate in the differentiation process.

scholarly journals Efficiency of using different types induction culture medium for hexaploid triticale anther cultivation

2019 ◽  
Vol 25 ◽  
pp. 260-265
Author(s):  
E. V. Lagunovskaya ◽  
O. I. Zaitseva ◽  
V. A. Lemesh
Keyword(s):  
Culture Medium ◽  
Medium Composition ◽  
Induction Medium ◽  
Hexaploid Triticale ◽  
Culture Methods ◽  
Study Results ◽  
Short Period ◽  
Androgenesis In Vitro ◽  
The Republic

Aim. Triticale is one of the main grain crops of the Republic of Belarus. Further progress in the selection of this culture involves the accelerated creation of highly productive early ripening varieties resistant to abiotic and biotic factors. The method of induced androgenesis in vitro makes it possible to obtain stable homozygous lines in a short period of time and to eliminate the lengthy process of inbreeding used in classical breeding to fix the desired traits. Methods. The tissue and cell culture methods for plants was used in the study. Results. The influence of the induction medium composition on the efficiency of in vitro induced androgenesis in varieties and lines of hexaploid triticale is assessed. The influence of three types of induction culture medium, the type of phytohormones and the presence or absence of cefotaxime in the medium are analyzed. Results. It has been shown that using the C-17 culture medium supplemented with 2.0 mg/l 2,4-D and 0.5 mg/l kinetin without adding cefotaxime is most effective for the anther triticale cultivation. Keywords: triticale, anther culture, induction nutrient medium, embryoids, calli, regenerant plants, cefotaxime.

scholarly journals Handling culture medium composition for optimizing plant cell suspension culture in shake flasks

2017 ◽  
Vol 133 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Tiago Fidemann ◽  
Gabriela Aparecida de Araujo Pereira ◽  
Tárik Reis Heluy ◽  
Rodrigo Boccoli Gallego ◽  
Mônica Rosa Bertão ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Culture Medium ◽  
Medium Composition ◽  
Plant Cell Suspension Culture ◽  
Plant Cell Suspension ◽  
Shake Flasks

scholarly journals Fungal Exopolysaccharide: Production, Composition and Applications

2013 ◽  
Vol 6 ◽  
pp. MBI.S10957 ◽  
Author(s):  
Subhadip Mahapatra ◽  
Debdulal Banerjee
Keyword(s):  
Molecular Weight ◽  
Culture Medium ◽  
Medium Composition ◽  
General Outline ◽  
Exopolysaccharide Production ◽  
Fungal Strains ◽  
Physical Conditions ◽  
Extensive Search ◽  
Production Composition

Fungal exopolysaccharides (EPSs) have been recognized as high value biomacromolecules for the last two decades. These products, including pullulan, scleroglucan, and botryosphaeran, have several applications in industries, pharmaceuticals, medicine, foods etc. Although fungal EPSs are highly relevant, to date information concerning fungal biosynthesis is scarce and an extensive search for new fugal species that can produce novel EPSs is still needed. In most cases, the molecular weight variations and sugar compositions of fungal EPSs are dependent to culture medium composition and different physical conditions provided during fermentation. An inclusive and illustrative review on fungal EPS is presented here. The general outline of the present work includes fungal EPS production, their compositions and applications. An emphasis is also given to listing out different fungal strains that can produce EPSs.

Effects of an Electric Field of Sinusoidal Waves on the Amino Acid Biosynthesis by Azotobacter

1980 ◽  
Vol 35 (3-4) ◽  
pp. 258-261
Author(s):  
A. Martin Gonzalez ◽  
M. T. Izquierdo
Keyword(s):  
Amino Acids ◽  
Nitrogen Fixation ◽  
Amino Acid ◽  
Electric Field ◽  
Electric Fields ◽  
Culture Medium ◽  
Azotobacter Vinelandii ◽  
Amino Acid Biosynthesis ◽  
Medium Composition ◽  
Acid Biosynthesis

Abstract Electric Field Electric fields of sinusoidal waves have been applied in cultures of Azotobacter vinelandii, with potentials between 0 V and 10 V, intensities from 0 mA to 16 mA and frequencies between 5 Hz and 200 KHz. The influence of the electric field of sinusoidal waves on the nitrogen fixation on the post­ culture medium composition has a maximum at 5 V, 8 mA and 20 Hz. The rate of synthesis of specific amino acids by Azotobacter depends on the frequency and potential of the electric field applied. The concentration of each amino acid present in the post-culture medium is increased according to the electric field employed and the amino acid biosynthesis in culture medium is activated during the first days of incubation.

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