scholarly journals GENETIC DIVERSITY IN ARABICA COFFEE GROWN IN POTASSIUM-CONSTRAINED ENVIRONMENT

2015 ◽  
Vol 39 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Waldênia de Melo Moura ◽  
Yaska Janaína Bastos Soares ◽  
Antônio Teixeira do Amaral Júnior ◽  
Paulo César de Lima ◽  
Hermínia Emília Prieto Martinez ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Clustering Algorithm ◽  
Production Systems ◽  
Block Design ◽  
Group Method ◽  
Breeding Programs ◽  
Canonical Variables ◽  
Pair Group ◽  
Randomized Block Design ◽  
Renewable Natural Resource

Potassium is a source of non-renewable natural resource, and is used in large quantities in coffee fertilization through basically imported formulations in the form of potassium chloride. An alternative to make production systems more sustainable would be obtaining cultivars more efficient in the use of this nutrient. This study aimed to evaluate the genetic diversity among 20 cultivars of coffee, in conditions of low availability of potassium to identify the best combinations for composing future populations to be used in breeding programs. The experiment was arranged in a randomized block design with three replications of nutrient solution. Agronomic characteristics and efficiencies of rooting, absorption, translocation, biomass production and potassium utilization were evaluated. The clustering analysis was based on the unweighted pair group method with arithmetic mean clustering algorithm (UPGMA) and canonical variables. Variability was observed for most treatments. The multivariate procedures produced similar discrimination of genotypes, with the formation of five groups. Hybridizations between the cultivar Icatu Precoce IAC 3283 with cultivars Catuaí Amarelo IAC 62, Araponga MG1, Caturra Vermelho IAC 477, Catuaí Vermelho IAC 15, Rubi MG 1192 and Catucaí 785/15, and between the cultivar Tupi IAC 1669-33 with cultivars Icatu Vermelho IAC 4045, Acaiá Cerrado MG 1474 and Oeiras MG 6851 are the most promising for obtaining segregating populations or heterotic hybrids in breeding programs aiming more efficiency in potassium utilization.

scholarly journals Components of resistance to Olivea neotectonae in Tectona grandis Linn F. as a criterion for selecting promising genotypes

2021 ◽  
pp. 835-841
Author(s):  
Antonio Marcos Chimello ◽  
Jeferson Gonçalves de Jesus ◽  
Suelene Surubi de Melo ◽  
Isabela Vera dos Anjos ◽  
Milson Evaldo Serafim ◽  
...  
Keyword(s):  
Block Design ◽  
Tectona Grandis ◽  
Group Method ◽  
Linear Unbiased Prediction ◽  
Canonical Variables ◽  
Pair Group ◽  
Progress Curve ◽  
Randomized Block Design ◽  
Improvement Programs ◽  
Best Linear Unbiased

The aim of this paper was to identify the best components involving resistance to the O. neotectonae fungus, allowing for adequate selection of promising teak genotypes to explore in improvement programs or for disease management. Thirty different clonal teak genotypes were evaluated in a greenhouse from PROTECA Biotecnologia Florestal. The experimental design was a randomized block design with three replications and three plants per plot. The following characteristics were evaluated: average latent period, number of pustules per cm2, area below the number of pustules progress curve (ABNPPC), frequency of infection, and number of urediniospores per pustule. The data for the resistance characteristics were submitted to ANOVA and multivariate analysis applying grouping techniques and canonical variables. High genetic variability was observed among the 30 T. grandis genotypes regarding resistance to the O. neotectonae fungus. Both for the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), the canonical variables method and Best Linear Unbiased Prediction (BLUP) the genotypes that showed the greatest resistance to the fungus were genotypes 03 and 10

scholarly journals Partial diallel and genetic divergence analyses in maize inbred lines

2020 ◽  
Vol 43 ◽  
pp. e53540
Author(s):  
Maria Fernanda de Souza Dias Maioli ◽  
Ronald José Barth Pinto ◽  
Tereza Aparecida da Silva ◽  
Diego Ary Rizzardi ◽  
Robson Akira Matsuzaki ◽  
...  
Keyword(s):  
Grain Yield ◽  
Genetic Divergence ◽  
Block Design ◽  
Inbred Lines ◽  
Group Method ◽  
Arithmetic Average ◽  
Pair Group ◽  
Maize Inbred Lines ◽  
Randomized Block Design ◽  
Partial Diallel

In this study, we aimed to estimate general and specific combining abilities (GCA and SCA, respectively) and to verify genetic divergence (Rogers distance, Unweighted Pair-Group Method Using Arithmetic Average - UPGMA) using microsatellite markers in maize inbred lines. Using a partial diallel scheme, a total of 19 inbred lines were crossed as (9 x 10), which were derived from the single hybrids SG6015 and P30F53, respectively. The 90 hybrids were evaluated in an incomplete randomized block design with common checks and three replications during the 2017-2018 growing season. Flowering time, average plant height, ear height insertion, average ear diameter, ear length, number of lodged and broken plants, mass of 100 grains and grain yield were measured. According to the analysis of variance, GCA, and SCA were significant (p < 0.05) in all the measured traits; inbred line B as well as 1 and 8, derived from the single hybrids SG6015 and P30F53, respectively, were selected due to their higher GCA values in grain yield to be used in crosses as testers, while the single cross hybrid (B x 1) was selected due to their higher SCA value in grain yield to be used in future breeding programs. The molecular marker analysis divided the inbred lines into two groups, where the highest dissimilarity (0.74) was observed between lines A and 9; however, these did not result in a high SCA value, therefore the hybrids obtained by such crossings were not selected for grain yield.

scholarly journals GENETIC DIVERSITY INAUS RICE LANDRACES OF BANGLADESH USING SSR MARKERS

2017 ◽  
Vol 30 (1) ◽  
pp. 11-20 ◽  
Author(s):  
M. Khalequzzaman ◽  
M. Z. Islam ◽  
M. A. Siddique ◽  
M. F. R. K. Prince ◽  
E. S. M. H. Rashid ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Three Dimensional ◽  
Group Method ◽  
Breeding Programs ◽  
Arithmetic Average ◽  
Pair Group ◽  
Rice Landraces ◽  
Conservation Genetic ◽  
Maximum Selection

Assessment of genetic diversity is essential for germplasm characterization, utilization and conservation. Genetic diversity of 31 Aus rice landraces of Bangladesh was assessed using 36 SSR (simple sequence repeats) markers. A total of 141 alleles were detectedand the number of alleles per locus ranged from two (RM1216, RM145, RM282, RM293, RM567and RM496) to 10 alleles (RM304), with an average of 3.92. The gene diversity varied from 0.06 (RM145) to 0.80 (RM304) with an average of 0.54 and the PIC values ranged from 0.06 (RM145) to 0.78 (RM304), with an average of 0.48.PIC value revealed that RM304 was the best marker for characterizing the studied Aus rice genotypes. The dendrogram from unweighted pair-group method with arithmetic average clustering of markers classified the genotypes into five major groups with a coefficient of 0.49. Two and three-dimensional graphical views of Principal Coordinate Analysis (PCA) revealed that the genotypes Hashikalmi, Chaina, Puitraaijang, Saithsail, Kuchmuch, Kalodhan, Ausdhan and Itcriewere found far away from the centroid of the cluster and can be selected as parents for further breeding programs.The results provided some useful implications for establishment of sovereignty of Bangladeshi rice gene pool. This information will provide maximum selection of diverse parents, background selection during backcross breeding programs and assist in broadening germplasm-based rice breeding programs in future.

scholarly journals Description and analysis of genetic diversity among squash accessions

2009 ◽  
Vol 52 (2) ◽  
pp. 271-283 ◽  
Author(s):  
Athanasios L. Tsivelikas ◽  
Olga Koutita ◽  
Anastasia Anastasiadou ◽  
George N. Skaracis ◽  
Ekaterini Traka-Mavrona ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Clustering Algorithm ◽  
Random Amplified Polymorphic Dna ◽  
Principal Component ◽  
Molecular Data ◽  
Optimal Number ◽  
Group Method ◽  
Arithmetic Average ◽  
Pair Group

In this work, the part of the squash core collection, maintained in the Greek Gene Bank, was assessed using the morphological and molecular data. Sixteen incompletely classified accessions of the squash were characterized along with an evaluation of their resistance against two isolates of Fusarium oxysporum. A molecular analysis using Random Amplified Polymorphic DNA (RAPD) markers was also performed, revealing high level of polymorphism. To study the genetic diversity among the squash accessions, a clustering procedure using Unweighed Pair Group Method and Arithmetic Average (UPGMA) algorithm was also adopted. Two independent dendrograms, one for the morphophysiological and one for molecular data were obtained, classifying the accessions into two and three main clusters, respectively. Despite the different number of the clusters there were many similarities between these two dendrograms, and a third dendrogram resulting from their combination was also produced, based on Gower's distance and UPGMA clustering algorithm. In order to determine the optimal number of clusters, the upper tail approach was applied. The more reliable clustering of the accessions was accomplished using RAPD markers as well as the combination of the two different data sets, classifying the accessions into three significantly different groups. These groups corresponded to the three different cultivated species of C. maxima Duch., C. moschata Duch., and C. pepo L. The same results were also obtained using Principal Component Analysis.

scholarly journals Genetic Variability of 21 Tea Genotypes [Camellia sinensis (L.) O. Kuntze] Based on RAPD Markers

2018 ◽  
Vol 5 (2) ◽  
pp. 77
Author(s):  
Budi Martono ◽  
Syafaruddin Syafaruddin
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Similarity ◽  
Binary Data ◽  
Rapd Markers ◽  
Group Method ◽  
Breeding Programs ◽  
Pair Group ◽  
Monomorphic Band ◽  
And Cluster Analysis

<em>Knowing the genetic diversity in the tea germplasms collection is one of important conditions for assembling new superior varieties. Information of genetic diversity can be obtained through analysis using RAPD molecular markers. The study aimed to determine the genetic diversity of 21 tea genotypes based on RAPD markers. The research was conducted in Integrated Laboratory, Seameo Biotrop, Bogor, from July to September 2013. Genomic DNA was isolated from 21 tea genotypes leaf samples, then amplified with primer OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, and OPD 08. Electrophoresis result was converted into binary data. The genetic similarity and cluster analysis calculation was done using NTSYS-pc version 2.10. In this research, 50 polymorphic bands (94,34%) and 3 monomorphic band (5,66%) were obtained. Cluster analysis based on Nei's genetic distance using the unweighted pair-group method with arithmatic (UPGMA) divided 21 tea genotypes into two groups at a genetic similarity value of 0,48. Group 1 consisted of 20 tea genotypes, while the second group comprised only a one genotype (Sin 27). The range of genetic similarity matrix was between 28%–92%, the lowest genetic similarity (28%) was found between GMB 4 and Sin 27 genotypes, while the highest (92%) was found between AS 2 and AS 1 genotypes. The information obtained can be utilized in breeding programs with the support of agronomic characters as well as in the conservation of tea germplasm.</em>

scholarly journals EARLY GROWTH AND SEEDLING MORPHOLOGY OF SPECIES OF Sesbania Scop. (Leguminosae, robinieae)

1999 ◽  
Vol 56 (2) ◽  
pp. 329-335 ◽  
Author(s):  
Elisabeth Ann Veasey ◽  
Marta Organo Negrão ◽  
Paulo Sodero Martins ◽  
Gerhard Bandel
Keyword(s):  
Cluster Analysis ◽  
Intraspecific Variation ◽  
Block Design ◽  
Group Method ◽  
Seedling Morphology ◽  
Low Frequencies ◽  
Initial Development ◽  
Arithmetic Average ◽  
Pair Group ◽  
Randomized Block Design

The objective of this study was to characterize 17 accessions of Sesbania, representing S. exasperata, S. grandiflora, S. sesban, S. tetraptera and S. virgata at the seedling stage, and to evaluate the initial development during the first two months after planting. The trial was conducted in a greenhouse in a randomized block design, with 4 replications and 5 plants per plot. The traits recorded were: plant height (PH), from four observations at 15-day intervals, at 17, 32, 47 and 62 days after planting; length of hypocotyl (LH) and epicotyl (LEP); length (LE1) and width (WE1) of the first eophyll; and number of leaflet pairs of the second metaphyll (NLP), evaluated 17 days after planting. Univariate analyses of variance were performed, estimating the genetic parameters: coefficient of genotypic determination (b) and of genetic variation (CVg). Cluster analysis was also obtained, using the average Euclidean distance and Unweighted pair group method of arithmetic average (UPGMA) method. At 17 days after planting, S. exasperata presented the highest PH, followed by S. virgata. At 62 days after planting, S. sesban registered the highest PH. Length of hypocotyl displayed inter but not intraspecific variation. The characters LEP, LE1, WE1 and NLP showed both inter and intraspecific variation. Cluster analysis indicated the existence of 7 groups, separating the species and revealing intraspecific variation as well. The occurrence in low frequencies of two unifoliolate opposite eophylls for some species was observed, as well as bi- or trifoliolate first eophylls for one of the S. sesban accessions. These informations are basic for the selection of traits to be utilized for characterization and differentiation of Sesbania germplasm at the juvenile phase.

scholarly journals Genetic diversity of local sorghum (Sorghum bicolor) genotypes of East Java, Indonesia for agro-morphological and physiological traits

2019 ◽  
Vol 20 (9) ◽  
Author(s):  
Sulistyawati Sulistyawati ◽  
DYAH ROESWITAWATI ◽  
JABAL TARIK IBRAHIM ◽  
MAFTUCHAH
Keyword(s):  
Genetic Diversity ◽  
Sorghum Bicolor ◽  
Block Design ◽  
Physiological Traits ◽  
Breeding Programs ◽  
Pests And Diseases ◽  
Randomized Block Design ◽  
Morphological And Physiological Traits ◽  
Sorghum Genotypes ◽  
Physiological Characters

Abstract. Sulistyawati, Roeswitawati D, Ibrahim JT, Maftuchah. 2019. Genetic diversity of local sorghum (Sorghum bicolor) genotypes of East Java, Indonesia for agro-morphological and physiological traits. Biodiversitas 20: 2503-2510. Sorghum (Sorghum bicolor (L.) Moench) has great potential to be cultivated because it has extensive adaptability, tolerant to drought and puddles, can produce on marginal land and relatively resistant to pests and diseases. To meet the food requirement, sorghum can be grown in Indonesia as an alternative food source other than rice. This study aims to obtain information on the agro-morphological and physiological characters of nine local sorghum genotypes in East Java, Indonesia so that they can be used as parents in improving the nature of varieties. The experiment was conducted in a Randomized Block Design, using nine local sorghum genotypes from East Java in three replications. The nine local sorghum genotypes are Sb.Pas, Sb.Lmg 1, Sb.Lmg 2, Sb.Tbn, Sb.Spg 1, Sb.Spg 2, Sb.Tag 1, Sb.Tag 2 and Sb.Jbg. The result showed that nine genotypes that are characterized have a variety of morphological (quantitative and qualitative), agronomic and physiological characters. According to the whole characters observed, there are five genotypes that are recommended for breeding programs, namely Sb.Lmg 1, Sb.Tbn, Sb.Spg 2, Sb.Tag1 and sb.Tag2. This can be proved by the morphological character, genotype Sb.Lmg 1, Sb.Tbn, Sb.Spg 2, Sb.Tag 1 and Sb.Tag2 have a high value of Agronomy character, genotype Sb.Tbn, Sb.Tag 1 and Sb.Tag 2 noted highest production acres-1 harvest age of genjah and medium; the Physiology character, high protein and carbohydrate substances reached by the Sb.Lmg 1 genotype, Sb.Tbn and Sb.Spg 2.

scholarly journals Morphological descriptors for the characterization of teak clones (Tectona grandis L.f.) in plantations

2020 ◽  
Vol 29 (2) ◽  
pp. eRC02
Author(s):  
Jorge-L. Reategui-Betancourt ◽  
Daniele Aparecida-A. Arriel ◽  
Sidney-Fernando Caldeira ◽  
Antonio-Rioyei Higa ◽  
Paulo-C. Flôres-Junior ◽  
...  
Keyword(s):  
Block Design ◽  
Similarity Measures ◽  
Morphological Characteristics ◽  
Tectona Grandis ◽  
Group Method ◽  
Morphological Descriptors ◽  
Mato Grosso ◽  
Pair Group ◽  
Randomized Block Design ◽  
Clonal Test

Aim of study: The objective of this work was to identify possible morphological descriptors for teak clones, in order to support the cultivars protection process of this species.Area of study: This experiment was carried out in ‘São José dos Quatro Marcos’, Mato Grosso, midwest Brazil.Material and methods: A teak clonal test, assessing 18 clones, was evaluated at the ages of 29 and 41 months by means of 41 morphological characteristics, related mainly to the branches, leaves and trunk. The clonal test was established in a randomized block design composed by three blocks, each block containing 18 plots, one for each clone. Each plot had 36 plants, but only the innermost five individuals were selected and evaluated. The information was organized in a presence and absence matrix. Subsequently, genetic similarity measures were estimated, by means of the Jaccard index, and a clustering was performed by the Unweighted Pair Group Method using the Arithmetic averages (UPGMA) method.Main results: A total of 26 and 28 morphological characteristic that exhibited DHS (distinction, homogeneity and stability) were identified at the ages of 29 and 41 months, respectively. Of these, 17 characteristics showed the same behavior at 29 and 41 months of age. However, it is important to emphasize that the evaluation must be performed under the same planting conditions in which these descriptors were developed.Research highlights: These 17 morphological characteristics can compose the list of potential morphological descriptors to be used in the process of teak clones/cultivars protection.Keywords: cultivars protection; morphological characteristics; distinction, homogeneity; stability.

scholarly journals Morphological and SSR Marker Based Diversity Analysis of Lentil (Lens esculenta) Genotypes using Yield and Yield Contributing Characters

2020 ◽  
Author(s):  
M. A. Malek ◽  
M. M. Chowdhury ◽  
M. A. Haque ◽  
M. Rasel ◽  
M. R. Molla ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Market Price ◽  
Group Method ◽  
Improvement Program ◽  
Breeding Programs ◽  
Pair Group ◽  
Ssr Primers ◽  
Selection For ◽  
Set Up ◽  
Selection Of

Lentil is an important pulse crop with high nutritional value and high market price worldwide. Molecular markers have emerged as useful tools to assess the genetic diversity across crops. The study was conducted to explore genetic diversity of twenty lentil genotypes considering yield and yield attributing traits. Among all genotypes, BARI Masur-6, BARI Masur-7 followed by genotypes BD-3806 and BD-4090 showed the highest value of yield attributing traits therefore, these genotypes are considered as best performer. The results of cluster analysis based on the Ward’s method grouped the genotypes into three clusters and the genotypes of cluster III revealed the maximum value for yield per plants which indicated their importance in the selection for yield improvement program of lentil. Afterwards, 20 genotypes were evaluated through 7 sets of SSR primers to assess genetic diversity among the genotypes. Among them, four sets of primers viz., SSR 19, SSR 33, SSR 90 and SSR 213 showed high polymorphism which suggesting the greater genetic diversity in the genotypes. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei’s (1973) genetic distance led the genotypes into four major clusters which showed a bit deviation with the morphological cluster. The findings of this study will be very useful for selection of appropriate parents and the genetic understanding for the set up for future systematic lentil breeding programs.

scholarly journals Assessment of genetic diversity of different tomato genotypes using RAPD markers

2019 ◽  
Vol 29 (4) ◽  
pp. 276-283
Author(s):  
M Paul ◽  
SR Saha
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Group Method ◽  
Breeding Programs ◽  
Pair Group ◽  
Information Indices ◽  
Tomato Breeding ◽  
Tomato Genotypes

The present investigation was carried out for assessment of genetic diversity among the 28 tomato genotypes though three random amplified polymorphic DNA (RAPD) markers. A total of 15 distinct DNA fragments ranging form 100-1000 bp were amplified by using three selected primers of which 5.00 polymorphic bands per primer and over all polymorphic loci was 100 percent. The extent of genetic diversity among these genotypes was computed through parameters of genetic diversity and Shanon’s information indices. The highest genetic distance was observed among the accession Cl-3d-0-99 (V93)  vs. F1 (G X V12), F1 (G X V17), F1 (G X V29) and  Durch fuegel  (G) vs. F1 (G X V17) and F1 (G X V93) vs. F1 (G X V12), F1 (G X V17), while the lowest genetic distance was observed among the accessions Fut. Wed Abrid (V94) vs. Sunlight pole (V67) and F1 (V67 X VG) vs. F1 (V17 X VG), F1 (V93 X VG) and F1 (V93 X V17) vs. F1 (V17 X VG), F1 (V67 X VG), F1 (V93 X VG). The Unweighted Pair Group Method of Arithmetic Mean (UPGMA) dendrogram based on Nei’s genetic distance divided the genotypes into two main clusters: A & B. Cluster ‘A’ consists of 19 accessions and cluster ‘B’ consists of 9 accessions. The information generated from this study could be useful in gene mapping and marker assisted breeding for future tomato breeding programs. Progressive Agriculture 29 (4): 276-283, 2018

Sign in / Sign up

Export Citation Format

Share Document