scholarly journals R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)

1999 ◽  
Vol 22 (2) ◽  
pp. 201-204 ◽  
Author(s):  
Edson Luis Maistro ◽  
Fausto Foresti ◽  
Claudio Oliveira
Keyword(s):  
Banding Pattern ◽  
Banding Technique ◽  
Fish Genome ◽  
Banding Patterns ◽  
Technical Problems ◽  
Replication Banding ◽  
Astyanax Scabripinnis ◽  
Fish Chromosomes ◽  
Restriction Endonuclease Bamhi

The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.

Replication banding patterns in Atlantic salmon (Salmo salar)

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 440-444 ◽  
Author(s):  
A. M. Pendás ◽  
P. Morán ◽  
E. García-Vázquez
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Banding Pattern ◽  
Staining Method ◽  
Chromosome Size ◽  
Banding Patterns ◽  
Replication Banding ◽  
C Banding ◽  
Atlantic Salmon Salmo Salar

Replication banding patterns have been obtained from in vivo treatment of Salmo salar using a modification of the 5-BrdU technique and in kidney cultures using the FPG staining method. Most of the chromosome pairs were identified in the karyotype based on the banding pattern, chromosome size, and centromere position. C-banding and replication banding patterns were compared.Key words: karyotype, replication banding, Salmo salar.

scholarly journals BIOSYNTHESIS IN ISOLATED ACETABULARIA CHLOROPLASTS

1972 ◽  
Vol 54 (2) ◽  
pp. 279-294 ◽  
Author(s):  
David C. Shephard ◽  
Wendy B. Levin
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Technical Problems ◽  
Hydrolyzed Protein ◽  
Protein Amino Acids ◽  
Amino Acid Labeling ◽  
Labeling Pattern

The ability of chloroplasts isolated from Acetabulana mediterranea to synthesize the protein amino acids has been investigated. When this chloroplast isolate was presented with 14CO2 for periods of 6–8 hr, tracer was found in essentially all amino acid species of their hydrolyzed protein Phenylalanine labeling was not detected, probably due to technical problems, and hydroxyproline labeling was not tested for The incorporation of 14CO2 into the amino acids is driven by light and, as indicated by the amount of radioactivity lost during ninhydrin decarboxylation on the chromatograms, the amino acids appear to be uniformly labeled. The amino acid labeling pattern of the isolate is similar to that found in plastids labeled with 14CO2 in vivo. The chloroplast isolate did not utilize detectable amounts of externally supplied amino acids in light or, with added adenosine triphosphate (ATP), in darkness. It is concluded that these chloroplasts are a tight cytoplasmic compartment that is independent in supplying the amino acids used for its own protein synthesis. These results are discussed in terms of the role of contaminants in the observed synthesis, the "normalcy" of Acetabularia chloroplasts, the synthetic pathways for amino acids in plastids, and the implications of these observations for cell compartmentation and chloroplast autonomy.

Applications of Advanced Nanotechnology in Stem Cell Research

2021 ◽  
Vol 13 (2) ◽  
pp. 188-198
Author(s):  
Chih-Hui Yang ◽  
Shu-Ling Huang ◽  
Yi-Ting Wang ◽  
Chun-Ho Chang ◽  
Ya-Chi Tsai ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Research ◽  
Biomedical Applications ◽  
Academic Research ◽  
Review Article ◽  
Technical Problems ◽  
Biomedical Industry ◽  
Significant Attention

Nanotechnology gives rise to new breakthroughs and developments in various fields. The applications of advanced nanotechnology may resolve the current technical problems encountered in stem cell research. Nanotechnology has gained significant attention in both academic research and the biomedical industry in recent years. In this mini-review article, the progress of nanotechnology-aided stem cell studies has been surveyed, and the in vitro and in vivo applications of nanotechnology have been introduced. The in vitro studies are divided into three categories: isolation, detection, and regulation. The progress of in vivo studies and trends in biomedical applications have also been addressed.

scholarly journals Involement of an acrosinlike proteinase in the sulfhydryl-induced degradation of rabbit sperm nuclear protamine

1980 ◽  
Vol 85 (1) ◽  
pp. 116-121 ◽  
Author(s):  
BR Zirkin ◽  
TSK Chang ◽  
J Heaps
Keyword(s):  
Proteolytic Activity ◽  
Banding Pattern ◽  
Basic Protein ◽  
Polyacrylamide Gels ◽  
Banding Patterns ◽  
Pattern Characteristic ◽  
Sperm Nuclei ◽  
Triton X ◽  
Esterase Inhibitor

Previous studies demonstrated that proteolytic activity is associated with isolated rabbit sperm nuclei and is responsible for the degradation of nuclear protamine that occurs during thiol-induced in vitro decondensation of the nuclei (Zirkin and Chang, 1977; Chang and Zirkin, 1978). In this study, we present the results of experiments designed to characterize this proteolytic activity. Basic protein isolated from rabbit sperm nuclei incubated with 5 mM dithiothreitol (DTT) and 1 percent Triton X-100 for increasing periods of time exhibited progressively faster migrating bands on acid-urea polyacrylamide gels, reflection the progressive degradation of protamine. Ultimately, a specific and characteristic peptide banding pattern resulted. When sperm nuclei were treated with the esterase inhibitor nitrophenyl-p-guanidino benzoate (NPGB) to inhibit the nuclear-associated proteolytic activity and then incubated with one of several exogenous proteinases in addition to DTT and Triton X-100, characteristic peptide banding patterns were seen for each exogenous proteinase employed. For trypsin, chymotrypsin, pronase, and papain, the peptide banding patterns differed from one another and from the pattern characteristic of protamine degradation by the nuclear-associated proteinase. By contrast, when rabbit acrosin served as the exogenous proteinase, the peptide banding pattern seen was identical to the pattern characteristic of the nuclear-associated proteinase. These results demonstrate directly that the proteinase associated with rabbit sperm nuclei and involved in sperm nuclear decondensation in vitro is acrosinlike.

A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 215-219 ◽  
Author(s):  
A. Zacharopoulou ◽  
K. Bourtzis ◽  
Ph. Kerremans
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Banding Pattern ◽  
Ceratitis Capitata ◽  
Polytene Chromosomes ◽  
Biological Significance ◽  
Fruit Fly ◽  
Banding Patterns ◽  
Homologous Chromosomes ◽  
Different Tissues

The banding patterns of polytene chromosomes in different tissues of the Mediterranean fruit fly, Ceratitis capitata, vary to such an extent that homologous chromosomes cannot be recognised. However, analyses of autosomal breakpoints in several translocation strains allowed chromosomes from the two tissues to be aligned despite their difference in banding pattern. These results were discussed, considering the different hypotheses of the origin and biological significance of polytene chromosome bands.Key words: polytene chromosomes, salivary gland chromosomes, orbital bristle trichogen cell chromosomes, Ceratitis capitata.

Natural Compounds from Plant Waste for Textile Processing: Construction and Evaluation of Dyeing and Finishing with Extracting Solution of Tea-Stalk and Apocynum Halm

2015 ◽  
Vol 671 ◽  
pp. 115-120 ◽  
Author(s):  
Ji Xian Gong ◽  
Yan Fei Ren ◽  
Hui Qin Li ◽  
Zheng Li ◽  
Qiu Jin Li ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Uv Protection ◽  
Fastness Properties ◽  
Textile Processing ◽  
Protection Level ◽  
Technical Problems ◽  
Textile Dyeing ◽  
Plant Waste ◽  
And Function

Nature provides readyanswers to scientific and technical problems and inspires us with a series of technologicalinnovations. The distribution of pigment in vivo inspired theprocess of fabric finishing. Naturalcompounds from plant waste was employed for textile processing in thisinvestigation and the method of dyeing and finishing simultaneous wasconstructed. Capacity and function of the process was evaluated through colour yield and fastness, antifungalactivity and UV –protection performance. Tawny colour was obtained in theprotein fabrics processed with the extracting solution of tea-stalk and Apocynum halm. The fastness properties of both tea and Apocynum dyed samplesare quite satisfactory for practical textile dyeing purposes, especially thewool fabrics processed with tea-stalk extracting solution. And the protein fabric samples treated with tea and Apocynum waste solutionshowed good inhibitory effect (>50%) against E.coli and S.aureus. UV protection level ofprotein fabrics were increased with the treatment by extracting solution of tea-stalkand Apocynum halm.

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