The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli

This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi extract altered significantly (p<0.05) the %ATI of P, BC, IF-P, SF-P, IF-BC and SF-BC, as well as increased the survival of bacterial cultures treated with stannous chloride. Our results suggest that reishi extract could present a redox/chelating action, altering the labeling of blood constituents with 99mTc and protecting bacterial cultures against oxidative damage induced by stannous chloride.

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Evaluation of the effect of an extract of sabugueiro (Sambucus australis) on the labeling of blood constituents with technetium-99m

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600020 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 161-166

Author(s):

Camila Godinho Ribeiro ◽

Bernardo Machado Rebello ◽

Rosane de Figueiredo Neves ◽

Sebastião David Santos-Filho ◽

Adenilson de Souza da Fonseca ◽

...

Keyword(s):

Nuclear Medicine ◽

Blood Cells ◽

Trichloroacetic Acid ◽

Wistar Rats ◽

Blood Samples ◽

Chemical Substances ◽

Blood Constituents ◽

Technetium 99M

Sambucus australis (sabugueiro) has been used to treat inflammatory and rheumatologic disorders. Blood constituents labeled with technetium-99m (99mTc) have been used in nuclear medicine to obtain diagnostic images. The aim of this work was to evaluate the effect of a sabugueiro extract on the labeling of blood cells with 99mTc. Blood samples from Wistar rats were incubated with sabugueiro extract and the radiolabeling assay of blood constituents was carried out. After centrifugation, samples of plasma and blood cells were separated. Aliquots of plasma and blood cells were precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions. The radioactivity in each fraction was counted and the percentage of activity (%ATI) was determined. Incubation with sabugueiro extract altered significantly (p<0.05) the %ATI incorporated to the blood constituents. These results could be explained due the presence of chemical substances in the sabugueiro extract that present redox and/or chelating action altering the labeling of the blood constituents with 99mTc.

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Effects of Chrysobalanus icaco on the labeling of blood constituents with technetium-99m and on the shape of the red blood cells

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600018 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 145-152 ◽

Cited By ~ 3

Author(s):

Giuseppe Antonio Presta ◽

Sebastião David Santos-Filho ◽

Severo de Paoli ◽

Tania Santos Giani ◽

Adalgisa Ieda Maiworm ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Wistar Rats ◽

Area Ratio ◽

Membrane Structures ◽

Blood Samples ◽

Blood Constituents ◽

Technetium 99M ◽

Clinical Disorders ◽

Treatment Of Diabetes

Chrysobalanus icaco (abajeru; C.icaco) is recommended in the treatment of diabetes and other clinical disorders. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this study was to verify the effects of an abajeru extract on the labeling of blood constituents with 99mTc and on the shape of red blood cells (RBC). Blood samples(Wistar rats) were incubated with abajeru extract and the labeling of blood constituents with 99mTc and morphology of RBC were carried out. The results showed significant (P<0.05) alteration of labeling of blood constituents with 99mTc and the morphometry (perimeter/area ratio) of the RBC in presence of the extract. These data suggest that this abajeru extract could alter the labeling of blood constituents with 99mTc by its chelating/antioxidant action and/or effects on membrane structures involved in the ion transport.

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Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600021 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 167-174 ◽

Cited By ~ 2

Author(s):

Rosane de Figueiredo Neves ◽

Silvana Ramos Farias Moreno ◽

Bernardo Machado Rebello ◽

Luiz Querino de Araújo Caldas ◽

Adenilson de Souza da Fonseca ◽

...

Keyword(s):

Nuclear Medicine ◽

Red Blood Cells ◽

Blood Cells ◽

Wistar Rats ◽

Area Ratio ◽

Arctium Lappa ◽

Blood Samples ◽

Blood Constituents ◽

Technetium 99M ◽

Inflammatory Processes

Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock extract significantly (p<0.05) altered the %ATI on the blood compartments and the perimeter/area ratio of RBC, as well as, induced modifications on the shape of RBC. Alterations on membrane could justify the decrease of labeling of blood cells with 99mTc obtained in this study.

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Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132008000700021 ◽

2008 ◽

Vol 51 (spe) ◽

pp. 127-133 ◽

Cited By ~ 2

Author(s):

Gabrielle de Souza Rocha ◽

Marcia de Oliveira Pereira ◽

Mônica de Oliveira Benarroz ◽

Jacques Natan Grinapel Frydman ◽

Angélica Beatriz Garcia-Pinto ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Wistar Rats ◽

Quantitative Morphology ◽

Blood Samples ◽

Blood Constituents ◽

Qualitative And Quantitative ◽

Technetium 99M ◽

Sodium Pertechnetate ◽

Blood Smears

This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical microscopy. In biodistribution experiments, sodium pertechnetate was administrated, organs and tissues isolated, radioactivity was counted and percentage of incorporated radioactivity per gram (%ATI/g) determined. The data showed no significant alterations in %ATI, morphology of RBC and in %ATI/g in the studied organs.

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Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600015 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 123-128 ◽

Cited By ~ 3

Author(s):

Danielle Amorim Terra ◽

Lúcia de Fátima Amorim ◽

Maria Tereza Jansen de Almeida Catanho ◽

Adenilson de Souza da Fonseca ◽

Sebastião David Santos-Filho ◽

...

Keyword(s):

Blood Cells ◽

Plasma Proteins ◽

Trichloroacetic Acid ◽

Wistar Rats ◽

Insoluble Fraction ◽

Artemisia Vulgaris ◽

Blood Samples ◽

Blood Constituents ◽

Technetium 99M

The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p<0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble fraction). The analysis of the results indicates that the extract could have substances that could interfere on the transport of stannous through the erythrocyte membrane alterating the labeling of blood cells with 99mTc.

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Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132008000700035 ◽

2008 ◽

Vol 51 (spe) ◽

pp. 215-219 ◽

Cited By ~ 2

Author(s):

Claudia Leite Diniz ◽

Fernanda Santos do Carmo ◽

Danielle Santos de Almeida ◽

Sebastião David Santos-Filho ◽

Sotiris Missailidis ◽

...

Keyword(s):

Blood Cells ◽

Acute Treatment ◽

Wistar Rats ◽

Centella Asiatica ◽

Insoluble Fraction ◽

Blood Samples ◽

Blood Constituents ◽

Technetium 99M ◽

Sodium Pertechnetate ◽

Soluble Fractions

This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p<0.05) the %ATI/g in several organs/tissues (spleen, heart, duodenum, stomach, liver, muscle, kidney, testis and blood) and the %ATI on the blood constituents (P, BC, IF-P and IF-BC). These results indicate that the substances or metabolites of the CA extract would change the biodistribution of Na99mTcO4 and the fixation of the technetium-99m on blood constituents in an acute treatment.

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Forced Swim Alters the Radiolabeling of Blood Constituents from Wistar Rats

Applied Sciences ◽

10.3390/app10031116 ◽

2020 ◽

Vol 10 (3) ◽

pp. 1116

Author(s):

Adenilson S. da Fonseca ◽

Gabrielle S. Rocha ◽

Márcia O. Pereira ◽

André L. B. D. Cardoso ◽

Eric H. F. F. Frederico ◽

...

Keyword(s):

Blood Cells ◽

Plasma Proteins ◽

Wistar Rats ◽

Acute Stress ◽

Forced Swimming ◽

Blood Samples ◽

Blood Constituents ◽

Forced Swim ◽

99Mtc Labeling ◽

Soluble Fractions

The present study investigated the effects of forced swimming on the technetium-99m (99mTc) labeling of blood constituents (BloCs). Rats (Wistar) were submitted to forced swim. In previous experiments, swimming animals would recover for different periods of time. Animals not submitted to swimming were used as control. Blood samples were obtained and the 99mTc labeling of BloCs was carried out. Blood cells (BCs), plasma (P), insoluble fractions (IF-P and IF-BCs), and soluble fractions (SF-P and SF-BC) were isolated. Radioactivity was determined, and the percentage of 99mTc incorporated (%ATI) was calculated in each fraction. Results showed that forced swimming decreased the percentage of 99mTc incorporated (%ATI) in IF-P (p < 0.05). It is suggested that the 99mTc labeling of BloCs could be used to verify the effects of the stress conditions on BloCs and that the radionuclide fixation on plasma proteins might be altered in rats submitted to acute stress induced by forced swimming, returning to control levels after recovery.

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Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132008000700022 ◽

2008 ◽

Vol 51 (spe) ◽

pp. 135-141 ◽

Cited By ~ 2

Author(s):

Marcia de Oliveira Pereira ◽

Gabrielle de Souza Rocha ◽

Simone dos Santos Lombardi ◽

Mauro Geller ◽

Mário José Pereira ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Stannous Chloride ◽

Quantitative Morphology ◽

Blood Constituents ◽

Qualitative And Quantitative ◽

Technetium 99M ◽

Rbc Membrane ◽

Blood Smears ◽

Chloride Effect

The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel electrophoresis procedure was carried out to evaluate genotoxic and the protection of this drug against stannous chloride effect on DNA. In conclusion, under the conditions used in this work, our data suggest that fenoprofen would not (i) affect the fixation of the 99mTc on the blood constituents, (ii) alter the RBC membrane and (iii) present genotoxic and redox effects.

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Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600022 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 175-182 ◽

Cited By ~ 6

Author(s):

Severo de Paoli ◽

Tania Santos Giani ◽

Giuseppe Antonio Presta ◽

Marcia Oliveira Pereira ◽

Adenilson de Souza da Fonseca ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Cellular Membrane ◽

Area Ratio ◽

Morphometric Parameter ◽

Blood Constituents ◽

Technetium 99M ◽

Clinical Procedures ◽

Clove Extract ◽

Blood Fraction

Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p<0.05) the %ATI of blood constituents and the shape of red blood cells without modifying the perimeter/area ratio. The results indicate that clove extract presents chemical compounds that interfere with the radiolabeling of blood constituents and alter the morphology of red blood cells by oxidative/chelating actions or interacting with the cellular membrane structure.

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