scholarly journals Forced Swim Alters the Radiolabeling of Blood Constituents from Wistar Rats

2020 ◽  
Vol 10 (3) ◽  
pp. 1116
Author(s):  
Adenilson S. da Fonseca ◽  
Gabrielle S. Rocha ◽  
Márcia O. Pereira ◽  
André L. B. D. Cardoso ◽  
Eric H. F. F. Frederico ◽  
...  
Keyword(s):  
Blood Cells ◽  
Plasma Proteins ◽  
Wistar Rats ◽  
Acute Stress ◽  
Forced Swimming ◽  
Blood Samples ◽  
Blood Constituents ◽  
Forced Swim ◽  
99Mtc Labeling ◽  
Soluble Fractions

The present study investigated the effects of forced swimming on the technetium-99m (99mTc) labeling of blood constituents (BloCs). Rats (Wistar) were submitted to forced swim. In previous experiments, swimming animals would recover for different periods of time. Animals not submitted to swimming were used as control. Blood samples were obtained and the 99mTc labeling of BloCs was carried out. Blood cells (BCs), plasma (P), insoluble fractions (IF-P and IF-BCs), and soluble fractions (SF-P and SF-BC) were isolated. Radioactivity was determined, and the percentage of 99mTc incorporated (%ATI) was calculated in each fraction. Results showed that forced swimming decreased the percentage of 99mTc incorporated (%ATI) in IF-P (p < 0.05). It is suggested that the 99mTc labeling of BloCs could be used to verify the effects of the stress conditions on BloCs and that the radionuclide fixation on plasma proteins might be altered in rats submitted to acute stress induced by forced swimming, returning to control levels after recovery.

scholarly journals Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m

2007 ◽  
Vol 50 (spe) ◽  
pp. 123-128 ◽  
Author(s):  
Danielle Amorim Terra ◽  
Lúcia de Fátima Amorim ◽  
Maria Tereza Jansen de Almeida Catanho ◽  
Adenilson de Souza da Fonseca ◽  
Sebastião David Santos-Filho ◽  
...  
Keyword(s):  
Blood Cells ◽  
Plasma Proteins ◽  
Trichloroacetic Acid ◽  
Wistar Rats ◽  
Insoluble Fraction ◽  
Artemisia Vulgaris ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M

The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p<0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble fraction). The analysis of the results indicates that the extract could have substances that could interfere on the transport of stannous through the erythrocyte membrane alterating the labeling of blood cells with 99mTc.

scholarly journals Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents

2008 ◽  
Vol 51 (spe) ◽  
pp. 215-219 ◽  
Author(s):  
Claudia Leite Diniz ◽  
Fernanda Santos do Carmo ◽  
Danielle Santos de Almeida ◽  
Sebastião David Santos-Filho ◽  
Sotiris Missailidis ◽  
...  
Keyword(s):  
Blood Cells ◽  
Acute Treatment ◽  
Wistar Rats ◽  
Centella Asiatica ◽  
Insoluble Fraction ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Sodium Pertechnetate ◽  
Soluble Fractions

This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p<0.05) the %ATI/g in several organs/tissues (spleen, heart, duodenum, stomach, liver, muscle, kidney, testis and blood) and the %ATI on the blood constituents (P, BC, IF-P and IF-BC). These results indicate that the substances or metabolites of the CA extract would change the biodistribution of Na99mTcO4 and the fixation of the technetium-99m on blood constituents in an acute treatment.

scholarly journals Evaluation of the effect of an extract of sabugueiro (Sambucus australis) on the labeling of blood constituents with technetium-99m

2007 ◽  
Vol 50 (spe) ◽  
pp. 161-166
Author(s):  
Camila Godinho Ribeiro ◽  
Bernardo Machado Rebello ◽  
Rosane de Figueiredo Neves ◽  
Sebastião David Santos-Filho ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Nuclear Medicine ◽  
Blood Cells ◽  
Trichloroacetic Acid ◽  
Wistar Rats ◽  
Blood Samples ◽  
Chemical Substances ◽  
Blood Constituents ◽  
Technetium 99M

Sambucus australis (sabugueiro) has been used to treat inflammatory and rheumatologic disorders. Blood constituents labeled with technetium-99m (99mTc) have been used in nuclear medicine to obtain diagnostic images. The aim of this work was to evaluate the effect of a sabugueiro extract on the labeling of blood cells with 99mTc. Blood samples from Wistar rats were incubated with sabugueiro extract and the radiolabeling assay of blood constituents was carried out. After centrifugation, samples of plasma and blood cells were separated. Aliquots of plasma and blood cells were precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions. The radioactivity in each fraction was counted and the percentage of activity (%ATI) was determined. Incubation with sabugueiro extract altered significantly (p<0.05) the %ATI incorporated to the blood constituents. These results could be explained due the presence of chemical substances in the sabugueiro extract that present redox and/or chelating action altering the labeling of the blood constituents with 99mTc.

scholarly journals Effects of Chrysobalanus icaco on the labeling of blood constituents with technetium-99m and on the shape of the red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 145-152 ◽  
Author(s):  
Giuseppe Antonio Presta ◽  
Sebastião David Santos-Filho ◽  
Severo de Paoli ◽  
Tania Santos Giani ◽  
Adalgisa Ieda Maiworm ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Area Ratio ◽  
Membrane Structures ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Clinical Disorders ◽  
Treatment Of Diabetes

Chrysobalanus icaco (abajeru; C.icaco) is recommended in the treatment of diabetes and other clinical disorders. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this study was to verify the effects of an abajeru extract on the labeling of blood constituents with 99mTc and on the shape of red blood cells (RBC). Blood samples(Wistar rats) were incubated with abajeru extract and the labeling of blood constituents with 99mTc and morphology of RBC were carried out. The results showed significant (P<0.05) alteration of labeling of blood constituents with 99mTc and the morphometry (perimeter/area ratio) of the RBC in presence of the extract. These data suggest that this abajeru extract could alter the labeling of blood constituents with 99mTc by its chelating/antioxidant action and/or effects on membrane structures involved in the ion transport.

scholarly journals Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 167-174 ◽  
Author(s):  
Rosane de Figueiredo Neves ◽  
Silvana Ramos Farias Moreno ◽  
Bernardo Machado Rebello ◽  
Luiz Querino de Araújo Caldas ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Nuclear Medicine ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Area Ratio ◽  
Arctium Lappa ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Inflammatory Processes

Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock extract significantly (p<0.05) altered the %ATI on the blood compartments and the perimeter/area ratio of RBC, as well as, induced modifications on the shape of RBC. Alterations on membrane could justify the decrease of labeling of blood cells with 99mTc obtained in this study.

scholarly journals Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats

2008 ◽  
Vol 51 (spe) ◽  
pp. 127-133 ◽  
Author(s):  
Gabrielle de Souza Rocha ◽  
Marcia de Oliveira Pereira ◽  
Mônica de Oliveira Benarroz ◽  
Jacques Natan Grinapel Frydman ◽  
Angélica Beatriz Garcia-Pinto ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Quantitative Morphology ◽  
Blood Samples ◽  
Blood Constituents ◽  
Qualitative And Quantitative ◽  
Technetium 99M ◽  
Sodium Pertechnetate ◽  
Blood Smears

This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical microscopy. In biodistribution experiments, sodium pertechnetate was administrated, organs and tissues isolated, radioactivity was counted and percentage of incorporated radioactivity per gram (%ATI/g) determined. The data showed no significant alterations in %ATI, morphology of RBC and in %ATI/g in the studied organs.

scholarly journals An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m

2007 ◽  
Vol 50 (spe) ◽  
pp. 111-116 ◽  
Author(s):  
Tania Santos Giani ◽  
Severo de Paoli ◽  
Giuseppe Antonio Presta ◽  
Adalgisa Ieda Maiworm ◽  
Sebastião David Santos-Filho ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Cellular Membrane ◽  
Blood Constituents ◽  
Technetium 99M ◽  
99Mtc Labeling ◽  
Blood Cell And Plasma

Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated. Buzhong extract at the highest concentrations used altered significantly (p<0.05) the %ATI in blood constituents. Substances present in the Buzhong extract could alter the cellular membrane and/or generation of free radicals that have oxidant properties modifying the labeling of blood constituents with 99mTc.

scholarly journals The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli

2008 ◽  
Vol 51 (spe) ◽  
pp. 157-162 ◽  
Author(s):  
Raquel Terra Agostinho ◽  
Sebastião David Santos-Filho ◽  
Adenilson de Souza da Fonseca ◽  
Sotiris Missailidis ◽  
Mario Bernardo-Filho
Keyword(s):  
Escherichia Coli ◽  
Blood Cells ◽  
Ganoderma Lucidum ◽  
Wistar Rats ◽  
Stannous Chloride ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Bacterial Cultures

This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi extract altered significantly (p<0.05) the %ATI of P, BC, IF-P, SF-P, IF-BC and SF-BC, as well as increased the survival of bacterial cultures treated with stannous chloride. Our results suggest that reishi extract could present a redox/chelating action, altering the labeling of blood constituents with 99mTc and protecting bacterial cultures against oxidative damage induced by stannous chloride.

scholarly journals Reliable Measurements of theβ-Amyloid Pool in Blood Could Help in the Early Diagnosis of AD

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Pedro Pesini ◽  
Virginia Pérez-Grijalba ◽  
Inmaculada Monleón ◽  
Mercè Boada ◽  
Lluís Tárraga ◽  
...  
Keyword(s):  
Cognitive Impairment ◽  
Mild Cognitive Impairment ◽  
Early Diagnosis ◽  
Small Proportion ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Blood Samples ◽  
Striking Result ◽  
Healthy Control ◽  
Amnesic Mild Cognitive Impairment

The present study was aimed at assessing the capability of Aβ1-40 and Aβ1-42 levels in undiluted plasma (UP), diluted plasma (DP), and cell bound (CB) to distinguish between early stages of Alzheimer's disease (AD), amnesic mild cognitive impairment (MCI), and healthy control (HC). Four blood samples from each participant were collected during one month and the levels of Aβ1-40 and Aβ1-42 were determined by a blinded proprietary ELISA sandwich (Araclon Biotech. Zaragoza, Spain). First striking result was that the amount of Aβ1-40 and Aβ1-42 in UP represented only a small proportion (~15%) of the total beta-amyloid pool in blood (βAPB) described here as the sum of Aβ1-40 and Aβ1-42 in blood where they are free in plasma, bound to plasma proteins, and bound to blood cells. Furthermore, we found that levels of Aβ1-40 and Aβ1-42 in UP, DP, and CB were significantly higher in MCI when compared to HC. On average, the totalβAPB was 1.8 times higher in MCI than in HC (P=0.03) and allowed to discriminate between MCI and HC with a sensitivity and specificity over 80%. Thus, quantification of several markers of theβAPB could be useful and reliable in the discrimination between MCI and HC.

scholarly journals Effects of Cinnamomum zeylanicum treatment on radiolabeling of blood bonstituents and morphology of red blood cells in Wistar rats

2008 ◽  
Vol 51 (spe) ◽  
pp. 143-149 ◽  
Author(s):  
Mônica Oliveira Benarroz ◽  
Gabrielle de Souza Rocha ◽  
Márcia Oliveira Pereira ◽  
Mauro Geller ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Membrane Structures ◽  
Blood Constituents ◽  
Cinnamon Extract ◽  
Blood Smears ◽  
Transport Of Ions ◽  
Different Doses

The aim of this study was to evaluate the effect of in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. Animals were treated with cinnamon extract at different doses and for different periods of time. As controls, animals treated with 0.9% NaCl. Labeling of blood constituents with 99mTc was performed. Plasma, blood cells and insoluble fractions were isolated. Radioactivity in each fraction was counted and the percentage of radioactivity (%ATI) was calculated. Also, blood smears were prepared to morphological analysis of red blood cells from. Data showed that in vivo cinnamon extract did not significantly (p>0.05) modify the %ATI of blood constituents and morphology of red blood cells. The results suggest that in vivo aqueous cinnamon could not affect the membrane structures involved in transport of ions or the oxidation state of stannous and pertechnetate ions.

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