scholarly journals Genetic variability among cashew hybrids and prediction of superior combinations based on agronomic performance

2019 ◽  
Vol 54 ◽  
Author(s):  
Maraisa Crestani Hawerroth ◽  
Patricia do Nascimento Bordallo ◽  
Luis Cláudio Pessoa Oliveira ◽  
Egnesio Holanda Vale ◽  
Francisco das Chagas Vidal Neto ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Molecular Level ◽  
Issr Markers ◽  
Phenotypic Characterization ◽  
Agronomic Performance ◽  
Cashew Nut ◽  
Issr Molecular Markers ◽  
Rapd And Issr

Abstract: The objective of this work was to evaluate the use of RAPD and ISSR molecular markers to determine the genetic variability among cashew (Anacardium spp.) genotypes, and to indicate possible promising crosses based on cashew genetic variability and phenotypic performance. Ten hybrids - derived from the crosses CCP 76 x BGC 589, CCP 76 x BRS 226, CCP 76 x HAC 276-1, CCP 76 x Embrapa 51, CCP 76 x BRS 253, CCP 76 x HAC222-4, and BRS226 x Embrapa 51 - and their parents were assessed at the molecular level. The hybrids were evaluated for nut yield, mean nut weight, bored nuts, and powdery mildew on nuts (scale 0-4). The RAPD and ISSR markers were efficient in the determinaton of the genetic variability among cashew genotypes, allowing of the grouping of 21 clusters. Associated with the phenotypic characterization of cashew nut for yield, weight, and health, the used markers can efficiently identify possible combinations with higher genetic variability and higher probability of developing transgressive genotypes in segregating populations.

scholarly journals Identification and Genetic Diversity Analysis of Edible and Medicinal Malva Species Using Flow Cytometry and ISSR Molecular Markers

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 650
Author(s):  
Iwona Jedrzejczyk ◽  
Monika Rewers
Keyword(s):  
Flow Cytometry ◽  
Molecular Markers ◽  
Genome Size ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Size Estimation ◽  
Flow Cytometric ◽  
First Choice ◽  
Issr Molecular Markers

The Malva genus contains species that reveal therapeutic properties and are mostly important in medicine and the functional food industry. Its breeding, cultivation, and utilization are based on proper germplasm/plant identification, which is difficult using morphological features. For this reason, we applied flow cytometry and inter simple sequence repeat polymerase chain reaction (ISSR-PCR) for fast and accurate species identification. Genome size estimation by flow cytometry was proposed as the first-choice method for quick accession screening. Out of the 12 tested accessions, it was possible to identify six genotypes based on genome size estimation, whereas all species and varieties were identified using ISSR markers. Flow cytometric analyses revealed that Malva species possessed very small (1.45–2.77 pg/2C), small (2.81–3.80 pg/2C), and intermediate (11.06 pg/2C) genomes, but the majority of accessions possessed very small genomes. Additionally, this is the first report on genome size assessment for eight of the accessions. The relationships between the investigated accessions showed the presence of two clusters representing malvoid and lavateroid group of species. Flow cytometry and ISSR molecular markers can be effectively used in the identification and genetic characterization of Malva species.

scholarly journals RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification

2006 ◽  
Vol 54 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Paula Martins-Lopes ◽  
José Lima-Brito ◽  
Sónia Gomes ◽  
Julieta Meirinhos ◽  
Luís Santos ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Olea Europaea ◽  
Cultivar Identification ◽  
Olea Europaea L ◽  
Issr Molecular Markers ◽  
Rapd And Issr ◽  
Olea Europaéa

scholarly journals Genetic divergence in basil cultivars and hybrids

2019 ◽  
Vol 37 (2) ◽  
pp. 180-187
Author(s):  
Rodrigo P Alves ◽  
Ana Veruska C Silva ◽  
Camila S Almeida-Pereira ◽  
Tatiana S Costa ◽  
Sheila V Alvares-Carvalho ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Variability ◽  
Polymorphic Information Content ◽  
Dna Amplification ◽  
Issr Markers ◽  
Economic Importance ◽  
Dissimilarity Index ◽  
The World ◽  
Issr Molecular Markers

ABSTRACT Basil is an aromatic herb that stands out for its economic importance. It is consumed in natura and used to obtain essential oil. The cultivation of this species in several regions of the world has allowed variations by natural crosses and euploidy, leading to the wide genetic variability found nowadays. Considering the importance of this species, we aimed to analyze the genetic diversity of 27 basil genotypes using ISSR molecular markers. Fourteen primers were employed for DNA amplification, resulting in 86% polymorphism. Based on the Jaccard’s dissimilarity index, the highest index (0.80) was observed between the individuals BAS001 and BAS012, while the lowest index (0.18) was detected between the genotypes BAS014 and BAS015. The genetic similarity among individuals was calculated, forming four distinct clusters. Most individuals (40.7%) were allocated in cluster I. The polymorphic information content (PIC) (0.89) indicated considerable levels of genetic diversity among genotypes. In this sense, the ISSR markers were efficient in the detection of polymorphisms between the accessions, suggesting the genetic variability of the collection. This result demonstrates the importance of the use of molecular markers and the advantages that this information provides to the breeding of the species.

Characterization of Jordanian olive cultivars (Olea europaea L.) using RAPD and ISSR molecular markers

2014 ◽  
Vol 176 ◽  
pp. 282-289 ◽  
Author(s):  
Mohammad Brake ◽  
Hussein Migdadi ◽  
Moath Al-Gharaibeh ◽  
Salam Ayoub ◽  
Nizar Haddad ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Olea Europaea ◽  
Olea Europaea L ◽  
Olive Cultivars ◽  
Issr Molecular Markers ◽  
Rapd And Issr ◽  
Olea Europaéa

Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

2021 ◽  
Author(s):  
Warepam Amuchou Singh ◽  
Nandeibam Samarjit Singh ◽  
Elangbam Julia Devi ◽  
Pratap Jyoti Handique ◽  
Huidrom Sunitibala Devi
Keyword(s):  
Issr Markers ◽  
Gene Pools ◽  
Musa Spp ◽  
Pcr Rflp ◽  
Rapd And Issr

scholarly journals Characterization of genetic structure of alfalfa (Medicago sp.) from trans-Himalaya using RAPD and ISSR markers

2011 ◽  
Vol 10 (42) ◽  
pp. 8176-8187 ◽  
Author(s):  
Raj Xavier Janifer ◽  
Kumar Jitendra ◽  
Bihari Srivastava Ravi
Keyword(s):  
Genetic Structure ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

2017 ◽  
Vol 45 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Spyridon KINTZIOS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Root Length ◽  
In Vitro Propagation ◽  
Genetic Relationships ◽  
Shoot Proliferation ◽  
Poncirus Trifoliata ◽  
Naphthaleneacetic Acid ◽  
Issr Molecular Markers

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

Molecular characterization of jasmine genotypes using RAPD and ISSR markers

2020 ◽  
Vol 77 (1) ◽  
pp. 149
Author(s):  
Sanchita Ghosh ◽  
M. Ganga ◽  
K. Soorianathasundaram ◽  
Ajit Kumar
Keyword(s):  
Molecular Characterization ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals Morphological and Molecular Characterization of Selected Chilean Runner Bean (Phaseolus coccineus L.) Genotypes Shows Moderate Agronomic and Genetic Variability

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1688
Author(s):  
Osvin Arriagada ◽  
Andrés R. Schwember ◽  
María Jesús Greve ◽  
Milan O. Urban ◽  
Ricardo A. Cabeza ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Agronomic Traits ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Small Scale ◽  
Central Chile ◽  
Runner Bean ◽  
Agronomic Potential ◽  
Phaseolus Species

The runner bean is the third most economically important Phaseolus species, which is cultivated on small-scale agriculture for the production of immature pods or to obtain dry seeds. However, despite the economic importance and agronomic potential of this species, the runner bean has been little studied from the genetic standpoint. Therefore, the main objective of this study was to characterize ten selected lines of runner bean obtained from Central (Santiago) and Southern (Valdivia and Villarica) Chile based on morphological and agronomic traits. In addition, the genetic variability of these lines was determined using 12 Inter-Simple Sequence Repeat (ISSR) markers to evaluate the potential of this germplasm for breeding and commercial purposes. As a result, the lines from Central Chile were characterized, and had a higher number of pods per plant compared to the Southern lines, although the size and weight of their seeds were lower. Moreover, a low level of genetic diversity (He = 0.251) was encountered in this population. Finally, this is one of the first studies that generate relevant and novel information on the morphological, agronomic and genetic characterization of the P. coccineus germplasm present in Chile.

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