scholarly journals Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes

2006 ◽  
Vol 14 (5) ◽  
pp. 355-364 ◽  
Author(s):  
Janir Alves Soares ◽  
Mário Roberto Leonardo ◽  
Léa Assed Bezerra da Silva ◽  
Mário Tanomaru Filho ◽  
Izabel Yoko Ito

OBJECTIVE: The purpose of this study was to evaluate the distribution of microorganisms in the root canal system (RCS) and periapical lesions of dogs' teeth after rotary instrumentation and placement of different calcium hydroxide [Ca(OH)2]-based intracanal dressings. MATERIALS AND METHODS: Chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. Instrumentation was undertaken using the ProFile rotary system and irrigation with 5.25% sodium hypochlorite. The following Ca(OH)2-based pastes were applied for 21 days: group 1 - Calen (n=18); group 2 - Calen+CPMC (n=20); group 3 - Ca(OH)2 p.a. + anaesthetic solution (n=16) and group 4 - Ca(OH)2 p.a.+ 2% chlorhexidine digluconate (n=18). Eight root canals without endodontic treatment constituted the control group. Histological sections were obtained and stained with Brown & Brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. The results were analyzed statistically by the Mann-Whitney U test (p<0.05). RESULTS: The control group showed the highest prevalence of microorganisms in all sites evaluated. Gram-positive cocci, bacilli and filaments were the most frequent morphotypes. Similar microbial distribution patterns in the RCS and areas of cementum resorption were observed in all groups (p>0.05). The percentage of RCS sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively. CONCLUSION: In conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the RCS and periapical lesions. However, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calcium hydroxide-based intracanal dressing.

2014 ◽  
Vol 8 (3) ◽  
pp. 172-175 ◽  
Author(s):  
A Gupta ◽  
V Goyal ◽  
S Bala ◽  
J Duhan ◽  
S Hans

ABSTRACT The success of root canal treatment is based on total elimination of root canal content, thorough cleaning, shaping and obturation of root canal system. Calcium hydroxide is recommended as intra-canal medicament because of its antibacterial properties, tissue dissolving ability, inhibition of tooth resorption and indication of tissue repair by hard tissue formation. The present case series highlights the use of calcium hydroxide as an inter appointment endodontic dressing for management of large periapical radiolucency and periodically reviewed. Thus the non surgical healing of large periapical lesions provided favourable clinical and radiographic response.


2006 ◽  
Vol 20 (2) ◽  
pp. 120-126 ◽  
Author(s):  
Janir Alves Soares ◽  
Mario Roberto Leonardo ◽  
Léa Assed Bezerra da Silva ◽  
Mario Tanomaru Filho ◽  
Izabel Yoko Ito

This study aimed at evaluating the antisepsis of the root canal system (RCS) and periapical region (PR) provided by rotary instrumentation associated with chlorhexidine + calcium hydroxide as intracanal medicament. Chronic periapical lesions were induced in 26 pre-molar roots in two dogs. After microbiological sampling, automatic instrumentation using the Profile system and irrigation with 5.25% sodium hypochlorite solution, with a final rinse of 14.3% EDTA followed by profuse irrigation with physiological saline were carried out in 18 root canals. After drying the canals, a paste based on calcium hydroxide associated with a 2% chlorhexidine digluconate solution was placed inside them. After 21 days, the medication was removed, leaving the root canals empty and coronally sealed. After 96 hours, a final microbiological sample was obtained, followed by histomicrobiological processing by the Brown & Brenn method. Eight untreated root canals represented the control group (C-G). Based on the Mann-Whitney test at a confidence level of 5% (p < 0.05), the procedures of antisepsis used offered significant efficacy (p < 0.05) resulting in 100.0% of the canals free of microorganisms. In the C-G, an elevated incidence of various microbial morphotypes was confirmed in all sites of the RCS, with the presence of microbial colonies in the periapical region. In contrast, the experimental group showed a similar pattern of infection in the RCS, although less intense and a reduced level of periapical infection (p < 0.05). It was concluded that adequate instrumentation followed by the application of calcium hydroxide + chlorhexidine offered significant elimination of microorganisms.


2020 ◽  
Vol 8 (2) ◽  
pp. 90-100
Author(s):  
Ratu Amelia ◽  
Deli M

The main goal of endodontic treatment is to remove the entire microbial flora from the root canal system and promote periapical healing. Microorganisms that settle in the root system play an important role in the development and persistence of periapical lesions. Necrotic canals provide a very favorable environment for the growth of microorganisms in the root canal system. Thus, elimination of these bacteria is essential for apical and periapical healing after endodontic treatment. This case report discusses the endodontic treatment using calcium hydroxide in a necrotic pulp with chronic periapical abscess.


2011 ◽  
Vol 12 (5) ◽  
pp. 368-371 ◽  
Author(s):  
Matheus Melo Pithon ◽  
Deyla Duarte Vilela ◽  
Manoel Matos Neto ◽  
Alexandre Mascarenhas Villela

ABSTRACT Aim To evaluate the interference of the intracanal medication Calen® (SSWhite, São Paulo, Brazil) on the filling of simulated lateral canals. Materials and methods Twenty human anterior teeth were used. Before the endodontic filling procedures the access of cavity was made, and after this root canals were made in all the teeth to simulate the presence of lateral canals. After preparation, the teeth were randomly divided into two groups (n=10). In group I, the root canal system was filled directly after chemicalmechanical preparation; in group II, endodontic treatment was performed in multiple sessions, and after preparation the calcium hydroxide-based intracanal medication Calen® was inserted. After the period of 7 days, the root canals were vigorously irrigated and then they were filled. Next, the teeth were radiographed to verify the quality of the filling. Results The results demonstrated that the teeth treated in a single session, without calcium hydroxide medication, presented 47 canals out of 60 with radiographic evidence of filling, whereas the teeth in which intracanal medication was used, only 07 presented a radiographic image compatible with filling (p < 0.05). Conclusion The use of the calcium hydroxide-based medication Calen made it difficult to obtain a hermetic filling of the root canal system. Clinical significance The clinical significance of this work basing on the fact that once the dentist knowing that property obliteration of calcium hydroxide can be taken care when they are used in the presence of lateral canals. How to cite this article Vilela DD, Neto MM, Villela AM, Pithon MM. Evaluation of Interference of Calcium Hydroxide-based Intracanal Medication in Filling Root Canal Systems . J Contemp Dent Pract 2011;12(5):368-371.


2011 ◽  
Vol 139 (9-10) ◽  
pp. 579-582 ◽  
Author(s):  
Djurica Grga ◽  
Bojan Dzeletovic ◽  
Vesna Miletic ◽  
Marija Damjanov

Introduction. The function of root canal fillings is to seal the root canal system. The important physical property necessary for sealers to achieve this is low solubility. However, any therapeutic effect of calcium hydroxide-based sealers is dependent on the calcium hydroxide being in ionized form, which implies that the material must be at least partly soluble. Objective. The objective of our study was to compare weight changes of Acroseal and Apexit, conventional calcium hydroxide-based sealer and AH Plus, epoxy-amine resin sealer in Hank?s solution at different exposure times. Methods. The standardized samples of each material were weighed and immersed in the Hank?s solution for 1 h, 24 h, 96 h, 14 days and 28 days. After these exposure times, they were removed, dried, and weighed again. Mean weight changes were determined and the differences between sealers were analysed statistically using a one-way ANOVA. Results. The highest differences in mass were observed in Apexit, 1.52%, and were significantly different from Acroseal, 0.93% (p<0.05) and AH Plus, 0.45% (p<0.05). There were no significant differences between Acroseal and AH Plus, except for 96 h period. Conclusion. Under the conditions of our study, it may be concluded that the Acroseal sealer presented the behaviour more like epoxy-based material, AH Plus, than calcium hydroxide sealer, Apexit.


2021 ◽  
Vol 90 (2) ◽  
pp. e499
Author(s):  
Agata Koprowicz ◽  
Piotr Koprowicz

Introduction. Successful endodontic treatment is affected by a number of factors associated with the disinfection and filling of the root canal. The chemical-mechanical root canal preparation consists in a thorough removal of any content from the pulp space, including inflamed pulp, bacteria, as well as canal filling materials. Aim. The aim of the study was to analyse the efficacy of the XP-endo Finisher and the Brush-Finisher on the removal of a calcium hydroxide dressing. Material and Methods. The study was conducted using extracted single-rooted human teeth prepared according to sample standardization. Calcium hydroxide with iodoform was inserted into the canals. After two weeks, canal cleaning was performed with the use of 2% sodium hypochlorite solution and both finisher files. A conventional endodontic needle and syringe (SNI) were used in the control group. Following rinsing activation, two projection radiographs were performed and uploaded to software developed specifically for the study. The graphic files were evaluated in terms of the remaining amount of dressing. In order to analyse whether the percentage of the canal area that remained untreated was statistically significant, the Kruskal-Wallis ANOVA test with Dunn’s post-hoc test were employed. Results. The intracanal dressing was most effectively removed in the XP-endo Finisher group (in both projections 96.32% and 91.35%), and its removal was considerably better than that in the control group (p<0.0001), although not significantly different from the Brush-Finisher group (89.68% and 81.85%). Conclusions. Supplementary irrigant activation with either the XP-endo Finisher or the Brush-Finisher improved the removal of calcium hydroxide from the root canal walls.


2015 ◽  
Vol 3 (45) ◽  
pp. 8884-8891 ◽  
Author(s):  
Xiaoman Li ◽  
Bing Han ◽  
Xiaoyan Wang ◽  
Xuejun Gao ◽  
Fuxin Liang ◽  
...  

The treatment of apical periodontitis (AP) remains challenging because traditional root canal therapy (RCT) outcomes are limited by the complexity of the root canal system, drug toxicity, and host immune factors.


2012 ◽  
Vol 9 (2) ◽  
pp. 1-4
Author(s):  
Ibrahim Khalil ◽  
KM Mohidul Islam ◽  
Md Zahid Hossain ◽  
Arup Kumar Shah ◽  
Akashlynn Badruddoza ◽  
...  

Sterilization of bacteria in root canal system is one of the prominent problems. Some bacteria could remain in root canal even after using conventional medicaments. Evidences suggested that Enterococcus faecalis (E. faecalis) caused substantial root canal infections. So, elimination of such organism is important to achieve treatment success. Research hypothesis: Sterilization of root canal with Lesion Sterilization and Tissue Repair (LSTR)-3mix MP Therapy is thought to be more effective against E. faecalis comparing to single antibiotic. Methods: In-vitro cross-sectional with interventional type of study to observe the zone of inhibitions by E. faecalis culture using LSTR- 3mix (metronidazole, ciprofloxacin and minocycline) and calcium hydroxide (control). Results: The results strongly recommended that LSTR-3mix sufficiently able to inhibit E. faecalis growth. DOI: http://dx.doi.org/10.3329/cdcj.v9i2.12311 City Dental College J. Volume-9, Number-2, July-2012


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