scholarly journals Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs

2006 ◽  
Vol 20 (2) ◽  
pp. 120-126 ◽  
Author(s):  
Janir Alves Soares ◽  
Mario Roberto Leonardo ◽  
Léa Assed Bezerra da Silva ◽  
Mario Tanomaru Filho ◽  
Izabel Yoko Ito

This study aimed at evaluating the antisepsis of the root canal system (RCS) and periapical region (PR) provided by rotary instrumentation associated with chlorhexidine + calcium hydroxide as intracanal medicament. Chronic periapical lesions were induced in 26 pre-molar roots in two dogs. After microbiological sampling, automatic instrumentation using the Profile system and irrigation with 5.25% sodium hypochlorite solution, with a final rinse of 14.3% EDTA followed by profuse irrigation with physiological saline were carried out in 18 root canals. After drying the canals, a paste based on calcium hydroxide associated with a 2% chlorhexidine digluconate solution was placed inside them. After 21 days, the medication was removed, leaving the root canals empty and coronally sealed. After 96 hours, a final microbiological sample was obtained, followed by histomicrobiological processing by the Brown & Brenn method. Eight untreated root canals represented the control group (C-G). Based on the Mann-Whitney test at a confidence level of 5% (p < 0.05), the procedures of antisepsis used offered significant efficacy (p < 0.05) resulting in 100.0% of the canals free of microorganisms. In the C-G, an elevated incidence of various microbial morphotypes was confirmed in all sites of the RCS, with the presence of microbial colonies in the periapical region. In contrast, the experimental group showed a similar pattern of infection in the RCS, although less intense and a reduced level of periapical infection (p < 0.05). It was concluded that adequate instrumentation followed by the application of calcium hydroxide + chlorhexidine offered significant elimination of microorganisms.

2021 ◽  
Vol 27 (2) ◽  
pp. 3712-3717
Author(s):  
Tzvetelina Gueorgieva ◽  
◽  
Raina Gergova ◽  

Introduction: The purpose of endodontic treatment is to eliminate the bacterial infection in the root canal system and allow healing of apical periodontitis. Sometimes the anatomical complexity of the root canal system makes complete removal of bacteria almost impossible even if the conventional methods of chemo-mechanical debridement are performed strictly according to the protocol. So additional methods of root canal disinfection can be applied such as photoactivated disinfection (PAD). Purpose: The aim of our study was to compare the antibacterial activity of PAD with FotoSan, and conventional endodontic therapy in the treatment of infected root canals. Materials and methods: The study involved 36 teeth of patients who are diagnosed with pulp necrosis or with the periapical chronic periodontitis and require endodontic treatment and divided into two groups of 18 teeth each. The first microbiological sample was taken by placing a sterile paper point in the root canal, after endodontic access cavity preparation. All root canals are prepared by Protaper Universal rotary instruments (Maillefer Instruments SA, Ballaigues, Switzerland). In the first group, the disinfection of the root canals was made with photoactivated disinfection with FotoSan. In the second group was performed the endodontic treatment with the standard protocol of irrigation - 2.5% sodium hypochlorite solution and 17% EDTA. After that, the root canals are dried with sterile points, and a second microbiological sample is taken. Results: In all compared pairs, there was no significantly different effect regarding the number of microorganisms. Conclusions; The disinfection with NaOCl has the strongest antimicrobial effect in this study. Second place is occupied by PAD with FotoSan. We believe that the established antimicrobial effect of PAD make the method appropriate as complementary to routine endodontic treatment.


2011 ◽  
Vol 12 (5) ◽  
pp. 368-371 ◽  
Author(s):  
Matheus Melo Pithon ◽  
Deyla Duarte Vilela ◽  
Manoel Matos Neto ◽  
Alexandre Mascarenhas Villela

ABSTRACT Aim To evaluate the interference of the intracanal medication Calen® (SSWhite, São Paulo, Brazil) on the filling of simulated lateral canals. Materials and methods Twenty human anterior teeth were used. Before the endodontic filling procedures the access of cavity was made, and after this root canals were made in all the teeth to simulate the presence of lateral canals. After preparation, the teeth were randomly divided into two groups (n=10). In group I, the root canal system was filled directly after chemicalmechanical preparation; in group II, endodontic treatment was performed in multiple sessions, and after preparation the calcium hydroxide-based intracanal medication Calen® was inserted. After the period of 7 days, the root canals were vigorously irrigated and then they were filled. Next, the teeth were radiographed to verify the quality of the filling. Results The results demonstrated that the teeth treated in a single session, without calcium hydroxide medication, presented 47 canals out of 60 with radiographic evidence of filling, whereas the teeth in which intracanal medication was used, only 07 presented a radiographic image compatible with filling (p < 0.05). Conclusion The use of the calcium hydroxide-based medication Calen made it difficult to obtain a hermetic filling of the root canal system. Clinical significance The clinical significance of this work basing on the fact that once the dentist knowing that property obliteration of calcium hydroxide can be taken care when they are used in the presence of lateral canals. How to cite this article Vilela DD, Neto MM, Villela AM, Pithon MM. Evaluation of Interference of Calcium Hydroxide-based Intracanal Medication in Filling Root Canal Systems . J Contemp Dent Pract 2011;12(5):368-371.


2021 ◽  
Vol 90 (2) ◽  
pp. e499
Author(s):  
Agata Koprowicz ◽  
Piotr Koprowicz

Introduction. Successful endodontic treatment is affected by a number of factors associated with the disinfection and filling of the root canal. The chemical-mechanical root canal preparation consists in a thorough removal of any content from the pulp space, including inflamed pulp, bacteria, as well as canal filling materials. Aim. The aim of the study was to analyse the efficacy of the XP-endo Finisher and the Brush-Finisher on the removal of a calcium hydroxide dressing. Material and Methods. The study was conducted using extracted single-rooted human teeth prepared according to sample standardization. Calcium hydroxide with iodoform was inserted into the canals. After two weeks, canal cleaning was performed with the use of 2% sodium hypochlorite solution and both finisher files. A conventional endodontic needle and syringe (SNI) were used in the control group. Following rinsing activation, two projection radiographs were performed and uploaded to software developed specifically for the study. The graphic files were evaluated in terms of the remaining amount of dressing. In order to analyse whether the percentage of the canal area that remained untreated was statistically significant, the Kruskal-Wallis ANOVA test with Dunn’s post-hoc test were employed. Results. The intracanal dressing was most effectively removed in the XP-endo Finisher group (in both projections 96.32% and 91.35%), and its removal was considerably better than that in the control group (p<0.0001), although not significantly different from the Brush-Finisher group (89.68% and 81.85%). Conclusions. Supplementary irrigant activation with either the XP-endo Finisher or the Brush-Finisher improved the removal of calcium hydroxide from the root canal walls.


2005 ◽  
Vol 13 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Janir Alves Soares ◽  
Mario Roberto Leonardo ◽  
Léa Assed Bezerra da Silva ◽  
Mário Tanomaru Filho ◽  
Izabel Yoko Ito

In the endodontic treatment of root canals with necrotic pulps associated with periapical radiolucent areas, one of the main objectives of treatment consists in eliminating the microorganisms spread throughout the ramifications of the root canal system. The scope of this study was to evaluate the antiseptic efficacy of biomechanical preparation and two calcium hydroxide-based pastes, in dogs' teeth with experimentally induced chronic periapical lesions. After initial microbiological sampling, instrumentation of the root canals was undertaken using the conventional technique, using K type files used in conjunction with a solution of 5.25% sodium hypochlorite. After ninety-six hours, further microbiological sampling was undertaken and Calen/CPMC or Calasept pastes were applied for 15 and 30 days. Ninety six hours after the removal of the medication, the third microbiological sampling was undertaken and finally histomicrobiological analysis followed using Brown & Brenn staining. The results were analyzed using the Kruskall-Wallis test, with a level of significance established at 5% (p<0.05). It was shown that the biomechanical preparation significantly reduced the number of colony forming units of microorganisms in root canals. In contrast, there was no significant difference between the antiseptic action of long-term dressings over two periods of action, although 30 days produced a greater reduction in the quantity of microorganisms and of positive microbiological cultures. In histological sections of the four experimental groups, similar microbiological patterns were shown (p>0.05), characterized by an elevated incidence of cocci, bacilli and filaments, predominantly gram-positive, in root canals, secondary canals and accessories, apical cementoplasts and dentinal tubules, but with a low incidence of microorganisms in areas of cementum resorption and the periapical lesion. The biomechanical preparation and intracanal dressing based on calcium hydroxide were important in the antisepsis of the root canal; however, both procedures did not produce significant changes in the microbiological aspects of the root canal system.


2006 ◽  
Vol 14 (5) ◽  
pp. 355-364 ◽  
Author(s):  
Janir Alves Soares ◽  
Mário Roberto Leonardo ◽  
Léa Assed Bezerra da Silva ◽  
Mário Tanomaru Filho ◽  
Izabel Yoko Ito

OBJECTIVE: The purpose of this study was to evaluate the distribution of microorganisms in the root canal system (RCS) and periapical lesions of dogs' teeth after rotary instrumentation and placement of different calcium hydroxide [Ca(OH)2]-based intracanal dressings. MATERIALS AND METHODS: Chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. Instrumentation was undertaken using the ProFile rotary system and irrigation with 5.25% sodium hypochlorite. The following Ca(OH)2-based pastes were applied for 21 days: group 1 - Calen (n=18); group 2 - Calen+CPMC (n=20); group 3 - Ca(OH)2 p.a. + anaesthetic solution (n=16) and group 4 - Ca(OH)2 p.a.+ 2% chlorhexidine digluconate (n=18). Eight root canals without endodontic treatment constituted the control group. Histological sections were obtained and stained with Brown & Brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. The results were analyzed statistically by the Mann-Whitney U test (p<0.05). RESULTS: The control group showed the highest prevalence of microorganisms in all sites evaluated. Gram-positive cocci, bacilli and filaments were the most frequent morphotypes. Similar microbial distribution patterns in the RCS and areas of cementum resorption were observed in all groups (p>0.05). The percentage of RCS sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively. CONCLUSION: In conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the RCS and periapical lesions. However, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calcium hydroxide-based intracanal dressing.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alfredo Sierra-Cristancho ◽  
Luis González-Osuna ◽  
Daniela Poblete ◽  
Emilio A. Cafferata ◽  
Paola Carvajal ◽  
...  

AbstractThis study aimed to analyze the root anatomy and root canal system morphology of mandibular first premolars in a Chilean population. 186 teeth were scanned using micro-computed tomography and reconstructed three-dimensionally. The root canal system morphology was classified using both Vertucci’s and Ahmed’s criteria. The radicular grooves were categorized using the ASUDAS system, and the presence of Tomes’ anomalous root was associated with Ahmed’s score. A single root canal was identified in 65.05% of teeth, being configuration type I according to Vertucci’s criteria and code 1MP1 according to Ahmed’s criteria. Radicular grooves were observed in 39.25% of teeth. The ASUDAS scores for radicular grooves were 60.75%, 13.98%, 12.36%, 10.22%, 2.15%, and 0.54%, from grade 0 to grade 5, respectively. The presence of Tomes’ anomalous root was identified only in teeth with multiple root canals, and it was more frequently associated with code 1MP1–2 of Ahmed’s criteria. The root canal system morphology of mandibular first premolars showed a wide range of anatomical variations in the Chilean population. Teeth with multiple root canals had a higher incidence of radicular grooves, which were closely related to more complex internal anatomy. Only teeth with multiple root canals presented Tomes’ anomalous root.


2015 ◽  
Vol 3 (3) ◽  
pp. 668
Author(s):  
Fernanda Hoffmann Busanello ◽  
Angela Longo Do Nascimento ◽  
Lilian Tietz ◽  
Mirela Sangoi Barreto ◽  
Ricardo Abreu Da Rosa ◽  
...  

AIM: The aim of this study was to evaluate the efficacy of different lengths of time of passive ultrasonic irrigation (PUI) in removing calcium hydroxide (CH) paste from root canal, using scanning electron microscopy and energy dispersive spectrometry (SEM/EDS). MATERIAL AND METHODS: Fifty-four human premolars were used. After coronal access, a size #15 K-file was used to obtain apical patency. Root canal preparation was performed using WaveOne 40.08 instruments. CH paste was placed into the root canal. Specimens were stored in a humid environment, and after seven days, they were divided into five groups (n=12) according to the irrigation protocols: Manual– a size #40 K-file; PUI/1– 1 min; PUI/2– 2 min; PUI/3– 3 min; and Control– without CH paste. An elementary chemical microanalysis (SEM/EDS) was performed to quantify the presence of calcium on the dentinal walls. RESULTS: The percentage of calcium was higher in all experimental groups when compared to the control group (P < 0.05); but no differences among them were found (P > 0.05). CONCLUSION: The amount of calcium hydroxide paste on the dentinal walls was not dependent on length of time of ultrasonic activation. SEM/EDS analysis seems to be a reliable method to assess CH paste removal from the root canals.


2020 ◽  
Vol 73 (6) ◽  
pp. 1145-1148
Author(s):  
Maryna A. Goray ◽  
Nataliia G. Gadzhula ◽  
Olena V. Muntian ◽  
Olena L. Cherepakha ◽  
Larysa F. Kurdysh

The aim: To compare the quality of root canal system preparation with the use of manual K-files, machine Protaper Universal and Silk files by in vitro studies. Materials and methods: Root canals preparation in 45 extracted premolars was performed in three groups with 15 teeth in each with K-files, Protaper Universal and Silk files. Transverse sections of the dental root were prepared. Histologically were assessed: amount of sawdust and predentin remaining, the purity degree of root canal walls. Results: When calculating the sawdust amount at the distance of 3 mm from an apex, a high degree of contamination was observed in the manual K-file group: 53.3% versus 33.3% in the Protaper Universal group and against 20.0% in the Silk file group. The amount of predentin after root canal treatment with manual files reached 25-30%. At the distance of 5 mm from the apex the root canals with high and medium purity degree were detected in 86.7% with Silk files and 80.0% with Protaper Universal files used. All predentin was removed when working with Protaper Universal and Silk files. Conclusions: In the histological sections of the root canals treated with K-files, the larger amount of dentine particles and predentin has been revealed than when using machine tools. The largest amount of predentin and dentine were removed with Protaper Universal files. Silk endodontic system is better for treatment of the root canals dentine surface in the apical area compared to Protaper Universal and K-files.


2017 ◽  
Vol 7 (3) ◽  
pp. 54 ◽  
Author(s):  
Celalettin Topbas ◽  
Ozkan Adiguzel

The goal of endodontic treatment is to remove all the vital and necrotic tissues, microorganisms and microbial byproducts from root canal system. This goal can be achieved through chemical and mechanical debridement of root canals. This article narrates the specifics and requirements of the irrigation solutions. Sodium hypochlorite is proposed as the primary irrigant by virtue of its organic tissue dissolution capacity and broad antimicrobial properties. On the other hand, chelation solutions are recommended as auxiliary solutions to remove the smear layer or to hinder its formation on dentin surface. Thus, it's hoped that sealers and root canal fillers can penetrate to dentin tubules and obturate the canals hermetically. There are new studies on traditional irrigants especially on some irrigants that can replace sodium hypoclorite. This article reviews the new irrigants which can be used in future endodontic practice, and their advantages and limitations. Moreover, actions and interactions of recently used irrigants are adverted.   How to cite this article: Topbas C, Adiguzel O. Endodontic Irrigation Solutions: A Review. Int Dent Res 2017;7:54-61.  Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.


Author(s):  
Girish Nanjannawar ◽  
Saquib Mulla ◽  
Divya Gupta ◽  
Sharad Kamat

It is a well-recognised fact that incomplete cleaning, shaping and obturation of root canals will lead to endodontic failure. Mandibular second premolars usually have a single root and a single root canal. The presence of four separate roots and four root canals is quite rare. Hence, a comprehensive knowledge about the normal canal configuration along with its variations becomes an indispensable pre-requisite to achieve the success of endodontic treatment. Authors hereby present a case of a 26-year-old male patient and describe its succesful treatment of the mandibular second premolar with four roots and four root canals. The clinical implications of this paper aim at establishing an accurate diagnosis of the root canal system using diagnostic aids such as angulated radiographs and making use of advanced endodontic instruments for successful retreatment of endodontic therapy (C+ files and NiTi rotary endodontic instruments).


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