scholarly journals Evaluation of the antimicrobial effect of super-oxidized water (Sterilox®) and sodium hypochlorite against Enterococcus faecalis in a bovine root canal model

2010 ◽  
Vol 18 (5) ◽  
pp. 498-502 ◽  
Author(s):  
Giampiero Rossi-Fedele ◽  
José Antonio Poli de Figueiredo ◽  
Liviu Steier ◽  
Luigi Canullo ◽  
Gabriela Steier ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Antimicrobial Effect

The Evaluation of Anti-Microbial-Effect to Enterococcus Faecalis by Hypochlorous Acid Water

2021 ◽  
pp. 1-6
Author(s):  
Yoshishige Yamada
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Colony Formation ◽  
Hypochlorous Acid ◽  
Antimicrobial Effect ◽  
Endodontic Treatment ◽  
High Concentration ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Root Canal Irrigant

Periapical periodontitis is a major disease, and difficult to achieve successful treatment in some cases. Therefore, root canal irrigation is one of the most important processes to successful endodontic treatment. Sodium Hypochlorite (NaOCl) solution is the most commonly used root canal irrigate. However using NaOCl solution on endodontic treatment should be considered its problems such as allergy, serious tissue damage when inadvertently injected into the maxillary sinus or oral mucosa. Therefore, another root canal irrigant that is safe and has a high antimicrobial effect is desirable. In this study, Hypochlorous Acid (HClO) water was used to evaluate the microbicidal efficacy of the Enterococcus faecalis (E. faecalis) and considered the possibility to be a future root canal irrigant candidate. Original (200ppm), 10% diluted (20ppm), 25% diluted (50ppm) and 50% diluted (100ppm) HClO water were used in this study. Each experimental solution was mixed with low concentrations (5×107 CFU/ml) or high concentration (5×109 CFU/ml) of E. faecalis at 30 seconds, 1minute, 3 minutes, 5 minutes respectively. Then they were placed onto BHI agar plates and incubated for 48 h at 37 ºC, followed by counting the number of Colony formation. The results showed that in the condition of 5×107 CFU/ml E.faecalis, no colony formation was shown within 30 seconds by 50 % diluted HClO water. Whereas only original water was attained to inhibit colony formation completely within 30 seconds under the density of 5×109 CFU/ml E, faecalis. In conclusion, 200ppm HClO water has a capacity to anti-microbiological effect against E.faecalis same as NaOCl solution. Keywords: root canal irrigant, hypochlorous acid (HClO) water, Enterococcus faecalis (E. faecalis)

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF DIODE LASER WHEN USED ADJUNCTIVELY WITH SODIUM HYPOCHLORITE, METHYLENE BLUE OR TOLUIDINE BLUE IN ENTEROCOCCUS FAECALIS CONTAMINATED ROOT CANALS

2020 ◽  
pp. 148-151
Author(s):  
N. Shubhashini ◽  
Vinaya Kumar R ◽  
Annapoorna Kini ◽  
Swetha H.B. ◽  
Srikanth Choudhary B.S. ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Toluidine Blue ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Root Canals

AIM: To evaluate antimicrobial effect of diode laser when used adjunctively with sodium hypochlorite, methylene blue or toluidine blue in Enterococcus faecalis inoculated root canals. METHODOLOGY: 70 extracted uniradicular premolars were prepared with ProTaper files and sterilized. 5 were kept aside as negative control (G1) and the remaining 65 were inoculated with Enterococcus faecalis and incubated for 7 days. Following this, 5 were kept as positive control (G2) and the other 60 were randomly distributed into six groups: G3, diode laser irradiation (1.5W) with sodium hypochlorite; G4, diode laser (3W) with sodium hypochlorite; G5, diode laser (1.5W) with methylene blue; G6, diode laser (3W) with methylene blue; G7, diode laser (1.5W) with toluidine blue; G8, diode laser (3W) with toluidine blue. Subsequently, turbidity was assessed and CFU count determined following intracanal sampling and plating. RESULTS: G4 exhibited significantly lower mean CFUs/mL (P˂0.001) among the experimental groups. G4 demonstrated the most (98.8%) while G7 exhibited the least antibacterial effect (28.66%). Highest turbidity was observed in G5 (0.1310) while the lowest was noted in G4 (0.0287). CONCLUSION: Diode laser used adjunctively with sodium hypochlorite exhibited superior antibacterial efficacy when compared to its use with photosensitizer dyes like methylene blue and toluidine blue.

scholarly journals Comparative Study of the Antimicrobial Effect of Three Irrigant Solutions (Chlorhexidine, Sodium Hypochlorite and Chlorhexidinated MUMS)

2012 ◽  
Vol 13 (4) ◽  
pp. 436-439 ◽  
Author(s):  
Hamid Jafarzadeh ◽  
Maryam Bidar ◽  
Sepideh Hooshiar ◽  
Mahboubeh Naderinasab ◽  
Mostafa Moazzami ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Comparative Study ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Lactobacillus Casei ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Canal Irrigation ◽  
The Difference

ABSTRACT Aim To compare the antimicrobial effect of 2% chlorhexidine, 2.5% sodium hypochlorite and MUMS containing 2% chlorhexidine. Materials and methods All of the above irrigants were examined on Enterococcus faecalis, Streptococcus mutans, Candida albicans, Lactobacillus casei and E.coli. A total of 0.5 CC of each solution and 0.5 CC of McFarland solution bacterium were added to each examination tube. After 15, 30 and 45 minutes, colony count was performed for each tube. The difference in the number of bacteria indicated the effect taken by disinfectant material. Results MUMS containing chlorhexidine showed the antimicrobial properties just like chlorhexidine's effect against E.coli, Streptococcus mutans, Candida albicans, Enterococcus faecalis and Lactobacillus casei in preventing these entire microorganisms to incubate. Sodium hypochlorite was not effective against Enterococcus faecalis and Candida albicans incubated in 15, 30 and 45 minutes and Enterococcus faecalis in 15 minutes. Conclusion MUMS has antimicrobial properties similar to chlorhexidine. Clinical significance As MUMS containing chlorhexidine can transfer chlorhexidine through its own surfactant around apical area and it can open the dentinal tubules by its own chelator for more penetration of chlorhexidine, it may be a choice for canal irrigation. How to cite this article Bidar M, Hooshiar S, Naderinasab M, Moazzami M, Orafaee H, Naghavi N, Jafarzadeh H. Comparative Study of the Antimicrobial Effect of Three Irrigant Solutions (Chlorhexidine, Sodium Hypochlorite and Chlorhexidinated MUMS). J Contemp Dent Pract 2012;13(4): 436-439.

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