scholarly journals Occurrence of antibodies to Toxoplasma gondii in rheas (Rhea americana) and ostriches (Struthio camelus) from farms of different Brazilian regions

2013 ◽  
Vol 22 (3) ◽  
pp. 437-439 ◽  
Author(s):  
Amanda Brentano Almeida ◽  
Michelle Mayara Krindges ◽  
Luiz Daniel de Barros ◽  
João Luis Garcia ◽  
Giovana Camillo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Blood Samples ◽  
Rhea Americana ◽  
Struthio Camelus ◽  
Modified Agglutination Test

This study aimed to verify the occurrence of antibodies againstToxoplasma gondii in rheas (Rhea americana) and ostriches (Struthio camelus) commercially breeding in Brazil. Blood samples from 20 rheas and 46 ostriches (young and adults) were serologically tested using a technique known as modified agglutination test (MAT) at an initial titration of 1:16 for ostriches and 1:25 for rheas. Antibodies against T. gondii were found in 50% (10/20) of the rheas, with titers ranging from 1:25 to 1:6,400. The incidence of antibodies against T. gondii in ostriches was 17.4% (8/46) with titers ranging from 1:16 to 1:256. Birds showing titers higher than 1:200 forT. gondii were mainly the young ones. Therefore, rheas and ostriches may be parasitized by T. gondii, showing high levels of antibodies against this parasite.

scholarly journals The prevalence of antibodies to Toxoplasma gondii in horses in Changji Hui Autonomous Prefecture, Xinjiang, northwestern China

2015 ◽  
Vol 24 (3) ◽  
pp. 298-302 ◽  
Author(s):  
Jin-Lei Wang ◽  
Dong-Hui Zhou ◽  
Jia Chen ◽  
Guang-Xue Liu ◽  
Wen-Bing Pu ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Toxoplasma Gondii ◽  
Logistic Regression Analysis ◽  
Multivariate Logistic Regression Analysis ◽  
Agglutination Test ◽  
Northwestern China ◽  
Multivariate Logistic Regression ◽  
Blood Samples ◽  
Modified Agglutination Test

Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii, which can infect warm-blooded animals and humans. The present study was performed to investigate the seroprevalence of T. gondii in horses in Xinjiang, northwestern China. A total of 637 blood samples were collected from seven regions in Changji Hui Autonomous Prefecture, Xinjiang in 2011 and assayed for T. gondiiantibodies using the modified agglutination test (MAT). Risk factors (age, gender, and region) related to seroprevalence were determined by a multivariate logistic regression analysis. A total of 200 horses (31.4%, 95% CI 27.79–35.00) were seropositive for T. gondii. Age, gender, and region present no association with seroprevalence (p>0.05) in the logistic regression analysis. The results indicated that T. gondii is widely prevalent in horses in Xinjiang, northwestern China, representing a serious threat to animal and human health. Therefore, more careful measures should be performed to control and prevent T. gondii infection in horses from Xinjiang, northwestern China.

scholarly journals Validation of the Use of Dried Blood Samples for the Detection of Toxoplasma gondii Antibodies in Stray Cats (Felis s. catus)

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 864
Author(s):  
Julie Simon ◽  
Dominique Aubert ◽  
Régine Geers ◽  
Isabelle Villena ◽  
Marie-Lazarine Poulle
Keyword(s):  
Toxoplasma Gondii ◽  
Kappa Coefficient ◽  
Agglutination Test ◽  
Sample Type ◽  
Blood Samples ◽  
Blotting Paper ◽  
Modified Agglutination Test ◽  
Stray Cats ◽  
Free Ranging ◽  
Dilution Level

If validated beforehand, the analysis of dried blood on blotting paper (BP samples) is very useful for monitoring free-ranging animals. We aimed to validate this method for the detection of antibodies against Toxoplasma gondii in stray cats. We used the modified agglutination test (MAT) in 199 sample pairs of sera and BP samples from 54, 39, 56, and 50 cats trapped during four periods in five dairy farms. Screening was at 1:6, 1:12, and 1:24 dilutions. The cut-off value was at MAT titre ≥ 24, but MAT titre ≥ 12 was also considered for BP samples that often have a higher dilution level. Depending on the period, sample type, and cut-off value, sensitivity of the analysis of the BP sample vs. serum varied from 87.1% to 100% and specificity ranged from 72.22% to 100%. The concordance values and Kappa coefficient showed a substantial to excellent agreement between the results of the two methods, whatever the cut-off value. These findings quantifiably validate the use of MAT on BP samples for the detection of antibodies to T. gondii in stray cats, but we recommend expressing results from BP samples with several cut-off values as the MAT titres tend to be lower than those of sera.

scholarly journals Prevalence of Toxoplasma gondii and Neospora caninum infections in households sheep "Elevage en case" in Dakar, Senegal

2019 ◽  
Vol 12 (7) ◽  
pp. 1028-1032 ◽  
Author(s):  
Laibané Dieudonné Dahourou ◽  
Oubri Bassa Gbati ◽  
Madi Savadogo ◽  
Bernadette Yougbare ◽  
Amadou Dicko ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Significant Variation ◽  
Neospora Caninum ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Sheep Breeding ◽  
Modified Agglutination Test ◽  
Husbandry System

Aim: The study aimed to evaluate the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep breeding in a particular husbandry system called "Elevage en case" in Dakar, Senegal. Materials and Methods: Blood samples were collected from 278 sheep. Serum was harvested and used for analysis. For the detection of T. gondii antibodies, 278 sera were analyzed using the modified agglutination test, while the enzyme linked-immunosorbent assay was used on 174 sheep sera to look for N. caninum antibodies. Results: This study showed that 29.4±6.8% of sheep carried both T. gondii and N. caninum antibodies. The overall prevalence was 60.1±5.7% and 41.9±7.3% for toxoplasmosis and neosporosis, respectively. For toxoplasmosis, the prevalence was higher in Gueule Tapée (63.3%) than in Medina (58.9%), but the variation was not significant (p=0.45). Regarding the age of animals, the prevalence was significantly higher in animals over 2 years old compared to those under 2 years old (p=0.002). For neosporosis, the prevalence was significantly higher in Medina (48.67%) than Gueule Tapée (16.7%) (p=0.001), but non-significant variation was noted according to animal age. Conclusion: The study showed that sheep reared in households have carried antibodies of T. gondii and N. caninum. The prevalence was high and it means that consumption of meat from these animals is risky if the meat is eaten undercooked.

scholarly journals Detection of Toxoplasma gondii in a free-ranging giant anteater

2017 ◽  
Vol 47 (8) ◽  
Author(s):  
Thais Oliveira Morgado ◽  
Francielle Cristina Kagueyama ◽  
Janaina Marcela Assunção Rosa ◽  
Melissa Debesa Belizário ◽  
Richard de Campos Pacheco ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Zoonotic Infection ◽  
Chronic Infections ◽  
Chain Reaction ◽  
Positive Serology ◽  
Modified Agglutination Test ◽  
Polymerase Chain ◽  
Free Ranging ◽  
First Time

ABSTRACT: Toxoplasmosis is caused by Toxoplasma gondii, an obligatory intracellular protozoan, which establishes acute and chronic infections in birds and mammals, including humans. This note reports, for the first time, the detection and sequencing of DNA from T. gondii in the peripheral blood of a young free range giant anteater (Myrmecophaga tridactyla). For the diagnosis, the following methods were used: polymerase chain reaction (PCR) and positive serology (1:800) by means of the modified agglutination test (MAT). Since this species may be consumed by humans and predated by wild felids, its importance is emphasized as a probable source of zoonotic infection, in addition to its possible participation in the infection enzootic cycle. Although, parasitemia has been confirmed in this specimen, it presented no clinical sign of infection.

scholarly journals SURVEI SEROLOGIS TOXOPLASMOSIS PADA TERNAK SAPI DI KABUPATEN PRINGSEWU MENGGUNAKAN METODE TOXOPLASMA MODIFIED AGGLUTINATION TEST (To-MAT)

2019 ◽  
Vol 7 (3) ◽  
pp. 270
Author(s):  
Galang B. ◽  
Emantis R. ◽  
Gina Dania P. ◽  
Endah S.
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Test Method ◽  
Cattle Population ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

The cattle population in Lampung Province has a relatively high number when compared to several other provinces. Pringsewu is one of the districts in Lampung Province having the supplier potential of beef in Lampung. The higher the population and production, could cause a potential infection of toxoplasmosis to livestock and human. So far the prevalence of toxoplasmosis in Pringsewu District. This study was conducted to determine the level of toxoplasmosis infection in cattle in Pringsewu district. This study used the To-MAT (Toxoplasma Modified Agglutination Test) method to test Toxoplasma gondii infection in blood of cows samples. The result of the study found that at a chronic level there were 23/24 cows positively infected with T.gondii. Whereas in acute level there are 8/24 cows positively infected with T. gondii. In general, the prevalence of toxoplasmosis is 95.8%. Keywords : Toxoplasmosis, Cow, To-MAT (Toxoplasma Modified Agglutination Test) method, Infection

scholarly journals First report on seroprevalence and risk factors of Toxoplasma gondii infection in sheep and goats in North Lebanon

2019 ◽  
Vol 13 (09) ◽  
pp. 831-836 ◽  
Author(s):  
Dima El Safadi ◽  
Dany Abi Chahine ◽  
Alissar Al Tarraf ◽  
Omar Raii ◽  
Karim Mesto ◽  
...  
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Toxoplasma Gondii ◽  
Immunoglobulin G ◽  
Agglutination Test ◽  
Economic Losses ◽  
Transplacental Transmission ◽  
Igg Antibodies ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

Introduction: Toxoplasmosis is of dual importance in both public and veterinary health due to the respective risk of transplacental transmission in primo-infected pregnant women and economic losses caused by abortions in mammals. One of the main routes of Toxoplasma gondii transmission to humans is the consumption of raw or undercooked meats containing parasitic cysts. Here, we performed the first epidemiological study to determine the seroprevalence and the risk factors of toxoplasmosis in livestock in Lebanon. Methodology: Using a modified agglutination test with a cut-off of 1:40, we tested the positivity rate of Immunoglobulin G antibodies in the sera of 100 sheep and 80 goats collected from 18 different livestock farms located in North Lebanon between March and June 2018. Results: Anti-Toxoplasma gondii IgG antibodies were detected in 42% of sheep and 34% of goats. Adults (> 1 year) were significantly more infected by T. gondii than the lambs (< 1 year) in both species (p < 0.05). Conclusions: These findings indicated that food animals are highly exposed to T. gondii in Lebanon and could be potentially a major risk factor of T. gondii infection to humans. Consequently, national prophylactic strategies should be implemented to control and to prevent T. gondii transmission between animals and humans.

scholarly journals Serological diagnosis of Toxoplasmosis by specific antibody in camels of Wasit province, Iraq

2018 ◽  
Vol 1 (29) ◽  
pp. 412-423
Author(s):  
Basim Mohammed Hanon
Keyword(s):  
Public Health ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Zoonotic Parasite

Background: toxoplasma gondii is a zoonotic parasite, more added a major public health is worldwide because have high distribution in livestock. Objectives: the main aim of this study determine the occurrence of the seroepidemiological toxoplasmosis in camels in waist province of Iraq from November 2016 to April 2017. Materials and Methods: blood samples collected of animals randomly were included six different groups of animals were diagnosed by A Latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) kits.

scholarly journals Seroprevalence of Toxoplasma gondii and Leishmania spp. in domestic donkeys from Portugal

2019 ◽  
Vol 28 (1) ◽  
pp. 172-176 ◽  
Author(s):  
Filipa Teixeira Rodrigues ◽  
Catarina Pereira ◽  
Jitender Prakash Dubey ◽  
Miguel Nóvoa ◽  
Miguel Quaresma ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Leishmania Infantum ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Serum Samples ◽  
Modified Agglutination Test ◽  
Leishmania Spp

Abstract Toxoplasma gondii and Leishmania infantum are zoonotic protozoal parasites. Serum samples were obtained from 186 donkeys (Equus africanus asinus) from Portugal and assessed for antibodies to T. gondii by the modified agglutination test (MAT). For titration of antibodies to Leishmania spp. the direct agglutination test was used (DAT). Eleven donkeys were seropositive for T. gondii with titres of 20 (n = 7), 80 (n = 2), 640 (n = 1) and ≥ 2560 (n = 1). One donkey was seropositive for Leishmania spp. (titre of 800). Donkeys in Portugal are exposed to and can be infected with T. gondii and Leishmania spp.

scholarly journals Immunohistochemical identification of Toxoplasma gondii in tissues from Modified Agglutination Test positive sheep

2013 ◽  
Vol 191 (3-4) ◽  
pp. 347-352 ◽  
Author(s):  
A.F. Silva ◽  
F.C.R. Oliveira ◽  
J.S. Leite ◽  
M.F.V. Mello ◽  
F.Z. Brandão ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Modified Agglutination Test ◽  
Immunohistochemical Identification

scholarly journals Toxoplasma gondii survey in cats from two environments of the city of Rio de Janeiro, Brazil by Modified Agglutination Test on sera and filter-paper

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Paula F. Bolais ◽  
Philippe Vignoles ◽  
Pamela F. Pereira ◽  
Rafael Keim ◽  
Abdelkrim Aroussi ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Filter Paper ◽  
Rio De Janeiro ◽  
Agglutination Test ◽  
Modified Agglutination Test ◽  
The City
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