scholarly journals Trypanosomatids (Protozoa: Kinetoplastida) in three species of Armored Catfish from Mogi-Guaçu river, Pirassununga, São Paulo, Brazil

2016 ◽  
Vol 25 (2) ◽  
pp. 131-141 ◽  
Author(s):  
Julia Pereira Molina ◽  
Rubens Riscala Madi ◽  
Vera Nisaka Solferini ◽  
Paulo Sérgio Ceccarelli ◽  
Hildete Prisco Pinheiro ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Sao Paulo ◽  
Average Density ◽  
Molecular Techniques ◽  
Morphological Data ◽  
São Paulo ◽  
Significant Difference ◽  
General Average ◽  
Monthly Variations ◽  
Formation Of Groups

Abstract Trypanosome infections have been reported in several species of fish, in majority of cases described on the basis of morphological characteristics. Trypanosomes in fish are heteroxenous and transmitted by hirudineans. This study aims to evaluate the prevalence and density of infections by Trypanosoma sp. in blood from three species of catfish, Hypostomus regani, H. strigaticeps, H. albopunctatus, from the Mogi Guaçu River, Pirassununga, São Paulo, Brazil. Further, this study intends to characterize the Trypanosoma specimens found in the blood of these fish by morphological and molecular techniques. The trypanosomes overall prevalence observed was 47.6% with a general average density of 0.75 parasites/µl of blood. Hypostomus regani and Hypostomus strigaticeps showed a significant difference in prevalence. The average densities of parasites were not significantly different among the three fish species. Similar findings were observed for the monthly variations in densities. The parasites found in the three species of catfish studied showed similar morphological characteristics. The morphological data and the statistical analyses used in this study didn’t show the formation of groups. The analyses provided evidence of the presence of pleomorphisms in the trypanosomes found in the three studied fish.

Checklist of decapods (Crustacea) from the coast of the São Paulo state (Brazil) supported by integrative molecular and morphological data: I. Infraorder Caridea: families Hippolytidae, Lysmatidae, Ogyrididae, Processidae and Thoridae

Zootaxa ◽  
2018 ◽  
Vol 4370 (1) ◽  
pp. 76 ◽  
Author(s):  
MARIANA TEROSSI ◽  
ALEXANDRE O. ALMEIDA ◽  
RAQUEL C. BURANELLI ◽  
ANTONIO L. CASTILHO ◽  
ROGÉRIO C. COSTA ◽  
...  
Keyword(s):  
First Record ◽  
Molecular Data ◽  
Color Pattern ◽  
Sao Paulo ◽  
South Atlantic ◽  
Molecular Techniques ◽  
Morphological Data ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State

The current checklist is the result of a long-term multidisciplinary project which combined molecular techniques (mitochondrial DNA markers) and morphological analyses of adult specimens for an accurate and detailed identification of the total biodiversity of decapod crustaceans from marine and coastal (including estuaries) environments of São Paulo State (Brazil). This is the first of a series of reports and providing a checklist of caridean shrimps of the families Hippolytidae (5 spp.), Lysmatidae (6 spp.), Ogyrididae (2 spp.), Processidae (5 spp.) and Thoridae (1 sp.). We collected material of 13 species out of 19 recorded, with sequences of cytochrome oxidase subunit I - barcode region and 16S generated from 10 species. The previous record of Lysmata cf. intermedia for São Paulo is actually L. jundalini, as the first record in São Paulo/South Atlantic waters. The molecular data were helpful to confirm the identification of some species, as the occurrence of L. wurdemanni which is confirmed in the South Atlantic Ocean based on morphological, color pattern and molecular data. 

Checklist of decapod crustaceans from the coast of the São Paulo state (Brazil) supported by integrative molecular and morphological data: III. Infraorder Brachyura Latreille, 1802

Zootaxa ◽  
2020 ◽  
Vol 4872 (1) ◽  
pp. 1-108
Author(s):  
FERNANDO L. MANTELATTO ◽  
ANA FRANCISCA TAMBURUS ◽  
TATIANA MAGALHÃES ◽  
RAQUEL C. BURANELLI ◽  
MARIANA TEROSSI ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Molecular Techniques ◽  
Morphological Data ◽  
Brazilian Coast ◽  
São Paulo ◽  
Decapod Crustaceans ◽  
Neohelice Granulata ◽  
Paulo State ◽  
São Paulo State ◽  
First Time

This checklist is the third part of a series derived from a long-term multidisciplinary project on the biodiversity of decapod crustaceans from marine and coastal environments of São Paulo state (Brazil). We integrated molecular techniques (DNA markers) and morphological analyses of adult specimens for accurate identifications. We compilated 185 species from the literature, but we confirmed the presence of 168 species: 130 of which we sampled, analyzed and obtained sequences (COI and/or 16S totalizing 113 sequences) and 38 that were not directly collected but were confirmed by analyses. In addition, 27 had their distribution removed from São Paulo due to uncertainties, and absence of material as voucher. Five species were reported for the first time on the coast of São Paulo (Calappa ocellata, Neohelice granulata, Teleophrys pococki, Teramnonotus monodi, Tetraxanthus rathbunae) and one on the Brazilian coast (Pseudomedaeus agassizi). Most of the non-sampled species previously reported on the coast of São Paulo might be considered doubtful records stablished in the past by inaccurate analyses, which assumed extended distribution to the area and/or misidentifications. At this time and based on our refined compilation, we can estimate the brachyuran diversity on the coast of São Paulo in 168 species. This detailed inventory contributes to the knowledge on the local decapod fauna by checking available dataset, adding new species records in São Paulo and new sequences to GenBank database. These data may serve as baseline for future identifications and studies on conservation, population genetics, biogeography and phylogenetics, which might flag species that deserve further investigations and concerns. 

Checklist of decapods (Crustacea) from the coast of São Paulo State (Brazil) supported by integrative molecular and morphological data: II. Infraorder Caridea: family Alpheidae

Zootaxa ◽  
2018 ◽  
Vol 4450 (3) ◽  
pp. 331 ◽  
Author(s):  
ALEXANDRE O. ALMEIDA ◽  
MARIANA TEROSSI ◽  
RAQUEL C. BURANELLI ◽  
FERNANDO J. ZARA ◽  
FERNANDO L. MANTELATTO
Keyword(s):  
Dna Markers ◽  
Sao Paulo ◽  
Molecular Techniques ◽  
Morphological Data ◽  
Morphological Identification ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State ◽  
First Time

This study is part of a series of checklists resulting from a long-term multidisciplinary project on the biodiversity of decapod crustaceans from the marine and coastal environments (including estuaries) of São Paulo State (Brazil). For that, we integrated molecular techniques (mitochondrial DNA markers) and morphological analyses of adult specimens for an accurate and detailed identification. The DNA markers were used when the morphological identification was doubtful, particularly in the recognition of cryptic species. This second manuscript presents a checklist of the Alpheidae caridean shrimps from the coast of São Paulo. We report the occurrence of Alpheus cf. paracrinitus and Synalpheus townsendi for the first time in the region. Based on our survey, 39 species of Alpheidae are known for this region: Alpheus (21 spp.), Athanas (2 spp.), Automate (2 spp.), Leptalpheus (1 spp.), Salmoneus (3 spp.), and Synalpheus (10 spp.). We collected 28 species and obtained cytochrome oxidase subunit I (barcode region) and/or 16S partial sequences of 26 of them. These sequences may be used for phylogenetic and populational analyses in further studies. 

Checklist of decapod crustaceans from the coast of the São Paulo state (Brazil) supported by integrative molecular and morphological data: IV. Infraorder Anomura: Superfamilies Chirostyloidea, Galatheoidea, Hippoidea and Paguroidea

Zootaxa ◽  
2021 ◽  
Vol 4965 (3) ◽  
pp. 558-600
Author(s):  
FERNANDO L. MANTELATTO ◽  
IVANA MIRANDA ◽  
ANA L. VERA-SILVA ◽  
MARIANA NEGRI ◽  
RAQUEL C. BURANELLI ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Molecular Techniques ◽  
Morphological Data ◽  
São Paulo ◽  
Decapod Crustaceans ◽  
Cytochrome Oxidase Subunit ◽  
Accurate Identification ◽  
Paulo State ◽  
São Paulo State

This checklist is the fourth contribution resulting from a long-term multidisciplinary project which combined morphological analyses and molecular techniques (mitochondrial DNA markers) for accurate identification of marine and coastal decapod crustaceans of São Paulo State (Brazil). We provide a list of 63 species of the following 11 families of 4 superfamilies of Anomura: Albuneidae (4 spp.), Blepharipodidae (1 sp.), Chirostylidae (1 sp.), Diogenidae (18 spp.), Hippidae (1 sp.),  Munididae (8 spp.), Munidopsidae (1 sp.), Paguridae (13 spp.), Parapaguridae (2 spp.), Porcellanidae (13 spp.), and Pylochelidae (1 sp.). Seven species previously reported from the region were neither collected nor found in museum collections during our study, including one (Sympagurus dimorphus) that we suggest to be removed from São Paulo coast fauna lists. We generated new sequences of cytochrome oxidase subunit I (barcode region) and 16S genes of 44 species. This anomuran inventory may serve as guideline for future studies on taxonomy, conservation, population genetics, biogeography, and phylogenetics, which might flag species that deserve further investigations and concerns. 

scholarly journals Behavior of gastric cancer in Brazilian population

2009 ◽  
Vol 22 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Carlos Eduardo Jacob ◽  
Claudio Bresciani ◽  
Joaquim José Gama-Rodrigues ◽  
Osmar Kenji Yagi ◽  
Donato Mucerino ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Resection ◽  
Gastric Wall ◽  
Age Groups ◽  
Sao Paulo ◽  
São Paulo ◽  
Gender Ratio ◽  
Male Predominance ◽  
Number Of Patients ◽  
Significant Difference

BACKGROUND: Gastric cancer (GC) is a predominately male disease. Usually for every female that suffers from this condition there are two males and occurred an increase in the number of females in last decades. Brazil is poor in data about this issue. AIM: To verify if in Brazil it happened: a) a change in the gender ratio and on the average age of the patients; b) an increase in the number of patients with 70 years of age or more suffering from this disease; c) changes in the gender ratio and in the average age in the several gastric locations during the period of study. METHODS: The medical history of patients diagnosed with primary gastric adenocarcinoma, between 1971 and 1998 were obtained at Hospital das Clínicas of the University of São Paulo, São Paulo, SP, Brazil. Exclusion criteria were: patients suffering from a non epithelial gastric malignancy; adenocarcinoma from the intestinal metaplasia in the distal esophagus invading the proximal stomach and patients submitted to a gastric resection, due to a benign or malignant tumor during the last five years prior to the surgical procedure analyzed in this study. The patients were divided into 10 years age groups and also divided in three groups, according to their ages and time intervals. Interrelationships between gender and age, and with tumor´s location on gastric wall were analyzed. RESULTS: From 1971 to 1998, 1578 patients with GC were hospitalized. Among them, 1021 were treated with gastric resection, corresponding to 64.7% of all patients. There was an increase in the proportion of patients older than 70 years, and decrease between 41 and 70 years. There was no statistical significant difference among the average ages and the different locations. There were significant differences for the locations favoring proximal third and stump, both more prevalent in males. CONCLUSIONS: a) Occurred modifications in the ratio between genders: greater number of women and an increase in the number of male patients in the age group between 41 and 70 years; b) it was proved the greater number of occurrences in patients over 70 years of age; c) there was a greater increase in the male predominance in the tumors located in the stomach´s proximal third.

scholarly journals Density and diversity of filamentous fungi in the water and sediment of Araçá bay in São Sebastião, São Paulo, Brazil

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Sonia Assami Doi ◽  
Aline Bartelochi Pinto ◽  
Maria Carolina Canali ◽  
Daiane Raquel Polezel ◽  
Roberta Alves Merguizo Chinellato ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Chemical Properties ◽  
Morphological Characteristics ◽  
Sao Paulo ◽  
Northern Coast ◽  
São Paulo ◽  
Mangrove Swamp ◽  
Sediment Samples ◽  
Water And Sediment ◽  
Pharmaceutical Industries

Abstract Araçá Bay, located in the city of São Sebastião, São Paulo, Brazil, is a protected area of substantial complexity. It represents the last remaining mangrove swamp preserve between the cities of Bertioga and Ubatuba on the northern coast of São Paulo State. This mangrove swamp has specific physical and chemical properties, and it shelters a wide variety of life, including fungi. These microorganisms are present in a variety of species with different morphophysiological features, and they have the ability to produce enzymes of biotechnological importance. The goal of this study was to quantify, isolate, and identify filamentous fungi in water and sediment samples from the Araçá Bay mangrove swamp in São Sebastião. Two samplings were performed in the summer and two were performed in the winter. The samples were collected from intertidal zones, and dissolved oxygen (DO), temperature, salinity, and pH were measured in situ. The spread plate technique was used to inoculate the samples collected on plates with a potato dextrose agar (PDA) medium. A total of 208 colonies (68 from water samples and 140 from sediment samples) were isolated, and they were identified based on their morphological characteristics. Filamentous fungus density was higher in the sediment than in the water, and the samplings performed in the winter revealed a higher density than those performed in the summer. Though some of the environmental parameters were not ideal for fungal development, a high quantity of growth was nevertheless observed. When the isolated colonies were analyzed, the greatest diversity and species richness were found in the summer samples. The genera identified in all of the samples were Aspergillus, Penicillium, Cladosporium, and Fusarium. The pathogenic species found from these genera were Aspergillus fumigatus, A. terreus, Penicillium citrinum, and P. chrysogenum. These species are also able to produce enzymes that offer a variety of applications. The fungal community described herein represents the diversity found in this mangrove swamp during the period studied. Many of the fungus species found are pathogenic and may be useful due to their ability to produce specific enzymes applicable in the biotechnological and pharmaceutical industries.

scholarly journals Morphological divergence of seedlings Calophyllum brasiliense cambes collected in São Paulo and Tocantins

2021 ◽  
Author(s):  
Ildon Rodrigues Nascimento ◽  
Cândida Pereira da Silva ◽  
Irais Dolores Pascual-Reyes ◽  
Aline Torquato Tavares ◽  
Edilson Nonato da Silva ◽  
...  
Keyword(s):  
Root Length ◽  
Quality Index ◽  
Morphological Characteristics ◽  
Dry Mass ◽  
Sao Paulo ◽  
The State ◽  
Morphological Divergence ◽  
São Paulo ◽  
Calophyllum Brasiliense ◽  
Number Of Leaves

Objective: The objective was to quantify the morphological diversity of Calophyllum brasiliense Cambes from four municipalities of the State of Tocantins employing morphological characteristics, to strengthen information on conservation and future breeding of the species. Methodology: Seeds were collected from four municipalities in the State of Tocantins and one in the State of São Paulo. The following were evaluated: plant height, stem diameter, root length, number of leaves, leaf area, root dry mass, shoot dry mass, total dry mass, and Dickson´s quality index. The data were subjected to univariate analysis of variance, Tocher grouping method, and UPGMA, obtaining a dendrogram through the generalized Mahalanobis distance. Results: The results showed a statistical difference of 1 and 5% probability. Dueré stood out in stem diameter(5.52 mm), Sandolandia in height (34.84 cm) and root length (42.13 cm). Formoso stood out in the number of leaves (34 leaves). Lagoa da Confusão in leaf area (856.28 cm 2 ) and São Paulo in root dry mass (16.20 g), shoot dry mass (12.38 g), total dry mass (16.20 g), and Dickson´s quality index (1.57). Implications: Variations in morphological characteristics can be used as a tool for genetic studies of guanandi progeny accordingto their similarity and/or differences. Conclusions: The morphological divergence evidenced that among the five studied areas it is possible to direct the collection of seeds to subsidize conservation strategies and future breeding of the species.

scholarly journals A new species of Lizerius Blanchard (Hemiptera: Drepanosiphidae) from Brazil

2019 ◽  
Vol 109 ◽  
Author(s):  
Suzan B. Z. Cunha ◽  
Carlos R. Sousa-Silva
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Aphid Species ◽  
Sao Paulo ◽  
Persea Americana ◽  
São Paulo ◽  
A New Species

ABSTRACT In this study a new aphid species of the genus Lizerius Blanchard, 1923 (Hemiptera: Drepanosiphidae) is described. Samplings were carried out in the municipalities of Porto Ferreira and Pedregulho, state of São Paulo, Brazil, over plants of Persea americana Mill, 1768 and Terminalia brasiliensis Spreng, 1825. Morphological characteristics of apterous and alate are described and represented by drawings.

Prevalence of Upper Cervical Vertebrae Anomalies in Patients with Cleft Lip and/or Palate and Noncleft Patients

2009 ◽  
Vol 46 (5) ◽  
pp. 481-486 ◽  
Author(s):  
Marta Cunha Lima ◽  
Eduardo Jacomino Franco ◽  
Guilherme Janson ◽  
Izabel Maria Marchi Carvalho ◽  
Carlos Ferreira Santos ◽  
...  
Keyword(s):  
Cleft Lip ◽  
Cervical Vertebrae ◽  
Sao Paulo ◽  
Posterior Arch ◽  
São Paulo ◽  
Craniofacial Anomalies ◽  
Lateral Cephalograms ◽  
Significant Difference ◽  
Upper Cervical ◽  
School Of Dentistry

Objectives: To investigate the prevalence of upper cervical vertebrae anomalies in patients with isolated cleft lip, isolated cleft palate, and complete cleft lip and palate, as well as to compare the prevalence of these anomalies between groups, between genders, and with noncleft patients. Design: Retrospective cross-sectional study of randomly selected patients. Setting: Radiology Section, Hospital for Rehabilitation of Craniofacial Anomalies and Department of Orthodontics, Bauru School of Dentistry, University of São Paulo, São Paulo, Brazil. Participants: The sample comprised 300 lateral cephalograms of cleft patients, aged 12 to 13 years, of both genders, from the files of the Hospital for Rehabilitation of Craniofacial Anomalies–University of São Paulo and 300 lateral cephalograms from noncleft patients of the Department of Orthodontics, Bauru School of Dentistry. Cephalograms of patients with syndromes were not included. Method: Radiographs were interpreted on a film viewer by a single examiner; the profiles of vertebrae were traced on acetate paper, and cervical vertebrae anomalies were registered and categorized into posterior arch deficiencies, fusion, and association of both. Main Outcome Measures: Statistical comparison of groups using the chi-square test. Results: In the cleft group, 38.67% of the patients had cervical vertebrae anomalies. Of those in the noncleft sample, 31% showed anomalies of the cervical spine. This difference was statistically significant. There was no statistically significant difference when the types of clefts were compared with each other or when both genders were compared in both samples. Conclusions: This study confirms the association between clefts and cervical anomalies. Additional research on this topic is necessary.

Prevalence of Palatal and Alveolar Cysts in Babies with Cleft Lip and Palate

2004 ◽  
Vol 41 (5) ◽  
pp. 490-493 ◽  
Author(s):  
Karina Mirela Ribeiro Pinto Alves ◽  
Virginia Peixoto ◽  
Márcia Ribeiro Gomide ◽  
Cleide Felíciode Carvalho Carrara ◽  
Beatriz Costa
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Sao Paulo ◽  
São Paulo ◽  
Craniofacial Anomalies ◽  
Cross Sectional ◽  
Significant Difference ◽  
Different Types ◽  
Low Prevalence ◽  
Posterior Area

Objective To evaluate the prevalence of palatal and alveolar cysts in babies with cleft lip and/or palate. Design Cross-sectional. Setting Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo (HRAC-USP), Bauru, São Paulo, Brazil. Participants Two hundred ninety-one Caucasian babies divided into four groups according to the type of cleft: cleft lip with or without cleft alveolus (70), complete unilateral cleft lip and palate (112), complete bilateral cleft lip and palate (56), and cleft palate (53). Results A low prevalence of palatal and alveolar cysts was observed among patients with the four different types of clefts, with no statistically significant difference between genders. The maxilla and the anterior area of the mouth were more affected than the mandible and the posterior area. Conclusions The low prevalence of palatal and alveolar cysts in the four groups of babies with clefts included in this study may have been due to the high mean age of the sample.

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