scholarly journals Molecular survey and genetic diversity of piroplasmids in equids from Midwestern Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 464-472 ◽  
Author(s):  
Fabio Bernardo Schein ◽  
Maerle Oliveira Maia ◽  
Rute Witter ◽  
Arlei Marcili ◽  
Lázaro Manoel de Camargo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Maximum Parsimony ◽  
Geographic Origin ◽  
Intraspecific Variability ◽  
Theileria Equi ◽  
Mato Grosso ◽  
Babesia Caballi ◽  
Blood Samples ◽  
Phylogenetic Studies

Abstract We evaluated the distribution of piroplasmids in equids from the Mato Grosso state in Midwestern Brazil using molecular methods and the interspecific genetic diversity. For this, 1,624 blood samples of equids from 973 farms were examined by PCR, using primer pairs that amplify a fragment of the genes rap-1 and ema-1 of Babesia caballi and Theileria equi, respectively. For molecular characterization and phylogenetic studies, 13 and 60 sequences of the rap-1 and ema-1 genes, respectively, were used to build a dendogram using maximum parsimony. B. caballi and T. equi were detected in 4.11% and 28.16% of the farms, respectively, and molecular prevalence was 2.74% for B. caballi and 25.91% for T. equi. The location of the farms and animals raised in the Pantanal ecoregion influence the probability of equids testing positive for B. caballi and T. equi . Moreover, age and herd purpose were variables significantly associated with T . equi infection. The sequences of B. caballi presented 1.95% intraspecific variability, contrasting with 2.99% in T. equi. Dendrograms for both species demonstrated the presence of subgroups with high values of support of branches. However, it is not possible to associate these groups with geographic origin and/or ecoregion.

scholarly journals Investigating the presence of equine piroplasmosis in Ireland

2020 ◽  
Vol 187 (11) ◽  
pp. e97-e97
Author(s):  
Robert M Coultous ◽  
Desmond P Leadon ◽  
Brian R Shiels ◽  
David Sutton ◽  
William Weir
Keyword(s):  
Genetic Diversity ◽  
Ribosomal Rna ◽  
Dna Sequences ◽  
Parasite Species ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Blood Samples ◽  
Equine Piroplasmosis ◽  
The Uk ◽  
Pcr Targeting

BackgroundEquine piroplasmosis (EP) is a notifiable disease in Ireland and a significant concern to domestic and international equine industries. Information regarding EP presence in Ireland is currently limited. This retrospective surveillance study describes a serological and molecular analysis of blood samples submitted to the Irish Equine Centre for EP testing between January 2013 and April 2016.MethodsFollowing serological testing, seropositive samples were screened using a PCR targeting the 18S ribosomal RNA gene. Amplicon sequences were bioinformatically analysed to identify the parasite species and to assess genetic diversity.ResultsFrom 2099 screened equine blood samples, 2.5 per cent and 1 per cent were seropositive for Theileria equi and Babesia caballi, respectively. T equi DNA was detected in 9 per cent of the seropositive samples while B caballi DNA was not detected in any sample. The T equi DNA sequences displayed no genetic diversity at this locus, in contrast to samples from the UK and from endemic areas.ConclusionDetection of EP-seropositive and parasitaemic horses in Ireland indicates a clear and present health risk to the equine population. It is recommended that owners adopt appropriate biosecurity measures and that clinicians are mindful of this disease as a differential diagnosis.

The first molecular detection and genetic diversity of Babesia caballi and Theileria equi in horses of Gansu province, China

2019 ◽  
Vol 10 (3) ◽  
pp. 528-532 ◽  
Author(s):  
Jinming Wang ◽  
Junlong Liu ◽  
Jifei Yang ◽  
Xiaoxing Wang ◽  
Zhi Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Detection ◽  
Gansu Province ◽  
Theileria Equi ◽  
Babesia Caballi

Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey

2017 ◽  
Vol 62 (1) ◽  
Author(s):  
Esin Guven ◽  
Hamza Avcioglu ◽  
Ahmet Deniz ◽  
İbrahim Balkaya ◽  
Ugur Abay ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Equine Piroplasmosis ◽  
Worldwide Distribution ◽  
Tick Borne Disease

AbstractEquine piroplasmosis (EP) is a hemoprotozoan tick-borne disease with worldwide distribution that is caused by

Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil

2007 ◽  
Vol 102 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Alexandra Heim ◽  
Lygia M. F. Passos ◽  
Múcio F. B. Ribeiro ◽  
Lívio M. Costa-Júnior ◽  
Camila V. Bastos ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Endemic Areas

scholarly journals Occurrence and Genetic Diversity of Babesia caballi and Theileria equi in Chilean Thoroughbred Racing Horses

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 714
Author(s):  
Reinaldo Torres ◽  
Claudio Hurtado ◽  
Sandra Pérez-Macchi ◽  
Pedro Bittencourt ◽  
Carla Freschi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rrna Genes ◽  
Serum Samples ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Thoroughbred Horses ◽  
18S Rrna Genes ◽  
Thoroughbred Racing ◽  
First Time ◽  
Rhoptry Protein

This study aimed to serologically and molecularly survey Babesia caballi and Theileria equi in thoroughbred horses from racecourses in Chile. Additionally, the genetic diversity of the positive samples was assessed. A total of 286 thoroughbred horses from the Santiago and Valparaíso racecourses had their serum samples submitted to an ELISA for B. caballi and T. equi, and 457 samples (from the Santiago, Valparaíso, and Concepción racecourses) were tested with nested PCRs for the B. caballi 48 KDa rhoptry protein (RAP-1) and T. equi 18S rRNA genes. Selected RAP-1 and 18S positive products were sequenced to perform phylogenetic and haplotype analyses. An overall seroprevalence of 35.6% was observed for these Chilean racecourses: 23.7% for T. equi, 8.4% for B. caballi, and 3.5% for both agents. Overall, a 53.6% occurrence by nPCR was detected for the three Chilean racecourses: 44.2% for T. equi, 5.4% for B. caballi, and 3.9% for both agents. Phylogenetic analysis of T. equi and B. caballi showed genetic proximity with sequences previously detected in other countries. Haplotype analysis revealed a low diversity among the Chilean sequences, which may have originated from those reported in Brazil, Israel, or Cuba. Babesia caballi and T. equi were detected for the first time in Chilean thoroughbred horses.

scholarly journals Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil

2017 ◽  
Vol 37 (12) ◽  
pp. 1416-1422 ◽  
Author(s):  
Rita de Maria Seabra Nogueira ◽  
Arannadia Barbosa Silva ◽  
Tayra Pereira Sato ◽  
Joicy Cortez de Sá ◽  
Ana Clara Gomes dos Santos ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Animal Health ◽  
Antibody Test ◽  
18S Rrna Gene ◽  
Boophilus Microplus ◽  
Rrna Gene ◽  
Serum Samples ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Blood Samples

ABSTRACT: Equine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area.

Genetic diversity of Theileria equi and Babesia caballi infecting horses of Central-Southern Italy and preliminary results of its correlation with clinical and serological status

2018 ◽  
Vol 9 (5) ◽  
pp. 1212-1220 ◽  
Author(s):  
Giuseppe Manna ◽  
Antonella Cersini ◽  
Roberto Nardini ◽  
Leticia Elisa Bartolomé Del Pino ◽  
Valeria Antognetti ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Southern Italy ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Preliminary Results ◽  
Serological Status

scholarly journals Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Runglawan Chawengkirttikul ◽  
Witchuta Junsiri ◽  
Amaya Watthanadirek ◽  
Napassorn Poolsawat ◽  
Sutthida Minsakorn ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Egg Production ◽  
Control Strategies ◽  
Amino Acid Sequences ◽  
Economic Losses ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Tropical Regions ◽  
Vaccination Programs ◽  
High Entropy

AbstractLeucocytozoon sabrazesi is the intracellular protozoa of leucocytozoonosis, which is transmitted by the insect vectors and affects chickens in most subtropical and tropical regions of the globe, except South America, and causing enormous economic losses due to decreasing meat yield and egg production. In this study, L. sabrazesi gametocytes have been observed in the blood smears, and molecular methods have been used to analyse the occurrence and genetic diversity of L. sabrazesi in blood samples from 313 chickens raised in northern, western and southern parts of Thailand. The nested polymerase chain reaction (nested PCR) assay based on the cytb gene revealed that 80.51% (252/313) chickens were positive of L. sabrazesi. The phylogenetic analysis indicated that L. sabrazesi cytb gene is conserved in Thailand, showed 2 clades and 2 subclades with similarity ranged from 89.5 to 100%. The diversity analysis showed 13 and 18 haplotypes of the sequences from Thailand and from other countries, respectively. The entropy analyses of nucleic acid sequences showed 26 high entropy peaks with values ranging from 0.24493 to 1.21056, while those of amino acid sequences exhibited 5 high entropy peaks with values ranging from 0.39267 to 0.97012. The results; therefore, indicate a high molecular occurrence of L. sabrazesi in chicken blood samples with the associated factors that is statistically significant (p < 0.05). Hence, our results could be used to improve the immunodiagnostic methods and to find appropriate preventive control strategies or vaccination programs against leucocytozoonosis in order to mitigate or eliminate the harmful impact of this infection on chicken industry.

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