Exemplar Abstract for Anaplasma platys Dumler et al. 2001.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Kara Mannor ◽  
George M Garrity
Keyword(s):  
Anaplasma Platys

scholarly journals Molecular detection of Anaplasma platys infection in free-roaming dogs and ticks from Kenya and Ivory Coast

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Ioana Adriana Matei ◽  
Gianluca D’Amico ◽  
Patrick K. Yao ◽  
Angela Monica Ionică ◽  
Paul W. N. Kanyari ◽  
...  
Keyword(s):  
Ivory Coast ◽  
Molecular Detection ◽  
Anaplasma Platys

scholarly journals Efeitos de diferentes doses de hiclato de doxiciclina nos parâmetros hematológicos de cães com erliquiose

2016 ◽  
Vol 37 (1) ◽  
pp. 53
Author(s):  
Mariana Cristina Hoeppner Rondelli ◽  
Reinaldo Juan Garrido Palacios Junior ◽  
Igor Luiz Salardani Senhorello ◽  
Áureo Evangelista Santana ◽  
Mirela Tinucci-Costa
Keyword(s):  
Nested Pcr ◽  
Ehrlichia Canis ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Leptospira Spp

Resumo: Este estudo objetivou comparar os efeitos de duas doses de hiclato de doxiciclina sobre a contagem de hemácias, concentração de hemoglobina, hematócrito, contagem de leucócitos e plaquetas de cães com erliquiose. Foram estudados os grupos I, composto por cães saudáveis (n=6), negativos na sorologia para Ehrlichia canis e Leptospira spp., na PCR em tempo real para E. canis e Anaplasma platys e na semi–nested PCR para Babesia canis; grupos II (n=6) e III (n=6), compostos por cães com história clínica sugestiva, sorologia e/ou PCR em tempo real positivas para E. canis, e negativos na pesquisa de anticorpos anti-Leptospira spp., na PCR em tempo real para A. platys e na semi–nested PCR para B. canis. Os cães doentes foram tratados com hiclato de doxiciclina via oral a cada 12 horas por 30 dias (5 mg/kg, grupo II; 10 mg/kg, grupo III). Hemogramas foram feitos antes, decorridos 15 dias e aos 10 dias após o término dos tratamentos. Não houve diferenças entre os grupos nos tempos avaliados quanto às variáveis hemácias, hemoglobina, hematócrito e leucócitos. Observou-se diferença quanto aos trombócitos entre o grupo I e os grupos II e III (p<0,0001) no início do estudo. Aos 15 dias de tratamento, a média do grupo III foi menor que dos grupos I (p=0,008) e II (p=0,0007), indicando persistência da trombocitopenia, ausente no grupo II. Não houve diferença entre os grupos no tempo final, sugerindo que ambos os tratamentos promoveram aumento da contagem de plaquetas em cães naturalmente infectados por E. canis.

scholarly journals First Molecular Detection of Anaplasma platys and Coinfection with Babesia gibsoni in Dogs from Bengaluru, India

2020 ◽  
Vol 9 (3) ◽  
pp. 75-79
Author(s):  
Medha Karnik ◽  
Anjan Kumar ◽  
M. Manjula ◽  
H. D. Lohitha ◽  
R. Narendra ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Anaplasma Platys ◽  
Babesia Gibsoni

scholarly journals First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand

2021 ◽  
pp. 2613-2619
Author(s):  
Biethee Rani Sarker ◽  
Thongphet Mitpasa ◽  
Arayaporn Macotpet ◽  
Pattara-Anong Bupata ◽  
Somboon Sangmaneedet ◽  
...  
Keyword(s):  
Risk Factors ◽  
Platelet Count ◽  
Dna Sequencing ◽  
Similarity Index ◽  
Rhipicephalus Sanguineus ◽  
Severe Thrombocytopenia ◽  
Anaplasma Platys ◽  
Related Factors ◽  
Nested Polymerase Chain Reaction ◽  
Khon Kaen

Background and Aim: Anaplasma platys is a blood parasite that infects platelets, causing thrombocytopenia. Rhipicephalus sanguineus ticks are believed to transmit A. platys. To identify A. platys, nested polymerase chain reaction (PCR) has proven to be an effective diagnostic tool. In this study, the molecular prevalence of A. platys infection in dogs was investigated for the 1st time in the Khon Kaen region of Thailand. The association between risk factors and A. platys infection was also evaluated. Materials and Methods: A total of 130 blood samples were collected from dogs in Khon Kaen, Thailand. DNA from the samples was extracted and nested PCR was applied for molecular analysis. Platelet count and packed cell volume (PCV) levels were measured. Platelet counts were categorized into four grades: Non-thrombocytopenia (platelets >200,000 cells/μL), mild thrombocytopenia (platelets 150,000-200,000 cells/μL), moderate thrombocytopenia (platelets 100,000-150,000 cells/μL), and severe thrombocytopenia (platelets <100,000 cells/μL). Four categories for PCV levels of >37%, 30-37%, 20-29%, and <20% were defined as no anemia, mild anemia, moderate anemia, and severe anemia, respectively. DNA sequencing was analyzed using BTSeq™ (Barcode-Tagged Sequencing; CELEMICS, Seoul, South Korea) for similarity index. Results: Among the 130 samples, 9 (6.9%) were positive for A. platys infection. There was an association between low platelet count and infection (p<0.05). PCV level was also associated with A. platys infection (p<0.05). DNA sequencing results of the nine positive samples showed similarity to known sequences of A. platys with 99.36-100% nucleotide identity. These results suggested low genetic diversity in A. platys infecting dogs in the Khon Kaen area. Conclusion: By amplifying 16S rRNA, A. platys infection was detected in the blood of Thai dogs. Further work should be performed to identify risk factors potentially associated with A. platys infection in dogs in Khon Kaen. Other related factors should also be considered, such as location and breeding, as well as the environmental characteristics of each locality. In addition, sampling a larger number of animals may reveal predictors for the positivity of A. platys in dogs in this region.

scholarly journals INCLUSÕES PLAQUETÁRIAS SEMELHANTES A Anaplasma platys (Ehrlichia platys) EM GATO

2005 ◽  
Vol 1 (2) ◽  
pp. 60-66 ◽  
Author(s):  
Vamilton Alvares Santarém ◽  
Cecília Braga Laposy ◽  
Marconi Rodrigues de Farias
Keyword(s):  
Anaplasma Platys

scholarly journals Serologic and Molecular Diagnosis of Anaplasma platys and Ehrlichia canis Infection in Dogs in an Endemic Region

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 488
Author(s):  
Bianca Lara ◽  
Anne Conan ◽  
Mary Anna Thrall ◽  
Jennifer K. Ketzis ◽  
Gillian Carmichael Branford ◽  
...  
Keyword(s):  
Early Stage ◽  
Point Of Care ◽  
False Negative ◽  
Ehrlichia Canis ◽  
Test Results ◽  
Anaplasma Platys ◽  
Endemic Region ◽  
Life Threatening ◽  
Improve Patient Management ◽  
Antibody Levels

Anaplasma platys and Ehrlichia canis are obligate intracellular, tick-borne rickettsial pathogens of dogs that may cause life-threatening diseases. In this study, we assessed the usefulness of PCR and a widely used commercial antibody-based point-of-care (POC) test to diagnose A. platys and E. canis infection and updated the prevalence of these pathogens in dogs inhabiting the Caribbean island of Saint Kitts. We detected A. platys in 62/227 (27%), E. canis in 84/227 (37%), and the presence of both in 43/227 (19%) of the dogs using PCR. POC testing was positive for A. platys in 53/187 (28%), E. canis in 112/187 (60%), and for both in 42/187 (22%) of the samples tested. There was only a slight agreement between A. platys PCR and POC test results and a fair agreement for E. canis PCR and POC test results. Our study suggests that PCR testing may be particularly useful in the early stage of infection when antibody levels are low or undetectable, whereas, POC test is useful when false-negative PCR results occur due to low bacteremia. A combination of PCR and POC tests may increase the ability to diagnose A. platys and E. canis infection and consequently will improve patient management.

Molecular detection of Rickettsia spp., Anaplasma platys and Theileria equi in ticks collected from horses in Tayrona National Park, Colombia

2019 ◽  
Vol 77 (3) ◽  
pp. 411-423
Author(s):  
Adriana Santodomingo ◽  
Keyla Sierra-Orozco ◽  
Andrea Cotes-Perdomo ◽  
Lyda R. Castro
Keyword(s):  
National Park ◽  
Molecular Detection ◽  
Anaplasma Platys ◽  
Theileria Equi

scholarly journals Ehrlichia canis e Anaplasma platys em carrapatos de cães de Cuiabá, Mato Grosso

2012 ◽  
Vol 33 (3) ◽  
pp. 1123-1126 ◽  
Author(s):  
Arleana do Bom P F de Almeida ◽  
Daphine Ariadne Jesus de Paula ◽  
Magyda Arabia A Dahroug ◽  
Agradia Goncalves de Freitas ◽  
Jose Nivaldo da Silva ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Mato Grosso

scholarly journals Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

2012 ◽  
Vol 21 (4) ◽  
pp. 379-385 ◽  
Author(s):  
Gislaine Cristina Ferreira da Silva ◽  
Aline do Nascimento Benitez ◽  
Aline Girotto ◽  
Alessandra Taroda ◽  
Marilda Carlos Vidotto ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Differential Diagnoses ◽  
Laboratory Evaluation ◽  
Blood Collection ◽  
Routine Laboratory ◽  
Anaplasma Platys ◽  
Pcr Assay ◽  
Blood Samples ◽  
Canine Monocytic Ehrlichiosis ◽  
Hematological Alterations

Canine monocytic ehrlichiosis caused primarily by Ehrlichia canis and canine thrombocytic anaplasmosis induced by Anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. There is evidence that these pathogens can also affect humans. This study evaluated the presence of E. canis and A. platys in blood samples collected from 256 domiciled dogs in the municipality of Jataizinho, located in north region of the State of Parana, Brazil, by PCR assay. The occurrence of E. canis and A. platys was 16.4% (42/256) and 19.4% (49/256), respectively; while 5.47% (14/256) of the dogs evaluated were co-infected by these two organisms. The presence of E. canis and A. platys was not significantly associated with the variables evaluated (sex, age, outdoor access, and presence of ticks during blood collection). Infection of dogs by E. canis was associated with anemia and thrombocytopenia, while infection induced by A. platys was related only to thrombocytopenia. Canine monocytic ehrlichiosis and canine thrombocytic anaplasmosis should be included in the differential diagnoses when these hematological alterations are observed during routine laboratory evaluation of dogs.

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