Multiple Resistance to Herbicides from Four Site-of-Action Groups in Waterhemp (Amaranthus tuberculatus)

Weed Science ◽  
2013 ◽  
Vol 61 (3) ◽  
pp. 460-468 ◽  
Author(s):  
Michael S. Bell ◽  
Aaron G. Hager ◽  
Patrick J. Tranel
Keyword(s):  
Field Experiments ◽  
Acetolactate Synthase ◽  
Glyphosate Resistance ◽  
The United States ◽  
Multiple Resistance ◽  
Site Mutation ◽  
High Frequencies ◽  
Site Of Action ◽  
Resistance To Herbicides ◽  
Action Groups

In 2006 and 2007, farmers from two counties in Illinois reported failure to control waterhemp with glyphosate. Subsequent onsite field experiments revealed that the populations might be resistant to multiple herbicides. Greenhouse experiments therefore were conducted to confirm glyphosate resistance, and to test for multiple resistance to other herbicides, including atrazine, acifluorfen, lactofen, and imazamox. In glyphosate dose-response experiments, both populations responded similarly to a previously characterized glyphosate-resistant population (MO1). Both Illinois populations also demonstrated high frequencies of resistance to the acetolactate synthase (ALS) inhibitor, imazamox. Additionally, one of the populations demonstrated high frequencies of resistance to both atrazine and the protoporphyrinogen oxidase (PPO) inhibitor, lactofen. Furthermore, using combinations of sequential and tank-mix herbicide applications, individual plants resistant to herbicides spanning all four site-of-action groups were identified from one population. Molecular experiments were performed to provide an initial characterization of the resistance mechanisms and to provide confirmation of the presence of multiple resistance traits within the two populations. Both populations contained the W574L ALS mutation and the ΔG210 PPO mutation, previously shown to confer resistance to ALS and PPO inhibitors, respectively. Atrazine resistance in both populations is suspected to be metabolism-based, because a triazine target-site mutation was not identified. A P106S EPSPS mutation, previously reported to confer glyphosate resistance, was identified in one population. This mutation was identified in both resistant and sensitive plants from the population; however, and so more research is needed to determine the glyphosate-resistance mechanism(s). This is the first known case of a weed population in the United States possessing multiple resistance to herbicides from four site-of-action groups.

Glyphosate and Multiple Herbicide Resistance in Common Waterhemp (Amaranthus rudis)Populations from Missouri

Weed Science ◽  
2008 ◽  
Vol 56 (4) ◽  
pp. 582-587 ◽  
Author(s):  
Travis R. Legleiter ◽  
Kevin W. Bradley
Keyword(s):  
Field Experiments ◽  
Acetolactate Synthase ◽  
The United States ◽  
Multiple Resistance ◽  
Complete Control ◽  
Individual Female ◽  
Common Waterhemp ◽  
Greenhouse Experiments ◽  
Treatment Tank ◽  
Amaranthus Rudis

Field and greenhouse experiments were conducted to determine the level of glyphosate resistance in common waterhemp populations from Platte County (MO1) and Holt County, Missouri (MO2), and to determine the level and distribution of resistance to glyphosate, acetolactate synthase (ALS)–inhibiting herbicides, and protoporophyrinogen oxidase (PPO)–inhibiting herbicides across the MO1 site. Results from greenhouse experiments revealed that the MO1 and MO2 waterhemp populations were 19 and 9 times more resistant to glyphosate, respectively, than a susceptible waterhemp population. In field experiments, greater than 54% of waterhemp at the MO1 site survived 1.7 kg glyphosate ae ha−1(twice the labeled rate) 6 wk after treatment. Tank-mix combinations of ALS- and PPO-inhibiting herbicides with glyphosate also failed to provide complete control of the waterhemp population at the MO1 site. Collection and screening of seed from individual female waterhemp accessions revealed multiple resistance to glyphosate, ALS-, and PPO-inhibiting herbicides across the MO1 site. All 14 waterhemp accessions collected across the MO1 site exhibited greater than 65% survival to 2× rates of glyphosate and thifensulfuron, and these accessions were spread across a 5-km2(503-ha) area. Four waterhemp accessions collected across a 0.9-km2(87-ha) area also exhibited 26 to 38% survival to 2× rates of lactofen. The results from these experiments provide evidence and confirmation of the first glyphosate-resistant waterhemp population in the United States and reveal that multiple resistance to glyphosate, ALS-, and PPO-inhibiting herbicides can occur in waterhemp.

scholarly journals Wild oat (Avena fatua L.) biotypes resistant to acetolactate synthase and acetyl-CoA carboxylase inhibitors in Poland  

2013 ◽  
Vol 59 (No. 9) ◽  
pp. 432-437 ◽  
Author(s):  
K. Adamczewski ◽  
R. Kierzek ◽  
K. Matysiak
Keyword(s):  
Resistance Index ◽  
Acetolactate Synthase ◽  
Avena Fatua ◽  
Multiple Resistance ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Site Mutation ◽  
Metabolic Resistance ◽  
Mechanism Of Resistance ◽  
And Control

The aim of the study was to collect seeds of wild oat from the fields where, in spite of the applied herbicides, the weed is very poorly controlled, and to determine under greenhouse conditions if any resistant biotypes are present. In the years 2008–2011, 34 samples of wild oat were collected from fields where the weed was poorly controlled. The biotypes were analyzed in greenhouse experiments to determine if they are resistant to herbicides. Among five resistant biotypes three of them (R3, R4 and R5) were resistant only to iodosulfuron and mesosulfuron, and biotype R2 – only to propoxycarbazone-sodium. Biotype R1 exhibited multiple resistance to iodosulfuron + mesosulfuron and pinoxaden. The use of sulfometuron proves that the mechanism of resistance of two biotypes of wild oat (R1 and R4) to acetolactate synthase inhibitors is associated with target-site mutation. The curve of biotypes R3 and R5 controlled with iodosulfuron + mesosulfuron shows a relatively low resistance index and control of those biotypes with sulfometuron indicates a metabolic resistance.

Heritability of Glyphosate Resistance in Indiana Horseweed (Conyza canadensis) Populations

Weed Science ◽  
2010 ◽  
Vol 58 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Vince M. Davis ◽  
Greg R. Kruger ◽  
Steven G. Hallett ◽  
Patrick J. Tranel ◽  
William G. Johnson
Keyword(s):  
First Generation ◽  
Dominant Gene ◽  
Mail Survey ◽  
Acetolactate Synthase ◽  
Glyphosate Resistance ◽  
The United States ◽  
Cotton Production ◽  
Low Frequencies ◽  
Generation Progeny ◽  
High Level

Horseweed has rapidly become a major weed in soybean and cotton production fields of the United States, and Indiana farmers ranked horseweed as one of the five worst weeds in their fields during a mail survey in 2003. Glyphosate resistance in horseweed is conferred by a single, incompletely dominant gene. Horseweed populations possess a high level of variability in their response to glyphosate. Horseweed has also evolved resistance to acetolactate synthase (ALS) inhibitors, and biotypes resistant to ALS-inhibiting herbicides and glyphosate are in many of the same areas. An experiment was designed to determine whether glyphosate resistance can be transferred by pollen. We found glyphosate-resistant plants in 1.1 to 3.8% of the progeny. Segregation ratios fit the expected 3 : 1 resistant : sensitive ratios confirming that glyphosate resistance in horseweed can transfer to closely located glyphosate-susceptible biotypes under open-pollinated conditions at low frequencies. The hypothesis of a follow-up experiment was that first-generation progeny of parent plants, selected on a continuum of low to high phenotypic response to glyphosate, will inherit respective low to high phenotypic responses to glyphosate. The variability in field-collected populations (low-level to high-level glyphosate resistance) ranged from 2 to 14 times the commonly recommended field use rate of glyphosate. However, low- and high-levels of glyphosate resistance were not observed in first-generation progeny. We conclude that differential glyphosate responses observed among parental populations was due to different frequencies of the resistance allele within the populations, rather than the presence of different resistance alleles.

scholarly journals INHERITANCE OF RESISTANCE TO PROTOPORPHYRINOGEN OXIDASE INHIBITOR HERBICIDES IN WILD POINSETTIA

2016 ◽  
Vol 34 (3) ◽  
pp. 575-580 ◽  
Author(s):  
A.P. BRUSAMARELLO ◽  
P.H. OLIVEIRA ◽  
M.M. TREZZI ◽  
E. XAVIER ◽  
E.D. DALOSTO
Keyword(s):  
Single Gene ◽  
Nuclear Gene ◽  
Female Parent ◽  
Acetolactate Synthase ◽  
Oxidase Inhibitor ◽  
Multiple Resistance ◽  
Protoporphyrinogen Oxidase ◽  
First Case ◽  
Resistance To Herbicides ◽  
F1 Generation

ABSTRACT Weed resistance to herbicides is a major global problem for agriculture. In recent years, the increased use of herbicides, without proper planning, has led to a large increase in the number of cases of weed biotypes that are resistant to one or more herbicide mechanism of action. Wild poinsettia biotypes (Euphorbia heterophylla), discovered in the State of Paraná, with resistance to herbicides that inhibit protoporphyrinogen oxidase (PROTOX) and acetolactate synthase (ALS), are the first case to exhibit multiple resistance in Brazil. This study analyzed the genetic inheritance of PROTOX inhibiting herbicide resistance in E. heterophylla. Crosses were conducted between biotypes that were susceptible (female parent) and those carrying multiple resistance to ALS and PROTOX inhibitors (male parent) to obtain the F1 generation. Backcrosses were performed (RC1 and RC2) and the F1 generation was advanced to F2. F1, F2, RC1, RC2 generation plants and their parents were subjected to applications of the herbicide fomesafen (250 g i.a. ha-1). The observed frequencies in the F2 generation were not different from the expected frequency of 3:1. All RC2 individuals were resistant, while the RC1 population showed a 1:1 segregation, which would be expected when a single gene controls the trait. Thus, it can be inferred that E. heterophylla resistance to PROTOX-inhibiting herbicides is conditioned by a single and dominant nuclear gene.

Characterization of a waterhemp (Amaranthus tuberculatus) population from Illinois resistant to herbicides from five site-of-action groups

2019 ◽  
Vol 33 (03) ◽  
pp. 400-410 ◽  
Author(s):  
Cody M. Evans ◽  
Seth A. Strom ◽  
Dean E. Riechers ◽  
Adam S. Davis ◽  
Patrick J. Tranel ◽  
...  
Keyword(s):  
Field Experiments ◽  
Acetolactate Synthase ◽  
Resistance Mechanisms ◽  
Synthetic Auxin ◽  
Amaranthus Tuberculatus ◽  
Resistance To Herbicides ◽  
Central Illinois ◽  
Sites Of Action ◽  
Herbicide Use

AbstractExperiments were initiated to characterize a waterhemp population (CHR) discovered in a central Illinois corn field after it was not controlled by the 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor topramezone. Field experiments conducted during 2014–2015 indicated that acetolactate synthase (ALS)-, protoporphyrinogen oxidase (PPO)-, photosystem II (PSII)-, and HPPD-inhibiting herbicides and the synthetic auxin 2,4-D did not control the CHR population. Laboratory experiments confirmed target site–based resistance mechanisms to ALS- and PPO-inhibiting herbicides. Herbicide doses required to reduce dry biomass 50% (GR50) were determined in greenhouse dose–response experiments, and indicated 16-fold resistance to the HPPD inhibitor mesotrione, 9.5-fold resistance to the synthetic auxin 2,4-D, and 252-fold resistance to the PSII inhibitor atrazine. Complementary results from field, laboratory, and greenhouse investigations indicate that the CHR population has evolved resistance to herbicides from five sites of action (SOAs): ALS-, PPO-, PSII-, and HPPD-inhibiting herbicides and 2,4-D. Herbicide use history for the field in which CHR was discovered indicates no previous use of 2,4-D.

Interspecific Hybridization: Potential for Movement of Herbicide Resistance from Wheat to Jointed Goatgrass (Aegilops cylindrica)

2005 ◽  
Vol 19 (3) ◽  
pp. 674-682 ◽  
Author(s):  
Bradley D. Hanson ◽  
Carol A. Mallory-Smith ◽  
William J. Price ◽  
Bahman Shafii ◽  
Donald C. Thill ◽  
...  
Keyword(s):  
Pacific Northwest ◽  
Great Plains ◽  
Herbicide Resistance ◽  
Field Experiments ◽  
Introgressive Hybridization ◽  
Acetolactate Synthase ◽  
The United States ◽  
Aegilops Cylindrica ◽  
Herbicide Resistant ◽  
Jointed Goatgrass

The transfer of herbicide resistance genes from crops to related species is one of the greatest risks of growing herbicide-resistant crops. The recent introductions of imidazolinone-resistant wheat in the Great Plains and Pacific Northwest regions of the United States and research on transgenic glyphosate-resistant wheat have raised concerns about the transfer of herbicide resistance from wheat to jointed goatgrass via introgressive hybridization. Field experiments were conducted from 2000 to 2003 at three locations in Washington and Idaho to determine the frequency and distance that imidazolinone-resistant wheat can pollinate jointed goatgrass and produce resistant F1hybrids. Each experiment was designed as a Nelder wheel with 16 equally spaced rays extending away from a central pollen source of ‘Fidel-FS4’ imidazolinone-resistant wheat. Each ray was 46 m long and contained three rows of jointed goatgrass. Spikelets were collected at maturity at 1.8-m intervals along each ray and subjected to an imazamox screening test. The majority of all jointed goatgrass seeds tested were not resistant to imazamox; however, 5 and 15 resistant hybrids were found at the Pullman, WA, and Lewiston, ID, locations, respectively. The resistant plants were identified at a maximum distance of 40.2 m from the pollen source. The overall frequency of imazamox-resistant hybrids was similar to the predicted frequency of naturally occurring acetolactate synthase resistance in weeds; however, traits with a lower frequency of spontaneous mutations may have a relatively greater risk for gene escape via introgressive hybridization.

scholarly journals Responses of a Waterhemp (Amaranthus tuberculatus) Population Resistant to HPPD-Inhibiting Herbicides to Foliar-Applied Herbicides

2016 ◽  
Vol 30 (1) ◽  
pp. 106-115 ◽  
Author(s):  
Nicholas E. Hausman ◽  
Patrick J. Tranel ◽  
Dean E. Riechers ◽  
Aaron G. Hager
Keyword(s):  
Photosystem Ii ◽  
Field Data ◽  
Growth Stage ◽  
Acetolactate Synthase ◽  
Herbicide Application ◽  
Greenhouse Experiments ◽  
County Population ◽  
Site Of Action ◽  
Amaranthus Tuberculatus ◽  
Action Groups

Field and greenhouse experiments were conducted to characterize the response of a waterhemp population from McLean County, IL to foliar-applied 4-hydroxyphenylpyruvate dioxygenase (HPPD) –inhibiting herbicides and determine the population's sensitivity to herbicides from other site-of-action groups. In the field, 10 to 15–cm-tall waterhemp treated with mesotrione at 105 g ai ha−1, tembotrione at 92 g ai ha−1, or topromezone at 18 g ai ha−1had significantly greater biomass (≥ 10%) 14 d after treatment (DAT) than waterhemp harvested the day of herbicide application, indicating growth had occurred following herbicide application. Waterhemp growth stage at the time of herbicide application influenced control. Mesotrione applied at 105 g ha−1alone or combined with atrazine at 560 g ai ha−1provided significantly greater waterhemp control (≥ 66%) when applied to small waterhemp plants (2 to 5 cm tall) compared with applications made to plants 5 to 10 or 10 to 15 cm tall. Glyphosate, glufosinate, fomesafen, lactofen, or acifluorfen provided greater waterhemp control (≥ 68%) 7 and 14 DAT than mesotrione, dicamba, or 2,4-D. Control of this population with atrazine, chlorimuron, and imazethapyr did not exceed 12%. Results of a greenhouse experiment with waterhemp plants grown from field-collected seed were similar to field data, and confirm the McLean County population was poorly controlled with HPPD, photosystem II, and acetolactate synthase inhibitors.

scholarly journals Distribution of Herbicide Resistances and Molecular Mechanisms Conferring Resistance in Missouri Waterhemp (Amaranthus rudisSauer) Populations

Weed Science ◽  
2015 ◽  
Vol 63 (1) ◽  
pp. 336-345 ◽  
Author(s):  
John L. Schultz ◽  
Laura A. Chatham ◽  
Chance W. Riggins ◽  
Patrick J. Tranel ◽  
Kevin W. Bradley
Keyword(s):  
Amino Acid ◽  
Amino Acid Substitution ◽  
Molecular Mechanisms ◽  
Dna Analysis ◽  
D1 Protein ◽  
Acetolactate Synthase ◽  
Glyphosate Resistance ◽  
Mechanisms Of Action ◽  
Resistance To Herbicides ◽  
Inhibitor Resistance

A survey of soybean fields containing waterhemp was conducted just prior to harvest in 2012 to determine the scope and extent of herbicide resistance and multiple herbicide resistances among a sample of Missouri waterhemp populations. Resistance was confirmed to glyphosate and to acetolactate synthase (ALS), protoporphyrinogen oxidase (PPO), photosystem II (PSII), and 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors, but not to 2,4-D. Of the 187 populations tested, 186 exhibited resistance to chlorimuron. The proportions of populations with atrazine or glyphosate resistance were similar, with 30 and 29% of the populations surviving the 3× rates. Lactofen resistance was observed in 5% of the populations, whereas mesotrione resistance was only found in 1.6% of the populations. All populations tested were susceptible to 2,4-D at the 3× rate. At least 52% of the waterhemp populations tested exhibited resistance to herbicides from two mechanism of action. Resistance to atrazine plus chlorimuron as well as glyphosate plus chlorimuron was present in 29% of the populations. Three-way resistance, primarily comprised of resistance to atrazine plus chlorimuron plus glyphosate, was present in 11% of the populations. Resistance to herbicides from four mechanisms of action was found in 2% of the populations, and one population exhibited resistance to herbicides from five mechanisms of action. DNA analysis of a subsample of plants revealed that previously documented mechanisms of resistance in waterhemp, including the ΔG210 deletion conferring PPO-inhibitor resistance, the Trp574Leu amino acid substitution conferring ALS-inhibitor resistance, and elevated 5-enolypyruvyl-shikimate-3-phosphate synthase copy number and the Pro106Ser amino acid substitution resulting in glyphosate resistance, explained survival in many, but not all, instances. Atrazine resistance was not explained by the Ser264Gly D1 protein substitution. Overall, results from these experiments indicate that Missouri soybean fields contain waterhemp populations with resistance to glyphosate, ALS-, PPO-, PSII-, and HPPD-inhibiting herbicides, which are some of the most common mechanisms of action currently utilized for the control of this species in corn and soybean production systems. Additionally, these results indicate that slightly more than half of the populations tested exhibit resistance to more than one herbicide mechanisms of action. Managing the current resistance levels in existing populations is of utmost importance. The use of multiple, effective herbicide modes of action, both preemergence and postemergence, and the integration of optimum cultural and mechanical control practices will be vital in the management of Missouri waterhemp populations in the future.

Molecular basis of multiple resistance to herbicides inhibiting acetyl-CoA carboxylase and acetolactate synthase in American sloughgrass (Beckmannia syzigachne) from China

2016 ◽  
Vol 67 (11) ◽  
pp. 1208 ◽  
Author(s):  
Lang Pan ◽  
Haitao Gao ◽  
Han Wu ◽  
Liyao Dong
Keyword(s):  
Acetolactate Synthase ◽  
Alternative Methods ◽  
Multiple Resistance ◽  
Homozygous Mutation ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Wheat Field ◽  
Sequence Method ◽  
Resistance To Herbicides ◽  
Als Inhibitors

American sloughgrass (Beckmannia syzigachne Steud.) is a problematic grass that is widely distributed in wheat and oilseed rape fields in China. The herbicides fenoxaprop-P-ethyl and mesosulfuron-methyl failed to control B. syzigachne JCWJ-R populations collected from a wheat field in Jiangsu Province. Dose-response experiments showed that JCWJ-R was resistant to the acetyl-CoA carboxylase (ACCase) inhibitors fenoxaprop-P-ethyl (33.8-fold), haloxyfop-R-methyl (12.7-fold), clethodim (7.8-fold) and pinoxaden (11.6-fold), and to the acetolactate synthase (ALS) inhibitors mesosulfuron-methyl (15.9-fold), pyroxsulam (17.6-fold), flucarbazone-Na (10.7-fold) and imazethapyr (7-fold). Resistance to ALS inhibitors was due to a Pro-197-Ser mutation in the ALS gene and resistance to ACCase inhibitors was due to an Ile-1781-Leu mutation in the ACCase gene. A derived cleaved amplified polymorphic sequence method was developed to detect the ALS mutation in B. syzigachne. This was combined with a previously established method to detect Ile-1781-Leu, and the mutation frequency and homozygous mutation rates in the JCWJ-R population were determined. The evolution of multiple resistance to ACCase and ALS inhibitors in this B. syzigachne population indicated that alternative methods should be developed to control resistant weeds.

A Survey for Diclofop-Methyl Resistance in Italian Ryegrass from Tennessee and How To Manage Resistance in Wheat

2010 ◽  
Vol 24 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Andrew T. Ellis ◽  
Lawrence E. Steckel ◽  
Christopher L. Main ◽  
Marcel S. C. De Melo ◽  
Dennis R. West ◽  
...  
Keyword(s):  
Field Experiments ◽  
Wheat Yield ◽  
Acetolactate Synthase ◽  
The United States ◽  
Research Field ◽  
Italian Ryegrass ◽  
Cross Resistance ◽  
Susceptible Population ◽  
Application Timing ◽  
Herbicide Programs

Italian ryegrass resistance to diclofop has been documented in several countries, including the United States. The purpose of this research was to screen selected putative resistant populations of Italian ryegrass for resistance to the acetyl-CoA carboxylase (ACCase)–inhibiting herbicides diclofop and pinoxaden and the acetolactate synthase (ALS)–inhibiting herbicides imazamox, pyroxsulam, and mesosulfuron in the greenhouse and to use field experiments to develop herbicide programs for Italian ryegrass control. Resistance to diclofop was confirmed in eight populations from Tennessee. These eight populations did not show cross-resistance to pinoxaden. One additional population (R1) from Union County, North Carolina, was found to be resistant to both diclofop and pinoxaden. The level of resistance to pinoxaden of the R1 population was 15 times that of the susceptible population. No resistance was confirmed to any of the ALS-inhibiting herbicides examined in this research. Field experiments demonstrated PRE Italian ryegrass control with chlorsulfuron (71 to 94%) and flufenacet + metribuzin (84 to 96%). Italian ryegrass control with pendimethalin applied PRE or delayed preemergence (DPRE) was variable (0 to 85%). POST control of Italian ryegrass was acceptable with pinoxaden, mesosulfuron, flufenacet + metribuzin, and chlorsulfuron + flucarbazone (> 80%). Application timing and herbicide treatment had no effect on wheat yield, except for diclofop and pendimethalin treatments, in which uncontrolled Italian ryegrass reduced wheat yield.

Sign in / Sign up

Export Citation Format

Share Document