Heritability of Glyphosate Resistance in Indiana Horseweed (Conyza canadensis) Populations

Weed Science ◽  
2010 ◽  
Vol 58 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Vince M. Davis ◽  
Greg R. Kruger ◽  
Steven G. Hallett ◽  
Patrick J. Tranel ◽  
William G. Johnson

Horseweed has rapidly become a major weed in soybean and cotton production fields of the United States, and Indiana farmers ranked horseweed as one of the five worst weeds in their fields during a mail survey in 2003. Glyphosate resistance in horseweed is conferred by a single, incompletely dominant gene. Horseweed populations possess a high level of variability in their response to glyphosate. Horseweed has also evolved resistance to acetolactate synthase (ALS) inhibitors, and biotypes resistant to ALS-inhibiting herbicides and glyphosate are in many of the same areas. An experiment was designed to determine whether glyphosate resistance can be transferred by pollen. We found glyphosate-resistant plants in 1.1 to 3.8% of the progeny. Segregation ratios fit the expected 3 : 1 resistant : sensitive ratios confirming that glyphosate resistance in horseweed can transfer to closely located glyphosate-susceptible biotypes under open-pollinated conditions at low frequencies. The hypothesis of a follow-up experiment was that first-generation progeny of parent plants, selected on a continuum of low to high phenotypic response to glyphosate, will inherit respective low to high phenotypic responses to glyphosate. The variability in field-collected populations (low-level to high-level glyphosate resistance) ranged from 2 to 14 times the commonly recommended field use rate of glyphosate. However, low- and high-levels of glyphosate resistance were not observed in first-generation progeny. We conclude that differential glyphosate responses observed among parental populations was due to different frequencies of the resistance allele within the populations, rather than the presence of different resistance alleles.

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1073D-1073
Author(s):  
Richard L. Fery ◽  
Judy A. Thies

The USDA–ARS has released a new Habanero-type pepper cultivar named TigerPaw-NR. The new cultivar is the product of a conventional recurrent backcross breeding procedure to transfer a dominant root-knot nematode resistance gene from the Scotch Bonnet accession PA-426 into the Habanero-type accession PA-350. TigerPaw-NR was derived from a single F3BC4 plant grown in 2002. TigerPaw-NR is homozygous for a dominant gene conditioning a high level of resistance to the southern root-knot nematode, the peanut root-knot nematode, and the tropical root-knot nematode. TigerPaw-NR has a compact plant habit and produces attractive lantern-shaped, orange-colored fruit. The results of three replicated field studies conducted at Charleston, S.C., indicate that the fruit and yield characteristics of TigerPaw-NR are comparable to those of currently available Habanero-type cultivars. A typical fruit weighs 7.8 g, is 2.7 cm wide × 4.4 cm long, and is extremely pungent (348,634 Scoville heat units). Root-knot nematodes are major pests of peppers in the United States, and all Habanero-type cultivars currently available to commercial growers and home gardeners are susceptible. The root-knot nematode resistant TigerPaw-NR is recommended for use by both commercial growers and home gardeners. Protection for TigerPaw-NR is being sought under the Plant Variety Protection Act.


Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1496
Author(s):  
Balaji Aravindhan Pandian ◽  
Abigail Friesen ◽  
Martin Laforest ◽  
Dallas E. Peterson ◽  
P. V. Vara Prasad ◽  
...  

Wild buckwheat (Polygonum convolvulus L.) is a problem weed and ALS-inhibitors (e.g., chlorsulfuron) are commonly used for its management. Recently, a population of wild buckwheat (KSW-R) uncontrolled with ALS-inhibitors was found in a wheat field in Kansas, USA. The objectives of this research were to determine the level and mechanism of resistance to chlorsulfuron and cross resistance to other ALS-inhibitors in the KSW-R population. In response to chlorsulfuron rates ranging from 0 to 16x (x = 18 g ai/ha), the KSW-R wild buckwheat was found >100-fold more resistant compared to a known ALS-inhibitor susceptible (KSW-S) wild buckwheat. Also, >90% of KSW-R plants survived field recommended rates of sulfonylurea but not imidazolinone family of ALS-inhibitors. A portion of the ALS gene covering all previously reported mutations known to bestow resistance to ALS-inhibitors was sequenced from both KSW-R and KSW-S plants. The Pro-197-Ser substitution that confers resistance to the sulfonylurea herbicides was found in KSW-R plants. Our results support the evolution of high level of chlorsulfuron resistance as a result of a mutation in the ALS-gene in KSW-R buckwheat. This is the first case of resistance to any herbicides in wild buckwheat in the US.


Weed Science ◽  
2013 ◽  
Vol 61 (3) ◽  
pp. 460-468 ◽  
Author(s):  
Michael S. Bell ◽  
Aaron G. Hager ◽  
Patrick J. Tranel

In 2006 and 2007, farmers from two counties in Illinois reported failure to control waterhemp with glyphosate. Subsequent onsite field experiments revealed that the populations might be resistant to multiple herbicides. Greenhouse experiments therefore were conducted to confirm glyphosate resistance, and to test for multiple resistance to other herbicides, including atrazine, acifluorfen, lactofen, and imazamox. In glyphosate dose-response experiments, both populations responded similarly to a previously characterized glyphosate-resistant population (MO1). Both Illinois populations also demonstrated high frequencies of resistance to the acetolactate synthase (ALS) inhibitor, imazamox. Additionally, one of the populations demonstrated high frequencies of resistance to both atrazine and the protoporphyrinogen oxidase (PPO) inhibitor, lactofen. Furthermore, using combinations of sequential and tank-mix herbicide applications, individual plants resistant to herbicides spanning all four site-of-action groups were identified from one population. Molecular experiments were performed to provide an initial characterization of the resistance mechanisms and to provide confirmation of the presence of multiple resistance traits within the two populations. Both populations contained the W574L ALS mutation and the ΔG210 PPO mutation, previously shown to confer resistance to ALS and PPO inhibitors, respectively. Atrazine resistance in both populations is suspected to be metabolism-based, because a triazine target-site mutation was not identified. A P106S EPSPS mutation, previously reported to confer glyphosate resistance, was identified in one population. This mutation was identified in both resistant and sensitive plants from the population; however, and so more research is needed to determine the glyphosate-resistance mechanism(s). This is the first known case of a weed population in the United States possessing multiple resistance to herbicides from four site-of-action groups.


1962 ◽  
Vol 16 (3) ◽  
pp. 665-666

The 21st plenary meeting of the International Cotton Advisory Committee (ICAC) took place in Washington from May 9 to May 23, 1962. In the Committee of the Whole, attention was given to the world supply and demand situation, government actions affecting cotton, and steps needed for the further advance of the world cotton economy. A statement, prepared by a working group headed by Mr. Edouard J. Senn (France), developed from the discussions of the committee, presented the following observations, among others: 1) The overall statistical situation in raw cotton remained strong. Acreage had risen to a new peak; cotton consumption was at a record high level; there had been a reasonable degree of price stability in world cotton markets; and most countries would probably dispose of the bulk of their exportable supplies. World stocks were likely to decline to their lowest level since 1953. The committee recognized, however, that the over-all reduction in cotton stocks in the period under review had been brought about mainly by reason of partial crop failures in certain countries and that, with normal yields in these countries, the relationship between supply and demand could well have been reversed. 2) Cotton consumption continued to break new records with each successive season, but undue optimism had to be tempered by an awareness of the intense competition from the manmade fibers which had brought about a decline in cotton's share of the total textile market. 3) The profit margins of cotton textile manufacturers, as well as of producers, were also limited by rising costs and by the highly competitive nature of the textile industry. 4) In the opinion of the committee there was no doubt that the restriction of acreage in the United States had been a major factor in the orderly liquidation of the cotton surplus and in the return to a better balance between world cotton supply and demand. 5) The committee noted with considerable interest the statement of the United States delegation that attention was currently being given to a number of plans to introduce a greater element of freedom in the manner of cotton production and to reduce or eliminate the differential between domestic and export prices in the United States. 6) The committee also welcomed the reassurance by the United States delegation that in formulating its cotton policy the United States would always have in mind the interests of other nations and seek to avoid any dislocation of the world cotton market.


2005 ◽  
Vol 49 (12) ◽  
pp. 5007-5012 ◽  
Author(s):  
Louis B. Rice ◽  
Lenore L. Carias ◽  
Susan Rudin ◽  
Viera Laktičová ◽  
Aaron Wood ◽  
...  

ABSTRACT Using 15 unrelated Enterococcus faecium isolates as donors, we demonstrated that ampicillin resistance was transferable to an E. faecium recipient containing a pbp5 deletion for all but four strains. The transfers occurred at low frequencies (generally ca. 10−9 transconjugants/recipient CFU), consistent with chromosome-to-chromosome transfer. pbp5 transfer occurred within large genetic regions, and insertion into the recipient genome occurred most commonly into the recipient SmaI restriction fragment that had been created by the previous pbp5 deletion. Restriction mapping of the region upstream of pbp5 revealed a commonality of fragment sizes among the clinical isolates from the United States which differed significantly from those of three strains that were isolated from turkey feces. These data prove conclusively that E. faecium pbp5 is a transferable determinant, even in the absence of a coresiding vancomycin resistance mobile element. They also suggest that the spread of high-level ampicillin resistance among U.S. E. faecium strains is due in part to the transfer of low-affinity pbp5 between clinical isolates.


Author(s):  
Leah Plunkett ◽  
Urs Gasser ◽  
Sandra Cortesi

New types of digital technologies and new ways of using them are heavily impacting young people’s learning environments and creating intense pressure points on the “pre-digital” framework of student privacy. This chapter offers a high-level mapping of the federal legal landscape in the United States created by the “big three” federal privacy statutes—the Family Educational Rights and Privacy Act (FERPA), the Children’s Online Privacy Protection Act (COPPA), and the Protection of Pupil Rights Amendment (PPRA)—in the context of student privacy and the ongoing digital transformation of formal learning environments (“schools”). Fissures are emerging around key student privacy issues such as: what are the key data privacy risk factors as digital technologies are adopted in learning environments; which decision makers are best positioned to determine whether, when, why, and with whom students’ data should be shared outside the school environment; what types of data may be unregulated by privacy law and what additional safeguards might be required; and what role privacy law and ethics serve as we seek to bolster related values, such as equity, agency, and autonomy, to support youth and their pathways. These and similar intersections at which the current federal legal framework is ambiguous or inadequate pose challenges for key stakeholders. This chapter proposes that a “blended” governance approach, which draws from technology-based, market-based, and human-centered privacy protection and empowerment mechanisms and seeks to bolster legal safeguards that need to be strengthen in parallel, offers an essential toolkit to find creative, nimble, and effective multistakeholder solutions.


2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 413-414
Author(s):  
Carlyn Vogel ◽  
Debra Dobbs ◽  
Brent Small

Abstract Spirituality is difficult to define as researchers assign it different meanings and individuals’ perceptions can vary. For example, spirituality may connect to religiosity, while others consider religiosity a less significant part of spirituality. This study investigates factors outside of religiosity that are significantly associated with spirituality to inform the characteristics of the concept. Webster’s (2004) existential framework of spirituality was used to guide variable selection. The National Survey of Midlife in the United States wave three (MIDUS 3; 2013-2014; n = 2,594; Mage = 63.5, SD = 11, range = 39–92) was used to examine individuals’ reported levels of spirituality. Multinomial logistic regression was conducted to examine factors related to low and high levels of spirituality compared to a moderate level. Participants with low spirituality were more likely to be male, less likely to be mindful, mediate/chant, feel a strong connection to all life, to indicate that they cannot make sense of the world, and to be religious. Participants with high spirituality were more likely to be female, have at least some college experience, be mindful, meditate/chant, feel deep inner peace, have a sense of deep appreciation, think that a sense of purpose is important for a good life, and have a high level of religiosity. Framed by Webster’s conceptual model, the current study observed that religiosity is significantly associated with spirituality and that other mindfulness-based aspects are also present within this concept. Incorporating mindfulness with religious efforts will more accurately and holistically address spirituality.


Author(s):  
Coby Klein ◽  
Mitchell Baker ◽  
Andrei Alyokhin ◽  
David Mota-Sanchez

Abstract Eastern New York State is frequently the site of Colorado potato beetle (Leptinotarsa decemlineata, Say) populations with the highest observed levels of insecticide resistance to a range of active ingredients. The dominance of a resistant phenotype will affect its rate of increase and the potential for management. On organic farms on Long Island, L. decemlineata evolved high levels of resistance to spinosad in a short period of time and that resistance has spread across the eastern part of the Island. Resistance has also emerged in other parts of the country as well. To clarify the level of dominance or recessiveness of spinosad resistance in different parts of the United States and how resistance differs in separate beetle populations, we sampled in 2010 beetle populations from Maine, Michigan, and Long Island. In addition, a highly resistant Long Island population was assessed in 2012. All populations were hybridized with a laboratory-susceptible strain to determine dominance. None of the populations sampled in 2010 were significantly different from additive resistance, but the Long Island population sampled in 2012 was not significantly different from fully recessive. Recessive inheritance of high-level resistance may help manage its increase.


2021 ◽  
Vol 13 (9) ◽  
pp. 4861
Author(s):  
Marcin Bogdański

Differentiated response of selected economies to the global economic crisis caused by the collapse of the real estate market in the United States has drawn the attention of economists to the concept of economic resilience. At the same time, once again, it showed the importance of analysing and creating suitable conditions for sustainable development. Resilient economies are less exposed to the risk of economic crises or slowdowns, which is vital for ensuring stable incomes and high level of living standards. Therefore, the presented analysis was aimed at evaluating the level of economic resilience of provincial cities in Poland in relation to the situation on their labour markets. For this purposes, selected measures of the variation in the distribution feature (e.g., coefficient of variation) and the degree of structure diversification of the examined feature (Amemiya’s index) were used. Subsequently, using correlation analysis, the research determined whether any relationships could be observed between the investigated variables. The results of the research indicate that for provincial cities sub-regions in Poland, a statistically significant, moderate negative correlation could be observed between the degree of employment structure diversification in 2009 and the scale and scope of the collapse in the number of employed persons in subsequent years. This suggests that a high level of employment diversification restricted the level of economic resilience in this case.


Weed Science ◽  
2021 ◽  
pp. 1-25
Author(s):  
Qian Yang ◽  
Xia Yang ◽  
Zichang Zhang ◽  
Jieping Wang ◽  
Weiguo Fu ◽  
...  

Abstract Barnyardgrass (Echinochloa crus-galli) is a noxious grass weed which infests rice fields and causes huge crop yield losses. In this study, we collected twelve E. crus-galli populations from rice fields of Ningxia province in China and investigated the resistance levels to acetolactate synthase (ALS) inhibitor penoxsulam and acetyl-CoA carboxylase (ACCase) inhibitor cyhalofop-butyl. The results showed that eight populations exhibited resistance to penoxsulam and four populations evolved resistance to cyhalofop-butyl. Moreover, all of the four cyhalofop-butyl-resistant populations (NX3, NX4, NX6 and NX7) displayed multiple-herbicide-resistance (MHR) to both penoxsulam and cyhalofop-butyl. The alternative herbicides bispyribac-sodium, metamifop and fenoxaprop-P-ethyl cannot effectively control the MHR plants. To characterize the molecular mechanisms of resistance, we amplified and sequenced the target-site encoding genes in resistant and susceptible populations. Partial sequences of three ALS genes and six ACCase genes were examined. A Trp-574-Leu mutation was detected in EcALS1 and EcALS3 in two high-level (65.84- and 59.30-fold) penoxsulam-resistant populations NX2 and NX10, respectively. In addition, one copy (EcACC4) of ACCase genes encodes a truncated aberrant protein due to a frameshift mutation in E. crus-galli populations. None of amino acid substitutions that are known to confer herbicide resistance were detected in ALS and ACCase genes of MHR populations. Our study reveals the widespread of multiple-herbicide resistant E. crus-galli populations at Ningxia province of China that exhibit resistance to several ALS and ACCase inhibitors. Non-target-site based mechanisms are likely to be involved in E. crus-galli resistance to the herbicides, at least in four MHR populations.


Sign in / Sign up

Export Citation Format

Share Document