Antibacterial Activity of Fruiting Body Extracts from Culinary-Medicinal Winter Mushroom, Flammulina velutipes (Agaricomycetes) against Oral Pathogen Streptococcus mutans

This study was focused on evaluating the bactericidal and anti-adhesive efficacy of silver-hydroxyapatite/ titania nanocomposites (nAg-HA/TiO2) coating on titanium against oral bacteria. Porphyromonas gingivalis, Prevotella intermedia and Fusohacterium nucleatum and Streptococcus mutans were used. Antibacterial activity of nAg-HA/TiO2 coating was investigated quantitatively using film applicator coating method and titanium plates incubated with bacteria were prepared for SEM to observe the adherence of oral bacteria. The viability of each type of bacteria on the antibacterial film was suppressed to about 10% after anaerobic incubation for 3 hours. Image of SEM demonstrated that bacteria on sandblasting surfaces were relatively confluent whilst on coated surfaces fewer bacteria were observed. Adherence of bacteria on nAg-HA / TiO2-coated surfaces compared with uncoated surfaces was remarkably decreased.

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Analisa Aktivitas Antibakteri Rebusan Daun Sirih Dengan Rebusan Daun Kemangi Terhadap Pertumbuhan Bakteri Streptococcus mutans

Hang Tuah Medical journal ◽

10.30649/htmj.v18i1.459 ◽

2020 ◽

Vol 18 (1) ◽

pp. 36

Author(s):

ENNY WILLIANTI ◽

THEODORA THEODORA ◽

WAHYUNI DYAH PARMASARI

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Streptococcus Mutans ◽

Observational Research ◽

Ocimum Sanctum ◽

Piper Betle ◽

Oral Flora ◽

Betel Leaf ◽

Growth Of Bacteria

ABSTRACT Background: Betel leaf contains essential oils consisting of bethelphenol, kavikol, sesquiterpenes, hydroxycavikol, cavibetol, estragol, eugenol and carvacrol. Essential oils are antibacterial due to the presence of phenol compounds and their derivatives that can denature the bacterial cell proteins. Basil leaves contain compounds from essential oils, namely 1,8-cineole, ß-bisabolene, and methyl eugenol. These three ingredients are soluble to ethanol and can cause damage to the cell membranes of the Streptococcus mutans bacteria, which are members of the normal oral flora but can turn into pathogens if the balance of normal flora is disturbed. The aim of this study was to determine the difference in the activity of the antibacterial of decoction betel leaf (piper betle L. ) with a decoction of basil leaves (ocimum sanctum) against growth of bacteria Streptococcus mutans (in vitro study).Method: this observational research with disk diffusion techniques. This study observed and measured the diameter of the inhibitory zone in MHA formed by decoction of betel leaf (piper betle L) and basil leaf (ocimum sanctum) in units of millimeters (mm). There were 2 groups with 16 replications.Results: the results of the description test showed that the antibacterial activity of the betel leaf decoction and the highest decoction of basil leaf was 17 mm and the lowest was 15 mm, but the average antibacterial value of betel leaf decoction (15,81) greater than the average value of antibacterial activity of basil leaf (15.75). This is because there are chemicals contained in betel leaf similar as contained in basil leaf, namely essential oils.Conclusion: there is no difference in the antibacterial activity of decoction betel leaf with decoction basil leaf against growth of bacteria Streptococcus mutans. Keywords: Betel leaf decoction, basil leaf decoction, Streptococcus mutans. Abstrak Latar Belakang: Daun sirih mengandung minyak atsiri yang terdiri dari bethelphenol, kavikol, seskuiterpen, hydroxycavikol,cavibetol, estragol, eugenol dan carvacrol. Minyak atsiri bersifat antibakteri karena adanya senyawa phenol dan turunannya yang dapat mendenaturasi protein sel bakteri. Daun kemangi mengandung senyawa dari minyak atsiri yaitu 1,8-cineole, ß-bisabolene, metyl eugenol. Ketiga bahan tersebut memiliki sifat larut terhadap etanol dan dapat menyebabkan kerusakan membran sel bakteri streptococcus mutans yang merupakan anggota flora normal rongga mulut tetapi dapat berubah menjadi patogen jika keseimbangan flora normal terganggu.Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antibakteri rebusan daun sirih (piper betle L) dengan rebusan daun kemangi (ocimum sanctum) terhadap pertumbuhan bakteri Streptococcus mutans (penelitian in vitro).Metode: penelitian observasional ini dengan teknik difusi. Penelitian ini dilakukan dengan mengamati dan mengukur diameter zona hambat pada MHA yang dibentuk oleh rebusan daun sirih (piper betle L) dan daun kemangi (ocimum sanctum) dalam satuan milimeter (mm). Terdapat 2 kelompok dengan replikasi sebanyak 16.Hasil : Hasil uji deskripsi menunjukkan bahwa aktivitas antibakteri pada rebusan daun sirih maupun rebusan daun kemangi yang tertinggi sebesar 17 mm dan yang terendah 15 mm. Tetapi pada nilai rata-rata efektifitas antibakteri rebusan daun sirih (15,81) lebih besar daripada nilai rata-rata efektifitas antibakteri rebusan daun kemangi (15,75). Hal ini dikarenakan ada zat kimia yang terkandung dalam daun sirih mirip dengan yang terkandung dalam daun kemangi, yaitu minyak atsiri.Kesimpulan : tidak ada perbedaan aktivitas antibakteri rebusan daun sirih dengan rebusan daun kemangi terhadap pertumbuhan bakteri Streptococcus mutans. Kata kunci: rebusan daun sirih, rebusan daun kemangi, Streptococcus mutans.

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Anti-bacterial activity of three essential oils - An in vitro study

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i2.380 ◽

2019 ◽

Vol 10 (2) ◽

pp. 1049-1053 ◽

Cited By ~ 1

Author(s):

Geetha RV ◽

John Rozar Raj B ◽

Lakshmi Thangavelu

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Streptococcus Mutans ◽

Bacterial Species ◽

In Vitro Study ◽

Oral Bacteria ◽

Zone Of Inhibition ◽

Thyme Oil ◽

Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

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Antibacterial Activity of Ginger (Zingiber officinale) Leaves Essential Oil Nanoemulsion against the Cariogenic Streptococcus mutans

Journal of Applied Pharmaceutical Science ◽

10.7324/japs.2018.8906 ◽

2018 ◽

Vol 8 (9) ◽

pp. 34-41 ◽

Cited By ~ 7

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Streptococcus Mutans ◽

Zingiber Officinale

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Minireview: Formulasi Obat Kumur Ekstrak Daun Ketul (Bidens pilosa)

KELUWIH: Jurnal Sains dan Teknologi ◽

10.24123/saintek.v2i1.3986 ◽

2021 ◽

Vol 2 (1) ◽

Author(s):

Zidane Irwansyah ◽

Hadinata Santoso ◽

Mariana Wahjudi

Keyword(s):

Antibacterial Activity ◽

Streptococcus Mutans ◽

Cardiac Glycosides ◽

Antibacterial Properties ◽

Tween 80 ◽

Peppermint Oil ◽

Literature Study ◽

Bidens Pilosa ◽

Extraction Solvent ◽

Water Viscosity

Abstract— Ketul (Bidens pilosa L) is weed plant that has antibacterial properties. The potential of this plant has not been developed yet in Indonesia. We conducted a literature study about this plant use for herbal mouthwash. The purposes of this study were to determine components of Ketul leaf with antibacterial activity, to know the extraction solvent, to determine the method for antibacterial activity, and to predict the characteristics and components of herbal mouthwash formula. The results showed that Ketul leaf contains tannins, cardiac glycosides, saponins, alkaloids, flavonoids, and terpenoids which could inhibit the growth of various bacteria, including Streptococcus mutans. Hence the mouthwash is predicted to have antibacterial activity. The safe extraction solvent widely used was 70-80% ethanol. The Ketul leaf extract has the potential to be applied in herbal mouthwash formula. Other characteristic can be adjusted to meet the requirements as herbal mouthwash, which are pH between 5-7, the viscosity value near water viscosity value (1cP), stable during storage, and color is brownish yellow. Some common additives were added in mouthwash to improve the flavors and taste, such as sorbitol, tween-80, peppermint oil, and sodium benzoate. Keywords: antibacterial activity, solvent, Streptococcus mutans, weed Abstrak— Ketul (Bidens pilosa L) merupakan tumbuhan gulma yang memiliki aktivitas antibakteri. Potensi tumbuhan ini belum banyak dikembangkan di Indonesia. Pada tulisan ini telah dilakukan kajian pustaka pemanfaatan Ketul untuk obat kumur herbal. Tujuan kajian ini adalah untuk mengetahui komponen ekstrak daun Ketul yang berkhasiat antibakteri, mengetahui pelarut ekstraksi, metode penentuan aktivitas antibakteri obat kumur, dan prediksi karakteristik dan komponen obat kumur herbal daun Ketul. Hasil penelusuran pustaka menunjukkan bahwa daun Ketul mengandung tannin, glikosida jantung, saponin, alkaloid, flavonoid, dan terpenoid, yang dapat menghambat pertumbuhan berbagai bakteri, termasuk Streptococcus mutans sehingga obat kumur kemungkinan memiliki aktivitas antibakteri. Pelarut aman yang banyak digunakan untuk ekstraksi komponen fitokimia tersebut adalah etanol 70-80%. Penentuan daya antibakteri dapat dilakukan menggunakan metode difusi agar atau pengenceran. Ekstrak daun Ketul berpotensi digunakan dalam formula obat kumur herbal. Selain potensi antibakterinya, karakteristik lainnya dapat diatur untuk memenuhi syarat formula obat kumur herbal yaitu pH berkisar 5-7, nilai viskositas mendekati nilai viskositas air (1cP), stabilitas dapat tetap terjaga selama penyimpanan dan kemungkinan obat kumur berwarna kuning kecoklatan. Beberapa bahan tambahan, seperti sorbitol, tween-80, peppermint oil, dan natrium benzoate, merupakan bahan tambahan yang aman dan umum digunakan untuk menambah cita rasa dan stabilitas formula obat kumur herbal ekstrak daun Ketul. Kata kunci: aktivitas antibakteri, gulma, pelarut, Streptococcus mutans

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Comparative Study of Ethanolic Wild African Nutmeg (Pycnanthus angolensis (Welw.) Stem Bark Extract Potentials and Selected Conventional Toothpaste against Hidden Resident Mouth Cavity Microfora

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2020/v6i230147 ◽

2020 ◽

pp. 10-36

Author(s):

Mouth cavity Microfora. ◽

Teniola Temitayo Mary

Keyword(s):

Antibacterial Activity ◽

Streptococcus Mutans ◽

Frequency Distribution ◽

Stem Bark ◽

Diffusion Method ◽

Bark Extract ◽

Percentage Frequency ◽

Mouth Cavity ◽

Stem Bark Extract ◽

Streptococcus Ratti

The aim of the study is to evaluate and compare the antibacterial activity of ethanolic stem extract of (Wild African nutmeg) Pycnanthus angolensis (Welw.) and some commercially available toothpaste against bacteria isolated from the hidden resident mouth cavity microfora. Bacteria were isolated from swabs of apparently healthy individuals and were identified using Staining procedure biochemical tests and the use of Bergey’s manual of bacteria identification The assay for antibacterial activity of Pycnanthus angolensis stem bark extract and the four toothpastes were determined using agar well diffusion method. The Gram positive bacteria isolated were Streptococcus sangus, Streptococcus ratti, Stomatococcus mucilaginous., Peptostreptococcus sp., and Streptococcus mutans and the Gram negative bacteria were Veillonella atypical, Veillonella parvula, Veillonella dispar and Acidiaminococcus sp. Oral B toothpaste showed maximum efficacy of inhibition with inhibition zone diameter as wide as 20 mm at 100 mg/ml. Percentage frequency distribution of antibacterial activity of conventional toothpaste (Close-up) against hidden resident mouth cavity microfora depicts Acidaminococcus sp.13%, Veillonella parvula (10%), Veillonella dispar (12%), Peptostreptococcus sp.(12%), Stomatococcus mucilaginous.(9%), Streptococcus ratti (13%), Veillonella atypical (11%), Streptococcus sangus (9%) and Streptococcus mutans (11%), Percentage frequency distribution of antibacterial activity of conventional toothpaste (Oral B toothpaste) against hidden resident mouth cavity microfora reveals Acidaminococcus sp.(11%,) Veillonella dispar (11%), Veillonella parvula (10%), Peptostreptococcus sp. (12%), Stomatococcus mucilaginous.(15%), Streptococcus ratti (11%), Veillonella atypical (8%), Streptococcus sangus (10%), and Streptococcus mutans (12%), Percentage frequency distribution of antibacterial activity of conventional toothpaste (MyMy toothpaste) against hidden resident mouth cavity microfora depicts Acidaminococcus sp.(12%), Veillonella dispar (9%), Veillonella parvula (8%), Peptostreptococcus sp.(10%), Stomatococcus mucilaginous.(16%), Streptococcus ratti (9%), Veillonella atypical (15%),Streptococcus sangus (9%) and Streptococcus mutans (12%), Percentage frequency distribution of antibacterial activity of conventional toothpaste (Olive toothpaste) against hidden resident mouth cavity microfora shows Acidaminococcus sp.(9%), Veillonella dispar (10%), Veillonella parvula (10%), Peptostreptococcus sp.(12%), Stomatococcus mucilaginous.(13%), Streptococcus ratti (10%) ,Veillonella atypical (17%), Streptococcus sangus (7%), and Streptococcus mutans (12%). Pycnanthus Angolensis stem bark extract inhibited the growth of the oral bacterial isolates with of zones of inhibition diameter ranging from 6 mm to 17 mm at a concentration of 100mg/ml. Secondary metabolite (Phytochemical) screening shows the presence of flavonoids, tannins, saponins, alkaloids, reducing sugars, steroid, phenol, terpenoid, pyrrolozidine alkaloid, glycoside and cardiac glycoside with glycoside and terpenoid most present. However, anthraquinones and volatile oil were absent. With menial antibacterial activity, P. angolensis can be use in the formulation of herbal toothpaste. It should be advocated that Pycnanthus angolensis should be added to our convention toothpaste to improve the functional ingredient of the toothpaste and Plant-based traditional knowledge has become a recognized tool in search for new sources of drugs. It is clear that the use of these herbal plants can offer a platform for further research.

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