The Frequency of Allelic Polymorphism of Matrix Gla-Protein Gene in Patients with Acute Coronary Syndrome

Vascular calcification contributes to the pathogenesis of coronary artery disease while matrix Gla protein (MGP) was recently identified as a potent inhibitor of vascular calcification. MGP fractions, such as dephosphorylated-uncarboxylated MGP (dp-ucMGP), lack post-translational modifications and are less efficient in vascular calcification inhibition. We sought to compare dp-ucMGP levels between patients with acute coronary syndrome (ACS), stratified by ST-elevation myocardial infarction (STEMI) and non-ST-elevation myocardial infarction (NSTEMI) status. Physical examination and clinical data, along with plasma dp-ucMGP levels, were obtained from 90 consecutive ACS patients. We observed that levels of dp-ucMGP were significantly higher in patients with NSTEMI compared to STEMI patients (1063.4 ± 518.6 vs. 742.7 ± 166.6 pmol/L, p < 0.001). NSTEMI status and positive family history of cardiovascular diseases were only independent predictors of the highest tertile of dp-ucMGP levels. Among those with NSTEMI, patients at a high risk of in-hospital mortality (adjudicated by GRACE score) had significantly higher levels of dp-ucMGP compared to non-high-risk patients (1417.8 ± 956.8 vs. 984.6 ± 335.0 pmol/L, p = 0.030). Altogether, our findings suggest that higher dp-ucMGP levels likely reflect higher calcification burden in ACS patients and might aid in the identification of NSTEMI patients at increased risk of in-hospital mortality. Furthermore, observed dp-ucMGP levels might reflect differences in atherosclerotic plaque pathobiology between patients with STEMI and NSTEMI.

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Distribution of allelic variants of genes inhibitors and activators ectopic calcification in patients with acute coronary syndrome

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v21.857 ◽

1970 ◽

Vol 21 ◽

pp. 306-310

Author(s):

V. Yu. Harbuzova ◽

O. A. Obukhova ◽

I. O. Rozumenko ◽

Ye. I. Dubovyk ◽

T. M. Oleshko ◽

...

Keyword(s):

Acute Coronary Syndrome ◽

Venous Blood ◽

Control Group ◽

Ectopic Calcification ◽

Allelic Polymorphism ◽

Coronary Syndrome ◽

Major Allele ◽

Polymorphic Variants ◽

Sclerotic Lesions ◽

Hereditary Susceptibility

Aim. The study of the distribution of polymorphic variants K121Q (gene ENPP1), T134967G (gene ANKH) and A69314G (gene TNAP) is in patients with acute coronary syndrome. Methods. Venous blood of 118 patients with ACS and 110 persons of control group were genotyped for the polymorphism by PCR and restriction fragment length polymorphism method. Results. There is an association between acute coronary syndrome and polymorphic variants of genes TNAP (A69314G) and ANKH (T134967G). ACS risk in carriers of the minor allele of A69314G polymorphism to 2.2 times (P = 0.013; OR = 2.244), and for T134967G polymorphism to 1.9 times (P = 0.024; OR = 1.857) higher than homozygotes for the major allele. Conclusions. Allelic polymorphism gene activators and inhibitors of ectopic calcification is an important factor in hereditary susceptibility to sclerotic lesions of arteries and their complications. Keywords: gene polymorphism, calcification, acute coronary syndrome.

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CORRELATION OF ARACHIDONATE 5-LIPOXYGENASE ACTIVATING PROTEIN GENE SG13S114T/A POLYMORPHISM WITH ACUTE CORONARY SYNDROME IN MALE

Heart ◽

10.1136/heartjnl-2012-302920k.24 ◽

2012 ◽

Vol 98 (Suppl 2) ◽

pp. E189.3-E190

Author(s):

He Guo-ping ◽

Hui Jing-jiao ◽

Shen Dan-dan ◽

He Guo-ping

Keyword(s):

Acute Coronary Syndrome ◽

Protein Gene ◽

Coronary Syndrome

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OP-161 [AJC » Acute Coronary Syndromes] Enhanced Serum Levels of Matrix Gla Protein and Bone Morphogenetic Protein in Acute Coronary Syndrome Patients

The American Journal of Cardiology ◽

10.1016/j.amjcard.2017.03.077 ◽

2017 ◽

Vol 119 (8) ◽

pp. e15

Author(s):

Zafer Buyukterzi ◽

Ummugulsum Can ◽

Mehmet Sertac Alpaydin ◽

Asuman Guzelant ◽

Sukru Karaarslan ◽

...

Keyword(s):

Acute Coronary Syndrome ◽

Bone Morphogenetic Protein ◽

Acute Coronary Syndromes ◽

Serum Levels ◽

Matrix Gla Protein ◽

Coronary Syndrome ◽

Morphogenetic Protein ◽

Coronary Syndromes

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Thr83ala Gene Polymorphism Association with Arterial Calcification, Acute Coronary Syndrome and Ischemic Strokes in Older Adults

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2018.297 ◽

2018 ◽

Vol 6 (8) ◽

pp. 1365-1369

Author(s):

Yurii O. Ataman ◽

Albina V. Zharkova ◽

Andrii V. Grek ◽

Tetiana M. Rudenko ◽

Maryna A. Gordina

Keyword(s):

Gene Polymorphism ◽

Protein Gene ◽

Statistical Significance ◽

Lower Extremities ◽

The Elderly ◽

Matrix Gla Protein ◽

Arterial Calcification ◽

Homozygous State ◽

Coronary Syndrome ◽

Comparison Groups

BACKGROUND: Calcification of the arteries of the lower extremities is a very common pathological process, which has an independent significance in the development of cardiovascular diseases. There is evidence that development of calcification of the arteries correlates with a mutation of the MGP protein gene representing by the Thr83Ala polymorphism.AIM: The purpose of the study was to analyse the connection of Thr83Ala polymorphism of the MGP gene with the development of calcification of the arteries of the lower extremities.METHODS: The study involved 80 patients. Half of them had signs of calcification of the arteries of the lower extremities. The allelic Thr83Ala polymorphism of the MGP protein gene was determined by polymerase chain reaction, establishing the presence of calcification of the arteries by radiological and dopplerographic methods.RESULTS: The study aimed to analyse the association of the Thr83Ala polymorphism of the matrix Gla protein gene with the development of calcification of the arteries of the lower extremities. The data obtained suggest that the replacement of threonine by alanine in the 83rd position of the MGP molecule can affect the functional properties of the protein and in particular its anticarcinogenic properties. Although there was no difference in the distribution of different variants of the genotype by Thr83Ala to the MGP gene polymorphism in patients with CA and healthy patients, but in the distribution of genotypes in the comparison groups separated by sex, it was found that in women, carriage of the Ala allele in a homozygous state is a factor, which protects the development of arterial calcination in the elderly and senile.CONCLUSION: Differences in the distribution of different variants of the genotype according to Thr83Ala to the polymorphism of the MGP gene between patients with CAD and healthy patients do not exceed the limits of statistical significance. In the distribution of genotypes in the comparison groups divided by sex, it was found that in women the carrier of the Ala-allele in the homozygous state is a factor that prevents the development of Menkeberg arteriosclerosis in the elderly and old age.

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Correlation of CYP2D6 allelic polymorphism to outcome of acute coronary syndrome in mid-Euphrates Iraqi patients on metoprolol therapy

Gene ◽

10.1016/j.gene.2019.04.012 ◽

2019 ◽

Vol 703 ◽

pp. 112-119

Author(s):

Asma A. Swadi ◽

Bassim I. Mohammad ◽

Najah R. Hadi ◽

Hayder A. Al-Aubaidy

Keyword(s):

Acute Coronary Syndrome ◽

Allelic Polymorphism ◽

Coronary Syndrome

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Relationship between 5-lipoxygenase activating protein gene SG13S89G/A polymorphism and acute coronary syndrome

Heart ◽

10.1136/heartjnl-2011-300867.129 ◽

2011 ◽

Vol 97 (Suppl 3) ◽

pp. A45-A46

Author(s):

H. Guoping ◽

H. Jingjiao ◽

S. Dandan

Keyword(s):

Acute Coronary Syndrome ◽

Protein Gene ◽

Coronary Syndrome

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Vitamin D binding protein polymorphysm in patients with acute coronary syndrome in kaliningrad region

Obesity and metabolism ◽

10.14341/omet9758 ◽

2019 ◽

Vol 16 (3) ◽

pp. 81-87

Author(s):

Robert S. Bogachev ◽

Anastasia Y. Kozel ◽

Larisa S. Litvinova ◽

Larisa V. Mikhailova ◽

Elena S. Shytova ◽

...

Keyword(s):

Diabetes Mellitus ◽

Vitamin D ◽

Acute Coronary Syndrome ◽

Coronary Artery ◽

Protein Gene ◽

Malignant Tumors ◽

Binding Protein ◽

Vitamin D Binding Protein ◽

Coronary Syndrome ◽

Binding Protein Gene

BACKGROUND: Vitamin D binding protein is a main vitamin D carrier in serum. It also has an impact on macrophagial function. Role of vitamin D and macrophages in the pathogenesis of atherosclerosis is scientifically proven but there is lack of data on vitamin D binding protein in this regard. AIMS: To evaluate the vitamin D binding protein polymorphism in patients with acute coronary syndrome without diabetes mellitus, autoimmune diseases and malignant tumors. Determine correlation, if there is, between vitamin D binding protein allele and features of acute coronary syndrome among this patient group. MATERIALS AND METHODS: It is a cross-sectional observational study. Study subjects are patients with acute coronary syndrome. Exclusion criteria are the presence of diabetes mellitus, autoimmune diseases and malignant tumors. In all participants were evaluated: predisposing factors for heart diseases, CBC, biochemical blood test, troponin, coronarography, echocardiography. The study lasted for 5 months from November 2017 until March 2018. Primary end point assessment of vitamin D binding protein polymorphysm in this group of patients with acute coronary syndrome by means of vitamin D binding protein gene sequencing. 50 patients were enrolled into this study who were urgently admitted to hospital and diagnosed with acute coronary syndrome. Among them 36 males and 14 females. Mean age was 60 (55;66) years. All participants were sequenced for single nucleotide polymorphysm in VDBP p.T436K (rs4588) and P.432E (rs7041). RESULTS: Gene polymorphysms of interest were found in 43 patients among 50 enrolled. Haplotype Gc1s/2 (rs7041G-rs4588A) was found in 7 (14%) patients, Gc2 (rs7041T-rs4588A) in 9 (18%) patients, Gc1s (rs7041G-rs4588C) in 20 (40%) patients, Gc1f (rs7041T-rs4588C) in 14 (28%). Coronarography showed that coronary artery occlusions obstructing more than 50% of vessel lumen was found in 16 patients; obstruction greater than 90% was seen in 8 patients; total occlusion in 4 patients. CONCLUSIONS: In patient group with acute coronary syndrome prevalence of vitamin D binding protein gene polymorphysm was high in 86% of participants. The features of Gc2 haplotype were higher frequency of recurrent myocardial infarction and total coronary artery occlusion, as well as tendency to decreased serum vitamin D3 (25(OH)D) levels.

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