Ex Vivo Study Comparing Three Cold Atmospheric Plasma (CAP) Sources for Biofilm Removal on Microstructured Titanium

Saskia Preissner; Ann Cathrin Poehlmann; Andreas Schubert; Antje Lehmann; Thomas Arnold; Olaf Nell; Stefan Rupf

doi:10.1615/plasmamed.2018027314

Cold atmospheric plasma treatment inhibits growth in colorectal cancer cells

Biological Chemistry ◽

10.1515/hsz-2018-0193 ◽

2018 ◽

Vol 400 (1) ◽

pp. 111-122 ◽

Cited By ~ 10

Author(s):

Christin Schneider ◽

Stephanie Arndt ◽

Julia L. Zimmermann ◽

Yangfang Li ◽

Sigrid Karrer ◽

...

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Plasma Treatment ◽

Cancer Cells ◽

Ex Vivo ◽

Atmospheric Plasma ◽

Normal Colon ◽

Colon Tissue ◽

Cold Atmospheric Plasma ◽

Colorectal Cancer Cells

AbstractPlasma oncology is a relatively new field of research. Recent developments have indicated that cold atmospheric plasma (CAP) technology is an interesting new therapeutic approach to cancer treatment. In this study, p53 wildtype (LoVo) and human p53 mutated (HT29 and SW480) colorectal cancer cells were treated with the miniFlatPlaSter – a device particularly developed for the treatment of tumor cells – that uses the Surface Micro Discharge (SMD) technology for plasma production in air. The present study analyzed the effects of plasma on colorectal cancer cellsin vitroand on normal colon tissueex vivo. Plasma treatment had strong effects on colon cancer cells, such as inhibition of cell proliferation, induction of cell death and modulation of p21 expression. In contrast, CAP treatment of murine colon tissueex vivofor up to 2 min did not show any toxic effect on normal colon cells compared to H2O2positive control. In summary, these results suggest that the miniFlatPlaSter plasma device is able to kill colorectal cancer cells independent of their p53 mutation status. Thus, this device presents a promising new approach in colon cancer therapy.

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Ex vivo human skin experiments for the evaluation of safety of new cold atmospheric plasma devices

Clinical Plasma Medicine ◽

10.1016/j.cpme.2012.10.001 ◽

2013 ◽

Vol 1 (1) ◽

pp. 36-44 ◽

Cited By ~ 40

Author(s):

G. Isbary ◽

J. Köritzer ◽

A. Mitra ◽

Y.-F. Li ◽

T. Shimizu ◽

...

Keyword(s):

Human Skin ◽

Ex Vivo ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

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Efficiency of cold atmospheric plasma, cleaning powders and their combination for biofilm removal on two different titanium implant surfaces

Clinical Oral Investigations ◽

10.1007/s00784-021-04300-0 ◽

2022 ◽

Author(s):

Julia Kamionka ◽

Rutger Matthes ◽

Birte Holtfreter ◽

Christiane Pink ◽

Rabea Schlüter ◽

...

Keyword(s):

Ex Vivo ◽

Atmospheric Pressure Plasma ◽

Titanium Implant ◽

Bacterial Biofilm ◽

Atmospheric Plasma ◽

Cleaning Efficiency ◽

Implant Surface ◽

Air Polishing ◽

Biofilm Removal ◽

Cold Atmospheric Pressure Plasma

Abstract Objectives Biofilm removal is the decisive factor for the control of peri-implantitis. Cold atmospheric pressure plasma (CAP) can become an effective aid due to its ability to destroy and to inactivate bacterial biofilm residues. This study evaluated the cleaning efficiency of CAP, and air-polishing with glycine (APG) or erythritol (APE) containing powders alone or in combination with CAP (APG + CAP, APE + CAP) on sandblasted/acid etched, and anodised titanium implant surface. Materials and methods On respective titanium discs, a 7-day ex vivo human biofilm was grown. Afterwards, the samples were treated with CAP, APG, APE, APG + CAP, and APE + CAP. Sterile and untreated biofilm discs were used for verification. Directly after treatment and after 5 days of incubation in medium at 37 °C, samples were prepared for examination by fluorescence microscopy. The relative biofilm fluorescence was measured for quantitative analyses. Results Air-polishing with or without CAP removed biofilms effectively. The combination of air-polishing with CAP showed the best cleaning results compared to single treatments, even on day 5. Immediately after treatment, APE + CAP showed insignificant higher cleansing efficiency than APG + CAP. Conclusions CAP supports mechanical cleansing and disinfection to remove and inactivate microbial biofilm on implant surfaces significantly. Here, the type of the powder was not important. The highest cleansing results were obtained on sandblasted/etched surfaces. Clinical relevance. Microbial residuals impede wound healing and re-osseointegration after peri-implantitis treatment. Air-polishing treatment removes biofilms very effectively, but not completely. In combination with CAP, microbial free surfaces can be achieved. The tested treatment regime offers an advantage during treatment of peri-implantitis.

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COLD ATMOSPHERIC PLASMA DIFFERENTIALLY AFFECTS CELL RENEWAL AND DIFFERENTIATION OF STEM CELLS AND APC-DEFICIENT-DERIVED TUMOR CELLS IN INTESTINAL ORGANOIDS

10.1101/2021.10.13.464287 ◽

2021 ◽

Author(s):

Alia Hadefi ◽

Morgane Leprovots ◽

Max Thulliez ◽

Orianne Bastin ◽

Anne Lefort ◽

...

Keyword(s):

Stem Cells ◽

Tumor Cells ◽

Ex Vivo ◽

Biological Effects ◽

Three Dimensional ◽

Atmospheric Plasma ◽

Cell Renewal ◽

Epithelial Stem Cells ◽

Cold Atmospheric Plasma ◽

Ex Vivo Culture

Cold atmospheric plasma (CAP) treatment has been proposed as a potentially innovative therapeutic tool in the biomedical field, notably for cancer due to its proposed toxic selectivity on cancer cells versus healthy cells. In the present study, we addressed the relevance of three-dimensional organoid technology to investigate the biological effects of CAP on normal epithelial stem cells and tumor cells isolated from mouse small intestine. CAP treatment exerted dose-dependent cytotoxicity on normal organoids and induced major transcriptomic changes associated with global response to oxidative stress, fetal-like regeneration reprogramming and apoptosis-mediated cell death. Moreover, we explored the potential selectivity of CAP on tumor-like Apc-deficient versus normal organoids in the same genetic background. Unexpectedly, tumor organoids exhibited higher resistance to CAP treatment, correlating with higher antioxidant activity at baseline as compared to normal organoids. This pilot study suggests that the ex vivo culture system could be a relevant alternative model to further investigate translational medical applications of CAP technology.

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Ex vivo characterization of thermal argon plasma sources for potential cold atmospheric plasma application in medicine

European Journal of Obstetrics & Gynecology and Reproductive Biology ◽

10.1016/j.ejogrb.2018.08.339 ◽

2019 ◽

Vol 234 ◽

pp. e83-e84

Author(s):

Andrina Kölle ◽

Michael Ackermann ◽

Raphael Utz ◽

Michael Haupt ◽

Jakob Barz ◽

...

Keyword(s):

Ex Vivo ◽

Argon Plasma ◽

Atmospheric Plasma ◽

Plasma Sources ◽

Cold Atmospheric Plasma

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Comparison of the effects of air-powder abrasion, chemical decontamination, or their combination in open-flap surface decontamination of implants failed for peri-implantitis: an ex vivo study

Clinical Oral Investigations ◽

10.1007/s00784-020-03578-w ◽

2020 ◽

Author(s):

Nicola Pranno ◽

Maria Paola Cristalli ◽

Fabio Mengoni ◽

Ilaria Sauzullo ◽

Susanna Annibali ◽

...

Keyword(s):

Ex Vivo ◽

Bacterial Biofilm ◽

Control Group ◽

Microbiological Analysis ◽

Chemical Decontamination ◽

Surgical Exposure ◽

Biofilm Removal ◽

Significant Difference ◽

Surface Decontamination ◽

Ex Vivo Study

Abstract Objectives To compare, using an ex vivo model, the biofilm removal of three surface decontamination methods following surgical exposure of implants failed for severe peri-implantitis. Materials and methods The study design was a single-blind, randomized, controlled, ex vivo investigation with intra-subject control. Study participants were 20 consecutive patients with at least 4 hopeless implants, in function for >12 months and with progressive bone loss exceeding 50%, which had to be explanted. Implants of each patient were randomly assigned to the untreated control group or one of the three decontamination procedures: mechanical debridement with air-powder abrasion, chemical decontamination with hydrogen peroxide and chlorhexidine gluconate, or combined mechanical-chemical decontamination. Following surgical exposure, implants selected as control were retrieved, and afterwards, test implants were decontaminated according to allocation and carefully explanted with a removal kit. Microbiological analysis was expressed in colony-forming-units (CFU/ml). Results A statistically significant difference (p < 0.001) in the concentrations of CFU/ml was found between implants treated with mechanical debridement (531.58 ± 372.07) or combined mechanical-chemical decontamination (954.05 ± 2219.31) and implants untreated (37,800.00 ± 46,837.05) or treated with chemical decontamination alone (29,650.00 ± 42,596.20). No statistically significant difference (p = 1.000) was found between mechanical debridement used alone or supplemented with chemical decontamination. Microbiological analyses identified 21 microbial species, without significant differences between control and treatment groups. Conclusions Bacterial biofilm removal from infected implant surfaces was significantly superior for mechanical debridement than chemical decontamination. Clinical relevance The present is the only ex vivo study based on decontamination methods for removing actual and mature biofilm from infected implant surfaces in patients with peri-implantitis.

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