scholarly journals Efficiency of cold atmospheric plasma, cleaning powders and their combination for biofilm removal on two different titanium implant surfaces

2022 ◽  
Author(s):  
Julia Kamionka ◽  
Rutger Matthes ◽  
Birte Holtfreter ◽  
Christiane Pink ◽  
Rabea Schlüter ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Atmospheric Pressure Plasma ◽  
Titanium Implant ◽  
Bacterial Biofilm ◽  
Atmospheric Plasma ◽  
Cleaning Efficiency ◽  
Implant Surface ◽  
Air Polishing ◽  
Biofilm Removal ◽  
Cold Atmospheric Pressure Plasma

Abstract Objectives Biofilm removal is the decisive factor for the control of peri-implantitis. Cold atmospheric pressure plasma (CAP) can become an effective aid due to its ability to destroy and to inactivate bacterial biofilm residues. This study evaluated the cleaning efficiency of CAP, and air-polishing with glycine (APG) or erythritol (APE) containing powders alone or in combination with CAP (APG + CAP, APE + CAP) on sandblasted/acid etched, and anodised titanium implant surface. Materials and methods On respective titanium discs, a 7-day ex vivo human biofilm was grown. Afterwards, the samples were treated with CAP, APG, APE, APG + CAP, and APE + CAP. Sterile and untreated biofilm discs were used for verification. Directly after treatment and after 5 days of incubation in medium at 37 °C, samples were prepared for examination by fluorescence microscopy. The relative biofilm fluorescence was measured for quantitative analyses. Results Air-polishing with or without CAP removed biofilms effectively. The combination of air-polishing with CAP showed the best cleaning results compared to single treatments, even on day 5. Immediately after treatment, APE + CAP showed insignificant higher cleansing efficiency than APG + CAP. Conclusions CAP supports mechanical cleansing and disinfection to remove and inactivate microbial biofilm on implant surfaces significantly. Here, the type of the powder was not important. The highest cleansing results were obtained on sandblasted/etched surfaces. Clinical relevance. Microbial residuals impede wound healing and re-osseointegration after peri-implantitis treatment. Air-polishing treatment removes biofilms very effectively, but not completely. In combination with CAP, microbial free surfaces can be achieved. The tested treatment regime offers an advantage during treatment of peri-implantitis.

scholarly journals Characterisation of a Cold Atmospheric Pressure Plasma Torch for Medical Applications: Demonstration of Device Safety

2021 ◽  
Vol 11 (24) ◽  
pp. 11864
Author(s):  
Adam Bennett ◽  
Takuya Urayama ◽  
Konstantinos Papangelis ◽  
Peter Yuen ◽  
Nan Yu
Keyword(s):  
Plasma Torch ◽  
Treatment Time ◽  
Atmospheric Pressure Plasma ◽  
Atmospheric Plasma ◽  
Medical Applications ◽  
Regulatory Body ◽  
Exposure Limits ◽  
Treatment Area ◽  
Safety Standards ◽  
Cold Atmospheric Pressure Plasma

The safety and effectiveness of plasma devices are of crucial importance for medical applications. This study presents the novel design of an atmospheric plasma torch (SteriPlas) and its characterisation. The SteriPlas was characterised to ascertain whether it is safe for application on human skin. The emission spectrum discharged from the SteriPlas was shown to be the same as the emission from the MicroPlaSter Beta. The UV emitted from the SteriPlas was measured, and the effective irradiance was calculated. The effective irradiance enabled the determination of the maximum UV exposure limits, which were shown to be over two hours: significantly longer than the current two-minute treatment time. The use of an extraction system with a higher flow rate appears to reduce slightly the effective irradiance at the treatment area. The NOx and ozone emissions were recorded for both SteriPlas configurations. The NOx levels were shown to be orders of magnitude lower than their safety limits. The ozone emissions were shown to be safe 25 mm from the SteriPlas cage. A discussion of how safety standards differ from one regulatory body to another is given.

scholarly journals Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Plasma ◽  
2018 ◽  
Vol 1 (2) ◽  
pp. 261-276 ◽  
Author(s):  
Letizia Crestale ◽  
Romolo Laurita ◽  
Anna Liguori ◽  
Augusto Stancampiano ◽  
Maria Talmon ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Macrophage Polarization ◽  
Immune Surveillance ◽  
Atmospheric Pressure Plasma ◽  
Atmospheric Plasma ◽  
Viability Analysis ◽  
Inflammatory Phenotype ◽  
Cold Atmospheric Pressure Plasma ◽  
Anti Inflammatory ◽  
Inflammatory Macrophages

Monocytes are involved in innate immune surveillance, establishment and resolution on inflammation, and can polarize versus M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. The possibility to control and drive immune cells activity through plasma stimulation is therefore attractive. We focused on the effects induced by cold-atmospheric plasma on human primary monocytes and monocyte-derived macrophages. Monocytes resulted more susceptible than monocyte-derived macrophages to the plasma treatment as demonstrated by the increase in reactive oxygen (ROS) production and reduction of viability. Macrophages instead were not induced to produce ROS and presented a stable viability. Analysis of macrophage markers demonstrated a time-dependent decrease of the M1 population and a correspondent increase of M2 monocyte-derived macrophages (MDM). These findings suggest that plasma treatment may drive macrophage polarization towards an anti-inflammatory phenotype.

Wound-Based Bacterial Biofilm Imaging for Selective Cold Atmospheric Pressure Plasma Treatment

2021 ◽  
Author(s):  
Michael Okebiorun ◽  
Cameron Waite ◽  
Hannah May ◽  
Dalton Miller ◽  
Ken Cornell ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Bacterial Biofilm ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

Parameters That Improve Cleaning Efficiency of Subgingival Air Polishing on Titanium Implant Surfaces: An In Vitro Study

2017 ◽  
Vol 88 (4) ◽  
pp. 407-414 ◽  
Author(s):  
Ceylin S. Tastepe ◽  
Xingnan Lin ◽  
Marcel Donnet ◽  
Daniel Wismeijer ◽  
Yuelian Liu
Keyword(s):  
In Vitro Study ◽  
Titanium Implant ◽  
Vitro Study ◽  
Cleaning Efficiency ◽  
Air Polishing

scholarly journals Ex Vivo Study Comparing Three Cold Atmospheric Plasma (CAP) Sources for Biofilm Removal on Microstructured Titanium

2019 ◽  
Vol 9 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Saskia Preissner ◽  
Ann Cathrin Poehlmann ◽  
Andreas Schubert ◽  
Antje Lehmann ◽  
Thomas Arnold ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Biofilm Removal ◽  
Ex Vivo Study

scholarly journals Possibility to extend the shelf life of NFC tomato juice using cold atmospheric pressure plasma

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Agnieszka Starek ◽  
Agnieszka Sagan ◽  
Dariusz Andrejko ◽  
Barbara Chudzik ◽  
Zbigniew Kobus ◽  
...  
Keyword(s):  
Shelf Life ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Tomato Juice ◽  
Atmospheric Plasma ◽  
Thermal Method ◽  
Digital Microscopy ◽  
Chemical Waste ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

AbstractCold Atmospheric pressure Plasma (CAP) is a non-thermal method used in food processing. CAP generated with the use of nitrogen in a Glide-arc device for 300 to 600 s exhibited high potential for microbial decontamination and did not induce substantial changes in the physicochemical properties of NFC tomato juice. Samples exposed to cold atmospheric plasma had mostly an intact structure, as revealed by digital microscopy. The investigations indicate that CAP can be applied for biological and chemical waste-free decontamination of food and extension of its shelf life.

scholarly journals Osseointegration of Plasma Jet Treated Titanium Implant Surface in an Animal Model

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 1942
Author(s):  
Min-Ho Jang ◽  
Young-Bum Park ◽  
Jae-Sung Kwon ◽  
Yeun-Ju Kim ◽  
Jae-Hoon Lee
Keyword(s):  
Atmospheric Pressure Plasma ◽  
Region Of Interest ◽  
Plasma Jet ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Control Group ◽  
Implant Surface ◽  
Initial Stability ◽  
Maxilla And Mandible ◽  
Experimental Group

Osseointegration of titanium implant is important for the success of both dental and medical implants. Previous studies have attempted to improve osseointegration by considering the use of plasma jet technology, where information with animal models and parameters related to osseointegration is still lacking. Therefore, this study investigated the effects of non-thermal atmospheric pressure plasma jet (NTAPPJ) treatment on titanium implants in terms of osseointegration in mongrel dogs. A total of 41 implants; 21 NTAPPJ treated and 20 control, were placed in the maxilla and mandible of six mongrel dogs for either 4 or 8 weeks. The bone volume (BV) and bone-to-implant contact (BIC) ratio were determined by region of interest (ROI). Statistical analysis was performed with the Wilcoxon rank-sum test. The NTAPPJ group at 4 weeks showed higher numbers in both BV and BIC (p < 0.05) compared to the control group. However, at 8 weeks there were less significant differences between the control or experimental group as the control group had caught up with the experimental group. Hence, NTAPPJ may be an effective treatment for the initial healing period which is critical to ensure reliable long-term predictability. The BV and BIC have been clinically proven to accelerate in the initial stages with the use of NTAPPJ to aid in the healing and initial stability of implants.

scholarly journals Comparison of the effects of air-powder abrasion, chemical decontamination, or their combination in open-flap surface decontamination of implants failed for peri-implantitis: an ex vivo study

2020 ◽  
Author(s):  
Nicola Pranno ◽  
Maria Paola Cristalli ◽  
Fabio Mengoni ◽  
Ilaria Sauzullo ◽  
Susanna Annibali ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Microbiological Analysis ◽  
Chemical Decontamination ◽  
Surgical Exposure ◽  
Biofilm Removal ◽  
Significant Difference ◽  
Surface Decontamination ◽  
Ex Vivo Study

Abstract Objectives To compare, using an ex vivo model, the biofilm removal of three surface decontamination methods following surgical exposure of implants failed for severe peri-implantitis. Materials and methods The study design was a single-blind, randomized, controlled, ex vivo investigation with intra-subject control. Study participants were 20 consecutive patients with at least 4 hopeless implants, in function for >12 months and with progressive bone loss exceeding 50%, which had to be explanted. Implants of each patient were randomly assigned to the untreated control group or one of the three decontamination procedures: mechanical debridement with air-powder abrasion, chemical decontamination with hydrogen peroxide and chlorhexidine gluconate, or combined mechanical-chemical decontamination. Following surgical exposure, implants selected as control were retrieved, and afterwards, test implants were decontaminated according to allocation and carefully explanted with a removal kit. Microbiological analysis was expressed in colony-forming-units (CFU/ml). Results A statistically significant difference (p < 0.001) in the concentrations of CFU/ml was found between implants treated with mechanical debridement (531.58 ± 372.07) or combined mechanical-chemical decontamination (954.05 ± 2219.31) and implants untreated (37,800.00 ± 46,837.05) or treated with chemical decontamination alone (29,650.00 ± 42,596.20). No statistically significant difference (p = 1.000) was found between mechanical debridement used alone or supplemented with chemical decontamination. Microbiological analyses identified 21 microbial species, without significant differences between control and treatment groups. Conclusions Bacterial biofilm removal from infected implant surfaces was significantly superior for mechanical debridement than chemical decontamination. Clinical relevance The present is the only ex vivo study based on decontamination methods for removing actual and mature biofilm from infected implant surfaces in patients with peri-implantitis.

scholarly journals In Vivo Antibacterial Efficacy of Nanopatterns on Titanium Implant Surface: A Systematic Review of the Literature

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1524
Author(s):  
Yang Sun ◽  
Yang Yang ◽  
Weibo Jiang ◽  
Haotian Bai ◽  
He Liu ◽  
...  
Keyword(s):  
Systematic Review ◽  
Antibacterial Activity ◽  
Ex Vivo ◽  
Titanium Implant ◽  
Antibacterial Efficacy ◽  
Inclusion Criteria ◽  
Antibacterial Effects ◽  
Implant Surface

Background: Bionic surface nanopatterns of titanium (Ti) materials have excellent antibacterial effects in vitro for infection prevention. To date, there is a lack of knowledge about the in vivo bactericidal outcomes of the nanostructures on the Ti implant surfaces. Methods: A systematic review was performed using the PubMed, Embase, and Cochrane databases to better understand surface nanoscale patterns’ in vivo antibacterial efficacy. The inclusion criteria were preclinical studies (in vivo) reporting the antibacterial activity of nanopatterns on Ti implant surface. Ex vivo studies, studies not evaluating the antibacterial activity of nanopatterns or surfaces not modified with nanopatterns were excluded. Results: A total of five peer-reviewed articles met the inclusion criteria. The included studies suggest that the in vivo antibacterial efficacy of the nanopatterns on Ti implants’ surfaces seems poor. Conclusions: Given the small number of literature results, the variability in experimental designs, and the lack of reporting across studies, concluding the in vivo antibacterial effectiveness of nanopatterns on Ti substrates’ surfaces remains a big challenge. Surface coatings using metallic or antibiotic elements are still practical approaches for this purpose. High-quality preclinical data are still needed to investigate the in vivo antibacterial effects of the nanopatterns on the implant surface.

Impact of medical titanium implant surface on the efficiency of fibrointegration

2020 ◽  
pp. 50-55
Author(s):  
T. R. Davydova ◽  
А. I. Shaikhaliev ◽  
D. A. Usatov ◽  
G. A. Gasanov ◽  
R. S. Korgoloev
Keyword(s):  
Surface Roughness ◽  
Titanium Dioxide ◽  
Rough Surface ◽  
Soft Tissues ◽  
Titanium Implant ◽  
Implant Surface ◽  
Anatase Structure ◽  
Bioactive Coating ◽  
Cell Structures ◽  
Titanium Alloy Ti6al4v

The aim of this study was to study the effect of surface branching of titanium endoprostheses on the efficiency of fibrointegration. The object of the study was samples of titanium alloy Ti6Al4V in the form of disks with a diameter of 5 mm and a thickness of 1 mm with various surface treatments: 1) samples with a rough surface after sandblasting; 2) samples with a rough surface after sandblasting with a bioactive coating of titanium dioxide TiO2 with anatase structure. The study of surface roughness was carried out by profilometry. Evaluation of the spreading and proliferation of cells on the surface of test samples, as well as evaluation of the effectiveness of fibrointegration was carried out according to standard methods using scanning electron microscopy. During the experiments, mesinchymal stem cells were sown on test samples and the test samples were introduced into the soft tissues of experimental animals. Based on the results obtained, it was concluded that the technology of forming rough surfaces by sandblasting does not provide high uniformity and reproducibility in the nanometer range and, apparently, another method for obtaining a rough surface should be chosen. The application of a bioactive coating of titanium dioxide TiO2 with the anatase structure to the surface of titanium endoprostheses increases the efficiency of fibrointegration, however, primarily the fibrointegration of titanium endoprostheses depends on their surface roughness, which determines the concentration of cell structures, the intensity of their adhesion and the ability to fibrointegrative process.

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