scholarly journals Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells

2010 ◽  
Vol 208 (2) ◽  
pp. 147-159 ◽  
Author(s):  
T. Ishizuka ◽  
T. Hinata ◽  
Y. Watanabe
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
High Glucose ◽  
Glucose Concentration ◽  
High Glucose Concentration ◽  
Angiogenic Growth Factors

scholarly journals Production of insulin producing cells from cord blood mesenchymal stem cells and their potential in cell therapy

2019 ◽  
Vol 9 (6-s) ◽  
pp. 65-71
Author(s):  
Nihad Elsadig Babiker ◽  
Alsadig Gassoum ◽  
Mohamed Abdelrahman Arbab ◽  
Sawsan Ahmed Hamed ALDeaf ◽  
Imad Fadl-Elmula ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Blood Glucose ◽  
Cord Blood ◽  
High Glucose ◽  
Glucose Concentration ◽  
High Glucose Concentration ◽  
Insulin Producing Cells

Introduction: Mesenchymal stem cells (MSCs) were described as adherent cells with a fibroblast-like appearance, have a great capacity for self-renewal while maintaining their multipotency and differentiation into multiple tissues in vivo and in vitro. Methods: MSCs were isolated from cord blood of Sudanese donors using Ficoll-Hypaque gradient density protocol, and differentiate into β- like cells using 3-step protocol. STZ induced diabetic rats were injected intraperitoneally with the differentiated islet β- like cells and blood glucose levels were monitored for seven days. Results: The adherent cell appeared round and sphere after one-week of incubation, and the fibroblast-like colony was strongly attached after three weeks of seeding. The phenotyping of cells showed positivity for CD13, and negativity for CD34, CD45 and HLADR. MSCs were induced into islet-like cells using a 3-step (15-days) protocol. The differentiated cells showed positive diathizone stain and positive imuno-reactivity to anti-human insulin antibody. Secretion of insulin by insulin-producing cells showed positive result with >3.4 u/ml scale reading in high glucose concentration medium. After one-week of transplantation the level of blood glucose was reduced from 410 to 225 mg/dl in the experimental rat. Conclusion: Human UCB-MSCs can be differentiated into insulin-secreting cells invitro, and are able to produce and secrete insulin in response to high glucose concentration in vivo and in vitro. Keywords: Cord blood, Mesenchymal stem cell, islets β-like cells

scholarly journals Preconditioning of Hypoxic Culture Increases The Therapeutic Potential of Adipose Derived Mesenchymal Stem Cells

2021 ◽  
Vol 9 (F) ◽  
pp. 505-515
Author(s):  
Tito Sumarwoto ◽  
Heri Suroto ◽  
Ferdiansyah Mahyudin ◽  
Dwikora Novembri Utomo ◽  
Romaniyanto Romaniyanto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Chemokine Receptor ◽  
Therapeutic Potential ◽  
Trophic Factors ◽  
Receptor Type ◽  
Angiogenic Growth Factors ◽  
Hypoxic Precondition

Various in vitro preconditioning strategies have been implemented to increase the regenerative capacity of MSCs. Among them are modulation of culture atmosphere (hypoxia or anoxia), three-dimensional culture (3D), addition of trophic factors (in the form of growth factors, cytokines or hormones), lipopolysaccharides, and pharmacological agents. Preconditioning mesenchymal stem cells by culturing them in a hypoxic environment, which resembles the natural oxygen environment of the tissues (1% –7%) and not with standard culture conditions (21%), increases the survival of these cells via Hypoxia Inducible Factor-1α (HIF-1a) and via Akt-dependent mechanisms. In addition, the hypoxic precondition stimulates the secretion of pro-angiogenic growth factors, increases the expression of chemokines SDF-1 (stromal cell-derived factor-1) and its receptor CXCR4 (chemokine receptor type 4) - CXCR7 (chemokine receptor type 7) and increases engraftment of stem cell. This review aims to provide an overview of the preconditioned hypoxic treatment to increase the therapeutic potential of adipose-derived mesenchymal stem cells.  

Abstract 367: Combined Effect of Dinitrophenol Preconditioning and Jagged-1 Over-expression in Mesenchymal Stem Cells Improved Cardiomyogenesis and Angiogenesis in Rat Model of Myocardial Infarction

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Asmat Salim ◽  
Anwar Ali ◽  
Muhammad A Akhter ◽  
Irfan Khan ◽  
Nadia Naeem
Keyword(s):  
Myocardial Infarction ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Histological Examination ◽  
Heart Function ◽  
Cardiac Regeneration ◽  
Angiogenic Factor ◽  
Control Group ◽  
Angiogenic Growth Factors

Cardiac regeneration following myocardial infarction (MI) largely depends on angiogenesis; an important physiological response that allows the heart to recover. Mesenchymal stem cells (MSCs) can be utilized as a source for cardiac regeneration and angiogenesis using various preconditioning strategies. We investigated the role of cardiomyogenic and angiogenic growth factors combined with preconditioning of MSCs using 2, 4, dinitrophenol (DNP). MSCs were isolated from rat bone marrow and treated with DNP; an uncoupler of oxidative phosphorylation. Based on our earlier report in which we showed that DNP treatment affect the expression of cardiomyogenic and angiogenic factors after different re-oxygenation periods, we selected jagged 1 (Jag1), neuropilin1 (Nrp1), TATA box 20 (Tbx20), and myogenin (Myo) to transfect MSCs. Rat MI models were developed through ligation of left anterior descending coronary artery and confirmed through echocardiographic evaluation 4 weeks post MI. The study was divided into different groups which include control; MI model; and MI groups that received untreated, DNP-treated, transfected-MSCs using the above mentioned growth factors and transfected and DNP-treated MSCs. Functional performance of the hearts was analyzed through echocardiography while regeneration of cardiomyocytes and angiogenesis were observed through histological examination. Significant (p<0.05) improvement in the heart function was observed in case of all treatment groups when compared with the normal MSCs. Histological examination of the heart sections 4 weeks post MI showed that MSCs home towards the site of injury. Most prominent result was observed in case of Jag1-transfected and DNP treated MSCs with heart function comparable to that of the control group. Cardiac regeneration and newly developed blood vessels were prominent in the tissue sections. Significant reduction (p<0.05) in the infarct size was also observed as compared to that in case of Myo, Tbx20 and Nrp1 groups. It is, therefore, concluded that Jag1-transfected MSCs can be a suitable angiogenic factor to be used in combination with DNP for preconditioning of MSCs for future regenerative therapy for cardiomyogenesis.

scholarly journals Nature of glycosylphosphatidylinositols produced by mouse embryonic stem cells

Reproduction ◽  
2001 ◽  
pp. 785-791 ◽  
Author(s):  
LR Quinlan ◽  
MT Kane
Keyword(s):  
Signal Transduction ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Glucose ◽  
Glucose Concentration ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
High Glucose Concentration ◽  
Unknown Lipid ◽  
Phosphatidylinositol 4

Incorporation of [(3)H]inositol into mouse embryonic stem cells of the CCE cell line leads to the labelling of the three common phosphoinositides, phosphatidylinositol, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate, and a fourth unknown lipid (lipid X). Incubation with [(3)H]glucosamine results in the labelling of lipid X and at least one other lipid that co-migrates with phosphatidylinositol (lipid Y), indicating that both of these lipids are putative glycosylphosphatidylinositols. In this study, the incorporation of other possible glycosylphosphatidylinositol precursors, ethanolamine, mannose and galactose, into lipids X and Y was examined. Galactose was incorporated into lipids X and Y, and ethanolamine and mannose into lipid Y only. Inhibitors of glycosylphosphatidylinositol biosynthesis pathways, mannosamine and 2-fluoro-2-deoxyglucose, both significantly inhibited ethanolamine incorporation into lipid Y. A high glucose concentration (25 mmol l(-1)) abolished the action of both inhibitors. Phospholipase C treatment of embryonic stem cells that had been labelled in culture with [(3)H]ethanolamine caused a large release of ethanolamine label into the incubation medium and markedly decreased the amount of ethanolamine-labelled lipid Y remaining in the cell membranes. These effects were almost totally abolished by incubation with mannosamine before ethanolamine labelling. These studies strongly indicate that lipid Y is a member of the protein anchor class of glycosylphosphatidylinositol, whereas lipid X is a member of the signal transduction inositol phosphoglycan class of glycosylphosphatidylinositol.

High Glucose Affects the Cytotoxic Potential of Rapamycin, Metformin and Hydrogen Peroxide in Cultured Human Mesenchymal Stem Cells

2019 ◽  
Vol 19 (9) ◽  
pp. 688-698 ◽  
Author(s):  
Azam Roohi ◽  
Mahin Nikougoftar ◽  
Hamed Montazeri ◽  
Shadisadat Navabi ◽  
Fazel Shokri ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
High Glucose ◽  
Cell Cycle Distribution ◽  
Efficient Treatment ◽  
Chronic Hyperglycemia ◽  
Placental Mesenchymal Stem Cells

Background: Oxidative stress and chronic hyperglycemia are two major side effects of type 2 diabetes affecting all cell types including mesenchymal stem cells (MSCs). As a cell therapy choice, understanding the behavior of MSCs will provide crucial information for efficient treatment. Methods: Placental mesenchymal stem cells were treated with various concentrations of glucose, metformin, rapamycin, and hydrogen peroxide to monitor their viability and cell cycle distribution. Cellular viability was examined via the MTT assay. Cell cycle distribution was studied by propidium iodide staining and apoptosis was determined using Annexin Vpropidium iodide staining and flow cytometry. Involvement of potential signaling pathways was evaluated by Western blotting for activation of Akt, P70S6K, and AMPK. Results: The results indicated that high glucose augmented cell viability and reduced metformin toxic potential. However, the hydrogen peroxide and rapamycin toxicities were exacerbated. Conclusion: Our findings suggest that high glucose concentration has a major effect on placental mesenchymal stem cell viability in the presence of rapamycin, metformin and hydrogen peroxide in culture.

scholarly journals Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

2021 ◽  
Vol 22 (9) ◽  
pp. 4604
Author(s):  
Giuliana Mannino ◽  
Anna Longo ◽  
Florinda Gennuso ◽  
Carmelina Daniela Anfuso ◽  
Gabriella Lupo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
High Glucose ◽  
Angiogenic Factors ◽  
Diabetic Patients ◽  
Ros Production ◽  
Migration Ability ◽  
And Migration

A pericyte-like differentiation of human adipose-derived mesenchymal stem cells (ASCs) was tested in in vitro experiments for possible therapeutic applications in cases of diabetic retinopathy (DR) to replace irreversibly lost pericytes. For this purpose, pericyte-like ASCs were obtained after their growth in a specific pericyte medium. They were then cultured in high glucose conditions to mimic the altered microenvironment of a diabetic eye. Several parameters were monitored, especially those particularly affected by disease progression: cell proliferation, viability and migration ability; reactive oxygen species (ROS) production; inflammation-related cytokines and angiogenic factors. Overall, encouraging results were obtained. In fact, even after glucose addition, ASCs pre-cultured in the pericyte medium (pmASCs) showed high proliferation rate, viability and migration ability. A considerable increase in mRNA expression levels of the anti-inflammatory cytokines transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) was observed, associated with reduction in ROS production, and mRNA expression of pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), and angiogenic factors. Finally, a pmASC-induced better organization of tube-like formation by retinal endothelial cells was observed in three-dimensional co-culture. The pericyte-like ASCs obtained in these experiments represent a valuable tool for the treatment of retinal damages occurring in diabetic patients.

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