THE EFFECT OF LARGE DOSES OF THIOUREA ON THE COMPOSITION OF THE LIVER AND URINE OF RATS

1956 ◽  
Vol 13 (2) ◽  
pp. 141-149 ◽  
Author(s):  
F. DOLJANSKI ◽  
Z. ESHKOL ◽  
D. GIVOL ◽  
E. KAUFMANN ◽  
E. MARGOLIASH
Keyword(s):  
Uric Acid ◽  
Urinary Excretion ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Catalase Activity ◽  
Deoxyribonucleic Acid ◽  
Liver Weight ◽  
Liver Glycogen ◽  
Chemical Changes ◽  
Thiourea Treatment

SUMMARY Following the observation that large daily doses of thiourea induced numerous mitoses in the liver of rats [Rachmilewitz, Rosin & Doljanski, 1950], the concomitant chemical changes in the liver and urine of thiourea-treated rats were investigated. It was found that: (1) The mitogenetic reaction, which occurred on the 3rd day of treatment with the drug, was preceded by (a) a decrease in liver catalase activity to 30–50% of the normal within 12 hr; (b) an increase in total liver weight and concentration of liver ribonucleic acid within 2 days. The enlarged liver was normal with respect to protein, lipid, water and deoxyribonucleic acid content. (2) The concentration of liver glycogen decreased markedly on the 1st day of thiourea treatment but returned to normal by the 2nd or 3rd day. (3) During the period of treatment with thiourea there was an increase in the urinary excretion of uric acid, allantoin, and phosphate, as well as glucosuria and polyuria. There was no change in the excretion of urea and total sulphate. (4) All these changes, both in the liver and the urine, were fully reversible on withdrawing the drug.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

EFFECT OF CORTISOL ON GROWTH AND URIC ACID EXCRETION IN THE CHICK

1968 ◽  
Vol 40 (2) ◽  
pp. 145-151 ◽  
Author(s):  
B. M. ADAMS
Keyword(s):  
Uric Acid ◽  
Young Male ◽  
Liver Weight ◽  
Liver Glycogen ◽  
Metabolic Effects ◽  
Acid Excretion ◽  
Uric Acid Excretion ◽  
Temporal Relationships ◽  
Male Chick ◽  
Glycogen Deposition

SUMMARY Metabolic effects of various doses of cortisol were studied in the young male chick. Inhibition of body growth increased with dose but there was no proportional decrease in food intake. Liver weight and uric acid excretion increased similarly with dose. When the temporal relationships of these effects were compared, there was no correlation between the increase in uric acid excretion and the increase in liver weight. It is therefore concluded that the increase in liver weight and uric acid excretion are not linked to a common effect of cortisol. Liver glycogen deposition was more sensitive to cortisol compared with the other effects.

THE INFLUENCE OF GROWTH HORMONE ON THE NUCLEIC ACID CONTENT OF RAT LIVER AND MUSCLE

1956 ◽  
Vol 13 (4) ◽  
pp. 429-432 ◽  
Author(s):  
BARBARA J. GRAY
Keyword(s):  
Skeletal Muscle ◽  
Growth Hormone ◽  
Nucleic Acid ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Female Rats ◽  
Nucleic Acid Content ◽  
Pituitary Growth Hormone

SUMMARY Adult female rats were treated with purified pituitary growth hormone for 9 days, and the nucleic acid content of the liver and skeletal muscle determined. An increase was observed in the concentration of muscle ribonucleic acid, expressed per gram of nitrogen, as a result of the treatment. There were no changes in the ribonucleic acid concentration of liver. No changes were observed in the concentration of deoxyribonucleic acid in muscle or liver.

Composition of Methanospirillum hungatii GP1 during growth on different media

1980 ◽  
Vol 26 (5) ◽  
pp. 577-582 ◽  
Author(s):  
C. Breuil ◽  
G. B. Patel
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Synthetic Medium ◽  
Density Measurement ◽  
Dry Weight ◽  
Weight Basis ◽  
Total Carbohydrate ◽  
Mineral Salts

Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.

Some histochemical changes underlying shoot initiation in tobacco callus cultures

1970 ◽  
Vol 48 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Trevor A. Thorpe ◽  
Toshio Murashige
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Histochemical Study ◽  
Callus Cultures ◽  
Tobacco Callus ◽  
Apparent Change ◽  
Shoot Initiation ◽  
Histochemical Changes

A comparative histochemical study of changes in nucleic acids, proteins, and carbohydrates was performed on freeze-substituted samples of tobacco callus, cultured under shoot-forming and non-organ-forming conditions. The DNA (deoxyribonucleic acid) content per cell showed no apparent change, whereas denser staining for RNA (ribonucleic acid) and protein were observed in shoot-forming regions just before and during the initiation of organized structures. The most dramatic finding was the heavy accumulation of starch in shoot-forming tissue; this accumulation preceded any organ formation and was mainly in regions which ultimately gave rise to primordia.

A Simple Method for measuring Ribonucleic Acid Content of Preparations of Deoxyribonucleic Acid

Nature ◽  
1965 ◽  
Vol 207 (4998) ◽  
pp. 758-759 ◽  
Author(s):  
J. PHILIP SAVITSKY ◽  
FRIEDA STAND
Keyword(s):  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Simple Method

The Protective Efficacy of Ethanolic Leaves Extract of Citronella Gras (Cymbopogon nardus) on Glucose Metabolism Alteration Induced By Mercury in Rats

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Fransisca Diana Alexandra ◽  
Dian Mutiasari ◽  
Trilianty Lestarisa ◽  
Eko Suhartono
Keyword(s):  
Glucose Metabolism ◽  
Protective Effect ◽  
Plant Extract ◽  
Protective Efficacy ◽  
Liver Weight ◽  
Liver Glycogen ◽  
Liver Cells ◽  
Glycogen Level ◽  
Group I ◽  
Citronella Grass

The present study was undertaken to investigate the protective effect of ethanolic citronella grass (C. nardus) leaves extract against mercury (Hg) induced glucose metabolism alteration in rats. Four groups of rats were selected, with 6 rats for each group. Animals of group I was received a 1 ppm of Hg only. Animals of groups II, III, and IV received a combination of 1 ppm Hg and plant extract in different dose (1650, 2520, and 3360 mg/ml). The experiment lasted for 4 weeks. The various parameters studied included liver weight, liver glucose, glycogen, and malondialdehyde (MDA) level in all groups after treatment. The results of this present studies showed that the Hg-induced glucose metabolism alteration in rats which can be seen from the increase of liver glucose and the decreasing of liver glycogen levels. The results also showed that the Hginduced glucose metabolism alteration through its activities in the trigger the liver cells damage which can be seen from the decreasing of liver weight and the increase of liver MDA level. The ethanolic of C. nardus leaves extract shows a protective effect to maintain all parameters into a better a condition which can be seen from the significant increase in liver weight and liver glycogen level, and the significant decrease in liver glucose and MDA levels. The present study indicated that the ethanolic C. nardus leaves extract showed a potential protective effect on glucose metabolism alteration induced by Hg

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