Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig

The metabolic difference in caecal digesta between Jinhua pig and Landrace pig was compared. Twenty weaned piglets at 28 days of age, including ten Landrace pigs (a Western pig breed) and ten Jinhua pigs (a Chinese native pig breed), were randomly selected and allocated into two groups. The pigs were fed the same corn-soybean diet on the same pig farm. At the age of 240 days, all pigs of each group were slaughtered, the digesta in the caecum of the twenty pigs were collected for metabolomic analysis and determination of short-chain fatty acids (SCFAs). The results showed that a total of 56 different metabolites (22 metabolites named and 34 metabolites without identification) were detected in caecal digesta using a gas chromatography time-of-flight/mass spectrometry (GC-TOF-MS)-based metabolomic approach. Forty-six of the 56 metabolites were upregulated significantly (P < 0.05) in Landrace group compared with Jinhua group. The metabolic pathways with different impact value in which different metabolites were mainly involved were tyrosine metabolism, citrate cycle and steroid biosynthesis. In addition, we found that Landrace accumulated more SCFAs in caecal digesta, while the concentrations of acetic acid (P < 0.01) and butyric acid (P < 0.05) in caecal digesta of Jinhua pig were markedly lower than those of Landrace pig. Collectively, our study was the first to compare the metabolic difference in caecal digesta between Jinhua pig and Landrace pig using a metabolomics approach, which might be used as a potential metabolomics mechanism to research different breeds of pigs.

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Plastics - Determination of the molecular mass and molecular mass distribution of polymer species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)

10.3403/30350552 ◽

2018 ◽

Keyword(s):

Molecular Mass ◽

Molecular Mass Distribution ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Flight Mass Spectrometry ◽

Polymer Species ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Simultaneous determination of multi-classes of veterinary drug residues in pork by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2015.04033 ◽

2015 ◽

Vol 33 (9) ◽

pp. 1002 ◽

Cited By ~ 1

Author(s):

Wanyan ZHU ◽

Xin ZHANG ◽

Juan YANG ◽

Wenyuan XU ◽

Meiling XU

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Simultaneous Determination ◽

Time Of Flight ◽

Ultra Performance Liquid Chromatography ◽

Veterinary Drug ◽

Veterinary Drug Residues ◽

Drug Residues ◽

Flight Mass Spectrometry

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Determination of congo red in beef by high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry and ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2013.03043 ◽

2013 ◽

Vol 31 (9) ◽

pp. 914 ◽

Cited By ~ 2

Author(s):

Hui LIN ◽

Chunxiang XU ◽

Chunrong YAN ◽

Zheng ZHANG ◽

Suilou WANG

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Congo Red ◽

High Performance ◽

Triple Quadrupole ◽

Quadrupole Mass ◽

Flight Mass Spectrometry ◽

Tandem Quadrupole

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Metabolomics and DNA-Based Authentication of Two Traditional Asian Medicinal and Aromatic Species of Salvia subg. Perovskia

Cells ◽

10.3390/cells10010112 ◽

2021 ◽

Vol 10 (1) ◽

pp. 112

Author(s):

Monika Bielecka ◽

Bartosz Pencakowski ◽

Marta Stafiniak ◽

Klemens Jakubowski ◽

Mehdi Rahimmalek ◽

...

Keyword(s):

Dna Barcoding ◽

High Performance ◽

Carnosic Acid ◽

Limited Information ◽

Metabolomic Analysis ◽

Separate Species ◽

Flight Mass Spectrometry ◽

Aerial Parts ◽

Central Asian ◽

Aromatic Species

Subgenus Perovskia of the extended genus of Salvia comprises several Central Asian medicinal and aromatic species, of which S. yangii and S. abrotanoides are the most widespread. These plants are cultivated in Europe as robust ornamentals, and several cultivars are available. However, their medicinal potential remains underutilized because of limited information about their phytochemical and genetic diversity. Thus, we combined an ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS) based metabolomics with DNA barcoding approach based on trnH-psbA and ITS2 barcodes to clarify the relationships between these two taxa. Metabolomic analysis demonstrated that aerial parts are more similar than roots and none of the major compounds stand out as distinct. Sugiol in S. yangii leaves and carnosic acid quinone in S. abrotanoides were mostly responsible for their chemical differentiation, whereas in roots the distinction was supported by the presence of five norditerpenoids in S. yangii and two flavonoids and one norditerpenoid in S. abrotanoides. To verify the metabolomics-based differentiation, we performed DNA authentication that revealed S. yangii and S. abrotanoides to be very closely related but separate species. We demonstrated that DNA barcoding coupled with parallel LC-MS profiling constitutes a powerful tool in identification of taxonomically close Salvia species.

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Determination of Bioactive Markers for the Discrimination of Syneilesis palmata and Paris verticillata by High Performance Liquid Chromatography (HPLC) with Diode Array Detection (DAD) and Ion Trap Time-of-Flight Mass Spectrometry (IT-TOF-MS)

Analytical Letters ◽

10.1080/00032719.2021.1878527 ◽

2021 ◽

pp. 1-10

Author(s):

Ji Young Jeong ◽

Md Atikul Islam ◽

Joon Ho Hong ◽

Ji Hyeon Son ◽

Ok Yeon Song ◽

...

Keyword(s):

Mass Spectrometry ◽

High Performance ◽

Ion Trap ◽

Diode Array ◽

Diode Array Detection ◽

Flight Mass Spectrometry ◽

Syneilesis Palmata ◽

Array Detection ◽

Tof Ms

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Simultaneous determination of various platycosides in Four Platycodon grandiflorum cultivars by UPLC-QTOF/MS

Applied Biological Chemistry ◽

10.1186/s13765-019-0457-x ◽

2019 ◽

Vol 62 (1) ◽

Author(s):

Dae Young Lee ◽

Bo-Ram Choi ◽

Jae Won Lee ◽

Yurry Um ◽

Dahye Yoon ◽

...

Keyword(s):

Principal Component ◽

Ultra Performance Liquid Chromatography ◽

Platycodon Grandiflorum ◽

Pentacyclic Triterpenoid ◽

Flight Mass Spectrometry ◽

Platycodi Radix ◽

Isomeric Pairs ◽

Different Levels

Abstract In Platycodi Radix (root of Platycodon grandiflorum), there are a number of platycosides that consist of a pentacyclic triterpenoid aglycone and two sugar moieties. Due to the pharmacological activities of platycosides, it is critical to assess their contents in PR, and develop an effective method to profile various platycosides is required. In this study, an analytical method based on ultra performance liquid chromatography coupled with quadrupole time-of-flight/mass spectrometry (UPLC-QTOF/MS) with an in-house library was developed and applied to profile various platycosides from four different Platycodi Radix cultivars. As a result, platycosides, including six isomeric pairs, were successfully analyzed in the PRs. In the principal component analysis, several platycosides were represented as main variables to differentiate the four Platycodi Radix cultivars. Their different levels of platycosides were also represented by relative quantification. Finally, this study indicated the proposed method based on the UPLC-QTOF/MS can be an effective tool for identifying the detail characterization of various platycosides in the Platycodi Radix.

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