scholarly journals Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR

2019 ◽  
Vol 56 (No. 1) ◽  
pp. 9-14 ◽  
Author(s):  
Jana Palicová ◽  
Pavel Matušinsky ◽  
Alena Hanzalová ◽  
Ivana Svačinová ◽  
Veronika Dumalasová ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Real Time ◽  
Resistance Gene ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Winter Wheat Cultivars ◽  
Small Plot ◽  
Low Levels ◽  
Oculimacula Yallundae ◽  
Pathogen Dna

The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017–2018. The assessment was carried out visually by symptoms and by a quantitative real-time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues followed a moderate linear regression. Low levels of eyespot were observed in the cultivars/lines possessing the resistance gene Pch1 (Annie, Hermann, Rebell, SG-S1215-14, SG-S1825-14, SG-S791-13) and also in the line SG-SU630-15. The qPCR method was able to detect low levels of the pathogens in the plant tissue and to distinguish two eyespot pathogens. O. acuformis was detected in very low concentrations in the inoculated plants compared with O. yallundae. The eyespot infection rate was significantly higher in 2017 than in the next agricultural season due to extremely dry and warm spring weather in 2018.

scholarly journals Resistance of winter wheat cultivars to eyespot and characterisation of causal agents of the disease

2017 ◽  
Vol 54 (No. 1) ◽  
pp. 24-30
Author(s):  
Palicová Jana ◽  
Matušinsky Pavel ◽  
Dumalasová Veronika ◽  
Hanzalová Alena ◽  
Bížová Irena
Keyword(s):  
Czech Republic ◽  
Winter Wheat ◽  
Natural Occurrence ◽  
Wheat Cultivars ◽  
The Czech Republic ◽  
Stem Base ◽  
Winter Wheat Cultivars ◽  
Lower Severity ◽  
Small Plot ◽  
Oculimacula Yallundae

The reaction of ten winter wheat cultivars grown in the Czech Republic to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in a small plot trial. In a parallel field trial the natural occurrence of stem-base disease complex in six of the tested cultivars was assessed. Lower severity of eyespot (and/or stem-base diseases) was observed in cultivars possessing the resistance gene Pch1 (cvs Hermann, Annie, Princeps, Manager, and Rebell) in plots inoculated with Oculimacula spp. as well as in natural field conditions. A total of 468 wheat stem bases from the Czech Republic was screened by PCR to study the frequency of eyespot causal agents. The plants were colonised significantly more often by both species Oculimacula yallundae and O. acuformis together than separately.

scholarly journals Reaction of winter wheat genotypes to infection by Mycosphaerella graminicola(Fuckel) Schroeter

2013 ◽  
Vol 55 (1) ◽  
pp. 233-246
Author(s):  
Ewa Mirzwa-Mróz ◽  
Czesław Zamorski
Keyword(s):  
Winter Wheat ◽  
Broad Spectrum ◽  
Mycosphaerella Graminicola ◽  
Wheat Breeding ◽  
Septoria Tritici ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Wheat Genotypes ◽  
Winter Wheat Cultivars

The response of Polish winter wheat genotypes to <i>M.graminicola</i> (preliminary experiments and cultivar collections) was observed in different regions of Poland. Observations were carried out in 1995-1999. The winter wheat genotypes showed a broad spectrum of reaction to this pathogen. Between 1997 and 1999 the highest degree of infection on winter wheat breeding lines was noted in Kończewice. During this time no genotypes free from infection were observed (preliminary breeding experiments). Cultivars with no symptoms of <i>Septoria tritici</i> blotch (Leszczyńska Wczesna and Żelazna) were found among old genotypes in Słupia Wielka only in earlier experiments (1995-1996). In the years 1997-1999 the winter wheat cultivars were classified into groups on the basis of their response to the pathogen. The degree of infection for the majority cultivars was quite high.

scholarly journals Reactions of winter wheat cultivars to Septoria nodorum Berk.

1989 ◽  
Vol 61 (5) ◽  
pp. 379-385
Author(s):  
Pirjo Peltonen ◽  
Sinikka Karjalainen ◽  
Reijo Karjalainen
Keyword(s):  
Winter Wheat ◽  
Flag Leaf ◽  
Adult Plant ◽  
Lesion Length ◽  
Septoria Nodorum ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Seedling Plant ◽  
Plant Stage ◽  
Winter Wheat Cultivars

Twelve Finnish and foreign winter wheat cultivars and breeding lines were evaluated for resistance to Septoria nodorum at seedling and adult plant stage. Flag leaf severity varied between 6.5 and 20 % and ear severity between 2.5 and 10 %. In general, Cl 13091 and Skjaldar were the most resistant cultivars, while Hja 21614, Hja 21638, and Hankkijan Ilves were the most susceptible ones. Aura and Mironovskaja 808 were most resistant to ear infection, while Vakka and Hja 21638 were most susceptible. Assessment of susceptibility at the seedling stage was quite well correlated with ratings of adult plants in the field (r = 0.72). The correlation between lesion length and seedling plant Septoria severity was moderate (r = 0.68). Selection of wheat lines for Septoria resistance is discussed.

scholarly journals Reaction of winter wheat cultivars and breeding lines to Blumeria graminis f.sp. tritici

2010 ◽  
Vol 42 (No. 1) ◽  
pp. 15-20 ◽  
Author(s):  
L. Věchet
Keyword(s):  
Powdery Mildew ◽  
Winter Wheat ◽  
Disease Severity ◽  
Infection Type ◽  
Specific Gene ◽  
Powdery Mildew Fungus ◽  
Breeding Lines ◽  
Resistant Cultivars ◽  
Winter Wheat Cultivars ◽  
Small Plot

During 4 years, 27 cultivars and breeding lines of winter wheat (<i>Triticum aestivum</i>) were tested in small plot experiments for resistance to powdery mildew fungus. The most resistant were Frimegu, RE9607, Runal, Asset, Folke and Wasmo. The cultivars Asta (<i>Pm</i>2,6) and Vlasta (<i>Pm</i>2,6 and another not determined specific gene or minor genes of resistance) fall into resistant cultivars. It seems that the specific genes of resistance <i>Pm</i>2 and <i>Pm</i>6 are still very effective against the present Czech population of powdery mildew on wheat. Resistance of the cultivars Hereward and Tarso, having the gene of resistance <i>Pm</i>8, can be ascribed to an additional undetermined gene that is effective only in mature plants. The cultivars Mikon and Ramiro with partial resistance had a higher infection type and disease severity than resistant cultivars, but lower disease severity than the susceptible cultivar Kanzler.

scholarly journals Occurrence of Septoria tritici blotch in 1994-1996

2013 ◽  
Vol 50 (1-2) ◽  
pp. 11-26
Author(s):  
Czesław Zamorski ◽  
Bogdan Nowicki ◽  
Ewa Mirzwa-Mróz
Keyword(s):  
Winter Wheat ◽  
Weather Condition ◽  
Mycosphaerella Graminicola ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
High Infection ◽  
Winter Wheat Cultivars ◽  
Very High

The aim of the work was to study an occurrence and intensity of septoria tritici blotch on wheat in different regions of Poland as well as reaction of winter wheat cultivars and breeding lines to infection by <em>Mycosphaerella graminicola</em> (Fuckel) Schroeter (an. <em>Septoria tritici</em> ( Rob. ex Desm .) under field conditions. Observation conducted during 1994-1996 indicated on occurrence of septoria tritici blotch each year and on important infection degree of winter wheat by <em>Mycosphaerella gruminicola</em> in some regions of the country. The highest infection was noted in 1995 and the lowest in 1994. Infection degree for majority of cultivars was quite high. Some cultivars and lines were characterized by very high infection. Among them were the old (Kujawianka Więcławska and Wysokolitewka Sztywnosłoma) and the newest (Tercja) cultivars. On some old cultivars (Leszczyńska Wczesna and Żelazna) even no traces of infection were observed although favourable weather condition existance and the presence of neighbourhood diseased plants. Field observation of cultivars and breeding lines showed different reaction of tested wheat genotypes to <em>Mycosphaerella graminicola</em> infection.

scholarly journals Selenium in colour-grained winter wheat and spring tritordeum

2017 ◽  
Vol 63 (No. 7) ◽  
pp. 315-321
Author(s):  
Phuong LE Minh ◽  
Lachman Jaromír ◽  
KOTÍKOVÁ Zora ◽  
Orsák Matyáš ◽  
MICHLOVÁ Tereza ◽  
...  
Keyword(s):  
Czech Republic ◽  
Winter Wheat ◽  
Dry Matter ◽  
Breeding Line ◽  
Wheat Cultivars ◽  
Crop Season ◽  
Breeding Lines ◽  
Genetic Potential ◽  
Winter Wheat Cultivars ◽  
Grain Colour

Eighteen winter wheat cultivars with different grain colour (purple-, blue-, yellow- and red-grained) and three spring tritordeum yellow-grained cultivars and breeding lines were assessed for grain selenium (Se) content from the crop season 2014/2015 on the experimental field Agrotest fyto, Ltd., Kroměříž (Czech Republic). Se content has shown to be genotype dependent, with the highest contents in control red-grained cv. Bohemia (0.235 mg/kg dry matter (DM)) and yellow-grained cv. Bona Vita (0.229 mg/kg DM), and breeding lines V2 10–16 (blue-grained), KM 53–14 (blue-grained) and V2 15–16 (yellow-grained) winter wheats. In new spring tritordeums, average Se content was comparable (0.039 mg/kg DM) with purple pericarp wheats (0.042 mg/kg DM); in wheats with blue aleurone and yellow endosperm it was higher (0.057 mg/kg DM and 0.069 mg/kg DM). Although in most cultivars the Se contents were not significantly different, statistically significant differences were determined between the cvs. Bohemia and Bona Vita with the highest Se content and breeding line V2 31–16 with the lowest Se content as well as between the cv. Bohemia and breeding line KM 178–14. Grain colour of wheat cultivars and breeding lines affected Se content, so possible wheat genetic resources for use in the breeding process can be assessed. Diversity in certain wheat accessions offers genetic potential for developing cultivars with better ability to accumulate beneficial Se micronutrient in grains.

scholarly journals Reaction of 50 Winter Wheat Cultivars Grown in the Czech   Republic to Pyrenophora tritici-repentis Races 1, 3, and 6

2011 ◽  
Vol 42 (No. 2) ◽  
pp. 31-37
Author(s):  
J. Palicová-Šárová ◽  
A. Hanzalová
Keyword(s):  
Czech Republic ◽  
Winter Wheat ◽  
Moderate Level ◽  
Wheat Cultivars ◽  
Artificial Infection ◽  
The Czech Republic ◽  
Breeding Lines ◽  
Pyrenophora Tritici Repentis ◽  
Winter Wheat Cultivars ◽  
High Level

The reaction of 50 winter wheat cultivars/lines to artificial infection with Pyrenophora tritici-repentis (PTR) races 1, 3, and 6 was studied under greenhouse conditions. The set of tested cultivars/lines included predominantly cultivars registered in the Czech Republic and some new breeding lines. A high level of resistance to P. tritici-repentis was detected in the cultivars Clarus, Rheia, Cubus, SHMK WW 14-92, &Scaron;&aacute;rka, Vlasta and Dromos (SWS 799.14953), susceptible reactions were observed in the cultivars Caphorn, Corsaire, Karolinum, Heroldo (PBIS 00/91), Hedvika, Biscay, Svitava, Barroko (PBIS 00/140) to all three races tested. The majority of the tested cultivars possess a moderate level of resistance to PTRraces 1, 3, and 6. Significant differences were proved not only in the reaction of the tested cultivars but also in the aggressiveness of the three used isolates. &nbsp;

Sign in / Sign up

Export Citation Format

Share Document