scholarly journals Co-infection with papillomavirus and Knemidokoptes jamaicensis (Acari: Knemidokoptidae) in a chaffinch (Fringilla coelebs) and a case of beak papillomatosis in another chaffinch

2012 ◽  
Vol 50 (No. 6) ◽  
pp. 276-280 ◽  
Author(s):  
I. Literak ◽  
B. Smid ◽  
F. Dusbabek ◽  
R. Halouzka ◽  
L. Novotny
Keyword(s):  
Czech Republic ◽  
Histopathological Examination ◽  
Electron Microscopic Examination ◽  
Skin Lesions ◽  
Intranuclear Inclusions ◽  
Stratum Granulosum ◽  
Electron Microscopic ◽  
The Czech Republic ◽  
Fringilla Coelebs ◽  
Viral Aetiology

Nodular papillomatous lesions combined with proliferation and formation of scales and crusts were observed on both tarsi of a dead chaffinch (Fringilla coelebs) found in February 2004 in the eastern part of the Czech Republic. Electron microscopic examination demonstrated the presence of papillomavirus virions in the lesions. The parasitological examination of crust scraps revealed the presence of mites Knemidokoptes jamaicensis. Skin lesions were caused by a co-infection with the two pathogens. This paper is the first report of such a co-infection. In another chaffinch netted in May 2004 in the northern part of the Czech Republic, marked papillomatous lesions were found at the base of the beak. Proliferation of basal epidermal cells with sporadic intranuclear inclusions in the keratinocytes of stratum granulosum of the epidermis observed in the histopathological examination was suggestive of a viral aetiology of the papillomatous lesions. This is the first reported case of beak papillomatosis in the chaffinch.

scholarly journals Papillomatosis in chaffinches (Fringilla coelebs) in the Czech Republic and Germany

2012 ◽  
Vol 48 (No. 6) ◽  
pp. 169-174 ◽  
Author(s):  
I. Literák ◽  
B. Šmíd ◽  
L. Valíček
Keyword(s):  
Electron Microscopy ◽  
Czech Republic ◽  
Staining Technique ◽  
Skin Lesions ◽  
Negative Staining ◽  
The Czech Republic ◽  
First Report ◽  
Fringilla Coelebs ◽  
Direct Observations

Analyses of ornithological field records, direct observations of typical lesions, and examination by electron microscopy revealed leg papillomatosis in six chaffinches (Fringilla coelebs) in theCzech Republic and one chaffinch inGermany. Papillomavirus was identified by electron microscopy using the negative staining technique in skin lesions of one chaffinch caught in theCzechRepublic. This is the first report of papillomatosis in chaffinches recorded in theCzechRepublic.

scholarly journals The occurrence of dermatosparaxis in a commercial Drakensberger cattle herd in South Africa : article

2008 ◽  
Vol 79 (1) ◽  
Author(s):  
D.E. Holm ◽  
E. Van Wilpe ◽  
C.K. Harper ◽  
N.M. Duncan
Keyword(s):  
South Africa ◽  
Clinical Signs ◽  
Size Variation ◽  
Electron Microscopic Examination ◽  
Skin Lesions ◽  
Detailed Examination ◽  
Electron Microscopic ◽  
Loose Packing ◽  
Dermal Collagen ◽  
Skin Abnormalities

Dermatosparaxis is a heritable collagen dysplasia causing skin extensibility and fragility. In Belgian Blue cattle this mutation has been described as a 3 base pair (bp) change followed by a 17 bp deletion in the gene coding for procollagen 1 N-Proteinase (pNPI). An outbreak in a commercial Drakensberger herd in South Africa followed the introduction in late 2000 of a 3-year-old bull that developed skin lesions in 2001 and was culled in 2002. Some of his offspring were similarly affected, 1 of which was kept as a breeding bull after his sire's death. Two affected calves were referred to the Onderstepoort Veterinary Academic Hospital in October 2005. Detailed examination revealed only skin abnormalities limited to the lateral extremities of the thorax, abdomen and pelvis, viz. either acute lacerations of varying sizes, slow healing defects or thin scars in chronic cases. During a subsequent farm visit, 13 animals with similar wounds were seen in the herd of 146 animals. Electron microscopic examination of skin biopsies revealed haphazard arrangement and loose packing of dermal collagen fibrils within collagen fibres. The fibrils showed size variation and slightly irregular outlines on cross-section, consistent with mild dermatosparaxis. DNA samples of affected calves were analysed using primers designed to amplify the region of the pNPI gene that contained the mutation described in Belgian Blue cattle, but this mutation could not be demonstrated in any of the animals tested. It is concluded that a form of dermatosparaxis with a different gene mutation from that described in Belgian Blue cattle exists in Drakensberger cattle in South Africa. This possibly also explains the milder and more delayed clinical signs and the milder dermal collagen ultrastructural abnormalities.

Persistent papillomavirus infection in a cat

1996 ◽  
Vol 32 (5) ◽  
pp. 392-396 ◽  
Author(s):  
F Lozano-Alarcon ◽  
Lewis TP2nd ◽  
EG Clark ◽  
GA Bradley ◽  
MR Shupe ◽  
...  
Keyword(s):  
Chronic Pancreatitis ◽  
Clinical Diagnosis ◽  
Skin Lesions ◽  
Stratum Granulosum ◽  
Electron Microscopic ◽  
Papillomavirus Infection

A seven-year-old, neutered male, crossbred Persian was diagnosed as having persistent, cutaneous papillomavirus infection. The skin lesions consisted of round, multifocal-to-confluent, raised, black plaques on the neck, thorax, shoulders, and forelegs. Papillomavirus virions were demonstrated in negative-stained, electron microscopic preparations of homogenized skin lesions and within the nuclei of cells from the stratum granulosum. Avidin-biotin immunoperoxidase stains were positive for papillomavirus in the same cells. The cat was euthanized due to a clinical diagnosis o concurrent, severe, chronic pancreatitis.

scholarly journals Carvedilol Ameliorates Early Diabetic Nephropathy in Streptozotocin-Induced Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mohamed A. Morsy ◽  
Salwa A. Ibrahim ◽  
Entesar F. Amin ◽  
Maha Y. Kamel ◽  
Soha A. Abdelwahab ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Protective Effect ◽  
Histopathological Examination ◽  
Electron Microscopic Examination ◽  
Diabetic Rats ◽  
Electron Microscopic ◽  
Podocyte Injury ◽  
Stage Renal Disease ◽  
End Stage ◽  
Early Diabetic Nephropathy

Diabetic nephropathy results in end-stage renal disease. On the other hand, carvedilol has been reported to have various pharmacological properties. The aim of this study therefore is to evaluate the possible protective effect of carvedilol on streptozotocin-induced early diabetic nephropathy and various mechanisms underlie this effect in rats. Single i.p. injection of streptozotocin (65 mg/kg) was administered to induce early diabetic nephropathy in Wistar rats. Oral administration of carvedilol at a dose level of 1 and 10 mg/kg daily for 4 weeks resulted in nephroprotective effect as evident by significant decrease in serum creatinine level, urinary albumin/creatinine ratio, and kidney index as well as renal levels of malondialdehyde, nitric oxide, tumor necrosis factor-α, and cyclooxygenase-2 with a concurrent increase in creatinine clearance and renal reduced glutathione level compared to diabetic untreated rats. The protective effect of carvedilol was confirmed by renal histopathological examination. The electron microscopic examination indicated that carvedilol could effectively ameliorate glomerular basement membrane thickening and podocyte injury. In conclusion, carvedilol protects rats against streptozotocin-induced early diabetic nephropathy possibly, in part, through its antioxidant as well as anti-inflammatory activities, and ameliorating podocyte injury.

A dermatosis resembling juvenile cellulitis in an adult dog

1995 ◽  
Vol 31 (3) ◽  
pp. 204-208 ◽  
Author(s):  
JG Jeffers ◽  
DD Duclos ◽  
MH Goldschmidt
Keyword(s):  
Lymph Node ◽  
Microscopic Examination ◽  
Otitis Externa ◽  
Histopathological Examination ◽  
Electron Microscopic Examination ◽  
Acute Onset ◽  
Adult Case ◽  
Electron Microscopic

A two-year-old, female Lhasa apso presented with an acute onset of fever, anorexia, lethargy, prescapular and mandibular lymphadenopathy, otitis externa, and a dermatitis involving the perioral and auricular skin. Histopathological examination of affected skin and a mandibular lymph node was diagnostic for juvenile cellulitis. Extensive hematological, serological, urine, skin, and fecal testing together with special staining, immunofluorescence, and electron microscopic examination of skin and lymph node biopsies failed to reveal an underlying etiology. After 15 weeks the condition resolved completely. This represents the first adult case of a dermatosis fitting the clinical, histological, and clinicopathological description ascribed to juvenile cellulitis.

scholarly journals A novel poxvirus lethal to red squirrels (Sciurus vulgaris)

2003 ◽  
Vol 84 (12) ◽  
pp. 3337-3341 ◽  
Author(s):  
Kathryn Thomas ◽  
Daniel M. Tompkins ◽  
Anthony W. Sainsbury ◽  
Ann R. Wood ◽  
Robert Dalziel ◽  
...  
Keyword(s):  
Sequence Data ◽  
Electron Microscopic Examination ◽  
Skin Lesions ◽  
Comparative Sequence Analysis ◽  
Red Squirrel ◽  
Electron Microscopic ◽  
Red Squirrels ◽  
North East ◽  
The North ◽  
The United Kingdom

A parapoxvirus has been implicated in the decline of the red squirrel in the United Kingdom. Virus was isolated from an outbreak of lethal disease in red squirrels in the north-east of England. Experimental infection of captive-bred red squirrels confirmed that this virus was the cause of the severe skin lesions observed. Electron microscopic examination of the virus showed that it had a morphology typical of parapoxviruses whilst preliminary sequence data suggested a genomic G+C composition of approximately 66 %, again similar to that found in other parapoxviruses. However Southern hybridization analysis failed to detect three known parapoxvirus genes, two of which have been found so far only in the genus Parapoxvirus. Comparative sequence analysis of two other genes, conserved across the eight recognized chordopoxvirus genera, suggests that the squirrel virus represents a previously unrecognized genus of the Chordopoxviridae.

scholarly journals Morphological Changes in the Ligamentum Flavum in Degenerative Lumbar Canal Stenosis: A Prospective, Comparative Study

2020 ◽  
Vol 14 (6) ◽  
pp. 773-781
Author(s):  
Devanand Hulmani ◽  
Bhavuk Garg ◽  
Nishank Mehta ◽  
Asit Ranjan Mridha ◽  
Tapas Chandra Nag ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Morphological Changes ◽  
Electron Microscopic Examination ◽  
Elastic Fibers ◽  
Electron Microscopic ◽  
Canal Stenosis ◽  
Chondroid Metaplasia ◽  
Group 2 ◽  
Group 1

Study Design: Prospective, comparative.Purpose: To compare the histopathological and electron microscopic changes in the ligamentum flavum (LF) between degenerative lumbar canal stenosis (LCS) and lumbar disk herniation (LDH).Overview of Literature: The LF has been implicated as a key structure in the pathogenesis of LCS. With aging, the LF undergoes morphological changes–a decrease in the elastic component and an increase in the collagen component, in addition to other focal changes. By comparing the histopathological and electron microscopic picture of the LF in elderly patients with LCS with that in young patients with LDH, the role of this ligament in the pathogenesis of LCS may be clarified.Methods: Forty patients were prospectively recruited and divided into two groups: group 1 included 20 patients with degenerative LCS aged >55 years and group 2 included patients with LDH aged <35 years. The ligament flava were collected during the patients’ surgery. The features noted on histopathological examination included the fibrosis score, the loss of elastic fibers, calcification, chondroid metaplasia, mucinous degeneration, vascularization, long septa, clefts, granulation tissue, and ganglion-like cysts. The features noted on electron microscopic examination included the elastic fiber thickness, the quality of elastic fibers, the elastic:collagen ratio, calcification, melanin fibers, remnants of necrotic cells, and electron-dense material in the LF. All parameters were compared between group 1 and group 2.Results: On histopathological examination, the two groups exhibited significant differences regarding three parameters: chondroid metaplasia, long septa, and ganglion-like cysts. On electron microscopy examination, significant differences were observed between the two groups regarding two parameters: the quality of elastic fibers and the elastic:collagen ratio.Conclusions: Characteristic morphological changes may be noted on histopathological and electron microscopic examination that mark the degenerative changes in the LF that contribute to the occurrence and pathogenesis of degenerative LCS.

scholarly journals First isolation of Mycobacterium genavense in a blue headed parrot (Pionus menstruus) imported from Surinam (South America) to the Czech Republic: a case report

2010 ◽  
Vol 55 (No. 7) ◽  
pp. 339-347 ◽  
Author(s):  
JE Shitaye ◽  
R. Halouzka ◽  
J. Svobodova ◽  
V. Grymova ◽  
M. Grym ◽  
...  
Keyword(s):  
Czech Republic ◽  
South America ◽  
Inflammatory Reaction ◽  
Culture System ◽  
Liquid Culture ◽  
Histopathological Examination ◽  
Antibody Detection ◽  
Infected Bird ◽  
The Czech Republic ◽  
Mycobacterium Genavense

In 2005 a Mycobacterium genavense infection was diagnosed in one blue-headed parrot (Pionus menstruus) imported from Surinam (South America), the first such incidence in the Czech Republic. The bird died suddenly. Histopathological examination revealed a diffuse granulomatous inflammatory reaction in the intestinal mucosa. Tissue sections of the intestines stained by Ziehl-Neelsen were microscopically positive for acid-fast bacilli. Culture examinations were made by the conventional solid media, the manual BD-BBL MGIT and the automated MGIT 960 liquid culture systems. We have detected mycobacteria by the automated MGIT 960 liquid culture system in the intestinal tissues after 92 days and on solid Herrold's Egg Yolk Medium without Mycobactin J after 270 days. The manual BD-BBL MGIT liquid culture and the conventional culture system revealed mycobacteria after 150 days of cultivation from three and two tissues samples, respectively. M. genavense was identified by HAIN Life Science kits (GmbH, Germany) and was found to be the cause of death for the parrot, which was one of 14 exotic birds kept by its keeper. From the environment M. a. hominissuis was isolated only, which was documented by antibody detection of this member of the M. avium complex in sera samples of five birds. Three two month old common pet parakeets (Melopsittacus undulatus) were for one day in contact with drinking water contaminated by the liver suspension from the infected bird. After 12 months they were euthanized. Subsequent culture was negative for the presence of mycobacteria and histopathological examinations showed no granulomatous inflammatory reaction or any other pathological findings.

Gastric Adenocarcinoma Presenting as an Eyelid and Conjunctival Mass

1992 ◽  
Vol 2 (1) ◽  
pp. 3-9 ◽  
Author(s):  
M. Küchle ◽  
L. Holbach ◽  
U. Schlötzer-Schrehardt
Keyword(s):  
Tumor Cells ◽  
Histopathological Examination ◽  
Electron Microscopic Examination ◽  
Secretory Activity ◽  
Signet Ring Cell Carcinoma ◽  
Signet Ring Cell ◽  
Secondary Malignancy ◽  
Bulbar Conjunctiva ◽  
Electron Microscopic ◽  
Upper Lid

A 50-year-old white man complained of “inflammation” of his right eyelid since January 1989. In June 1990, he had undergone a gastrectomy for an adenocarcinoma of the stomach. In August 1990, his right eye showed a painless, firm infiltration of the upper and lower lid, ulceration and loss of eyelashes of the upper lid and a reddish, subepithelial thickening of the medial bulbar conjunctiva. Biopsies of the right upper lid and nasal bulbar conjunctiva disclosed a metastatic, poorly differentiated adenocarcinoma of the stomach (signet ring cell carcinoma) both in the lid and conjunctival biopsies. Carcinoembryonic antigen (CEA) was detected in the tumor cells. Electron microscopic examination revealed tumor cells with signs of secretory activity. Although metastases to the eyelids and conjunctiva are rare, they may precede the clinical manifestation of the primary tumor for months or even years. In patients with chronic, recalcitrant lesions of eyelids or conjunctiva, especially if accompanied by loss of eyelashes, a primary or secondary malignancy should be excluded by early biopsies and histopathological examination.

Intrathecal Paraganglioma of the Cauda Equina

Neurosurgery ◽  
1984 ◽  
Vol 15 (4) ◽  
pp. 578-582 ◽  
Author(s):  
Marcelino G. Reyes ◽  
Hernando Torres
Keyword(s):  
Chronic Pain ◽  
Histopathological Examination ◽  
Obese Woman ◽  
Cauda Equina ◽  
Lumbar Vertebrae ◽  
Electron Microscopic Examination ◽  
Filum Terminale ◽  
Electron Microscopic ◽  
Lower Back ◽  
Dense Core Granules

Abstract A 33-year-old obese woman with chronic pain in her lower back presented with weakness of her right quadriceps and diminution of her patellar reflex. Her myelogram revealed an intradural oval mass at level of the interspace between her 3rd and 4th lumbar vertebrae. Operation disclosed an encapsulated mass attached to her filum terminale. Histopathological examination showed a paraganglioma with typical dense core granules, confirmed by electron microscopic examination. We report our patient to call attention to intrathecal paragangliomas as a rare and treatable cause of the cauda equina syndrome.

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