scholarly journals Antioxidant activity of phenolic fraction of pea (Pisum sativum)

2013 ◽  
Vol 19 (No. 4) ◽  
pp. 139-142 ◽  
Author(s):  
R. Amarowicz ◽  
M. Karamać ◽  
S. Weidner
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Beta Carotene ◽  
Uv Spectra ◽  
Model System ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Phenolic Fraction

An extract of seeds of pea was prepared using 80% (v/v) acetone. Six fractions (I–VI) were separated from the crude extract on a Sephadex LH-20 column using methanol as the mobile phase. The antioxidant activity of fractions was investigated in a β-carotene-linoleate model system. For individual fractions, UV spectra were recorded and the content of total phenolics was determined. Results of the β-carotene-linoleate model system indicated that antioxidant activities of separated fractions correlated with their content of total phenolic compounds and they decreased in the order of IV = VI > V > III > I > II. The antioxidant activity of fractions IV and VI was very strong as compared with that of butylated hydroxyanisole (BHA). Absorption maxima from UV spectra showed that flavonoids, and not phenolic acids, were the main phenolic compounds in separated fractions.

scholarly journals Antioxidant activity of rye caryopses and embryos extracts

2011 ◽  
Vol 20 (No. 6) ◽  
pp. 209-214 ◽  
Author(s):  
M. Karamać ◽  
R. Amarowicz ◽  
S. Weidner ◽  
S. Abe ◽  
F. Shahidi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Beta Carotene ◽  
Uv Spectra ◽  
Model System ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Scavenging Effect

Phenolic compounds were extracted with 80% methanol from caryopses and embryos of rye (cv. Dańkowskie Złote and Amilo). In all extracts, reducing power, scavenging effect on DPPH radical, and antioxidant activity in a β-carotene-linoleate model system were examined. The highest content of total phenolic compounds was noted in the extract from caryopses of Amilo (7.93 mg/g of extract). UV spectra of all extracts were characterised by maxima originated from phenolic acids (320, 326 and 328 nm), and by maxima at shorter wavelengths (272 and 274 nm) attributed to other phenolic compounds. All extracts showed a good antioxidant activity in a β-carotene-linoleate model system. This activity was similar to that reported before in leguminous seeds extracts. The antioxidant activities of the extracts from the caryopses of Dańkowskie Złote and the embryos of both cultivars were very similar, especially during the second part of the incubation period. The extract of Amilo embryos showed a slightly weaker antioxidative effect. The weak antiradical effects in the experiments with DPPH radical and a weak reducing power were characteristic for all the extracts investigated.  

Total phenolics and antioxidant activity of a functional gel based on AÇAÍ (euterpe oleracea martius) pulp

2015 ◽  
Vol 3 (3) ◽  
pp. 252-259 ◽  
Author(s):  
Lucia Maria Jaeger Carvalho
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Physical Activities ◽  
Total Phenolic ◽  
Euterpe Oleracea ◽  
Total Phenolic Compounds ◽  
Beneficial Effects ◽  
Dpph Method

The beneficial effects attributed to açaí and its derivatives are associated with its significant content of phenolic compounds, which has stimulated multiple/many researchers to identify these compounds and demonstrate their pharmacological properties, which include anti-inflammatory and antioxidant activities. The aim of this study was to determine the concentration of total phenolic compounds and the antioxidant activity of lyophilized pulps and functional gels of açaí. The antioxidant activity was the determining variable for the choice of the best formulation for the supplement. Pulp L exhibited higher antioxidant activity than the others according to the DPPH method, as well as higher content of total phenolic compounds. For the antioxidant activity, gels 3 and 4 did not differ significantly for the DPPH method, whereas in the ORAC method, gels 1 and 3 showed greater Trolox equivalents. It was concluded that gels 3 and 4 which had higher concentrations of lyophilized açaí pulp, showed higher concentrations of total phenolic compounds and consequently higher antioxidant capacity as demonstrated by the two methods applied and by the results obtained in the statistical analysis. Functional açaí gel may be used by adults who are involved in physical activities to reduce oxidative stress.

scholarly journals Antioxidant Compounds, Mineral Content and Antioxidant Activity of Several Tomato Cultivars Grown in Southwestern Romania

2013 ◽  
Vol 41 (1) ◽  
pp. 136 ◽  
Author(s):  
Violeta NOUR ◽  
Ion TRANDAFIR ◽  
Mira E. IONICA
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Trace Elements ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Fruit Shape ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Tomato Cultivars

Ten indeterminate tomato cultivars differing in fruit shape and typology, grown simultaneously in a greenhouse from Oltenia (Southwestern Romania) and harvested at red ripe stage, were investigated for the contents in ascorbic acid, lycopene, β-carotene, total phenolics, minerals (K, Na, Ca and Mg) and trace elements (Fe, Cu, Mn, Cr, Zn and B). In addition, their antioxidant activities were determined. Significant differences were found among tomato cultivars in all studied antioxidant compounds, as well as in the antioxidant activity. Ascorbic acid ranged from 91.9 to 329.7 mg kg-1 fw, lycopene ranged from 19.7 to 49.0 mg kg-1 fw, while total phenolic compounds varied between 300.2 and 557.8 mg kg-1 fw. Antioxidant activity ranged from 0.81 mmol Trolox kg-1 fw to 1.74 mmol Trolox kg-1 fw and it was significantly correlated to total phenolics content (r = 0.91; p< 0.05). The cocktail type cultivar ‘Tiger’ and the cherry type cultivar ‘Belle’ proved to be the most powerful in antioxidant activity and phenolic compounds while the rectangular plum shaped cultivar ‘Porto’ recorded the highest average lycopene content. A valuable cultivar proved to be the brownish red ‘Sacher’ which registered among the highest contents of phenolics, lycopene and ascorbic acid. Mineral and trace elements contents were also significantly affected by cultivars. Values recorded for K, Ca and Mg ranged from 2139.6 to 3056.9 mg kg-1, 137.7 to 325.8 mg kg-1 and 27.3 to 168.7 mg kg-1 respectively.

scholarly journals Antioxidant activity of aqueous and alcohol extracts of Salvia leriifolia L. and Linum usitalissmum L. subjected to a pulsed electric field

2020 ◽  
pp. 186-195
Author(s):  
Shohreh Arab Shirazi ◽  
Ahmad Pedram Nia ◽  
Mohammad Reza Saeidi Asl ◽  
Fariba Naghipour ◽  
Hamid Tavakolipour
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Electric Field ◽  
Antioxidant Activities ◽  
Pulse Electric Field ◽  
Antioxidant Properties ◽  
Pulsed Electric Field ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Positive Effects

Introduction. More attention has been paid in recent decades to extracts and essential oils from various plants as natural antioxidant sources due to their positive effects on food oxidation reactions. Our study aimed to compare the antioxidant activity of aqueous and alcoholic extracts from Salvia leriifolia L. and Linum usitalissmum L.The extracts were subjected to a pulsed electric field with intensities of zero (without pretreatment), 3 and 6 kV·cm–1, and a constant pulse number of 30. For this purpose, parameters such as total phenolic compounds and antioxidant activity were investigated by DPPH and TEAC methods. Results and discussion. Our results showed that a higher intensity of a pulsed electric field pretreatment and the use of an alcoholic solvent significantly raised total phenolic compounds in the extracts and their antioxidant activity at a 95% confidence level. We found significant effects of the plant source (Linum usitalissmum and Salvia leriifolia), pretreatment (pulse electric field at intensities of 0.3 and 6 kV·cm–1), and a solvent (aqueous and alcohol) on the extracts’ antioxidant activity (P < 0.05). In addition, there was a significant correlation between the results of the DPPH and the TEAC antioxidant activities (P < 0.01 and r = 0.932). Conclusion. The total antioxidant activity (based on both TEAC and DPPH methods) and total phenolic compounds extracted from Salvia leriifolia were higher than those from Linum usitalissmum (P < 0.05). Based on the results, the extract obtained from Salvia leriifolia with an alcoholic solvent and a pulsed electric field pretreatment (at 6 kV·cm–1 and 30 pulses) was selected as possessing desired antioxidant properties.

scholarly journals Kandungan Pigmen, Total Fenolik Dan Aktivitas Antioksidan Sargassum sp.

2018 ◽  
Vol 21 (2) ◽  
pp. 137
Author(s):  
Sri Sedjati ◽  
Endang Supriyantini ◽  
Ali Ridlo ◽  
Nirwani Soenardjo ◽  
Victorina Yulina Santi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Chlorophyll A ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Chlorophyll B ◽  
Total Phenolic Compounds ◽  
Total Phenolic Compound ◽  
Ic50 Value

Pigmen content, total phenolic compound and antioxidant activity Sargassum sp.   Sargassum sp. contains secondary metabolites which potentially act as natural source of antioxidant. The purpose of this study was to determine bioactive contents (pigments, total phenolic compounds), and antioxidant activities of Sargassum sp. The method of this research is descriptive-explorative.  Sample was extracted with methanol, while pigments extraction with aseton 80%.  Chlorophyll a, b and carotenoids were carried out with spectrophotometer method. The total phenolic compounds were analyzed by spectrophotometer method using Follin-Ciocalteu reagent, and antioxidant activities were measured using DPPH (1,1-diphenyl-2- picrylhidrazyl) method. The result of this research showed that methanol extract of Sargassum sp. had the presence of chlorophyll a 2.84 mg/g, chlorophyll b 1.15 mg/g, and carotenoids 2.63 μmol/g sample. The total phenolic compounds of methanol extract were 57.97 mg GAE/g sample. Methanol extract of Sargassum sp. was showing a strong antioxidant activity that could be seen in IC50 value (69,27 ppm) in less than 100 ppm concentration. Sargassum sp. mengandung beberapa metabolit sekunder yang berpotensi sebagai sumber antioksidan alami. Tujuan dari penelitian ini adalah mengkaji komponen bioaktif (pigmen, total fenolik) dan aktivitas antioksidan dari rumput laut Sargassum sp. Metoda penelitian ini adalah diskriptif eksploratif.  Sampel Sargassum sp. basah diekstraksi dengan cara maserasi dengan pelarut  metanol, sedangkan ekstraksi pigmen menggunakan aseton 80%. Penentuan kadar pigmen (klorofil a, b, karotenoid ), total fenolik dan aktivitas antioksidan berdasarkan metode spektrofotometri. Total fenolik diukur berdasarkan uji Follin-Ciocalteu, dan   aktivitas antioksidan  berdasarkan uji DPPH (1,1-diphenyl-2- picrylhidrazyl). Hasil penelitian menunjukan bahwa ekstrak metanol Sargassum sp.  mengandung pigmen klorofil a sebesar 2,84 mg/g, klorofil b sebesar 1,15 mg/g, dan karotenoid sebesar 2,63 μmol/g. Kadar total fenolik ekstrak metanol adalah 57,97 mgGAE/g, sementara aktivitas antioksidannya  (IC50) sebesar 69,27 ppm.  Berdasarkan nilai IC50nya, ekstrak metanol Sargassum sp berpotensi sebagai antioksidan kuat (kurang dari 100 ppm).

scholarly journals Extraction of Phenolic Compounds with Antioxidant Activity from Strawberries: Modelling with Artificial Neural Networks (ANNs)

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2228
Author(s):  
Iman Golpour ◽  
Ana Cristina Ferrão ◽  
Fernando Gonçalves ◽  
Paula M. R. Correia ◽  
Ana M. Blanco-Marigorta ◽  
...  
Keyword(s):  
Neural Networks ◽  
Antioxidant Activity ◽  
Artificial Neural Networks ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Feed Forward ◽  
Backpropagation Network ◽  
Artificial Neural

This research study focuses on the evaluation of the total phenolic compounds (TPC) and antioxidant activity (AOA) of strawberries according to different experimental extraction conditions by applying the Artificial Neural Networks (ANNs) technique. The experimental data were applied to train ANNs using feed- and cascade-forward backpropagation models with Levenberg-Marquardt (LM) and Bayesian Regulation (BR) algorithms. Three independent variables (solvent concentration, volume/mass ratio and extraction time) were used as ANN inputs, whereas the three variables of total phenolic compounds, DPPH and ABTS antioxidant activities were considered as ANN outputs. The results demonstrate that the best cascade- and feed-forward backpropagation topologies of ANNs for the prediction of total phenolic compounds and DPPH and ABTS antioxidant activity factors were the 3-9-1, 3-4-4-1 and 3-13-10-1 structures, with the training algorithms of trainlm, trainbr, trainlm and threshold functions of tansig-purelin, tansig-tansig-tansig and purelin-tansig-tansig, respectively. The best R2 values for the predication of total phenolic compounds and DPPH and ABTS antioxidant activity factors were 0.9806 (MSE = 0.0047), 0.9651 (MSE = 0.0035) and 0.9756 (MSE = 0.00286), respectively. According to the comparison of ANNs, the results showed that the cascade-forward backpropagation network showed better performance than the feed-forward backpropagation network for predicting the TPC, and the FFBP network, in predicting the DPPH and ABTS antioxidant activity factors, had more precision than the cascade-forward backpropagation network. The ANN technique is a potential method for estimating targeted total phenolic compounds and the antioxidant activity of strawberries.

scholarly journals Carotenoids, Flavonoids, Total Phenolic Compounds and Antioxidant Activity of Two Creeping Cotoneaster Species Fruits Extracts

2001 ◽  
Vol 71 (3) ◽  
pp. 136-142
Author(s):  
Dan Razvan Popoviciu ◽  
Ticuta Negreanu-Pirjol ◽  
Ludmila Motelica ◽  
Bogdan Stefan Negreanu Pirjol
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Ornamental Plants ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Methanol Extraction ◽  
Total Antioxidant ◽  
Trolox Equivalent

Cotoneaster horizontalis Decne. and Cotoneaster microphyllus Wall. ex Lindl. species are two creeping bushes, commonly used as ornamental plants in gardens and parks. The aim of this paper was to assess the concentrations of some classes of bioactive compounds classes, carotenoids, flavonoids and total phenolic compounds, in fresh fruits of these species. Carotenoids and flavonoids were determined through acetone and methanol extraction followed by spectrophotometry. For total phenolics, methanol extraction and a spectrophotometric Folin-Ciocalteu method was used. The total antioxidant capacity was quantified through photochemiluminescence method by comparison with the standard substance used for calibration, Trolox� as tocopherol analogue (ACL procedure) using Photochem apparatus, Analytik Jena AG, Germany. Average values found in Cotoneaster horizontalis and Cotoneaster microphyllus fruit tissue were 380.63 mg/kg, respectively 179.63 mg/kg, carotenoids; 8036.07 mg/kg, respectively 6888.06 mg/kg flavonoids; and 16342.06 mg/kg GAE, respectively 18631.35 mg/kg GAE total phenolic compounds. These values are comparable to those found in other wild and cultivated related Rosaceae, including domestic rowans. Cotoneaster microphyllus fruits emphasized an increased antioxidant activity (up to 39.69 μmol Trolox equivalent/g dry weight).

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

Vegetative propagation, chemical composition and antioxidant activity of yerba mate genotypes

2021 ◽  
pp. 1-10
Author(s):  
Leandro Marcolino Vieira ◽  
Renata de Almeida Maggioni ◽  
Jéssica de Cássia Tomasi ◽  
Erik Nunes Gomes ◽  
Ivar Wendling ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vegetative Propagation ◽  
Adventitious Rooting ◽  
Ilex Paraguariensis ◽  
Total Phenolic ◽  
Yerba Mate ◽  
Total Phenolic Compounds ◽  
Leaf Retention ◽  
Correlated Components

Abstract Ilex paraguariensis, commonly known as yerba mate, is a tree species native to South America. Its commercial value is due to the manufacturing of teas, with potential also in the pharmacological and cosmetic industries. Vegetative propagation of yerba mate is considered an innovation to the traditional production systems based on sexual propagation. The present study aimed to evaluate the rhizogenic potential and chemical attributes of mini-cuttings from 15 yerba mate genotypes, as well as to verify the correlation between phytochemical and rooting-related variables. Mini-cuttings were collected from a pre-existing mini-clonal hedge and the experimental design was completely randomized, with 15 treatments (genotypes), four replications and 10 mini-cuttings per plot. After 120 days, mini-cuttings were assessed regarding rooting, mortality, callogenesis and leaf retention percentages, percentage of mini-cuttings with both calluses and roots, number of roots and average root length. At the time of collection, subsamples from each plot were used for phytochemical analyses including total phenolic compounds, protein, caffeine and theobromine contents and antioxidant activity. Rooting percentages ranged from 5 to 72.5%, with significant variation among genotypes. Adventitious rooting and phytochemical profile of yerba mate mini-cuttings are genotype-dependent. Leaf retention is a relevant factor in the rooting of yerba mate mini-cuttings and the levels of total phenolic compounds, antioxidants and theobromine present in mini-cuttings are negatively correlated components to Ilex paraguariensis adventitious rooting.

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