The effects of in vitro exposure to progesterone and estradiol-17&beta; on the activity of canine neutrophils

To date, only limited information about the influence of ovarian hormones on canine immune system cells has been available. The study investigated the in vitro influence of progesterone and estradiol-17β on the activity of canine neutrophils. Treatment of cells by both hormones led to a significant decrease in phagocytosis-induced oxidative burst as detected using luminometry after stimulation with opsonised zymosan. The increase in oxidative burst, not connected with phagocytosis, was recorded after stimulation with a soluble stimulator. Using flow cytometry, the tendency of both hormones to decrease the production of reactive oxygen species associated with phagocytosis of Escherichia coli was also evident, although not significant. Suppression of canine neutrophil activity is not connected with pathogen recognition capabilities, since the expression of Toll-like receptor 4 was unaffected. This study reveals that both hormones have a suppressive effect on the activity of canine neutrophils and thus might contribute to the aetiology of pyometra.  

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Activation of Toll-Like Receptor 4 (TLR4) by In Vivo and In Vitro Exposure of Rat Epididymis to Lipopolysaccharide from Escherichia Coli1

Biology of Reproduction ◽

10.1095/biolreprod.108.069930 ◽

2008 ◽

Vol 79 (6) ◽

pp. 1135-1147 ◽

Cited By ~ 53

Author(s):

Alexandre Rodrigues ◽

Daniel B.C. Queiróz ◽

Luciana Honda ◽

Erick José R. Silva ◽

Susan H. Hall ◽

...

Keyword(s):

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

In Vitro Exposure ◽

Rat Epididymis

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Lipopolysaccharides Belonging to Different Salmonella Serovars Are Differentially Capable of Activating Toll-Like Receptor 4

Infection and Immunity ◽

10.1128/iai.02297-14 ◽

2014 ◽

Vol 82 (11) ◽

pp. 4553-4562 ◽

Cited By ~ 8

Author(s):

Daniela Chessa ◽

Luisella Spiga ◽

Nicola De Riu ◽

Paola Delaconi ◽

Vittorio Mazzarello ◽

...

Keyword(s):

Immune Response ◽

Salmonella Enterica ◽

Systemic Infection ◽

Embryonic Cell ◽

Limited Information ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Content Type ◽

Serovar Typhimurium

ABSTRACTSalmonella entericasubsp.entericaserovar (serotype) Abortusovis is a member of theEnterobacteriaceae. This serotype is naturally restricted to ovine species and does not infect humans. Limited information is available about the immune response of sheep toS. Abortusovis.S. Abortusovis, likeSalmonella entericasubsp.entericaserovar Typhi, causes a systemic infection in which, under natural conditions, animals are not able to raise a rapid immune response. Failure to induce the appropriate response allows pathogens to reach the placenta and results in an abortion. Lipopolysaccharides (LPSs) are pathogen-associated molecular patterns (PAMPs) that are specific to bacteria and are not synthesized by the host. Toll-like receptors (TLRs) are a family of receptors that specifically recognize PAMPs. As a first step, we were able to identify the presence of Toll-like receptor 4 (TLR4) on the ovine placenta by using an immunohistochemistry technique. To our knowledge, this is the first work describing the interaction betweenS. Abortusovis LPS and TLR4. Experiments using an embryonic cell line (HEK293) transfected with human and ovine TLR4s showed a reduction of interleukin 8 (IL-8) production byS. Abortusovis andSalmonella entericasubsp.entericaserovar Paratyphi upon LPS stimulation compared toSalmonella entericasubsp.entericaserovar Typhimurium. Identical results were observed using heat-killed bacteria instead of LPS. Based on data obtained with TLR4in vitrostimulation, we demonstrated that the serotypeS. Abortusovis is able to successfully evade the immune system whereasS. Typhimurium and other serovars fail to do so.

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Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients

Biomedicines ◽

10.3390/biomedicines9060599 ◽

2021 ◽

Vol 9 (6) ◽

pp. 599

Author(s):

Víctor Farré-Alins ◽

Alejandra Palomino-Antolín ◽

Paloma Narros-Fernández ◽

Ana Belen Lopez-Rodriguez ◽

Céline Decouty-Perez ◽

...

Keyword(s):

Injury Severity ◽

White Blood Cells ◽

Serum Amyloid ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Important Link ◽

Tlr4 Mrna ◽

Serum Amyloid A1

Traumatic brain injury (TBI) is one of the leading causes of mortality and disability worldwide without any validated biomarker or set of biomarkers to help the diagnosis and evaluation of the evolution/prognosis of TBI patients. To achieve this aim, a deeper knowledge of the biochemical and pathophysiological processes triggered after the trauma is essential. Here, we identified the serum amyloid A1 protein-Toll-like receptor 4 (SAA1-TLR4) axis as an important link between inflammation and the outcome of TBI patients. Using serum and mRNA from white blood cells (WBC) of TBI patients, we found a positive correlation between serum SAA1 levels and injury severity, as well as with the 6-month outcome of TBI patients. SAA1 levels also correlate with the presence of TLR4 mRNA in WBC. In vitro, we found that SAA1 contributes to inflammation via TLR4 activation that releases inflammatory cytokines, which in turn increases SAA1 levels, establishing a positive proinflammatory loop. In vivo, post-TBI treatment with the TLR4-antagonist TAK242 reduces SAA1 levels, improves neurobehavioral outcome, and prevents blood–brain barrier disruption. Our data support further evaluation of (i) post-TBI treatment in the presence of TLR4 inhibition for limiting TBI-induced damage and (ii) SAA1-TLR4 as a biomarker of injury progression in TBI patients.

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Detrimental Role of Heat Shock Protein 60&70 and Toll-like Receptor 4 in Skeletal Muscle Ischaemia In Vitro

Journal of Vascular Surgery ◽

10.1016/j.jvs.2013.02.134 ◽

2013 ◽

Vol 57 (5) ◽

pp. 77S

Author(s):

Ali Navi ◽

Rebekah Yu ◽

Xu Shi-Wen ◽

Sidney Shaw ◽

George Hamilton ◽

...

Keyword(s):

Skeletal Muscle ◽

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 60 ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Muscle Ischaemia

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Soyasaponin Bb inhibits the recruitment of toll-like receptor 4 (TLR4) into lipid rafts and its signaling pathway by suppressing the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-dependent generation of reactive oxygen species

Molecular Nutrition & Food Research ◽

10.1002/mnfr.201600015 ◽

2016 ◽

Vol 60 (7) ◽

pp. 1532-1543 ◽

Cited By ~ 12

Author(s):

Yajie Zhang ◽

Fengping Chen ◽

Jiading Chen ◽

Suqun Huang ◽

Junbin Chen ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Nadph Oxidase ◽

Signaling Pathway ◽

Lipid Rafts ◽

Nicotinamide Adenine Dinucleotide ◽

Nicotinamide Adenine Dinucleotide Phosphate ◽

Nicotinamide Adenine ◽

Oxygen Species ◽

Toll Like Receptor ◽

Toll Like Receptor 4

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Reactive Oxygen Species-Induced Expression of B cell Activating Factor (BAFF) Is Independent of Toll-like Receptor 4 and Myeloid Differentiation Primary Response Gene 88

Biomolecules & Therapeutics ◽

10.4062/biomolther.2009.17.2.144 ◽

2009 ◽

Vol 17 (2) ◽

pp. 144-150 ◽

Cited By ~ 7

Author(s):

Hyun-Sun Kim ◽

Eun-Yi Moon

Keyword(s):

Reactive Oxygen Species ◽

B Cell ◽

Primary Response ◽

Myeloid Differentiation ◽

Oxygen Species ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Response Gene ◽

B Cell Activating Factor ◽

Primary Response Gene

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Ginsenoside Rb1 exerts anti-inflammatory effects in vitro and in vivo by modulating toll-like receptor 4 dimerization and NF-kB/MAPKs signaling pathways

Phytomedicine ◽

10.1016/j.phymed.2020.153197 ◽

2020 ◽

Vol 69 ◽

pp. 153197 ◽

Cited By ~ 5

Author(s):

Hongwei Gao ◽

Naixin Kang ◽

Chao Hu ◽

Ziyu Zhang ◽

Qiongming Xu ◽

...

Keyword(s):

Signaling Pathways ◽

Ginsenoside Rb1 ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Anti Inflammatory

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Micrococcus luteus Teichuronic Acids Activate Human and Murine Monocytic Cells in a CD14- and Toll-Like Receptor 4-Dependent Manner

Infection and Immunity ◽

10.1128/iai.69.4.2025-2030.2001 ◽

2001 ◽

Vol 69 (4) ◽

pp. 2025-2030 ◽

Cited By ~ 32

Author(s):

Shuhua Yang ◽

Shunji Sugawara ◽

Toshihiko Monodane ◽

Masahiro Nishijima ◽

Yoshiyuki Adachi ◽

...

Keyword(s):

Lipid A ◽

Micrococcus Luteus ◽

Dependent Manner ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Necrosis Factor Alpha ◽

Monocytic Cells ◽

Tnf Α

ABSTRACT Teichuronic acid (TUA), a component of the cell walls of the gram-positive organism Micrococcus luteus (formerlyMicrococcus lysodeikticus), induced inflammatory cytokines in C3H/HeN mice but not in lipopolysaccharide (LPS)-resistant C3H/HeJ mice that have a defect in the Toll-like receptor 4 (TLR4) gene, both in vivo and in vitro, similarly to LPS (T. Monodane, Y. Kawabata, S. Yang, S. Hase, and H. Takada, J. Med. Microbiol. 50:4–12, 2001). In this study, we found that purified TUA (p-TUA) induced tumor necrosis factor alpha (TNF-α) in murine monocytic J774.1 cells but not in mutant LR-9 cells expressing membrane CD14 at a lower level than the parent J774.1 cells. The TNF-α-inducing activity of p-TUA in J774.1 cells was completely inhibited by anti-mouse CD14 monoclonal antibody (MAb). p-TUA also induced interleukin-8 (IL-8) in human monocytic THP-1 cells differentiated to macrophage-like cells expressing CD14. Anti-human CD14 MAb, anti-human TLR4 MAb, and synthetic lipid A precursor IVA, an LPS antagonist, almost completely inhibited the IL-8-inducing ability of p-TUA, as well as LPS, in the differentiated THP-1 cells. Reduced p-TUA did not exhibit any activities in J774.1 or THP-1 cells. These findings strongly suggested that M. luteus TUA activates murine and human monocytic cells in a CD14- and TLR4-dependent manner, similar to LPS.

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