scholarly journals Immunohistochemical characterisation of a canine case of granular cell type trichoblastoma: a case report

2014 ◽  
Vol 59 (No. 10) ◽  
pp. 511-514
Author(s):  
JS Yoon ◽  
J. Park
Keyword(s):  
Periodic Acid ◽  
Granular Cell ◽  
Stem Cell Marker ◽  
Cell Type ◽  
Intact Male ◽  
Periodic Acid Schiff ◽  
Cytoplasmic Staining ◽  
Fibrous Stroma ◽  
Clear Cells ◽  
Cytoplasmic Vacuoles

Herein we describe a case of granular cell type trichoblastoma in a dog. A nine-year-old, intact male Scottish terrier presented with a solitary, exophytic and non-ulcerated nodule on the shoulder. Histopathologically, the nodule showed a ribbon-like structure, which was composed of branching cords of epithelial cells surrounded by fibrous stroma. In addition, islands of large clear cells were also noted. The clear cells showed eccentric nuclei and abundant cytoplasmic vacuoles, which were strongly positive for periodic acid-Schiff staining. Cytoplasmic immunostaining for cytokeratin (CK) 14 and nuclear immunostaining for p63 were observed in the neoplastic cells. In contrast, CK18 staining was scant. Furthermore, the hair bulge stem cell marker CK15 showed strong cytoplasmic staining. Based on these histopathological and immunohistochemical findings, the tumour was diagnosed as a granular cell type trichoblastoma, which is rarely reported in dogs.  

scholarly journals Atypical meningeal granular cell tumor in a dog

2011 ◽  
Vol 24 (1) ◽  
pp. 192-197 ◽  
Author(s):  
Sasmita Mishra ◽  
Marc Kent ◽  
Allison Haley ◽  
Simon Platt ◽  
Kaori Sakamoto
Keyword(s):  
Cerebral Hemisphere ◽  
Granular Cell Tumor ◽  
Periodic Acid ◽  
Granular Cell ◽  
Cell Tumor ◽  
Ultrastructural Changes ◽  
Periodic Acid Schiff ◽  
Falx Cerebri ◽  
Cytoplasmic Staining ◽  
Left Cerebral Hemisphere

A 10-year-old, female spayed Chihuahua dog was presented to the Veterinary Teaching Hospital at the University of Georgia for evaluation of seizures, abnormal mentation, and cervical pain of 2 months duration. On magnetic resonance imaging, there was generalized thickening of the meninges overlying the left cerebral hemisphere and along the falx cerebri. Despite symptomatic treatment, the dog remained neurologically affected. Consequently, the owners elected euthanasia. On gross examination, the meninges covering the left cerebral hemisphere were severely thickened and firmly adhered to the calvaria. On transverse section, the white matter of the left cerebral hemisphere was swollen, enlarged, and extended across the midline with resultant compression of the right cerebral hemisphere. Cytologic evaluation of an impression smear of the thickened meninges showed numerous large, spindloid to polygonal cells with abundant, amphophilic, vacuolated cytoplasm, present either in clusters or in individual cells. Histopathologic evaluation of the meninges revealed a poorly circumscribed and infiltrative, moderately cellular neoplasm, composed of vacuolated, spindloid to polygonal cells with marked anisocytosis and anisokaryosis, arranged in sheets, and occasionally separated by thick bands of connective tissue. Immunohistochemistry for vimentin revealed diffuse cytoplasmic staining of the neoplastic cells. Although the periodic acid–Schiff reaction was negative, ultrastructural findings showed numerous vesicles that were empty or that contained membranous or electron-dense material. Based on gross, microscopic, immunohistochemical, and ultrastructural changes, the meningeal neoplasm was diagnosed as an atypical granular cell tumor.

scholarly journals β-Catenin and Periodic Acid–Schiff Distinguish Granular Cell Nevus From Deep Penetrating Nevus

2021 ◽  
Vol 145 (12) ◽  
pp. 1475-1476
Author(s):  
Maya Eiger-Moscovich ◽  
Ralph C. Eagle ◽  
Tatyana Milman
Keyword(s):  
Periodic Acid ◽  
Granular Cell ◽  
Periodic Acid Schiff

scholarly journals A Case of Canine Apocrine Sweat Gland Adenoma, Clear Cell Variant

2005 ◽  
Vol 42 (2) ◽  
pp. 215-218 ◽  
Author(s):  
K. Nibe ◽  
K. Uchida ◽  
T. Itoh ◽  
S. Tateyama
Keyword(s):  
Sweat Gland ◽  
Clear Cell ◽  
Smooth Muscle Actin ◽  
Periodic Acid ◽  
Alpha Smooth Muscle Actin ◽  
Periodic Acid Schiff ◽  
Clear Cells ◽  
Apocrine Sweat Gland ◽  
Cell Variant ◽  
Clear Cell Variant

A cutaneous mass at the base of the retroauricular region of a 4-year-old, female Golden Retriever was examined pathologically. Histologically, the mass formed multiple nodules consisting of a proliferation of large clear cells with abundant cytoplasm. Mitotic figures among the neoplastic cells were very sparse. The large clear cells were intensely positive for cytokeratins (AE1/AE4, cytokeratin 8 and 18) and moderately positive for lysozyme and contained periodic acid-Schiff-positive granules in the cytoplasm. In addition, small flat cells lined the islands of neoplastic large clear cells, and these were strongly positive for alpha-smooth muscle actin and vimentin, and some were positive for cytokeratin (AE1/AE4), suggesting they were myoepithelial cells. No local recurrence or metastasis has been recognized during the 18 months since surgical excision. On the basis of these findings, the present tumor was diagnosed as apocrine sweat gland adenoma, clear cell variant. There have been few previous reports of canine apocrine adenomas showing a clear cell morphology.

Histologic and Immunohistochemical Studies of Granular Cell Tumors in Seven Dogs, Three Cats, One Horse, and One Bird

1993 ◽  
Vol 30 (2) ◽  
pp. 176-185 ◽  
Author(s):  
A. K. Patnaik
Keyword(s):  
Incidental Finding ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Granular Cell ◽  
The Other ◽  
Intracranial Meningioma ◽  
Surgical Biopsy ◽  
Periodic Acid Schiff ◽  
Rhabdoid Cells ◽  
Eosinophilic Cytoplasm

In a retrospective study, granular cell tumors in six dogs (Nos. 1–6), three cats (Nos. 1–3), one horse (No. 1), and one cockatiel ( Nymphicus hollandicus) (No. 1) and a meningioma with a granular cell component in one dog (No. 7) were examined histologically and immunohistochemically. These tumors were identified by histologic examination of surgical biopsy specimens, except in the horse, in which the tumor was an incidental finding at necropsy. These diagnoses were initially made by more than one pathologist. Five of the six granular cell tumors in the dogs were in the oral cavity; one of these was in the maxillary gingiva of a 6-month-old puppy. The tumors in the cats were located in the tongue, vulva, and digit. The tumor in the horse was in the lung, and the tumor in the cockatiel was in the periocular tissue. Histologically, all granular cell tumors were characterized by oval to polygonal cells of various sizes. The cells had abundant, pale, eosinophilic cytoplasm with distinct intracytoplasmic granules, distinct cell margins, and mostly central nuclei. In the dogs, the gingival tumor had a large amount of collagen tissue, the tumor in the tongue had dilated blood vessels, and the maxillary tumor in the puppy was more cellular than the other tumors. The tumors in the cats were more anaplastic than the other tumors; one, located in the digit, was considered malignant. The granules in all of the tumors stained with periodic acid-Schiff and were diastase resistant. On staining with Luxol fast blue, the granules of all tumors stained different shades of pink, with the exception of the tumor in the tongue of a cat, which stained bluish green. Immunocytochemically, all tumors except the tumor in the cockatiel reacted against antibodies to vimentin. The granular cell tumor in the lung of the horse and the intracranial meningioma in a dog reacted to the antibody S-100 protein; the tumor in the horse reacted to neuron-specific enolase; tumors in two dogs (gingiva and skin) reacted to L-antitrypsin, and the maxillary tumor also reacted to lysozyme; the malignant tumor in the digit of a cat and the periocular tumor in the cockatiel reacted to muscle common actin and actin; the tumor in the cockatiel also reacted to desmin. Results of these immunocytochemical studies suggest that granular cell tumors, like tumors composed of rhabdoid cells, clear cells, and oncocytes, can have similar morphologic features but be of different cellular origins.

Canine Peripheral Nerve Sheath Tumor with Eosinophilic Cytoplasmic Globules

1998 ◽  
Vol 35 (3) ◽  
pp. 223-226 ◽  
Author(s):  
M. Kuwamura ◽  
J. Yamate ◽  
T. Kotani ◽  
T. Takeuchi ◽  
S. Sakuma
Keyword(s):  
Peripheral Nerve ◽  
Tumor Cells ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Granular Cell ◽  
Nerve Sheath Tumor ◽  
Periodic Acid Schiff ◽  
Peripheral Nerve Sheath Tumor ◽  
Nerve Sheath ◽  
Spindle Cell Proliferation

A 13-year-old male Shetland Sheepdog had a subcutaneous tumor in the left brachium. The tumor was removed and recurred several times at 5, 13, 16, 22, and 31 months after the initial presentation. Histologically, the removed nodules from the fourth resection were composed of neoplastic proliferation of round to fusiform cells, which possessed eosinophilic globules in their cytoplasm. The globules were periodic acid–Schiff positive and diastase resistant. Positive reactions for acid phosphatase were observed in the cytoplasm of the tumor cells. Ultrastructurally, these globules consisted of membrane-bound, dense structures containing dense granules, lucent vacuoles, and homogeneous materials. The recurrent tumors removed at the fifth resection consisted of spindle cell proliferation arranged in interlacing fascicles with wavy nuclei and containing a small number of cells with cytoplasmic globules. The tumor cells were immunoreactive to vimentin, S-100 protein, myelin basic protein, and neuron-specific enolase. The tumor was diagnosed as a peripheral nerve sheath tumor with eosinophilic cytoplasmic globules. These findings are unique for the histogenesis of granular cell tumors.

scholarly journals Recurrent pyogenic granuloma: an update

2015 ◽  
Vol 1 (1) ◽  
pp. 22 ◽  
Author(s):  
Rami A. Al-shiaty ◽  
Bacem A. E. Ottoman
Keyword(s):  
Alcian Blue ◽  
Periodic Acid ◽  
Pyogenic Granuloma ◽  
T Test ◽  
The Other ◽  
Dimensional Structure ◽  
Periodic Acid Schiff ◽  
Fibrous Stroma ◽  
Significant Difference ◽  
Initial Appearance

<p class="abstract"><strong><span lang="EN-US">Background: </span></strong>Given the fierce controversy about the nature of pyogenic granulomas, starting with its unfitting name and ending up with its ideal treatment modality, this paper tries to numerically identify some predisposing factors of recurrence.</p><p class="abstract"><strong><span lang="EN-US">Methods: </span></strong>The literature was initially reviewed and a total of twenty recurrent cases of pyogenic granuloma were contrasted, on one hand, to their initial appearance. On the other hand, all are contrasted to a similar number of normal mucosa using three histochemical stains: Alcian blue, periodic acid-Schiff and Masson’s trichrome.</p><p class="abstract"><strong><span lang="EN-US">Results: </span></strong>For all recurrent lesions, all specimens showed myxoid structure histologically even if their initial appearance had possessed a sparse myxoid structure. The age of recurrence has been correlated to the histochemical findings. For the Alcian Blue stain (AB), the value of t-test was 3.808840. The pertaining value of P was 0.000593. The result was significant at P ≤0.05. For the PAS stain, the value of t-test was 3.640327. The value of P was 0.000871. The result was significant at P ≤0.05. In Masson’s trichrome staining, the value of t-test was 3.100816. The value of P was 0.002942. The result was significant at P ≤0.05. Accordingly, all stains showed significant difference in fibrous content in the initial and recurrent lesions. Conversely, the count of both endothelial vessels and inflammatory infiltrates in the recurrent lesions were significantly lower than the primary precursors.</p><p class="abstract"><strong><span lang="EN-US">Conclusions: </span></strong>Given that collagen fibers are continually degraded and resynthesized while proteolytic degradation occur outside the cells through the activity of enzymes called  matrix metalloproteinases (MMPs), it is suggested that MMPs -positively expressed by PAS reactions- account for the spacing of the fibrous stroma, allowing for reshaping the three dimensional structure of the connective tissue. Myxoid structures are certainly promoting recurrence either via excessive secretion of hyaluronic acids or unknown mechanisms. The undisputed fact is the presence of myxoid structures in all our reported recurrent cases. Both inflammatory cascade and endothelial proliferation have no vital role in the recurrence according to our morphometric results. Finally, PAS stain should give more details in examining PGs than the other recruited counterparts.</p><p class="abstract"><strong><span lang="EN-US">Keywords: </span></strong><span lang="EN-US">Recurrent pyogenic granuloma, PAS stain, Myxoid structures, Etiopathogensis</span></p><p class="keywords"> </p>

scholarly journals Hepatocellular Carcinoma with Metastasis to the Spleen in a Holstein Cow

2005 ◽  
Vol 42 (2) ◽  
pp. 230-232 ◽  
Author(s):  
W. I. Jeong ◽  
S. H. Do ◽  
M. H. Sohn ◽  
H. S. Yun ◽  
O. D. Kwon ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Cells ◽  
Periodic Acid ◽  
Splenic Metastasis ◽  
Periodic Acid Schiff ◽  
Fibrous Stroma ◽  
Holstein Cow ◽  
Stromal Response ◽  
Immunohistochemical Methods ◽  
Immunohistochemical Studies

Hepatocellular carcinoma (HCC) with metastasis to the spleen in a Holstein cow was studied by histopathologic and immunohistochemical methods. The tumor was characterized by a pseudoglandular (acinar) pattern with an associated fibrous stroma. Individual cells often had a “hepatoid” appearance but were interspersed with scattered cells exhibiting a clear, periodic acid-Schiff (PAS)-positive cytoplasm and small eccentric nuclei. This pattern was present in nodules found in both liver and spleen. Moreover, hepatoid tumor cells were positive for alpha-fetoprotein. Immunohistochemical studies suggest that myofibroblasts were responsible for the production of fibrous septa surrounding the pseudoglandular structures of bovine HCC. In summary, our histologic and immunohistochemical findings support a diagnosis of primary HCC with splenic metastasis. Furthermore, the associated stromal response appears to be of a myofibroblast origin. The primary etiology of bovine HCC and the significance of the intralesional, PAS-positive clear cells remain undetermined.

Giant Granular Cell Tumor of the Suprasellar Area; Immunocytochemical and Electron Microscopic Studies

Neurosurgery ◽  
1984 ◽  
Vol 15 (2) ◽  
pp. 246-251 ◽  
Author(s):  
Boleslaw H. Liwnicz ◽  
Boleslaw H. Liwnicz ◽  
Regina G. Liwnicz ◽  
Stephen J. Huff ◽  
Bert H. McBride ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Granular Cell Tumor ◽  
Periodic Acid ◽  
Granular Cell ◽  
Cell Tumor ◽  
Electron Microscopic ◽  
Periodic Acid Schiff ◽  
Computed Tomographic ◽  
Suprasellar Region ◽  
Microscopic Studies

Abstract We describe a case of a granular cell tumor (GCT) of the suprasellar region with an 11-year history in a 26-year-old woman. The computed tomographic scan showed a midline, contrast-enhancing, noncalcified mass. The biopsy was diagnosed as GCT. The tumor was treated with radiation therapy. At necropsy, a large, homogeneous GCT surrounded by gliosis was found. The tumor cells were filled with granules positive for periodic acid-Schiff, diastase-resistant. The cells did not contain glial fibrillary acidic protein or S-100 protein. Electron microscopy showed tumor cells filled with innumerable lysosomal structures. No intermediate filament was found within the cytoplasm. The tumor cells were not surrounded by a basement membrane. Based on this study and on our review of the literature, the suggestion that GCT has a multicellular origin is upheld.

scholarly journals FERRITIN PARTICLES IN MACROPHAGES AND IN ASSOCIATED MAST CELLS

1972 ◽  
Vol 52 (3) ◽  
pp. 536-541 ◽  
Author(s):  
J. V. Simson ◽  
S. S. Spicer
Keyword(s):  
Lymph Node ◽  
Mast Cells ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Light Microscope Level ◽  
Reactive Material ◽  
Reactive Substance ◽  
Cytoplasmic Vacuoles ◽  
Intimate Association ◽  
The Matrix

In a variety of tissues (lymph node and glandular stroma), mast cells have been found in close and often intimate association with macrophages containing numerous ferritin-like particles in their cytoplasm and within cytoplasmic vacuoles (siderosomes). Phagocytic vacuoles in a given macrophage differed markedly. Some contained abundant Prussian blue-reactive material and others contained periodic acid-Schiff reactive substance at the light microscope level, and ultrastructurally some were filled with ferritin particles and others were not. Ferritin-like particles have also been observed occasionally in the mast cells associated with macrophages and even within the matrix of some of the granules in these mast cells.

Cytological Changes in the Pituitary Gland of the Adult Sockeye Salmon (Oncorhynchus nerka) after Gonadectomy

1969 ◽  
Vol 26 (5) ◽  
pp. 1147-1156 ◽  
Author(s):  
J. R. McBride ◽  
A. P. van Overbeeke
Keyword(s):  
Pituitary Gland ◽  
Sexual Maturity ◽  
Sockeye Salmon ◽  
Considerable Increase ◽  
Periodic Acid ◽  
Cell Type ◽  
Periodic Acid Schiff ◽  
Cytological Features ◽  
Cytological Changes ◽  
Immature Fish

Effects of gonadectomy on the cytological structure of the pituitary gland of sockeye salmon were investigated. Castration of fully grown, but sexually immature, fish appeared to prevent differentiation of any periodic acid–Schiff (PAS) positive cells. On the other hand, castration of mature sockeye induced degranulation of this cell type. These cells later disintegrated. Although the acidophil cells of the proximal pars distalis in the immature castrates showed an apparent increase in number, those in the gonadectomized ripe fish exhibited marked hyperplasia and hypertrophy. In view of the "rejuvenation," including the considerable increase in body weight that occurs after castration of sexually ripe sockeye, it is thought that the changes in the acidophils noted in this experiment may reflect an increased production of growth hormone. Where the gonadectomy was incomplete it was noted that very small remnants of gonad were able to induce, or maintain, all the external secondary characteristics of full sexual maturity. The pituitary of these fish exhibited cytological features intermediate between those of the controls and the complete castrates.

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