Cytological Changes in the Pituitary Gland of the Adult Sockeye Salmon (Oncorhynchus nerka) after Gonadectomy

1969 ◽  
Vol 26 (5) ◽  
pp. 1147-1156 ◽  
Author(s):  
J. R. McBride ◽  
A. P. van Overbeeke
Keyword(s):  
Pituitary Gland ◽  
Sexual Maturity ◽  
Sockeye Salmon ◽  
Considerable Increase ◽  
Periodic Acid ◽  
Cell Type ◽  
Periodic Acid Schiff ◽  
Cytological Features ◽  
Cytological Changes ◽  
Immature Fish

Effects of gonadectomy on the cytological structure of the pituitary gland of sockeye salmon were investigated. Castration of fully grown, but sexually immature, fish appeared to prevent differentiation of any periodic acid–Schiff (PAS) positive cells. On the other hand, castration of mature sockeye induced degranulation of this cell type. These cells later disintegrated. Although the acidophil cells of the proximal pars distalis in the immature castrates showed an apparent increase in number, those in the gonadectomized ripe fish exhibited marked hyperplasia and hypertrophy. In view of the "rejuvenation," including the considerable increase in body weight that occurs after castration of sexually ripe sockeye, it is thought that the changes in the acidophils noted in this experiment may reflect an increased production of growth hormone. Where the gonadectomy was incomplete it was noted that very small remnants of gonad were able to induce, or maintain, all the external secondary characteristics of full sexual maturity. The pituitary of these fish exhibited cytological features intermediate between those of the controls and the complete castrates.

The Identification of Thyrotrophin-secreting Cells in the Pituitary Gland of the Minnow (Phoxinus phoxinus)

1955 ◽  
Vol s3-96 (34) ◽  
pp. 193-201
Author(s):  
E. J. W. BARRINGTON ◽  
A. J. MATTY
Keyword(s):  
Pituitary Gland ◽  
Positive Response ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Phoxinus Phoxinus ◽  
Aldehyde Fuchsin ◽  
Median Zone ◽  
Cytological Characteristics

Evidence is given for the existence of two main types of cyanophil cell in the median zone of the glandular lobe (adenohypophysis) of the minnow, distinguishable by their distribution and by their cytological characteristics. Both types are positive to the periodic acid Schiff (PAS) technique, but one (type 2 of this account) also gives a positive response to the aldehyde-fuchsin (AF) technique of Gomori, as used by Halmi and by Purves and Griesbach in studies of the mammalian pituitary. In fish which have been immersed in thiouracil solution the type 2 cells show degranulation and vacuolation, and their characteristic positive AF response is very greatly weakened or lost. For these reasons the type 2 cells are believed to be responsible for the secretion of thyrotrophin, and appear to be very closely comparable with the thyrotroph cells of the pituitary of the rat.

Experimental bisection of Aquilegia floral buds cultured in vitro: II. Cytological changes following bisection

1972 ◽  
Vol 50 (7) ◽  
pp. 1611-1615 ◽  
Author(s):  
Lawrence C. W. Jensen
Keyword(s):  
Electron Microscope ◽  
Resting State ◽  
Electron Microscope Study ◽  
Developmental Stages ◽  
Periodic Acid ◽  
Progressive Increase ◽  
Progressive Decrease ◽  
Periodic Acid Schiff ◽  
Cytological Changes

A previous paper in this series has reported on the effect of bisecting Aquilegia floral apices of different developmental stages by maintaining a continuous photographic record of living buds (Jensen 1971). The current paper is a preliminary light and electron microscope study of the cytological changes which occur following bisection of very young buds (sepal to petal stages). Bisected buds show a progressive increase in size of grains which are positive to periodic acid – Schiff stain up to 2 days after incision, followed by a progressive decrease in size up to 7 days after incision. These grains are probably starch as they were absent in sections treated with α-amylase and malt diastase. It is suggested that after bisection the buds are in a resting state for 1 to 2 days during which time sucrose is absorbed from the medium and stored as starch. As regeneration of a new apex proceeds, starch is digested and used by the cells of the growing bud.

scholarly journals Immunohistochemical characterisation of a canine case of granular cell type trichoblastoma: a case report

2014 ◽  
Vol 59 (No. 10) ◽  
pp. 511-514
Author(s):  
JS Yoon ◽  
J. Park
Keyword(s):  
Periodic Acid ◽  
Granular Cell ◽  
Stem Cell Marker ◽  
Cell Type ◽  
Intact Male ◽  
Periodic Acid Schiff ◽  
Cytoplasmic Staining ◽  
Fibrous Stroma ◽  
Clear Cells ◽  
Cytoplasmic Vacuoles

Herein we describe a case of granular cell type trichoblastoma in a dog. A nine-year-old, intact male Scottish terrier presented with a solitary, exophytic and non-ulcerated nodule on the shoulder. Histopathologically, the nodule showed a ribbon-like structure, which was composed of branching cords of epithelial cells surrounded by fibrous stroma. In addition, islands of large clear cells were also noted. The clear cells showed eccentric nuclei and abundant cytoplasmic vacuoles, which were strongly positive for periodic acid-Schiff staining. Cytoplasmic immunostaining for cytokeratin (CK) 14 and nuclear immunostaining for p63 were observed in the neoplastic cells. In contrast, CK18 staining was scant. Furthermore, the hair bulge stem cell marker CK15 showed strong cytoplasmic staining. Based on these histopathological and immunohistochemical findings, the tumour was diagnosed as a granular cell type trichoblastoma, which is rarely reported in dogs.  

Is Rhabdospora thelohani (Laguesse) a sporozoan parasite or a tissue cell of lower vertebrates?

Parasitology ◽  
1966 ◽  
Vol 56 (4) ◽  
pp. 633-638 ◽  
Author(s):  
L. H. Bannister
Keyword(s):  
Periodic Acid ◽  
Taxonomic Status ◽  
Golgi Body ◽  
Tissue Cell ◽  
Apical Region ◽  
Cell Type ◽  
Periodic Acid Schiff ◽  
Basal Nucleus ◽  
Histological Techniques ◽  
Electron Microscopes

An examination with light and electron microscopes has been made of the cell type which is considered by some authors to be a sporozoan parasite, Rhabdospora thelohani, and by others to be a tissue cell of fishes and amphibians.The cell type was found in the minnow Phoxinus phoxinus in many tissue sites, including olfactory and pharygneal epithelia, kidney, eyes and brain. In some specimens it could not be found in any tissue.The cell has a thick fibrous wall, a basal nucleus, an apical mitochondrial zone, a Golgi body, and from 8 to 50 characteristic refractile rodlets.The rodlets give a negative reaction to the Feulgen test for nuclear material, but are positive to the periodic acid–Schiff stain, a test for polysaccharide. Glycogen does not appear to be present in the rodlets.Under certain circumstances the rods rupture the cell wall and may protrude in the apical region of the cell.The structures described are discussed with the evidence for and against a parasitic interpretation of the cell. It is concluded that Rhabdospora is a protozoan parasite, whose exact functional and taxonomic status is as yet unknown.I am grateful to Professor G. E. H. Foxon and to Professor P. C. C. Garnham for their encouragement, advice, and helpful criticism of the manuscript. I am further indebted to the National Spastics Society for the use of the electron microscope housed in Guy&s Hospital Medical School, and to Mr I. D. Bradbrook for his assistance with the histological techniques.

Inflamed Conjunctival Nevi: Histopathological Criteria

2014 ◽  
Vol 138 (9) ◽  
pp. 1242-1246 ◽  
Author(s):  
Eunice K. Choi ◽  
Patricia Chévez-Barrios
Keyword(s):  
Plasma Cells ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Melanocytic Lesion ◽  
Scoring Method ◽  
Diagnostic Features ◽  
Periodic Acid Schiff ◽  
Complete Excision ◽  
Related Disease ◽  
Cytological Features

Inflamed conjunctival nevi (ICN) may suggest malignancy because of their rapid growth and atypical histology. The objective of this study was to characterize the diagnostic features of ICN. A retrospective, nonrandomized study of 13 patients with ICN was conducted. A scoring method was developed based on histology and immunopathological parameters. The presence of epithelial solid or cystic inclusions and the preservation of goblet cells are consistent with a benign melanocytic lesion. Periodic acid–Schiff stain and immunohistochemistry to identify the epithelial component prove helpful in the differential diagnosis for melanoma. Polyclonal lymphoid infiltrate and benign cytological features of ICN exclude a diagnosis of lymphoma. Despite the presence of immunoglobulin 4–positive plasma cells in the lesions, ICN does not meet the diagnostic criteria for immunoglobulin 4–related disease. Most patients with ICN are young. The treatment for ICN is complete excision, and the prognosis is excellent.

Ultrastructure of the eta cells in the pituitary gland of adult migratory sockeye salmon (Oncorhynchus nerka)

1969 ◽  
Vol 47 (5) ◽  
pp. 937-941 ◽  
Author(s):  
H. Cook ◽  
A. P. van Overbeeke
Keyword(s):  
Endoplasmic Reticulum ◽  
Pituitary Gland ◽  
Sockeye Salmon ◽  
Secretory Granules ◽  
Oncorhynchus Nerka ◽  
Pars Distalis ◽  
Cell Type ◽  
Spawning Grounds ◽  
Predominant Cell Type ◽  
Rostral Pars Distalis

In the rostral pars distalis of the adult migratory sockeye salmon (Oncorhynchus nerka), most of the cells are arranged in follicles. The predominant cell type, the eta cell, contains secretory granules of 175–300 mμ in diameter; its endoplasmic reticulum shows a conspicuous lamellar organization. These cells undergo only slight changes as the fish migrate from the ocean to the spawning grounds. The findings are discussed in relation to the function of prolactin in euryhaline teleosts.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Detection of Carriers in Glanzmann's Thrombasthenia

1983 ◽  
Vol 49 (03) ◽  
pp. 182-186
Author(s):  
G T E Zonneveld ◽  
E F van Leeuwen ◽  
A Sturk ◽  
J W ten Cate
Keyword(s):  
Bleeding Time ◽  
Periodic Acid ◽  
Type I ◽  
Clot Retraction ◽  
Periodic Acid Schiff ◽  
Glanzmann’S Thrombasthenia ◽  
Aggregation Response ◽  
Glanzmann's Thrombasthenia ◽  
Sds Page ◽  
Reduced State

SummaryQuantitative glycoprotein (GP) analysis of whole platelets or platelet membranes was performed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) and periodic acid Schiff staining in the families of two unrelated Glanzmann’s thrombasthenia (GT) patients. Each family consisted of two symptom free parents, a symptom free daughter and a GT daughter. All symptom free members had a normal bleeding time, clot retraction and platelet aggregation response to adenosine 5’-diphosphate (ADP), collagen and adrenalin. Platelet Zw* antigen was normally expressed in these subjects. GT patiens, classified as a type I and II subject, showed reduced amounts of GP lib and of GP nia. Analysis of isolated membranes in the non-reduced state, however, showed that the amount of GP Ilia was also reduced in three of the four parents, whereas one parent (of the GT type I patient) and the two unaffected daughters had normal amounts of GP Ilia. Quantitative SDS-PAGE may therefore provide a method for the detection of asymptomatic carriers in GT type I and II.

Swim bladder mycosis in farmed rainbow trout Oncorhynchus mykiss caused by Phoma herbarum and experimental verification of pathogenicity

2020 ◽  
Vol 138 ◽  
pp. 237-246 ◽  
Author(s):  
J Řehulka ◽  
A Kubátová ◽  
V Hubka
Keyword(s):  
Rainbow Trout ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Bladder Wall ◽  
Swim Bladder ◽  
Cell Reactivity ◽  
Periodic Acid Schiff ◽  
Posterior Portion ◽  
Hepatic Congestion ◽  
Phoma Herbarum

In this study, spontaneous swim bladder mycosis was documented in a farmed fingerling rainbow trout from a raceway culture system. At necropsy, the gross lesions included a thickened swim bladder wall, and the posterior portion of the swim bladder was enlarged due to massive hyperplasia of muscle. A microscopic wet mount examination of the swim bladder contents revealed abundant septate hyphae, and histopathological examination showed periodic acid-Schiff-positive mycelia in the lumen and wall of the swim bladder. Histopathological examination of the thickened posterior swim bladder revealed muscle hyperplasia with expansion by inflammatory cells. The causative agent was identified as Phoma herbarum through morphological analysis and DNA sequencing. The disease was reproduced in rainbow trout fingerlings using intraperitoneal injection of a spore suspension. Necropsy in dead and moribund fish revealed extensive congestion and haemorrhages in the serosa of visceral organs and in liver and abdominal serosanguinous fluid. Histopathological examination showed severe hepatic congestion, sinusoidal dilatation, Kupffer cell reactivity, leukostasis and degenerative changes. Fungi were disseminated to the liver, pyloric caeca, kidney, spleen and heart. Although infections caused by Phoma spp. have been repeatedly reported in fish, species identification has been hampered by extensive taxonomic changes. The results of this study confirmed the pathogenicity of P. herbarum in salmonids by using a reliably identified strain during experimental fish infection and provides new knowledge regarding the course of infection.

Erdheim-Chester Disease Presenting with Histiocytic Colitis and Cytokine Storm

2017 ◽  
Vol 26 (2) ◽  
pp. 183-187
Author(s):  
George P. Christophi ◽  
Yeshika Sharma ◽  
Quader Farhan ◽  
Umang Jain ◽  
Ted Walker ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Poor Response ◽  
Rare Type ◽  
Periodic Acid Schiff ◽  
Hematopoietic Stem ◽  
Hemodynamic Compromise ◽  
Erdheim Chester Disease ◽  
Erdheim Chester ◽  
Chester Disease ◽  
Segmental Colitis

Background: Non-Langerhans histiocytosis is a group of inflammatory lymphoproliferative disorders originating from non-clonal expansion of hematopoietic stem cells into cytokine-secreting dendritic cells or macrophages. Erdheim-Chester Disease (ECD) is a rare type of non-Langerhans cell histiocytosis characterized by tissue inflammation and injury caused by macrophage infiltration and histologic findings of foamy histiocytes. Often ECD involves the skeleton, retroperitoneum and the orbits. This is the first report documenting ECD manifesting as segmental colitis and causing cytokine-release syndrome.Case presentation: A 68-year old woman presented with persistent fever without infectious etiology and hematochezia. Endoscopy showed segmental colitis and pathology revealed infiltration of large foamy histiocytes CD3-/CD20-/CD68+/CD163+/S100- consistent with ECD. The patient was empirically treated with steroids but continued to have fever and developed progressive distributive shock.Conclusion: This case report describes the differential diagnosis of infectious and immune-mediated inflammatory and rheumatologic segmental colitis. Non-Langerhans histiocytosis and ECD are rare causes of gastrointestinal inflammation. Prompt diagnosis is imperative for the appropriate treatment to prevent hemodynamic compromise due to distributive shock or gastrointestinal bleeding. Importantly, gastrointestinal ECD might exhibit poor response to steroid treatment and other potential treatments including chemotherapy, and biologic treatments targeting IL-1 and TNF-alpha signaling should be considered.Abbreviations: AFB: acid-fast bacilli; ECD: Erdheim-Chester Disease; IBD: inflammatory bowel disease; PASD: periodic acid-Schiff with diastase; TB: tuberculosis

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