scholarly journals Effect of 5-azacytidine induced DNA demethylation on abiotic stress tolerance in Arabidopsis thaliana

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 73-80 ◽  
Author(s):  
Zlata V. Ogneva ◽  
Andrey R. Suprun ◽  
Alexandra S. Dubrovina ◽  
Konstantin V. Kiselev
Keyword(s):  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Survival Rates ◽  
Biomass Accumulation ◽  
Dna Demethylation ◽  
Freezing Stress ◽  
Dna Hypomethylation ◽  
Inducible Genes

The effect of 5-azacytidine (5A)-induced DNA hypomethylation on the growth and abiotic stress tolerance of Arabidopsis thaliana were analysed. Growth analysis revealed that aqueous solutions of 5A added to the soil did not affect the fresh and dry biomass accumulation but led to a higher percentage of flowering A. thaliana plants after four weeks of cultivation. The 5A treatment considerably lowered survival rates of Arabidopsis plants under high soil salinity, heat stress, and drought, while it did not affect the survival rates after freezing stress. 5A eliminated the stimulatory effect of the heat and drought stresses on the transcriptional levels of a number of stress-inducible genes, such as DREB1, LEA, SOS1, or RD29A. A less clear but similar trend has been detected for the effect of 5A on expression of the stress-inducible genes under salt and cold stresses. The data indicate that DNA methylation is an important mechanism regulating plant abiotic stress resistance.

scholarly journals Overexpression of a Novel ROP Gene from the Banana (MaROP5g) Confers Increased Salt Stress Tolerance

2018 ◽  
Vol 19 (10) ◽  
pp. 3108 ◽  
Author(s):  
Hongxia Miao ◽  
Peiguang Sun ◽  
Juhua Liu ◽  
Jingyi Wang ◽  
Biyu Xu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Survival Rates ◽  
Wild Type ◽  
Ion Distribution ◽  
Membrane Injury ◽  
Primary Roots

Rho-like GTPases from plants (ROPs) are plant-specific molecular switches that are crucial for plant survival when subjected to abiotic stress. We identified and characterized 17 novel ROP proteins from Musa acuminata (MaROPs) using genomic techniques. The identified MaROPs fell into three of the four previously described ROP groups (Groups II–IV), with MaROPs in each group having similar genetic structures and conserved motifs. Our transcriptomic analysis showed that the two banana genotypes tested, Fen Jiao and BaXi Jiao, had similar responses to abiotic stress: Six genes (MaROP-3b, -5a, -5c, -5f, -5g, and -6) were highly expressed in response to cold, salt, and drought stress conditions in both genotypes. Of these, MaROP5g was most highly expressed in response to salt stress. Co-localization experiments showed that the MaROP5g protein was localized at the plasma membrane. When subjected to salt stress, transgenic Arabidopsis thaliana overexpressing MaROP5g had longer primary roots and increased survival rates compared to wild-type A. thaliana. The increased salt tolerance conferred by MaROP5g might be related to reduced membrane injury and the increased cytosolic K+/Na+ ratio and Ca2+ concentration in the transgenic plants as compared to wild-type. The increased expression of salt overly sensitive (SOS)-pathway genes and calcium-signaling pathway genes in MaROP5g-overexpressing A. thaliana reflected the enhanced tolerance to salt stress by the transgenic lines in comparison to wild-type. Collectively, our results suggested that abiotic stress tolerance in banana plants might be regulated by multiple MaROPs, and that MaROP5g might enhance salt tolerance by increasing root length, improving membrane injury and ion distribution.

scholarly journals Heterologous Expression of the Wheat Aquaporin Gene TaTIP2;2 Compromises the Abiotic Stress Tolerance of Arabidopsis thaliana

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79618 ◽  
Author(s):  
Chunhui Xu ◽  
Meng Wang ◽  
Li Zhou ◽  
Taiyong Quan ◽  
Guangmin Xia
Keyword(s):  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Heterologous Expression ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Aquaporin Gene

Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana

2013 ◽  
Vol 83 (3) ◽  
pp. 265-277 ◽  
Author(s):  
Telma E. Scarpeci ◽  
María I. Zanor ◽  
Bernd Mueller-Roeber ◽  
Estela M. Valle
Keyword(s):  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Early Growth ◽  
Growth Stages
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