Effects of Increased Dietary Protein and Energy on Composition and Functional Capacities of Blood Mononuclear Cells from Vaccinated, Neonatal Calves

2005 ◽  
Vol 75 (5) ◽  
pp. 357-368 ◽  
Author(s):  
Foote ◽  
Nonnecke† ◽  
Waters ◽  
Palmer ◽  
Beitz ◽  
...  
Keyword(s):  
Weight Gain ◽  
B Cell ◽  
Dietary Protein ◽  
Crude Protein ◽  
Interleukin 2 ◽  
Milk Replacer ◽  
Standard Diet ◽  
Gamma Delta ◽  
Ifn Gamma ◽  
Functional Capacities

Effects of increased protein and energy provided by an intensified milk replacer on the antigen-specific, cell-mediated immune response of the neonatal calf were examined. Calves were fed a standard (0.45 kg/day of a 20% crude protein, 20% fat milk replacer; n = 11) or intensified (1.14 kg/day of a 28% crude protein, 20% fat milk replacer; n = 11) diet from 0 to 6 weeks of age. All calves were vaccinated with Mycobacterium bovis bacillus Calmette-Guerin (BCG) at 1 week of age. The daily weight gain of intensified-diet calves (0.62 kg/day) was greater than the weight gain of standard-diet calves (0.29 kg/day). Liver, kidney, heart, thymus, and subcervical lymph nodes from intensified-diet calves were heavier than the same organs from standard-diet calves. Flow cytometric analysis of peripheral blood mononuclear cell (PBMC) populations indicated that CD4+ cells, gamma delta TCR+ cells, and monocyte percentages, although unaffected by diet during the first 5 weeks of the study, were higher in intensified-diet calves at week 6. The decline in gamma deltad TCR+ cell percentages and increase in B cell percentages with increasing age seen in all calves are characteristic of the maturing immune system of the calf. CD8+ T cell or B cell percentages were not affected by diet. In intensified-diet calves, percentages of CD4+ expressing interleukin-2 receptor increased and percentages of gamma delta TCR+ cells expressing interleukin-2 receptor decreased with time. The same populations in standard-diet calves did not change with time. Percentages of CD4+ and CD8+ T cells, and B cells expressing MHC class II antigen, were unaffected by diet or age. Although mitogen-induced interferon (IFN)-gamma and nitric oxide (NO) secretion increased with age for all calves, PBMC from intensified-diet calves produced less IFN-gamma and more NO than did cells from standard-diet calves at week 6 of the study. Antigen-induced secretion of IFN-gamma and NO also increased with age but was unaffected by diet. Antigen-elicited delayed-type hypersensitivity was unaffected by diet, suggesting increased dietary protein and energy did not alter adaptive immunity in vivo. Overall, these results suggest that feeding calves a commercially available, intensified milk replacer affects minimally the composition and functional capacities of PBMC populations. Additional research is necessary to determine whether these subtle effects influence the calf’s susceptibility to infectious disease.

scholarly journals Growth and hematology of pacu subjected to sustained swimming and fed different protein levels

2013 ◽  
Vol 48 (6) ◽  
pp. 645-650 ◽  
Author(s):  
Cleujosí da Silva Nunes ◽  
Gilberto Moraes ◽  
Fernando Fabrizzi ◽  
Araceli Hackbarth ◽  
Gustavo Alberto Arbeláez-Rojas
Keyword(s):  
Growth Rate ◽  
Weight Gain ◽  
Specific Growth Rate ◽  
Dietary Protein ◽  
Crude Protein ◽  
Specific Growth ◽  
Daily Weight Gain ◽  
Mean Corpuscular Hemoglobin ◽  
Protein Levels ◽  
Randomized Design

The objective of this work was to evaluate the effect of sustained swimming and dietary protein levels on growth and hematological responses of juvenile pacu (Piaractus mesopotamicus). A completely randomized design was used in a 3x2 factorial arrangement, with three levels of dietary protein (24, 28, and 32% crude protein), two rearing conditions (sustained swimming or motionless water), and 15 replicates. Fish were subjected to sustained swimming at the velocity of two body lengths per second (2 BL s-1), for 45 days. The level of dietary protein and the swimming conditions affected the performance, growth, and hematological profile of pacu. Swimming conditions influenced nutritional factors, increasing daily weight gain, specific growth rate, number of erythrocytes, mean corpuscular volume, and mean corpuscular hemoglobin. Fish under sustained swimming and fed with 24% crude protein showed better growth performance, with higher specific growth rate (4.11±0.88) and higher daily weight gain (2.19±0.47 g per day). Sustained swimming can increase the productive performance of pacu and simultaneously reduce dietary protein levels.

Sucrose-induced obesity: effect of diet on obesity and brown adipose tissue

1987 ◽  
Vol 253 (1) ◽  
pp. R158-R166 ◽  
Author(s):  
R. B. Kanarek ◽  
J. R. Aprille ◽  
E. Hirsch ◽  
L. Gualtiere ◽  
C. A. Brown
Keyword(s):  
Adipose Tissue ◽  
Weight Gain ◽  
Brown Adipose Tissue ◽  
Dietary Protein ◽  
Feed Efficiency ◽  
Protein Diet ◽  
Standard Diet ◽  
Metabolic Potential ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose

Adult male Sprague-Dawley rats were divided into three groups and fed diets containing either 10, 20, or 40% protein for 56 days. Half of the rats in each dietary condition were given a 32% sucrose solution plus the standard diet and water. Sucrose intake varied directly as a function of dietary protein levels. Rats fed either the 10 or 20% protein diet and sucrose had higher caloric intakes, gained more weight, were more efficient at using calories for weight gain, and had more adipose tissue than rats given the same diet without sucrose. Rats fed the 40% protein diet and sucrose did not exhibit overeating, excess weight gain, or increased feed efficiency relative to animals fed the 40% diet alone. Animals given sucrose had more interscapular brown adipose tissue (IBAT) and a greater metabolic potential for thermogenesis in IBAT as determined by GDP binding in mitochondria than rats not fed sucrose. These results demonstrate that dietary protein is important in the development of sucrose-induced obesity and that increases in IBAT mass and activity can occur concomitant with increased feed efficiency.

scholarly journals Gut Histomorphometric and Blood Profile of Broiler Chicks Fed Varied Levels Of Protein

2020 ◽  
pp. 106-111
Author(s):  
Shittu, M. D ◽  
Adejumo, D. O, ◽  
Ademola, S. G, ◽  
Alagbe, J.O
Keyword(s):  
Dietary Protein ◽  
Crude Protein ◽  
Early Stage ◽  
Protein Digestibility ◽  
Active Role ◽  
Broiler Chicks ◽  
Standard Diet ◽  
Blood Profile ◽  
Protein Levels ◽  
Dietary Treatments

Gut plays active role in screening what goes into the blood stream and blood is very important parameter to access for us to establish the health status of animal to any dietary variation, this will help the producer ability to guaranty the consumers’ health. Therefore this research was designed to determine gut histomorphometry and blood profile of broiler starter fed varied levels of protein. Material and method is A total of 144 days-old-broiler chicks were used for the study. Broiler standard starter mash with varied protein percentages of 20, 23, and 26%, respectively for treatments 1, 2 and 3 were used. Data were analysed using ANOVA at α0.05. Results is Except crude protein and ash contents, others nutrient digestibilities were not affected by the dietary treatments. Birds fed lower crude protein had the lowest crude protein digestibility while birds fed standard diet (23% CP) had the best and highest crude protein digestibility. White blood cell decrease with decrease dietary protein, this might have serious effect on the immunity level of the broilers. Also globulin increases while albumin decreases with increase in dietary protein levels. Serum total protein increases with increase in the dietary protein levels and diet with26% CP recorded the highest value (5.82g/dL). Serum cholesterol values were significantly higher at lower protein level unlike serum protein whiledifferent gut parts response significantly different to dietary treatments. Conclusion is at early stage broiler growth, diet need to have higher CP to improve the immunity and health growth of gut.

scholarly journals Interferon-gamma gene expression in human B-cell lines: induction by interleukin-2, protein kinase C activators, and possible effect of hypomethylation on gene regulation

Blood ◽  
1992 ◽  
Vol 80 (3) ◽  
pp. 724-732 ◽  
Author(s):  
Y Pang ◽  
Y Norihisa ◽  
D Benjamin ◽  
RR Kantor ◽  
HA Young
Keyword(s):  
Gene Expression ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Interleukin 2 ◽  
Cell Types ◽  
Chain Gene ◽  
Ifn Gamma ◽  
Kinase C ◽  
Human B Cell

Human interferon-gamma (IFN-gamma) is an important immunomodulatory protein produced predominantly by T cells and large granular lymphocytes (LGLs). Whereas large amounts of data have been accumulated regarding IFN gamma gene expression in these two cell types, little information about IFN gamma expression in other cell types exists. In this study, we have analyzed the production of IFN gamma by the Epstein- Barr virus (EBV)-positive B-cell line, JLP(c), derived from a patient with Burkitt's lymphoma, and another human B-cell line, PA682BM-1, which was derived from an acquired immunodeficiency syndrome patient. Southern blot analysis indicates the presence of an Ig heavy chain gene rearrangement, but no rearrangement of the T-cell receptor beta chain gene or IFN gamma gene in these B-cell lines. Both cell lines were found to express surface IgD and other B-cell surface markers, thus confirming their B-cell lineage. Analysis for surface Ig, cytoplasmic Ig, and secreted Ig indicates that the two cell lines are in relatively early stages of the B-cell differentiation pathway. We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). After activation of the cell lines with IL-2, (-)Indolactam-v, and PMA, increases in cytoplasmic messenger RNAs (mRNAs) of IFN gamma and the IL- 2 receptor chains were also observed. The induction of IFN gamma mRNA and protein by IL-2 was completely blocked by a monoclonal antibody to IL-2 receptor p75 (beta chain), but not by the monoclonal antibody to p55 (alpha chain). Analysis of IFN gamma genomic DNA indicates that the gene is not amplified, but that hypomethylation in the 5′ noncoding region of the IFN gamma gene has occurred in the B-cell line from the Burkitt's lymphoma patient that spontaneously produces IFN gamma. This finding suggests that the methylation state of the promoter region may play an important role in the control of IFN gamma gene expression in B cells.

scholarly journals Meat production of young and adult Kejobong goats fed diets with different protein concentration

2021 ◽  
Vol 31 (1) ◽  
pp. 52-59
Author(s):  
Nuning Ari Purnami ◽  
Endang Purbowati ◽  
Edy Rianto
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Dietary Protein ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Concentration ◽  
Body Weight Gain ◽  
Dry Matter Intake ◽  
Meat Production ◽  
Dietary Crude Protein

This study was aimed to examine the effect of dietary crude protein (CP) concentration on carcass and meat production in young and adult Kejobong goats. This study used 8 young (5 months) and 8 adult (9 months) Kejobong goats; which were allocated to a randomized nested design, with an age of goat as the nest, and treatment applied was dietary protein concentration (T1: 9.96%; T2: 14.66%). The parameters observed were dry matter intake (DMI), CP intake (CPI), body weight gain (BWG), and the production of carcass meat. The results showed that the DMI of adult goats (910 g/d) was higher (P<0.05) than that of young goats (680 g/d). The goats fed diet T2 had higher (P<0.05) DMI, CPI, and BWG (855 g, 140 g, and 94 g/d, respectively) than those of T1 (725 g/d, 80 g/d, and 59 g/d). Slaughter weight, carcass weight and carcass meat weight, and carcass meat percentage of adult goats (28.09 kg, 13.19 kg, 8.60 kg and 64.94%, respectively) were higher (P <0.05) than those of young goats (20.06 kg, 8.72 kg, 5.46 kg, and 62.06%, respectively). The weight and percentage of carcass meat of T2 (8.30 kg and 64.99%, respectively) were higher (P<0.05) than those of T1 (5.75 kg and 62.06%, respectively). The meat-bone ratio of adult goats was higher (P<0.05) than that of young goats (3.53 vs 2.85). The meat-bone ratio of T2 (3.58) was higher (P<0.05) than that of T1 (2.80). It was concluded that adult Kejobong goats produced more meat than the young ones, and the goat-fed diet with 14.66% CP produced more meat than that with 9.96% CP.

scholarly journals Interleukin 2 (IL 2) and interferon-gamma production by T lymphocytes from patients with B-chronic lymphocytic leukemia: evidence that normally released IL 2 is absorbed by the neoplastic B cell population

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 614-619 ◽  
Author(s):  
R Foa ◽  
M Giovarelli ◽  
C Jemma ◽  
MT Fierro ◽  
P Lusso ◽  
...  
Keyword(s):  
B Cells ◽  
Chronic Lymphocytic Leukemia ◽  
T Lymphocytes ◽  
B Cell ◽  
T Lymphocyte ◽  
Interleukin 2 ◽  
Lymphocytic Leukemia ◽  
Ifn Gamma ◽  
Culture Supernatants

Abstract The capacity of T lymphocytes from patients with B cell chronic lymphocytic leukemia (B-CLL) to release interleukin 2 (IL 2) and interferon (IFN)-gamma was assessed following various stimuli. The spontaneous release of IL 2 and IFN-gamma was practically absent both with B-CLL and normal T lymphocytes. By contrast, after stimulation with phytohemagglutinin (PHA) or with PHA plus 12-O- tetradecanoylphorbol-13-acetate, the production of IL 2 and IFN-gamma by B-CLL T lymphocytes was similar to that of normal T lymphocytes, irrespective of the reversed T lymphocyte subset distribution (OKT4/OKT8 ratio) observed in B-CLL. However, the titer of IL 2 was greatly reduced when autologous leukemic B cells were added to the culture system. Unlike IL 2, the presence of leukemic B cells did not affect the titer of IFN-gamma in the culture supernatants. The indication that IL 2 may be adsorbed in vivo by the neoplastic B cells was further confirmed by the demonstration of the IL 2 receptor (revealed by anti-Tac monoclonal antibody) on the leukemic B cells, particularly following mitogenic stimulation, and by the evidence that exogenous IL 2 can be directly absorbed by untreated B-CLL T lymphocytes to release IFN-gamma and IL 2 is preserved, but that IL 2 may be rapidly removed by the neoplastic B-CLL cells, thus contributing to the well-documented T lymphocyte abnormalities present in this disease.

scholarly journals Interferon-gamma gene expression in human B-cell lines: induction by interleukin-2, protein kinase C activators, and possible effect of hypomethylation on gene regulation

Blood ◽  
1992 ◽  
Vol 80 (3) ◽  
pp. 724-732 ◽  
Author(s):  
Y Pang ◽  
Y Norihisa ◽  
D Benjamin ◽  
RR Kantor ◽  
HA Young
Keyword(s):  
Gene Expression ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Interleukin 2 ◽  
Cell Types ◽  
Chain Gene ◽  
Ifn Gamma ◽  
Kinase C ◽  
Human B Cell

Abstract Human interferon-gamma (IFN-gamma) is an important immunomodulatory protein produced predominantly by T cells and large granular lymphocytes (LGLs). Whereas large amounts of data have been accumulated regarding IFN gamma gene expression in these two cell types, little information about IFN gamma expression in other cell types exists. In this study, we have analyzed the production of IFN gamma by the Epstein- Barr virus (EBV)-positive B-cell line, JLP(c), derived from a patient with Burkitt's lymphoma, and another human B-cell line, PA682BM-1, which was derived from an acquired immunodeficiency syndrome patient. Southern blot analysis indicates the presence of an Ig heavy chain gene rearrangement, but no rearrangement of the T-cell receptor beta chain gene or IFN gamma gene in these B-cell lines. Both cell lines were found to express surface IgD and other B-cell surface markers, thus confirming their B-cell lineage. Analysis for surface Ig, cytoplasmic Ig, and secreted Ig indicates that the two cell lines are in relatively early stages of the B-cell differentiation pathway. We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). After activation of the cell lines with IL-2, (-)Indolactam-v, and PMA, increases in cytoplasmic messenger RNAs (mRNAs) of IFN gamma and the IL- 2 receptor chains were also observed. The induction of IFN gamma mRNA and protein by IL-2 was completely blocked by a monoclonal antibody to IL-2 receptor p75 (beta chain), but not by the monoclonal antibody to p55 (alpha chain). Analysis of IFN gamma genomic DNA indicates that the gene is not amplified, but that hypomethylation in the 5′ noncoding region of the IFN gamma gene has occurred in the B-cell line from the Burkitt's lymphoma patient that spontaneously produces IFN gamma. This finding suggests that the methylation state of the promoter region may play an important role in the control of IFN gamma gene expression in B cells.

EFFECT OF DIFFERENT LEVELS OF DIETARY PROTEIN ON PLASMA 5α-ANDROSTENONE CONCENTRATIONS IN YOUNG BOARS

1980 ◽  
Vol 60 (4) ◽  
pp. 1019-1022 ◽  
Author(s):  
V. R. GOWAN ◽  
R. NARENDRAN ◽  
R. J. ETCHES
Keyword(s):  
Weight Gain ◽  
Dietary Protein ◽  
Crude Protein ◽  
Protein Concentration ◽  
Plasma Concentrations ◽  
Protein Levels ◽  
Young Boars ◽  
Protein Diets ◽  
Different Levels ◽  
Effect Of Feeding

Nineteen individually-penned Yorkshire boars, averaging 23 kg, were used to evaluate the effect of feeding 15, 17, 19 and 21% crude protein diets on plasma 5α-androstenone concentrations up to 100 kg liveweight. Although the protein levels significantly (P < 0.01) affected cumulative weight gain, the results indicated that the dietary protein concentration had no effect on plasma concentrations of 5α-androstenone.

The effect of the dietary protein restriction and re-feeding on the content of leptin, IGF-I and urea nitrogen in the blood plasma and growth performance in pigs

2018 ◽  
Vol 58 (6) ◽  
pp. 1119 ◽  
Author(s):  
M. Brestenský ◽  
S. Nitrayová ◽  
A. V. Sirotkin ◽  
P. Patráš ◽  
J. Heger
Keyword(s):  
Growth Performance ◽  
Dietary Protein ◽  
Crude Protein ◽  
Protein Restriction ◽  
Growing Pigs ◽  
Standard Diet ◽  
Feeding Period ◽  
Urea Nitrogen ◽  
Dietary Protein Restriction ◽  
Igf I

The aim of the present study was to examine the effect of the dietary protein restriction on the plasma concentrations of leptin, insulin-like growth factor (IGF-I), blood urea nitrogen (BUN) and growth performance in growing pigs. A total of 12 gilts were divided into experimental (ET) and control (CT) treatments. After the 14-day dietary restriction period during which the ET fed a low-protein diet (LPD, 5% of crude protein) and the CT fed a standard diet (SD, 16% of crude protein) the second 14-day re-feeding period followed, in which LPD was replaced by a SD that was fed by the both treatments. During the dietary protein restriction reduced (P < 0.05) N intake, average daily gain (ADG), concentration of BUN and tendency (P = 0.084) to the lower IGF-I in ET compared with CT were observed. Feed to gain ratio was greater (P < 0.05) in ET. During the subsequent re-feeding period, there was a tendency to the greater daily feed intake (P = 0.068) and N intake (P = 0.070), greater (P < 0.05) ADG, BUN and plasma leptin but no IGF-I in ET. These observations suggest that dietary protein restriction promotes body growth in pigs, which is associated with increase in blood leptin and BUN level in ET during the re-feeding, indicating that the increased ADG can be due to increased fat deposition but not of protein synthesis.

scholarly journals Growth, survival and nutrient utilization of the brackish water catfish (Chrysicthys walkeri) fry as affected by dietary protein and energy levels

2021 ◽  
Vol 31 (1) ◽  
pp. 144-150
Author(s):  
S. O. Obasa
Keyword(s):  
Weight Gain ◽  
Dietary Protein ◽  
Brackish Water ◽  
Crude Protein ◽  
Specific Growth ◽  
Energy Levels ◽  
Nutrient Utilization ◽  
Conversion Ratio ◽  
Feed Conversion ◽  
A Value

The effect of varying dietary protein and calorie levels on the growth, survival and nutrient utilization of 540 brackish water catfish (Chrysicthys walkeri) fry average liveweight of 0.14g was investigated in a 3x3 factorial experiment with 3 calorie levels ( 2800, 3000 and 3200 Kcal ME/Kg diet) and 3 levels of crude protein (36%, 39% and 42%) giving a total of 9 diets, fed to the fish for a period of 56 days. Mean weight gain, specific growth rate, feed conversion ratio, survival and protein productive value were significantly higher (P<0.05) in fish fed diet 6 (3000 Kcal ME/Kg and 42% crude protein) when compared to other diets. Weight gain was highest in diet 6 with a value of 0.64g and lowest (significantly P<0.05) with a value of 71.67% and lowest in diet 1 (2800Kcal ME/Kg and 36% crude protein) with a value of 53.34%, while protein productive value was higher in diet 6 (78.40) and lowest in diet (2800Kcal ME/Kg, 39% crude protein) with a value of value of 64.33%. Thus, C. walkeri for survived and performed best when fed on 42% crude protein and 3000Kcal ME/kg diet.  

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