scholarly journals Race and Virulence Determination of Fusarium oxysporum f. sp. cubense Isolates from Sidomulyo Village of Bantul, Yogyakarta

2018 ◽  
Vol 22 (1) ◽  
pp. 72
Author(s):  
Herika Novrelly Jayatri ◽  
Christanti Sumardiyono ◽  
Arif Wibowo

Banana is one of the important fruit crop in Village of Sidomulyo, Bantul, Yogyakarta. One of important diseases which become the constraint in development of banana is Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). This fungus has high race diversity and  virulence, so that it required early detection for prevention and control of disease. This experiment was aimed to figure out race and virulence of Foc isolates from Village of Sidomulyo, Bantul, Yogyakarta. The 13 tested isolates were isolates of PR11, PKJ20, RU20, PR30, AH40, PKJ40, A41, RB42, PR43, RU51, A60, RP60, and A80. Race was molecularly detected using two types of primers, i.e. General Foc primer FocEf3 and specific primer for race 4 (Foc-1/Foc-2). Virulence test was performed on banana seedlings of Ambon Kuning cultivar using Completely Randomized Design (CRD) with 14 treatments and 4 repetitons. The observed parameters were external and internal symptoms, calculation of disease severity index and disease intensity. Data were analyzed using variance and further test of Duncan Multiple Range Test (DMRT) at 5 % level. The results showed that all isolates were Foc and 9 of 13 isolates were grouped into race 4, i.e. A80, RP60, PR11, A41, AH40, PKJ40, PR30, RB42, and PR43. The highest and lowest virulences were consecutively expressed by PR30, RB42, RU51, RP60, PR43, PKJ40, PR11, A41, AH40, RU20, PKJ20, A60, and A80, with severity index on leaves and rhizomes ranging 1.61-2.91 and 2.25-7, respectively. IntisariPisang merupakan tanaman buah unggulan di Desa Sidomulyo Kecamatan Bambanglipuro, Kabupaten Bantul. Salah satu penyakit penting yang menjadi kendala dalam pengembangan pisang adalah layu fusarium yang disebabkan oleh jamur Fusarium oxysporum f. sp. cubense (Foc). Jamur ini memiliki keragaman ras dan virulensi yang tinggi, sehingga deteksi dini diperlukan untuk pencegahan dan pengendalian penyakit. Penelitian ini bertujuan untuk mengetahui ras dan virulensi isolat Foc asal Desa Sidomulyo, Kecamatan Bambanglipuro, Kabupaten Bantul. Isolat yang diuji sebanyak 13 isolat, yakni isolat PR11, PKJ20, RU20, PR30, AH40, PKJ40, A41, RB42, PR43, RU51, A60, RP60, dan A80. Pengujian ras secara molekuler dengan menggunakan dua jenis primer yakni primer Foc in general FocEf3 dan primer spesifik ras 4 Foc-1/Foc-2. Uji virulensi pada bibit kultivar ambon kuning dengan menggunakan Racangan Acak Lengkap (RAL) yang terdiri dari 14 perlakuan dan 4 ulangan. Parameter yang diamati berupa pengamatan gejala luar dan gejala dalam, penghitungan indeks keparahan penyakit dan intensitas penyakit. Analisis data menggunakan sidik ragam dan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf 5 %. Hasil pengujian menunjukkan bahwa semua isolat merupakan isolat Foc dan dari 13 isolat yang digunakan terdapat 9 isolat yang merupakan ras 4 yakni isolat A80, RP60, PR11, A41, AH40, PKJ40, PR30, RB42, dan PR43. Isolat yang memiliki virulensi tertinggi sampai terendah berturut-turut adalah PR30, RB42, RU51, RP60, PR43, PKJ40, PR11, A41, AH40, RU20, PKJ20, A60, dan A80, dengan indeks keparahan pada daun berkisar 1,61−2,91 dan indeks keparahan pada bonggol 2,25−7.

2016 ◽  
Vol 16 (2) ◽  
pp. 69
Author(s):  
Aprilian Adi Nugroho ◽  
Sri Sumarsih ◽  
Bambang Sulistiyanto

ABSTRAK. Penelitian efek penambahan bentonit dalam proses pelleting terhadap total bakteri dan total fungi dari produk pellet limbah penetasan sebagai bahan pakan alternatif, telah dilaksanakan di Laboratorium Teknologi Pakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro. Penambahan bentonit pada proses pelleting diharapkan mengurangi total bakteri dan total fungi pada pellet olahan limbah penetasan, sehingga aman digunakan sebagai bahan pakan alternatif. Pada penelitian ini limbah penetasan yang berupa cangkang telur, telur infertile, telur gagal menetas, serta DOC afkir dan mati dihancurkan, dicampur dengan bentonit dan dibuat pellet, untuk kemudian dianalisis kandungan total bakteri dan total fungi pada produk pellet. Perlakuan bentonit dilakukan dengan aras 0, 2, 4 dan 6% (B/B). Penelitian dilakukan dengan rancangan acak lengkap (RAL) 4 perlakuan dan 5 ulangan. Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji wilayah ganda Duncan untuk mengetahui perbedaan antar perlakuan. Hasil menunjukkan bahwa pemberian bentonit dari berbagai level berpengaruh sangat nyata (P<0,01). Pemberian bentonit pada tingkat tertinggi (6%) mampu menekan total bakteri dan total fungi pada kisaran 105 cfu/g. Disimpulkan bahwa bentonit mampu menekan kandungan total bakteri dan total fungi pada produk pellet limbah penetasan. (Numbers of total bacteria and total fungi of hatchery waste pellets that made by adding bentonite) ABSTRACT. A Research about the effect of bentonite addition in the pelletizing process on total bacteria and total fungi of pellet product of hatchery waste as an alternative feedstuff, was done at Feed Technology Laboratory, Faculty of Animal and Agricultural Sciences, Diponegoro University. The addition of bentonite was expected to reduce the number of bacteria and fungi in the pellet product of hatchery waste, therefore, it could be used safely as an alternative feedstuff. In this experiment, the hatchery waste in the forms of eggshell, infertile eggs, un-hatched eggs, dead and culled DOC were blended, mixed with bentonite and pelleted,and then the number of total bacteria and total fungi of pellet was analyzed. The treatment was carried out with the level of bentonite i.e. 0, 2, 4 and 6% (w/w). Completely randomized design (CRD)was applied by 4 treatments and 5 replications of each. Data analyzed by analysis of variance (ANOVA), and followed by Duncan multiple range test (DMRT) to determine differences among the treatments. The results showed that administration of bentonite at various levels significantly affect the number of bacteria and fungi in the hatchery waste pellets (P <0.01). Administration of bentonite at the level of 6% shows the highest value on suppressing the content of total bacteria as well as fungi up to the range of total content 105cfu/g. In conclusion, the bentonite was able to suppress the total amount of bacteria and total fungi in the hatchery waste pellets.


2018 ◽  
Vol 18 (2) ◽  
pp. 90-94
Author(s):  
Dedhi Yustendi ◽  
Ainal Mardhiah

ABSTRAK. Penelitian dilaksanakan pada kelompok ternak kambing perah di Desa Geucee Kayee Jatoe Kota Banda Aceh. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan persentase pemberian ekstrak daun katuk dalam air minum yaitu S0, S1, S2 dan S3 (0 %; 10%; 20 %, dan 30% ), tiap perlakuan diulang sebanyak 4 kali. Data volume produksi air susu yang diperoleh dianalisa dengan ANOVA, dan bila terdapat perbedaan, maka dilanjutkan dengan uji lanjut Duncan. Hasil penelitian menunjukkan pengaruh yang nyata (P0.05) terhadap volume air susu yang dihasilkan antar perlakuan S0, S1, S2 dan S3. Pemberian ekstrak daun katuk 10% (S1) dalam air minum memperlihatkan peningkatan produksi susu dan kualitas susu yang baik dibandingkan dengan pemberian 20% dan 30% dalam air minum. (Giving katuk leaf extract (Sauropus androgynus L. Merr) in drinking water Ettawa crossbreed goats to increase milk volume and milk quality) ABSTRACT. This research was carried out on a group of Ettawa crossbreed goats in Geucee Kayee Jatoe, Banda Aceh. This study used a completely randomized design with 4 (four) treatments of giving katuk leaves extract in drinking water, namely S0, S1, S2 and S3 (0%; 10%; 20%, and 30% of katuk leaf) and each group repeated 4 times. Data on milk production were analyzed by analysis of variant (ANOVA), and if there were differences, then followed by Duncan multiple range test (DMRT). The results showed that there was no significant effect (P 0.05) on the production of milk between treatments S0, S1, S2 and S3. Giving katuk leaf extract up to 10% (S1) in drinking water increase in milk production and quality compared to those giving 20% and 30% of katuk leaf.


2019 ◽  
Vol 2 (1) ◽  
pp. 18
Author(s):  
Yani Yani ◽  
Moh Nuh Ibrahim ◽  
Suwarjoyowirayatno Suwarjoyowirayatno

AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh penambahan daging gurita terhadap nilai organoleptik, proksimat stik gurita. Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari tiga perlakuan yaitu P1 (Daging gurita 35%), P2 (Daging gurita 45%), P3 (Daging gurita 55%). Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf 95%, apabila terdapat beda nyata (p>0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncam Multiple Range Test) pada taraf nyata 95%. Kandungan protein dan lemak diuji menggunakan metode AOAC dan kandungan karbohidrat di uji menggunakan metode nelson-smogiy. Hasil uji kandungan kadar lemak dan karbohidrat tertinggi terdapat pada perlakuan P1 dengan nilai 34,27% dan 7,81% dan Kandungan protein tertinggi pada perlakuan P3 dengan nilai 32,04%. Hasil penelitian ini menunjukan bahwa terdapat pengaruh nyata terhadap kandungan lemak dan karbohidrat serta tidak terdapat pengaruh nyata pada kandungan air dan protein pada stik gurita. Kata kunci: Daging gurita (Octopus cyanea), stik gurita, kandungan proksimat, uji organoleptik  AbstractThis study aims to determine the effect Octopus cyanea addition on organoleptic values and proximate content of octopus sticks. This research method used a completely randomized design (CRD) consisting of three treatments P1 (35% octopus meat), P2 (45% octopus meat), P3 (55% octopus meat). Observation data were analyzed using ANOVA (Analysis of Variance) at the level 95%, if there were significant differences (p>0,05) then further testing was carried out with DMRT (Duncan Multiple Range Test) at 95%. The content of protein and fat was tested by the AOAC method and the carbohydrate content was tested by the nelson-smogiy method. The highest test results for the content of  fat and carbohydrate were found in treatment P1 with a value of 34.27% and 7.81% and the highest protein content found in treatment P3 with a value of 32.04%. The results of this study indicated that there is a real effect on the content of fat and carbohydrates and there is no significant effect on the water content and protein of octopus sticks. Key words: Octopus cyanea, octopus stick, proximate content, organoleptic value


2019 ◽  
Vol 6 (1) ◽  
pp. 49
Author(s):  
Muhammad Arief, Nur Fitriani, Sri Subekti

Abstract Freshwater culture commodity such as sangkuriang catfish (Clarias sp.) has high sufficient demand. One way to fullfil necessity of sangkuriang catfis demand is accelerate the growth. The aim of this research is to determine the present effect of different probiotics on commercial feed towards growth and feed efficiency of sangkuriang catfish. The research using experimental method, using Completely Randomized Design (CRD). Sangkuriang catfish kept in 30 days with four treatments and five replications, that is P1 (control), P2 (5% probiotics A), P3 (5% probiotics B), and P4 (5% probiotics C). The obtained data were processed by Analysis of Variance (ANOVA) and followed by Duncan Multiple Range Test if there was significant data. The result showed that different probiotics giving significant effect to growth rate and feed efficiency (p<0,05). The best sangkuriang catfish (Clarias sp.) growth rate is P2 treatment 2,88% ± 0,38, and the lowest is P1 treatment at 1,73% ± 0,7. The best feed efficiency is P2 treatment at 54,69% ± 9,67 and the lowest is P1 treatment at 30,27% ± 12,65


2019 ◽  
Vol 8 (1) ◽  
pp. 36
Author(s):  
Dewa Ayu Tri Ulandari ◽  
Komang Ayu Nocianitri ◽  
Ni Made Indri Hapsari Arihantana

This study aims to determine the effect of drying temperature on the bioactive component contents of white peony tea and to know the proper drying temperature in white peony tea to produce the highest bioactive component contents and the best sensory characteristics. The experimental design used was Completely Randomized Design with a drying temperature as the treatment consisting of five levels: 60oC, 70oC, 80oC, 90oC, and 100oC. The treatment was repeated 3 times to obtain 15 experiment units. The data obtained were analyzed by variance and if the treatment had an effect on the observed variable then continued with The Duncan Multiple Range Test. The results showed that the 90oC drying temperature treatment produced white peony tea with the highest bioactive component contents and the best sensory characteristics with water content 4,06% (wet basis), yield 52,54%, total phenolic 211,01 mg GAE/g tea, flavonoid 10,90 mg CE/g tea, catechin 3,55 mg/g tea, antioxidant activity 50,20%, brew color yellow and liked, astringent taste and usual, aroma liked and overall acceptance rather liked.


2019 ◽  
Vol 8 (2) ◽  
pp. 150
Author(s):  
Kadek Wiantini ◽  
I Gusti Ayu Ekawati ◽  
Ni Made Yusa

This study aims to determine the effect of cowpea sprout paste and seaweed paste to the characteristics of analogue cowpea sausage, and to know the right ratio of cowpea sprout paste and seaweed paste on the characteristics of analogue cowpea sausage. The experimental design used was completely randomized design with the treatment ratio of cowpea sprout paste and seaweed paste, which consists of 5 levels such as: 90% cowpea sprout paste : 10% seaweed paste, 80% cowpea sprout paste : 20% seaweed paste, 70% cowpea sprout paste : 30% seaweed paste, 60% cowpea sprout paste : 40% seaweed paste, and 50% cowpea sprout paste: 50% seaweed paste. The treatment was repeated 3 times to obtain 15 units of experiments. The data were analyzed by variance analysis and if the treatment had a significant effect then continued with DMRT (Duncan Multiple Range Test). The results showed that cowpea sprout paste and seaweed paste ratio had a significant effect on water content, protein content, fat content, carbohydrate content, coarse fiber content, elasticity, taste (hedonic), texture (hedonic and scoring) and overall acceptance (hedonic). Comparison of 50% cowpea sprout paste and 50% seaweed paste had the best characteristics analogue cowpea sausage, with 69,88% water content, 2,16% ash content, 3,77% protein content, 2,92% fat content, 21,27% carbohydrate content, 1,50% coarse fiber content, elasticity 6,65 N, color and aroma rather liked, texture chewy and liked, taste and overall acceptance liked.


2017 ◽  
Vol 17 (2) ◽  
pp. 95-103 ◽  
Author(s):  
Andi Mulyadi ◽  
Edjeng Suprijatna ◽  
Umiyati Atmomarsono

ABSTRAK. Limbah udang merupakan limbah hasil industri pengupasan udang yang terdiri dari kepala dan kulit yang dapat dimanfaatkan sebagai pakan alternatif dalam ransum puyuh. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian Tepung Limbah Udang Fermentasi (TLUF) dalam ransum puyuh untuk meningkatkan kecernaan. Materi penelitian menggunakan 250 ekor puyuh umur 6 minggu dengan bobot awal rata-rata 140,95±9,58 (CV=6,80). Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) terdiri dari 5 perlakuan dan 5 ulangan, setiap unit percobaan terdiri dari 10 ekor puyuh. Pakan perlakuan yang digunakan adalah 0%, 5%, 7,5% dan 10% tepung limbah udang fermentasi, serta 7,5% tepung limbah udang tidak fermentasi. Data dianalisis menggunakan analisis ragam (ANOVA) dengan uji F pada taraf 5% dan apabila terdapat pengaruh perlakuan dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa pengaruh pemberian tepung limbah udang fermentasi dalam ransum puyuh berpengaruh nyata (P0,05) meningkatkan skor warna kuning telur tetapi tidak berpengaruh nyata (P0,05) terhadap indeks kuning telur, indeks putih telur dan nilai Haugh Unit. Kesimpulan dari penelitian ini adalah pemberian tepung limbah udang fermentasi 10% mampu meningkatkan skor warna kuning telur. (The effect of supplementation of quails diet with fermented shrimp waste meal on eggs quality)ABSTRACT. Shrimp waste is a waste product of shrimp peeling industry which consists of head and skin. These waste product, can be used as alternative feed stuffs for quails diet. The research was conducted to study the effect of supplemenetation of the diet with fermented shrimp waste meal on quails egg quality. The experimental animals used were 250 quails of 6 weeks old with the initial weight of 140,95±9,58 (CV=6,80). Completely Randomized Design was used with 5 treatments and 5 replicate cages of 10 quails each cage. The treatments were fermented shrimp waste meal with the levels of 0%, 5%, 7,5% and 10%, and 7,5% unfermented shrimp waste meal. Data were analyzed using analysis of variance and any differences among treatment means found in the analysis of variance were further tested by Duncan Multiple Range Test. The results showed that the fermented shrimp waste meal had significant affect on yolk colour (P0,05), but produced no significant effet (P0,05) on yolk index, albumen index and haugh unit. In conclusion 10% fermented shrimp waste meal can increased yolk colour.


Agrivet ◽  
2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Pauliz Dwi Hastuti ◽  
Sri Manu Rohmiyati ◽  
Ashabul Kahfi

The aim of this research was to determine the effective volume of water in several types of soil for the growth of Mucuna bracteata. This research was conducted at the Education and Research Garden (KP-2) of the Stiper Agricultural Institute located in Maguwoharjo, Sleman, DIY. This study uses factorial experiments arranged in Completely Randomized Design (CRD) consisting of two factors and five replications. The first factor was the volume of water spray/day/plant consisting of four volume levels, namely: 50 ml, 100 ml, 150 ml and 200 ml. The second factor was the type of soil consisting of three types of soil, namely : regosol, latosol and grumusol. The results of the research data were analyzed using variance (Analysis of variance) at a real level of 5%. Data that is significantly different is continued with Duncan's multiple distance test or DMRT (Duncan multiple range test) at a real level of 5%. The results showed that there was no good combination of the volume of water and soil type on the growth of Mucuna bracteata. The volume of 50 ml / plant water was sufficient to produce good Mucuna bracteata plant growth. Mucuna bracteata plants can grow in regosol, latosol, and grumusol soil types.Keywords: volume, water, soil type, Mucuna bracteata


2021 ◽  
Vol 3 (2) ◽  
pp. 52
Author(s):  
Catur Suci Purwati ◽  
Engkus Ainul Yakin ◽  
Febrian Nisa Ramadhani

This study aimed to determine the performance of laying hen eggs on the addition of sprouts at Wakhid Farm, Magetan Regency. This research was conducted at Wakhid Farm, Magetan Regency. Sprouts were soaked for 1 day before being given to the treatment feed. This study used 4 types of treatment and each treatment consisted of 4 replications. P0 is control (50% corn + 15% bran + 35 concentrate), P1 is 50% corn + 15% bran + 35% concentrate + 5% sprouts, P2 is 50% corn + 15% bran + 35% concentrate + 10% sprouts and P3 which is 50% corn + 15% bran + 35% concentrate + 15% sprouts. Observation parameters include feed consumption, hen day production, egg weight and egg mass. Parameters observed in the study included feed consumption, egg production, hen day production, egg weight and egg mass. The research method used RAL (Completely Randomized Design) unidirectional pattern. The data obtained were then analyzed using ANOVA (Analysis of variance). If there is a significant treatment effect, then proceed with the DMRT Test (Duncan Multiple Range Test) to determine the differences between treatments. The data obtained showed that the performance of different eggs for each treatment sample had a significant effect on feed consumption, hen day production, egg weight and egg mass. The results showed that feed consumption was between 118.7 – 118.5 grams/day/head, hen day production was between 79.46 – 90.17%, egg weight was between 57.08 – 60.47 grams and egg mass was 43.94 – 56.55 grams. The conclusion was that the addition of sprouts did not affect feed consumption, hen day production and egg mass and had a significant effect on egg weight.. 


2014 ◽  
Vol 16 (1) ◽  
pp. 7
Author(s):  
Reni Surya Dewi ◽  
Samanhudi Samanhudi ◽  
Dwi Hardjoko

<p>Changed over of the functions of agricultural land into industrial areas causing the narrowness of potential agricultural land can be used for cultivation. Hydroponics is able to overcome these problems, one of them by utilizing arenga wood fiber as a substrate for planting. One type of vegetable which is easily cultivated mustard plants are especially pakchoi (Brassica rapa L. ssp. chinensis). In addition to the timing of harvest is short, the plant also has a high economic value. The purpose of this research is to know the composition of the mixture of arenga wood fiber for proper growth of the pakchoi. This research was carried out in July to September 2013 in Screen house B Sebelas Maret University Surakarta Faculty of agriculture. Research compiled based on Completely Randomized Design (CRD) with two factor, each consisting of three degrees so obtained nine ranks and one control which use husk charcoal and the treatment is replication four times. The Data obtained were analyzed with F-test standard of 5%. If there is a real significant on the treatment of variables measured then continued with average comparison test using Duncan Multiple Range Test (DMRT) at the 95% confidence level. The research shows that the arenga wood fiber can be used as a medium in hydroponic substrates for pakchoi and combinations are optimal for the growth of pakchoi is arenga wood fiber soaked water 6 days mix sand volcano not washed.</p>


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