Tomato (Lycopersicon esculentum Mill) seedlings were grown with air temperature of 28°C light/12°C dark (12/12 hours), and either a constant, 20°C, root-zone temperature (RZT), or in-phase with air temperature, 28°C in the light and 12°C in the dark, or out-of-phase, 12°C in the light and 28°C in the dark. These treatments were applied from 17 to 25 days after germination, with 200 m \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{-}^{3}\) \end{document} in flowing nutrient solution. The relative growth rate of leaves was the greatest with constant RZT, 0.33/d, and least with out-of-phase RZT, 0.29/d. The concentration of free amino acid and protein in leaves was least for out-of-phase RZT. The \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{-}^{3}\) \end{document} concentration in leaves was the highest in the dark, intermediate in the middle of the light period, and the lowest at the end of the light period. In roots, \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{-}^{3}\) \end{document} concentration showed a similar trend. This variation was greatest when RZT was varied out of phase, and least with constant RZT. At the end of the light period, \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{-}^{3}\) \end{document} concentration in roots was 246, 180, and 162 μmol·g–1 dry weight for constant, in phase, and out of phase RZT, respectively. In the light, leaves of seedlings grown with out-of-phase RZT had 5 mmol·g–1 \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{-}^{3}\) \end{document}, compared to 16 mmol·g–1 with in-phase RZT Availability of \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{-}^{3}\) \end{document} in the light may be the factor limiting plant growth with out-of-phase RZT. This research was supported in part by grant number 93-37100-9101 from the National Research Initiative Competitive Grants Program/USDA.