scholarly journals Glutathione system in the blood serum of rats in the dynamics of full-thickness wounds and with the influence of new pharmacological composition containing melanine

2017 ◽  
Vol 22 (1) ◽  
pp. 5-8
Author(s):  
O. Taburets ◽  
K. Dvorshenko ◽  
M. Tymoshenko ◽  
V. Vereschaka ◽  
T. Beregova ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Glutathione Transferase ◽  
Reductase Activity ◽  
Transferase Activity ◽  
Full Thickness ◽  
Glutathione System ◽  
Glutathione Reductase Activity ◽  
Activity Of Enzymes ◽  
Full Thickness Wound

The activity of enzymes of the glutathione link of the antioxidant system in rats with experimental full-thickness wound has been studied. It is established that in the serum of rats under the experimental model of the cut wound the level of recovered glutathione and glutathione reductase activity decreased, glutathione peroxidase and glutathione transferase activity increased. All investigated parameters normalized in dynamics after treatment with melanin.

scholarly journals ACTION OF CHONDROITIN SULFATE ON THE GLUTATHIONE SYSTEM IN BLOOD SERUM AT СARRAGEENAN-INDUCED ACUTE INFLAMMATION

2017 ◽  
Vol 73 (1) ◽  
pp. 60-62
Author(s):  
K. Dvorshchenko ◽  
M. Ashpin ◽  
E. Torgalo ◽  
M. Tymoshenko ◽  
L. Ostapchenko
Keyword(s):  
Blood Serum ◽  
Chondroitin Sulfate ◽  
Acute Inflammation ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Transferase Activity ◽  
Oxidized Glutathione ◽  
Glutathione System ◽  
Experimental Conditions ◽  
Rat Paw

Increase of content of oxidized glutathione (GSSG) is fixed in blood serum at carrageenan-induced rat paw inflammation, as well as increase of the glutathione transferase activity. Upon the same experimental conditions, the level of reduced glutathione (GSH) and activity of glutathione peroxidase and glutathione reductase were decreased. All above mentioned indices was closer to control values in animals treated simultaneously with сarrageenan and drug on the basis of chondroitin sulfate.

scholarly journals Glutathione system in rat blood serum under the conditions of carrageenan-induced joint inflammation and chondroitin sulfate prophylactic injection

2021 ◽  
Vol 85 (2) ◽  
pp. 32-37
Author(s):  
K. Dvorshchenko ◽  
O. Korotkiy ◽  
D. Grebinyk ◽  
Ye. Torgalo
Keyword(s):  
Glutathione Peroxidase ◽  
Chondroitin Sulfate ◽  
Peroxidase Activity ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Joint Inflammation ◽  
Transferase Activity ◽  
Glutathione System ◽  
Nacl Solution ◽  
Glutathione Peroxidase Activity

The goal of this work was the research of chondroitin sulfate prophylactic action on the glutathione system state in rat blood serum during acute joint inflammation. The research was performed on white non-linear pubertal rat males weighting 160-240 grams in compliance to all general ethical principles of animal research. All animals were divided to four experimental groups. The first group – the control: the animals were injected with 0,1 ml of 0,9 % NaCl solution in a right hind leg. The second group – rats were subjected to daily intramuscular injections of 3 mg/kg of chondroitin sulfate in the theurapeutic dose during 28 days. The third group – the animals were subjected to daily doses of 0,1 ml of 0,9 % NaCl solution injected in right hind extremities and starting from 29th day the acute joint inflammation was modelled (the animals were subjected to 0,1 ml of 1% of сarrageenan intramuscular injection in right hind extremities. The fourth group – rats were receiving intramuscular injections of therapeutic dose of 3 mg/kg of chondroitin sulfate for 28 days, and after that the acute joint inflammation was modelled starting from 29th day. 40 animals in all were taking part in the experimental research. The glutathione peroxidase activity was assessed judging from the decrease in GSH amount in the reaction with Ellman reagent. The glutathione peroxidase activity was estimated by the decrease in probe optical density owing to NADPH oxidation. Glutathione transferase activity was estimated by the speed of the conjugate formation between GSH and 1-chloro-2,4-dinitrobemzene. The amount of reduced glutathione was estimated spectrophotometrically using orthophthalic aldehyde. It was established that during the carrageenan-induced inflammation the glutathione peroxidase, glutathione reductase and glutathione reductase activity were reduced, a well as the amount of reduced glutathione, whereas the glutathione transferase activity was increased in comparison to the control. It was shown that under the prophylactic injection of the chondroitin sulfate based preparate to animals with acute joint inflammation the aforementioned parameters were partially stabilized.

scholarly journals АНТИОКСИДАНТНИЙ ЗАХИСТ ТА ПЕРОКИСНЕ ОКИСНЕННЯ ЛІПІДІВ ОРГАНІЗМУ ВИСОКОТІЛЬНИХ КОРІВ ПРИ ПРОФІЛАКТИЦІ САЛЬМОНЕЛЬОЗУ ЗА ВПЛИВУ НАНОПРЕПАРАТУ ГЕРМАКАПУ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 112-116
Author(s):  
P.Y. Lavriv
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Antioxidant Defense ◽  
Reductase Activity ◽  
Lipid Body ◽  
Antioxidant Defense System ◽  
Glutathione System ◽  
Defense System ◽  
Glutathione Reductase Activity ◽  
Radical Processes

The article  deals with the results of experimental research data of relationship between the antioxidant defense system and lipid body peroxidation of high calves cows  under  the influence of nanpreparation Germakap conducted  with simultaneous vaccination with inactivated formol vaccination with repeated  in two weeks later  at the same doses as their  stability and immunity to Salmonella. It was found the likely increase in activity of glutathione peroxidase and glutathione reductase and at the same time, reduction of malon dialdehyde and hydroperoxides lipids and superoxidimutase. These changes in animals  body occur due to complex components adaptive nan preparation Germakap that lead to the normalization of metabolic and free radical processes in animals.  However, the increase in the catalytic activity of glutathione peroxidase and glutathione reductase activity in plasma of high calves cows  from research group can be explained by increasing intensity of synthesis in which these enzymes by introducing nan preparation Germakap closely associated with the regeneration of glutathione in the cell, and also the activity of glutathione peroxidase.  Through interaction with restoration of glutathione and glutathione peroxidase, glutathione reductase it was formed glutathione system that protects cells from stress peroxidation

scholarly journals Glutathione system state in a hemolysate of erythrocytes among the patients with chronic cerebral ischemia

2016 ◽  
pp. 37-43
Author(s):  
A.V. Demchenko
Keyword(s):  
Cerebral Ischemia ◽  
Glutathione Peroxidase ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Healthy People ◽  
System Level ◽  
Transferase Activity ◽  
Glutathione System ◽  
Chronic Cerebral Ischemia ◽  
Instrumental Methods

The purpose of the work is to study a glutathione system (level of reduced glutathione and activity of glutathione-dependent enzymes) in a hemolysate of erythrocytes among the patients with chronic cerebral ischemia (CCI). It was examined 287 patients with CCI and 50 healthy people without any signs of cerebrovascular disease using clinical and neuropsychological, laboratory and instrumental methods of investigation. It was established a significant decrease of the reduced glutathione concentration, the decrease of glutathione reductase and glutathione peroxidase and the increase of glutathione transferase activity in a hemolysate of erythrocytes among the patients with chronic cerebral ischemia in comparison with these rates among healthy people. It was established a negative correlated connection between the level of the reduced glutathione, the age of the patients (r=-0,34, р<0,0001), the stage of discirculatory encephalopathy (DE) (r=-0,50, р<0,0001), intima media complex thickness of carotid arteries (r=-0,29, р=0,009), the level of homocysteine (r=-0,81, р<0,0001) and also the level of glutathione peroxidase, the stage of DE (r=-0,39, р<0,0001) and homocysteine level (r=-0,60, р<0,0001).

scholarly journals Glutathione-dependent system in the blood of gastric cancer patients with various tumor histotypes and prevalence of the disease

2021 ◽  
Vol 8 (4) ◽  
pp. 12-22
Author(s):  
I. A. Goroshinskaya ◽  
E. I. Surikova ◽  
E. M. Frantsiyants ◽  
L. A. Nemashkalova ◽  
P. S. Kachesova ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Stage Iv ◽  
Signet Ring Cell ◽  
Transferase Activity ◽  
Low Grade ◽  
Glutathione System ◽  
Maximum Increase

Purpose of the study. Exploring the erythrocyte glutathione system functioning in patients with gastric cancer in a comparative aspect, depending on tumor histotype and disease prevalence.Patients and methods. The study included 89 patients with gastric cancer, divided into 6 groups depending on the histotype of the tumor. Separately, the results of the study were analyzed in patients with T4 status according to TNM and in patients at stage 4. The content of reduced glutathione and the activity of glutathione-dependent enzymes in the erythrocytes of the patients' blood were studied by conventional spectrophotometric methods. Statistical processing of the results was carried out using the Statistika 6.0 software package according to the Student's t-test and the nonparametric Wilcoxon-Mann-Whitney test for two independent samples.Results. An increase in the content of glutathione was revealed in patients with gastric cancer compared with the group without oncopathology. The maximum increase was observed in patients with low-grade adenocarcinoma - by 42.5 %, while in signet ring cell carcinoma (SRCC) there was only a tendency to increase by 17.8 %. Glutathione reductase activity was decreased in adenocarcinoma by 23.4-26.2 % and did not change in SRCC. The activity of the antioxidant enzymes glutathione peroxidase and glutathione transferase was increased in all groups, and especially in SRCC - by 76 % and 23-29 %, respectively. In patients with the T4 status and at stage IV of the process, a lower activity of the studied glutathione-dependent enzymes was revealed in comparison with all other groups of patients.Conclusions. The data obtained indicate a greater functional potential of the glutathione system in SRCC. A significant increase in glutathione transferase activity at a sufficiently high level of reduced glutathione can contribute to the development of treatment resistance in SRCC patients.

scholarly journals Correlation of glutathione reductase activity distribution in the blood serum and tissues of white unborn rats

2020 ◽  
Vol 17 ◽  
pp. 00195
Author(s):  
Pavel Boriskin ◽  
Olga Gulenko ◽  
Anatoly Devyatkin ◽  
Rufia Karimova ◽  
Victor Leonov ◽  
...  
Keyword(s):  
Blood Serum ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Active Oxygen Species ◽  
Free Radical Oxidation ◽  
Rat Tissues ◽  
Activity Distribution ◽  
Glutathione Reductase Activity ◽  
Nonparametric Correlation ◽  
The Relationship

It is known that the majority of pathological processes take place against the background of formation of active oxygen species and intensification of free radical oxidation of bio-substrates. In response to this, the antioxidant system of the cell is activated, and the glutathione system is an important link in this system. The latter can take part in the maintenance of the optimal state of biomembranes, in the processes of detoxification, antioxidant protection, etc. The biological role of glutathione reductase is to maintain high intracellular concentration of reduced glutathione. The aim of our study was to study the relationship between the distribution of glutathione reductase activity in blood serum and rat tissues. In order to achieve the goal of the study the following tasks were solved: the activity of glutathione reductase in blood serum and tissues of liver, brain, heart, as well as in skeletal muscle tissues of rats was determined; the interrelation of the activity distribution of glutathione reductase in blood serum and tissues of rats was revealed. The article presents the results of nonparametric correlation analysis to assess the relationship between the distribution of glutathione reductase activity in blood serum and tissues of small experimental animals.

Glutathione system in the blood of gastric cancer patients with various tumor histotypes and prevalence of the disease.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16534-e16534
Author(s):  
Irina A. Goroshinskaya ◽  
Ekaterina I. Surikova ◽  
Elena M. Frantsiyants ◽  
Ludmila A. Nemashkalova ◽  
Polina S. Kachesova ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Cell Cancer ◽  
Low Grade ◽  
Glutathione System ◽  
Incidence And Mortality

e16534 Background: The role of oxidative stress in tumor growth and the multifaceted functional significance of glutathione have led to an interest in studying the components of the glutathione-dependent system in gastric cancer (GC), which occupies a leading position in cancer incidence and mortality. The aim of this work was to study the functioning of the glutathione system in the red blood cells of patients with gastric cancer in a comparative aspect depending on the histotype of the tumor and the prevalence of the disease. Methods: The study included 89 patients with GC divided into 6 groups depending on the histotype of the tumor. Separately, the results of the study were analyzed in patients with T4 status according to TNM and in patients with stage IV cancer. The content of reduced glutathione and the activity of glutathione-dependent enzymes were studied by conventional spectrophotometric methods in the red blood cells of patients. Statistical processing of the results was performed using the Statistika 6.0 program with Student’s t-test for two independent groups. Results: An increase in the content of glutathione in patients with GC compared with the group without oncopathology was revealed. The maximum increase was observed in patients with low-grade adenocarcinoma - by 42.5%, while in signet ring cell cancer (SRCC) there was only a tendency to increase by 17.8%. Glutathione reductase activity was reduced in adenocarcinoma by 23.4-26.2% and did not change in SRCC. The activity of the antioxidant enzymes glutathione peroxidase and glutathione transferase was increased in all groups and especially in SRCC - by 76% and 23-29%, respectively. In patients with T4 status and at stage 4 of the process, lower activity of the studied glutathione-dependent enzymes was revealed compared with all other groups of patients. Conclusions: The data indicate a greater functional potential of the glutathione system in SRCC. A significant increase in the activity of glutathione transferase with a sufficiently high level of reduced glutathione contributes to the development of treatment resistance in patients with SRCC.

scholarly journals Activity of glutathione-dependent enzymes in spermatozoa in patients with pathospermia

2017 ◽  
Author(s):  
O. K. Onufrovych ◽  
R. V. Fafula ◽  
Io. A. Nakonechnyi ◽  
D. Z. Vorobets ◽  
U. P. Iefremova ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Antioxidant System ◽  
Reductase Activity ◽  
Sperm Cells ◽  
Compensatory Mechanisms ◽  
Glutathione Antioxidant System ◽  
Healthy Men ◽  
Infertile Men ◽  
Glutathione Reductase Activity

The results of glutathione peroxidase and glutathione reductase activity in spermatozoa of patients with different forms pathospermia are presented in the paper. It was shown that glutathione peroxidase and glutathione reductase activity in sperm cells of patients is reduced in comparison with healthy men with preserved fertility. However, the most expressed changes in the activity of glutathione peroxidase and glutathione reductase are in spermatozoa of infertile men with associated forms patospermia and leucospermia. These changes indicate exhaustion of compensatory mechanisms of glutathione antioxidant system in the sperm cells of infertile men with pahospermia.

Analysis of Glutathione-Dependent Enzyme Activities in two Different Rat Hepatomas and in Normal Liver in Relation to their Role in Resistance to Oxidative Stress

1988 ◽  
Vol 74 (6) ◽  
pp. 617-621 ◽  
Author(s):  
Maria Armida Rossi ◽  
Mario Umberto Dianzani
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Antioxidant Properties ◽  
Liver Microsomes ◽  
Normal Liver ◽  
Lipid Hydroperoxides ◽  
Glutathione S Transferases ◽  
Glutathione Reductase Activity

The importance of some glutathione metabolic pathways was examined in two highly dedifferentiated hepatomas, Yoshida AH-130 and Morris 3924 A hepatomas, and in normal liver in relation to their role against oxidative stress. The cytosol prepared from Yoshida hepatoma cells decreased the peroxidation rate in normal liver microsomes and mitochondria, but this antioxidant property was not displayed by Morris hepatoma. Glutathione peroxidase and glutathione-S-transferases activities were extremely low in both hepatomas; glutathione reductase activity values were about half the normal liver values. The large decrease in glutathione peroxidase and glutathione-S-transferases suggests that in these two tumors only small amounts of GSH can be used in reduction or conjugation reactions, such as the reduction of hydrogen peroxide and lipid hydroperoxides or the conjugation of GSH with the end products of lipoperoxidation, aldehydes or ketones. The hypothesis of a more efficient GSSG reduction in hepatomas, due to the low glutathione peroxidase/glutathione reductase activity ratio, is also discussed. The described changes in glutathione related enzymes do not seem to have any correlation with the protective effect against the lipoperoxidative processes displayed by some tumors since these enzymatic activities were similar in both hepatomas whereas only Yoshida hepatoma showed antioxidant properties.

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