scholarly journals A New Oxidative Derivatization Method For The Indirect Spectrofluorimetric Determination Of Prochlorperazine Maleate In Pharmaceutical Preparations

2019 ◽  
Vol 14 (3) ◽  
pp. 140-145
Author(s):  
M.Ye. Blazheyevskiy ◽  
Yu.V. Skrypynets ◽  
A.V. Yegorova ◽  
V.P. Antonovich
Keyword(s):  
Sulfuric Acid ◽  
Acid Solution ◽  
Quantitative Determination ◽  
Calibration Curve ◽  
Pharmaceutical Preparations ◽  
Limit Of Quantification ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination ◽  
Derivatizing Agent

A new oxidative derivatization method for the indirect spectrofluorimetric determination of Prochlorperazine maleate has been presented. Potassium hydrogenperoxomonosulphate is proposed as a derivatizing agent for Prochlorperazine, yielding the strongly fluorescent sulfoxide. This reaction product was successfully employed for the spectrofluorimetric determination of the Prochlorperazine maleate. A highly sensitive, simple and rapid method has been developed for determining prochlorperazine maleate in tablets by fluorescence of its oxidation product with Oxone solution in 0.01 M sulfuric acid solution (λex = 340 nm; λem = 380 nm). The calibration curve is linear in its concentration range of 0.8–10.0 µg/ml. Limit of quantification (LOQ = 10S) is 0.8 µg/ml. The possibility of quantitative determination of Prochlorperazine maleate in Vertinex® tablets 5 mg has been shown, RSD <2.3% (δ <RSD).

scholarly journals The Oxidative Derivatization Method For The Spectrofluorimetric Determination Of Periciazine In Pharmaceutical Preparations

2020 ◽  
Vol 15 (1) ◽  
pp. 21-26
Author(s):  
M.Ye. Blazheyevskiy ◽  
Yu.V. Scrypynets ◽  
A.V. Yegorova ◽  
V.P. Antonovich
Keyword(s):  
Hydrochloric Acid ◽  
Acid Solution ◽  
Quantitative Determination ◽  
Pharmaceutical Preparations ◽  
Oral Solution ◽  
Calibration Plot ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination ◽  
Derivatizing Agent

The oxidative derivatization method for the indirect spectrofluorimetric determination of Periciazine has been presented. Potassium hydrogenperoxymonosulfate (Oxone ®) is proposed as a derivatizing agent for Periciazine, yielding the strongly fluorescent Periciazine sulfoxide. A highly sensitive, simple and rapid method for determination of the Periciazine by fluorescence of its oxidation product with Oxone ® solution in 0.02 M hydrochloric acid solution (λex = 364 nm; λem = 444 nm) has been developed. The calibration plot is linear in concentration range of 0.05 – 4.00 µg mL -1 . LOQ (10S) is 0.05 µg mL -1 . The possibility of quantitative determination of Periciazine in pharmaceutical preparations (Neuleptil ®, 10 mg capsules and Neuleptil ®, a 30 mL 4 % oral (solution) drops) has been shown RSD < 2.2 % (δ < RSD).

scholarly journals Difference spectrophotometric method for the determination of Fluphenazine hydrochloride in tablets using peroxomonosulfate

2021 ◽  
Vol 9 (2) ◽  
pp. 64-71
Author(s):  
Mykola Blazheyevskiy ◽  
◽  
Valeriy Moroz ◽  
Olena Mozgova ◽  
◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Present Method ◽  
Molar Absorptivity ◽  
Limit Of Quantification ◽  
Derivatizing Agent ◽  
Sensitive Method

The oxidative derivatization method using potassium hydrogenperoxomonosulfate for the indirect spectrophotometric determination of Fluphenazine hydrochloride is presented. Potassium hydrogenperoxomonosulfate is introduced as a derivatizing agent for Fluphenazine hydrochloride, yielding the sulfoxide. This reaction product was successfully used for the spectrophotometric determination of the Fluphenazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Fluphenazine hydrochloride in the concentration range of 0.2-30 µg mL-1. The molar absorptivity at 349 nm is 5.6×103 (dm3cm-1mol-1). The limit of quantification, LOQ (10S) is 0.24 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Fluphenazine hydrochloride in tablets 5.0 mg was demonstrated. The present method is precise, accurate and excipients did not interfere. RSD for Fluphenazine Hydrochloride 5.0 mg tablets was 1.37 %.

scholarly journals Quantitative determination of Levomepromazine in pharmaceuticals by spectrophotometric method as its sulfoxide

2020 ◽  
Vol 8 (1) ◽  
pp. 117-124
Author(s):  
Olena Mozgova ◽  
Mykola Blazheyevskiy
Keyword(s):  
Sodium Chloride ◽  
Citric Acid ◽  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Present Method ◽  
Limit Of Quantification ◽  
Derivatizing Agent ◽  
Sensitive Method

The oxidative derivatization method using Diperoxyazelaic acid for the indirect spectrophotometric determination of Levomepromazine hydrochloride is presented. Diperoxyazelaic acid is introduced as a derivatizing agent for Levomepromazine, yielding the sulfoxides. This reaction product was successfully used for the spectrophotometric determination of the Levomepromazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Levomepromazine hydrochloride in the concentration range of 3-150 µg/mL. The limit of quantification, LOQ (10S) is 2.85 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Levomepromazine in Tisercin Solution for Injection 25mg/mL was demonstrated. The present method is precise, accurate and other excipients: anhydrous citric acid, monothioglycerol, sodium chloride did not interfere. RSD = 1.24 % (δ = –0.02 %).

Toxicologic Studies on Hydrocarbons. VI. A Colorimetric Method for the Determination of Kerosine in Blood

1960 ◽  
Vol 6 (4) ◽  
pp. 327-331 ◽  
Author(s):  
Horace W Gerarde ◽  
Paul Skiba
Keyword(s):  
Sulfuric Acid ◽  
Carbon Tetrachloride ◽  
Quantitative Determination ◽  
Calibration Curve ◽  
Colorimetric Method ◽  
Acid Reagent

Abstract A photoelectric colorimetric method is described for the quantitative determination of kerosine in blood. The procedure involves hemolysis of 5 ml. of the sample followed by extraction of the kerosine with carbon tetrachloride. The extract is reacted with a formaldehyde- sulfuric acid reagent to produce a characteristic color. The intensity of this color is measured photometrically, and the concentration of kerosine is determined by reference to a previously prepared calibration curve. Concentrations as low as 10 ppm can be conveniently determined.

scholarly journals Highly Sensitive Spectrofluorimetric Determination of Ephedrine Hydrochloride in Pharmaceutical Preparations

2006 ◽  
Vol 89 (5) ◽  
pp. 1263-1267 ◽  
Author(s):  
Sevgi Tatar Ulu
Keyword(s):  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Official Method ◽  
Good Precision ◽  
Experimental Conditions ◽  
Ephedrine Hydrochloride ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Spectrofluorimetric Determination

Abstract A sensitive and specific spectrofluorimetry method was developed and validated for the quantification of ephedrine (EP) in pharmaceutical preparations. The method is based on the fluorescent enhancing reaction of EP with 7-chloro-4-nitrobenzofurazan (NBD-CI; derivatization reagent), in borate buffer of pH 9 to yield a yellow, fluorescent product. Under these experimental conditions, the derivatized product of EP had excitation and emission wavelength maxima at 458 and 516 nm, respectively. The linear range of this method was 202500 ng/mL. The detection limit was 7.3 ng/mL EP. Intra- and interday precisions of the assay at 3 concentrations within this range were 0.0371.77%. The low relative standard deviation values indicate good precision, and high recovery values indicate excellent accuracy of the method. The proposed method was applied to the determination of the examined drugs in pharmaceutical formulations, and the results indicate that the method is equally as accurate, precise, and reproducible as the official method.

scholarly journals A new oxidative derivatization method for spectrophotometric determination of Periciazine in pharmaceutical preparations

2019 ◽  
Vol 7 (2) ◽  
pp. 52-60 ◽  
Author(s):  
Mykola Blazheyevskiy ◽  
Valeriy Moroz
Keyword(s):  
Spectrophotometric Determination ◽  
Pharmaceutical Preparations ◽  
Drug Dosage ◽  
Official Method ◽  
Limit Of Quantification ◽  
Selective Method ◽  
Derivatizing Agent ◽  
Phenothiazine Drug ◽  
Good Agreement

A new the oxidative derivatization method by means of peroxoacid for the indirect spectrophotometric determination of Periciazine is presented. A potassium hydrogenperoxymonosulfateas a derivatizing agent for Periciazine, yielding the absorbative Periciazine sulfoxide at λmaх=362 nm is proposed. This reaction product was successfully employed for spectrophotometric determination of the Periciazine. The UV spectrophotometric determination of the Periciazine as its sulfoxide proved to be the more simple and selective method. Limit of quantification (LOQ=10S) is 2.8 µg·mL-1. The common excipients employed do not interfere in the determination of phenothiazine drug. Results of analysis of the drug dosage forms by the proposed method are in good agreement with those of the official method. RSD=1.76 % (δ <RSD).

The Application of TLC and Densitometry for Quantitative Determination of Meloxicam in Tablets

2019 ◽  
Vol 15 (7) ◽  
pp. 785-794
Author(s):  
Wioletta Parys ◽  
Katarzyna Bober ◽  
Alina Pyka-Pająk ◽  
Małgorzata Dołowy
Keyword(s):  
Quantitative Determination ◽  
Silica Gel ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Aluminium Oxide ◽  
Complete Separation ◽  
Limit Of Quantification ◽  
Antipyretic Activity ◽  
Anti Inflammatory

Background: Meloxicam is as a non-steroidal anti-inflammatory drug that indicates a strong anti-inflammatory, analgesic and antipyretic activity. It is used in the treatment of osteoarthritis arthritis, osteoarthritis and rheumatoid arthritis in the form of various pharmaceutical preparations. Objective: The aim of the work was an elaboration of chromatographic conditions enabling the complete separation of impurities A and B from meloxicam and also its quantitative determination in tablets with use of TLC combined with densitometry as well as the comparison of the method proposed with that described in the literature by Starek and Krzek. Methods: The mixture of ethyl acetate: toluene: n-butylamine (2:2:1, v/v/v) was used as a mobile phase. Determination of meloxicam was performed on silica gel and aluminium oxide plates. Chromatographic conditions presented in this work are better than those described by Starek and Krzek. Results: Linearity of the method for both types of plates was in the range from 1.0 to 5.0 µg/spot. Limit of quantification for silica gel plates was 0.18 µg/spot, while for aluminium oxide plates it was 0.26 µg/spot. Limit of detection has been also specified, 0.06 μg/spot for silica gel plates and 0.08 μg/spot for aluminium oxide plates. The average amount of meloxicam in tablets obtained on silica gel plates was 100.4%, and on the aluminium oxide plates it was 100.3%. Conclusion: The developed method of determination of meloxicam using thin layer chromatography combined with densitometry turned out to be accurate, precise and specific. It can be successfully applied in quality control of meloxicam.

scholarly journals The first result in the determination of the percentage concentration of sulfuric acid solution based on the gamma transmission technique with an energy of 662 ke

2021 ◽  
Vol 5 (2) ◽  
pp. first
Author(s):  
Chuong Dinh Huynh ◽  
Sang Thanh Truong ◽  
Trang Thi Ngoc Le ◽  
Linh Thi Truc Nguyen ◽  
Tam Duc Hoang
Keyword(s):  
Sulfuric Acid ◽  
Acid Solution ◽  
Sulfuric Acid Solution ◽  
Calibration Curve ◽  
Rapid Determination ◽  
Simulated Data ◽  
Transmission Technique ◽  
Different Temperatures ◽  
Percentage Concentration

In this study, we propose an approach using the gamma transmission technique based on the combination of the experimental and simulated data for determining the percentage concentration of the acid solution. The experiment consists of a collimated 137Cs radioactive source emitting a narrow gamma beam of 662 keV and a NaI(Tl) detector. Monte Carlo simulation data is used to construct the calibration curve of lnR versus the percentage concentration of acid solution at different temperatures (R is the ratio of the area under a transmission peak for an acid solution relative to that for water). The percentage concentration of the acid solution is determined based on the calibration curve and the experimental ratio R. The obtained results showed that this approach was feasible in which the percent concentration of 7 in total 8 samples of the sulfuric acid solution are less than 5% in comparison with one of the reference samples. There is only one sample with a relative deviation of 7.6%. These preliminary results suggest that it is possible to develop nondestructive testing for the rapid determination of the percentage concentration of acid solutions based on the gamma transmission technique.

Simultaneous Determination of Methocarbamol and Paracetamol in the Presence of Three Related Substances by Ultra Performance Liquid Chromatography

2019 ◽  
Vol 15 (5) ◽  
pp. 505-510
Author(s):  
Yanjuan Zheng ◽  
Qiushi Peng ◽  
Rui Dong ◽  
Tingyu Chen ◽  
Yi Bao ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pharmaceutical Preparations ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Related Substances ◽  
Bulk Powder ◽  
Highly Sensitive ◽  
Optimal Protocol ◽  
Acquity Uplc

Introduction: A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC) method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances Materials and Methods: Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno, Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute, 4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of 2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to 101.23±1.39% for Paracetamol. Results and Discussion: The validated optimal protocol is robust and accurate for simultaneous analysis of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as pharmaceutical formulation. Conclusion: In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical preparations.

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