scholarly journals Voltammetric bienzymatic sensor for sucrose determination in honey

2021 ◽  
Vol 16 (2) ◽  
pp. 61-70
Author(s):  
A. Kornii ◽  
A. Borets ◽  
O. Tananaiko
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Analytical Signal ◽  
Sio2 Film ◽  
Calibration Graph ◽  
Carbon Electrodes ◽  
Electroassisted Deposition ◽  
Biocomposite Film ◽  
Planar Carbon

An electrochemical sensor based on nanostructured planar carbon electrodes (nanoSPCE) modified with a SiO2 biocomposite film containing MnO2 particles and enzymes glucose oxidase and invertase was developed. MnO2 particles obtained by electrochemical deposition possessed electrocatalytic activity toward hydrogen peroxide. Glucose oxidase (GOx) and invertase (Inv) were encapsulated into SiO2 film by method of electroassisted deposition. The electrode modified with biocomposite film nanoSPCE/MnO2/GoX/Inv/SiO2 was electroactive toward sucrose. The indicator reaction was oxidation of H2O2 – the product of bienzymatic reaction which was catalyzed by MnO2 on the electrode surface. The linearity range of the calibration graph for the voltametric determination of sucrose using developed modified electrode is 0.017-0.342 mg/ml, the detection limit is 0.006 mg/ml. The obtained nanoSPCE/MnO2/GoX/Inv/SiO2 electrode demonstrated satisfactory stability of the analytical signal during one month of operation. The developed method was used for sucrose determination in the honey samples. The 50-fold molar excess of glucose and fructose does not interfere the determination of sucrose.

scholarly journals DETERMINATION OF GLUCOSE OXIDASE ACTIVITY BY SPECTROPHOTOMETRIC METHOD

2021 ◽  
pp. 18-25
Author(s):  
Борис Борисович Тихонов ◽  
Полина Юрьевна Стадольникова ◽  
Александр Иванович Сидоров ◽  
Михаил Геннадьевич Сульман
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Oxidase Activity ◽  
Calibration Graph ◽  
Ammonium Molybdate ◽  
Initial Glucose Concentration ◽  
Reproducible Method ◽  
Hydrogen Peroxide Formation ◽  
Glucose Oxidation Reaction

В статье рассматривается универсальная, чувствительная, быстрая и воспроизводимая методика определения активности глюкозооксидазы, основанная на окислении пероксидом водорода йодида калия в присутствии молибдата аммония и фотометрировании образующегося синего комплекса «йод-крахмал». Построен калибровочный график для определения концентрации пероксида водорода в реакционной смеси. Проведен анализ образования пероксида водорода в реакции окисления глюкозы глюкозооксидазой при варьировании начальной концентрации глюкозы. The article developed a universal, sensitive, fast and reproducible method for determining glucose oxidase activity, based on the oxidation of potassium iodide by hydrogen peroxide in the presence of ammonium molybdate and photometry of the resulting blue iodine-starch complex. A calibration graph is constructed to determine the concentration of hydrogen peroxide in the reaction mixture. Analysis of hydrogen peroxide formation in glucose oxidation reaction with glucose oxidase at variation of initial glucose concentration was performed.

Study on Determination of Glucose in Amylofermentation Liquid Using Ultraviolet Spectrophotometry

2012 ◽  
Vol 538-541 ◽  
pp. 2434-2437 ◽  
Author(s):  
Bao Shan He ◽  
Na Gao ◽  
Fang Wei ◽  
Qi Yu Lu
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Optical Method ◽  
Maximum Absorption ◽  
Ultraviolet Spectrophotometry ◽  
Titanyl Oxalate ◽  
Maximum Absorption Wavelength ◽  
Absorption Wavelength

In the presence of glucose oxidase, glucose in samples was oxygenated to hydrogen peroxide, the solution turned from colourless to yellow upon the reaction of potassium titanyl oxalate to the generated hydrogen peroxide. Using ultraviolet spectrophotometry, a new optical method for detecting glucose in amylofermentation liquid has been established. Results demonstrated that glucose concentrations were proportional to absorbance at the maximum absorption wavelength of 380 nm. A favorable linearity was presented in the range of 1 mmol/L to 60 mmol/L. The linear coeffciency was 0.993. This method was simple, reliable, and could be used for determing glucose in samples.

scholarly journals The Coupling of Cellobiase and Peroxidase by Glucose Oxidase

1961 ◽  
Vol 14 (3) ◽  
pp. 489 ◽  
Author(s):  
MA Jermyn
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
High Specificity

Youatt (1958) has described a method for estimating cellobiase activity that uses the coupled glucose oxidase-catalase system to determine manometrically the amount of glucose liberated from cellobiose by the enzyme. The high specificity of glucose oxidase makes it the reagent of choice for the otherwise difficult determination of glucose in the presence of excess cellobiose. The hydrogen peroxide produced by glucose oxidase can, however, also be demonstrated qualitatively by the use of peroxidase and both Eyer, Linzenmeier, and von Schrader (1957) and Huggett and Nixon (1957) have described sensitive quantitative glucose oxidase-peroxidase systems for determining glucose as such.

scholarly journals Kinetic spectrophotometric determination of Co(II) ion by the oxidation of ponceau 4R by hydrogen peroxide

2006 ◽  
Vol 71 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Zora Grahovac ◽  
Snezana Mitic ◽  
Emilija Pecev ◽  
Snezana Tosic
Keyword(s):  
Hydrogen Peroxide ◽  
Kinetic Method ◽  
Calibration Graph ◽  
Borate Buffer ◽  
Linear Calibration ◽  
Reaction Conditions ◽  
Ponceau 4R ◽  
Optimum Reaction ◽  
Kinetic Spectrophotometric

Anew, sensitive and simple kinetic method has been developed for the determination of traces of Co(II) ions based on their catalytic effect in the oxidation of trisodium-2-hydroxy-1-(4-sulphonato-1-naphthylazo)naphthalene-6,8-disulphonate (red artificial color Ponceau 4R) by hydrogen peroxide in borate buffer. The reaction was followed spectrophotometrically by tracing the oxidation product at 478.4 nm within 1 min after the initiation of the reaction. The optimum reaction conditions are: borate buffer (pH 10.50), Ponceau 4R (8 x10-6 mol/dm3), H2O2 (3 x10-2 mol/dm3) at 22 ?C. Following this procedure, Co(II) can be determined with a linear calibration graph up to 1.17 ng/cm3 and a detection limit of 0.20, based on the 3??criterion. The relative error ranges between 4.80-3.25 % for the concentration interval of Co(II) ions 1.76-17.61 ng/cm3. The effects of certain foreign ions on the reaction rate were determined for an assessment of the selectivity of the method. The method was applied for the determination of Co(II) in pharmaceutical samples.

A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Consumption

1968 ◽  
Vol 14 (2) ◽  
pp. 116-131 ◽  
Author(s):  
Arnold Henry Kadish ◽  
Robert L Litle ◽  
James C Sternberg
Keyword(s):  
Hydrogen Peroxide ◽  
Oxygen Consumption ◽  
Glucose Oxidase ◽  
Oxygen Sensor ◽  
Direct Measure ◽  
Peroxide Formation ◽  
Glucose Levels ◽  
Rate Of Oxygen Consumption ◽  
Hydrogen Peroxide Formation

Abstract Glucose levels in serum, plasma, and urine are determined rapidly and conveniently by a new glucose oxidase method employing a polarographic oxygen sensor with a circuit modified to record the rate of oxygen consumption. The maximum apparent rate of oxygen consumption relative to the rate obtained with a glucose standard provides a direct measure of the glucose level in the sample; results are obtainable within 20 sec. after sample (100 µl.) addition and within 3 min. after a blood sample is withdrawn from a patient. Interferences associated with prior colorimetric glucose oxidase methods are avoided by measuring oxygen consumption instead of hydrogen peroxide formation. The method is described and results are presented showing a standard deviation of less than 1.5% on replicate determinations and a bias of 1% with respect to data obtained on the same samples by the automated ferricyanide method.

scholarly journals DETERMINATION OF VULCANIZATION ACCELERATORS IN ANALYSIS OF POLYMER MATERIALS BY HPLC

2018 ◽  
Vol 54 (3) ◽  
pp. 289-295
Author(s):  
A. V. Yukhnik ◽  
S. M. Leschev
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Analytical Signal ◽  
Calibration Graph ◽  
Polymer Materials ◽  
Internal Diameter ◽  
Aqueous Extracts ◽  
Gradient Separation ◽  
Test Objects

A method of simultaneous determination of altax, captax, thiuram D, thiuram E, thimate and ethylthimate in aqueous extracts in the sanitary-chemical analysis by high performance liquid chromatography has been developed. Determination was based on the gradient separation of altax, captax, thiuram D, thiuram E, thimate, ethylthimate extracted by water from test objects using Waters XTerra MS C18 column of 250 mm length, internal diameter 4.6 mm, graining phase 5 μm, while UV detection wavelengths were 265 nm and 320 nm. Retention times were 10.3±0.2 min for altax, 3.6±0.2 min for captax, 9.0±0.2 min for thiuram D, 12.3±0.2 min for thiuram E, 11.0±0.2 min for thimate, 15.5±0.2 min for ethylthimate. It has been shown that the method is linear in the range of 0.05–0.60 mkg/ml for altax, 0.005–0.60 mkg/ml for captax, 0.005–0.75 mkg/ml for thiuram D and thiuram Е, 0.01–0.90 mkg/ml for thimate and ethylthimate. Using the calibration graph and standard deviations of analytical signal, following limits of quantification were calculated: 0.01 mkg/ml for altax, 0.002 mkg/ml for captax, 0.003 mkg/ml for thiuram D, 0.005 mkg/ml for thiuram Е, 0.01 mkg/ml for thimate and ethylthimate.

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