scholarly journals Effects of Low Temperature on Concentrations of Soluble Sugars and Quercitol of Cork Oak Seedlings

2018 ◽  
Keyword(s):  
Low Temperature ◽  
Soluble Sugars ◽  
Cork Oak ◽  
Oak Seedlings

scholarly journals The Brachypodium distachyon cold-acclimated plasma membrane proteome is primed for stress resistance

2021 ◽  
Author(s):  
Collin L Juurakko ◽  
Melissa Bredow ◽  
Takato Nakayama ◽  
Hiroyuki Imai ◽  
Yukio Kawamura ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Low Temperature ◽  
Cold Acclimation ◽  
Stress Responses ◽  
Brachypodium Distachyon ◽  
Soluble Sugars ◽  
Economic Losses ◽  
Plasma Membrane Proteins ◽  
Membrane Proteome

Abstract In order to survive sub-zero temperatures, some plants undergo cold acclimation where low, non-freezing temperatures and/or shortened day lengths allow cold hardening and survival during subsequent freeze events. Central to this response is the plasma membrane, where low-temperature is perceived and cellular homeostasis must be preserved by maintaining membrane integrity. Here, we present the first plasma membrane proteome of cold-acclimated Brachypodium distachyon, a model species for the study of monocot crops. A time course experiment investigated cold acclimation-induced changes in the proteome following two-phase partitioning plasma membrane enrichment and label-free quantification by nano-liquid chromatography mass spectrophotometry. Two days of cold acclimation were sufficient for membrane protection as well as an initial increase in sugar levels, and coincided with a significant change in the abundance of 154 proteins. Prolonged cold acclimation resulted in further increases in soluble sugars and abundance changes in more than 680 proteins, suggesting both a necessary early response to low-temperature treatment, as well as a sustained cold acclimation response elicited over several days. A meta-analysis revealed that the identified plasma membrane proteins have known roles in low-temperature tolerance, metabolism, transport, and pathogen defense as well as drought, osmotic stress and salt resistance suggesting crosstalk between stress responses, such that cold acclimation may prime plants for other abiotic and biotic stresses. The plasma membrane proteins identified here present keys to an understanding of cold tolerance in monocot crops and the hope of addressing economic losses associated with modern climate-mediated increases in frost events.

Temperature-related shifts in soluble carbohydrate content during dormancy and cold acclimation in Populus tremuloides

2001 ◽  
Vol 31 (4) ◽  
pp. 730-737 ◽  
Author(s):  
Samuel E Cox ◽  
Cecil Stushnoff
Keyword(s):  
Low Temperature ◽  
Cold Acclimation ◽  
Populus Tremuloides ◽  
Cold Hardiness ◽  
Soluble Sugars ◽  
Woody Species ◽  
Early Spring ◽  
Late Winter ◽  
Soluble Carbohydrate ◽  
Scanning Calorimetry

Changes in metabolism that accompany cold acclimation and deacclimation, such as increasing levels of raffinose family oligosacharides (RFO) during cold acclimation demonstrated in several woody species, are of interest in a search for genetic control of environmental adaptation by cold-hardy woody plants. This study examined the relationship of temperature to endodormancy and cold hardiness in trembling aspen (Populus tremuloides Michx.) buds collected at 1560, 2250, and 2900 m elevation near Fort Collins, Colo. Buds from all sites tolerated at least –85°C in December, and buds from 2900 m, the highest elevation, hardened most quickly in fall and retained their hardiness the longest in late winter and early spring. Exposure to liquid nitrogen caused bud break in normally endodormant (15 November collection date) buds. RFO levels were highly correlated to low temperature during acclimation and to lowest survival temperatures. Endogenous raffinose and stachyose increased as temperatures dropped in early winter and diminished as temperatures rose in spring. Arrhenius plots showed that raffinose accumulation was strongly low-temperature dependent during acclimation. Its loss, while also temperature dependant in spring, was not as pronounced as during fall acclimation. Buds from all three sites survived cryopreservation at –196°C when first prefrozen at 5°C/h and stored >4 h at –20°C or colder. Differential scanning calorimetry data suggest that an aqueous component froze separately from tissues that underwent a glass transition in buds that survived cryopreservation. This study documents a complete dormant season hardiness profile of aspen linking hardiness with changes in endogenous soluble sugars.

scholarly journals The Interaction of Photoperiod and Temperature on Cold Hardiness and the Accumulation of Soluble Sugars in Two Cultivars of Southern Magnolia (Magnolia grandiflora L.)

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 848F-848
Author(s):  
C.L. Haynes ◽  
O.M. Lindstrom ◽  
M.A. Dirr
Keyword(s):  
Low Temperature ◽  
Cold Acclimation ◽  
Low Temperatures ◽  
Cold Hardiness ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Total Soluble Sugars ◽  
Magnolia Grandiflora ◽  
Temperature Interaction

Decreasing photoperiods and decreasing temperatures induce cold acclimation and the accumulation of soluble sugars in many plants. Two cultivars of southern magnolia differing in cold hardiness and acclimation patterns, were monitored to determine photoperiod × temperature interaction on cold hardiness and soluble sugar content. Cold hardiness increased with low temperatures and short photoperiods. Total soluble sugars, sucrose, and raffinose consistently increased in the leaves and stems of both cultivars in response primarily to low temperature. `Little Gem' was less responsive to photoperiod than `Claudia Wannamaker'

scholarly journals Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1573
Author(s):  
Marija Marković ◽  
Milana Trifunović Momčilov ◽  
Branka Uzelac ◽  
Olga Radulović ◽  
Snežana Milošević ◽  
...  
Keyword(s):  
Low Temperature ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Temperature Treatment ◽  
Sugar Concentration ◽  
Total Soluble Sugars ◽  
Low Temperature Treatment ◽  
Lower Glucose ◽  
Different Parts

The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.

scholarly journals The Brachypodium distachyon cold-acclimated plasma membrane proteome is primed for stress resistance

2021 ◽  
Author(s):  
Collin L Juurakko ◽  
Melissa Bredow ◽  
Takato Nakayama ◽  
Hiroyuki Imai ◽  
Yukio Kawamura ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Low Temperature ◽  
Cold Acclimation ◽  
Stress Responses ◽  
Brachypodium Distachyon ◽  
Soluble Sugars ◽  
Economic Losses ◽  
Plasma Membrane Proteins ◽  
Membrane Proteome

Abstract In order to survive sub-zero temperatures, some plants undergo cold acclimation where low, non-freezing temperatures and/or shortened day lengths allow cold hardening and survival during subsequent freeze events. Central to this response is the plasma membrane, where low-temperature is perceived and cellular homeostasis must be preserved by maintaining membrane integrity. Here, we present the first plasma membrane proteome of cold-acclimated Brachypodium distachyon, a model species for the study of monocot crops. A time course experiment investigated cold acclimation-induced changes in the proteome following two-phase partitioning plasma membrane enrichment and label-free quantification by nano-liquid chromatography mass spectrophotometry. Two days of cold acclimation were sufficient for membrane protection as well as an initial increase in sugar levels, and coincided with a significant change in the abundance of 154 proteins. Prolonged cold acclimation resulted in further increases in soluble sugars and abundance changes in more than 680 proteins, suggesting both a necessary early response to low-temperature treatment, as well as a sustained cold acclimation response elicited over several days. A meta-analysis revealed that the identified plasma membrane proteins have known roles in low-temperature tolerance, metabolism, transport, and pathogen defense as well as drought, osmotic stress and salt resistance suggesting crosstalk between stress responses, such that cold acclimation may prime plants for other abiotic and biotic stresses. The plasma membrane proteins identified here present keys to an understanding of cold tolerance in monocot crops and the hope of addressing economic losses associated with modern climate-mediated increases in frost events.

scholarly journals Responses of Broccoli Seedlings to Light Quality during Low-temperature Storage in Vitro: II. Sugar Content and Photosynthetic Efficiency

HortScience ◽  
1998 ◽  
Vol 33 (7) ◽  
pp. 1258-1261 ◽  
Author(s):  
Sandra B. Wilson ◽  
Keiko Iwabuchi ◽  
Nihal C. Rajapakse ◽  
Roy E. Young
Keyword(s):  
Low Temperature ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Photon Flux ◽  
Media Composition ◽  
Low Temperature Storage ◽  
Photosynthetic Ability ◽  
Temperature Storage

Broccoli (Brassica oleracea L. Botrytis group `Green Duke') seeds were cultured photoautotrophically (without sugar) or photomixotrophically (with sugar) in vitro for 3 weeks at 23 °C and150 μmol·m-2·s-1 photosynthetic photon flux (PPF). In vitro seedlings were stored for 0, 4, 8, or 12 weeks at 5 °C in darkness or under 5 μmol·m-2·s-1 of white (400–800 nm), blue (400–500 nm), or red (600–700 nm) light. Photosynthetic ability and soluble sugar contents were determined after removal from storage. Photomixotrophic seedlings contained approximately five times more soluble sugars than did photoautotrophic seedlings. Dark storage reduced soluble sugars in both photoautotrophic and photomixotrophic plants, but photosynthetic ability was maintained for up to 8 weeks in the latter whereas it decreased in the former. Illumination in storage increased leaf soluble sgars in both photoautotrophic and photomixotrophic seedlings. Soluble sugars in stems decreased during storage regardless of illumination, but remained higher in illuminated seedlings. Red light was more effective in increasing or maintaining leaf and stem soluble sugars than was white or blue light. Regardless of media composition or illumination, storage for more tan 8 weeks resulted in dramatic losses in quality and recovery, as well as photosynthetic ability. Seedlings stored for 12 weeks comletely lost their photosynthetic ability regardless of media composition or illumination. The results suggest that carbohydrate, supplied in the media or through illumination, is essential for maintenance of photosynthetic ability during low-temperature storage for up to 4 or 8 weeks.

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