scholarly journals Resposta da Fisális (Physalis peruviana L.) à inoculação e coinoculação de microrganismos promotores de crescimento de plantas

2020 ◽  
Vol 13 (3) ◽  
pp. 915-932
Author(s):  
Felix Cidade do Prado ◽  
Rodrigo Ferraz Ramos ◽  
Eloi Evandro Delazeri ◽  
Cristiano Bellé ◽  
Daniel Joner Daroit ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Azospirillum Brasilense ◽  
Trichoderma Asperellum ◽  
Chl A ◽  
Physalis Peruviana

Objetivou-se avaliar a resposta de Physalis peruviana L. frente à inoculação e coinoculação de diferentes microrganismos promotores de crescimento de planta (MPCP) em condição de campo. O delineamento foi em blocos casualizados, sendo quatro blocos e oito tratamentos. Os tratamentos do experimento referem-se à inoculação individual de Azospirillum brasilense (A), Bacillus amyloliquefaciens (B) e Trichoderma asperellum (T), diferentes combinações com esses MPCPs (T+B; T+A; B+A e T+A+B) e um tratamento controle (C). Aos 15 dias após o transplantio das mudas (DAT) avaliou-se os índices SPAD de clorofilas (Chl a, Chl b e Chl total) e aos 50 e 150 DAT avaliou-se a altura da parte aérea (APA). Os frutos foram colhidos aos 75 DAT até 120 DAT, sendo determinado o número de frutos por planta (NFP) e a massa média de frutos (MF). Amostras dos frutos foram submetidas a análises físico-químicas, com determinação de luminosidade (L*), cromaticidade (c*) e coloração externa (ºhue) dos frutos. Aos 270 DAT determinou-se a biomassa fresca da parte aérea (BFPA) e biomassa seca da parte aérea (BSPA). Não foram observadas diferenças significativas para as variáveis analisadas. As coinoculações de B+A e A+T apresentam as maiores médias para Chl a, enquanto que a combinação de T+A+B apresentou maiores médias para Chl total, ambos diferindo estatisticamente da inoculação de A. brasilense. Para a variável ºhue os tratamentos A, B e T+B apresentaram as menores médias, resultando melhor aspecto visual da coloração de frutos.

scholarly journals Crescimento, desenvolvimento e aspectos produtivos do morangueiro submetido a inoculações com bacillus amyloliquefaciens, azospirillum brasilense e trichoderma asperellum com e sem associação ao silício

2019 ◽  
Vol 22 (1) ◽  
pp. 131-146
Author(s):  
Hislley Campos Soares Bubanz ◽  
Rodrigo Ferraz Ramos ◽  
Débora Leitzke Betemps ◽  
Lisiane Sobucki ◽  
Mariana Ferneda Dossin ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Azospirillum Brasilense ◽  
Trichoderma Asperellum

Objetivou-se avaliar o crescimento, desenvolvimento e aspectos produtivos do morangueiro submetidos a inoculação de Azospirillum brasilense, Bacillus amyloliquefaciens e Trichoderma asperellum com e sem associação ao Silício (Si). O delineamento experimental foi blocos casualizados, com oito tratamentos, três blocos, sendo três repetições por tratamento para cada bloco. Realizou-se avaliações de crescimento e desenvolvimento aos 30 e 60 dias após o transplantio (DAT) das mudas. Para as avaliações dos aspectos produtivos, os frutos maduros coletados foram submetidos as análises físico-químicas. Aos 150 DAT avaliou-se o acúmulo de biomassa seca, através da determinação da massa seca da parte aérea (MSA), massa seca da coroa (MSC), massa seca das raízes (MSR) e massa seca total (MST). Aos 30 DAT não foi observada diferença significativa entre os tratamentos. Aos 60 DAT os tratamentos B. amyloliquefaciens (T3) e Si (T5) apresentaram os maiores índices de clorofila (IC). Os tratamentos T. asperellum e Si (T6), T3 e T5 apresentaram as maiores médias para MSA, enquanto que o tratamento T5 apresentou a maior média para MSC e o tratamento T6 apresentou as maiores médias para MSR e MST. Conclui-se que os tratamentos influenciaram o crescimento, desenvolvimento e acúmulo de biomassa seca no morangueiro, não apresentando influência nos parâmetros físico-químico dos frutos. 

scholarly journals Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Valliappan Karuppiah ◽  
Lu Zhixiang ◽  
Hongyi Liu ◽  
Murugappan Vallikkannu ◽  
Jie Chen
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Corn Stover ◽  
Bacillus Amyloliquefaciens ◽  
Genetically Engineered ◽  
Cellulolytic Enzymes ◽  
Regulation Of Transcription ◽  
Trichoderma Asperellum ◽  
Biomass Hydrolysis ◽  
Lignocellulose Biodegradation

Abstract Background Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.

scholarly journals Effectiveness of the Biological Control of Garlic (Allium sativum L.)

2016 ◽  
Vol 19 (s1) ◽  
pp. 15-17
Author(s):  
Jacek Nawrocki ◽  
Anna Pogodzińska
Keyword(s):  
Biological Control ◽  
Health Status ◽  
Root Rot ◽  
Bacillus Amyloliquefaciens ◽  
Allium Sativum ◽  
Field Experiments ◽  
First Year ◽  
Trichoderma Asperellum ◽  
Pythium Oligandrum ◽  
Second Year

Abstract In two-year field experiments (2014 and 2015), the effect of used preparations on health status of leaves and roots and bulbs on two cultivars of garlic: ‘Arkus’ and ‘Garpek’ was studied. During investigations: Polyversum WP (Pythium oligandrum), Trifender WP (Trichoderma asperellum) and RhizoVital 42 (Bacillus amyloliquefaciens) and standard fungicide Topsin M 500 SC (tiophanate methyl) were used. Unprotected plants presented control. The obtained results showed that in the first year of the studies, all the tested formulations effectively protected the roots and bulbs of garlic against rot, except RhizoVital 42 for ‘Arkus’ variety and Trifender WP for cultivar ‘Garpek’. In 2015, all tested preparations, without exception, limited root rot and the rot of basal part of bulbs both cultivars of garlic. The applied biological preparations had no significant effect on health of the leaves of garlic in 2014 and in 2015 for cultivar ‘Garpek’, while in the second year of studies, all the tested formulations effectively limited the dieback of leaves of the garlic cultivar ‘Arkus’.

scholarly journals Glyceolin and phaseolin induction in soybean and beans as a function of biocontrol agents application

2019 ◽  
Vol 6 (1) ◽  
pp. 113-118
Author(s):  
Marilia Gervásio da Costa Ferreira ◽  
Neilson De Oliveira Borges ◽  
Ricardo Francischini ◽  
Jair Pereira de Melo Júnior ◽  
Antônio Jussiê Da Silva Solino ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Integrated Management ◽  
Longitudinal Section ◽  
Lower Surface ◽  
Resistance Mechanisms ◽  
Biocontrol Agents ◽  
Cultivated Plants ◽  
Trichoderma Asperellum ◽  
Regression Test ◽  
Soybean Plants

Induction of plant resistance is a tool that can be incorporated into the integrated management of diseases of cultivated plants. In this way, this work aims to evaluate the elicitor action of biocontrol agents in the induction of glyceolin. In this study were used concentrations about 0; 0.5; 1; 1.5 and 2% diluted in water; spore suspension of Trichoderma asperellum BV10, Bacillus subtilis BV02 and Bacillus amyloliquefaciens BV03 microorganisms. To determine the glyceolin, cotyledons were grown in sand, weighed and cut in longitudinal section on the lower surface. Subsequently, these were deposited 50 μL of the concentrations in the cuts and the extraction performed in H2O and concentration determined by absorbance at wavelength 285nm. The results were submitted to analysis of variance and compared by the regression test (p <0.05). When measuring the glyceolin in soybean cotyledons, there was an increase in the accumulation of this phytoalexins as the concentration of B. subtilis BV02 cells increased. The 4% concentration of B. subtilis BV02 promoted 237% more glyceolin accumulation in soybean cotyledons than the control. The accumulation of glyceolin was increased as the concentration of T. asperellum BV10 cells increased, so that 4% concentration promoted 228% more glycerol accumulation than the control. Increasing the concentration of Bacillus amyloliquefaciens BV03 cells increased the accumulation of this phytoalexins. The 4% concentration promoted 129% more accumulation of glycerol in soybean cotyledons than the control. The microorganisms T. asperellum, B. subtilis and B. amyloliquefaciens are indicated as activators of resistance mechanisms of soybean plants.

scholarly journals Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

2020 ◽  
Author(s):  
Valliappan Karuppiah ◽  
Lu Zhixiang ◽  
Hongyi Liu ◽  
Murugappan Vallikkannu ◽  
Jie Chen
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Corn Stover ◽  
Bacillus Amyloliquefaciens ◽  
Genetically Engineered ◽  
Cellulolytic Enzymes ◽  
Regulation Of Transcription ◽  
Trichoderma Asperellum ◽  
Biomass Hydrolysis ◽  
Lignocellulose Biodegradation

Abstract Background: Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results: The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion: The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.

scholarly journals Sources and Doses of Nitrogen Associated with Inoculation with Azospirillum brasilense Modulate Growth and Gas Exchange of Corn in the Brazilian Amazon

2021 ◽  
Vol 26 (02) ◽  
pp. 349-358
Author(s):  
Juscelino Gonçalves Palheta
Keyword(s):  
Stomatal Conductance ◽  
Gas Exchange ◽  
Vegetative Growth ◽  
Azospirillum Brasilense ◽  
Dry Mass ◽  
N Fertilization ◽  
Stem Diameter ◽  
Urease Inhibitor ◽  
Chl A ◽  
Specific Objective

The specific objective of the study was to evaluate effect of inoculation with Azospirillum brasilense and nitrogen (N) doses on vegetative growth and gas exchange in Zea mays L. The experimental design adopted was the completely randomized, in a 4 2 2 factorial scheme, in the following way: four doses of N (0 60 120 and 180 kg ha-1 of N), two sources of N (common urea and urease inhibitor-treated urea) and absence and presence of inoculation with A. brasilense, with four replications. The evaluations were made for vegetative growth of the plant (plant height, stem diameter, leaf area, number of leaves, dry mass of stem, root, leaves and aerial part and total dry mass) and photosynthesis, stomatal conductance, transpiration, internal carbon, relationship between internal and external carbon and content of chloroplast pigments. The application of N provided an improvement in plant growth, and, in general, the dose of 180 kg ha-1 N associated with A. brasilense, promoted an increase in stem diameter, photosynthesis, stomatal conductance, transpiration and internal carbon ratio of the corn. The treatment with urease inhibitor, greatly promoted the stem diameter, transpiration, Ci/Ca ratio and chlorophyll (Chl) a, b, total compared to urea treatment. The inoculation of the corn seeds with the bacteria and the use of N fertilization, regardless of the source, promoted an improvement in the vegetative growth of the hybrid, improving the vegetative growth and the physiological responses of corn when applied to the highest dose of 180 kg/ha N. © 2021 Friends Science Publishers

scholarly journals Eficiência de produtos biológicos na coinoculação de sementes de soja

2021 ◽  
Vol 2 (2) ◽  
pp. e21109
Author(s):  
Luana Patrícia Pinto Korber ◽  
Ângelo Henrique Canan Korber ◽  
Luciana Grange ◽  
Celestina Alflen Klahold
Keyword(s):  
Bacillus Subtilis ◽  
Bradyrhizobium Japonicum ◽  
Bacillus Amyloliquefaciens ◽  
Azospirillum Brasilense ◽  
Bacillus Pumilus ◽  
Bacillus Methylotrophicus

O objetivo deste trabalho foi verificar o potencial uso de produtos biológicos comerciais na coinoculação com Bradyrhizobium japonicum no tratamento de semente da soja. O experimento foi realizado em papel germitest e o cultivo em vasos. Foram realizados sete tratamentos com cinco repetições, sendo: T1 – testemunha, T2 –Bradyrhizobium japonicum, T3 – B. japonicum + Azospirillum brasilense, T4 – B. japonicum + Bacillus subtilis, T5 – B. japonicum + Bacillus amyloliquefaciens, T6 – B. japonicum + Bacillus methylotrophicus e T7 – B. japonicum + Bacillus pumilus. As variáveis analisadas foram germinação (G), germinação de plântulas normais (GN), índice de velocidade de emergência (IVE), crescimento parte aérea em vaso (CAV) e papel germitest (CAG), de raíz em vaso (CRV) e papel germitest (CRG). Os dados foram submetidos a análise dos pressupostos e determinados a partir do teste de Tukey a 5% de probabilidade e realização da análise de componentes principais (ACP). A coinoculação de B. japonicum + A. brasilense promoveu ganhos de enraizamento e germinação em plântulas de soja. A coinoculação com B. pumilus apresentou potencial de efeito semelhante a associação de B. japonicum + A. brasilense. A associação com B. methylotrophicus apresentou melhor enraizamento em vaso (CRV) que os demais tratamentos. O uso de BPCP’s em associação com B. japonicum potencializa o desenvolvimento inicial da plântula.

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