scholarly journals Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection

2015 ◽  
Vol 2 (4) ◽  
pp. 31990-31990 ◽  
Author(s):  
Fariha Akhter Chowdhury ◽  
Mohammad Nurul Islam ◽  
Anamika Saha ◽  
Sabrina Mahboob ◽  
Abu Syed Md. Mosaddek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples

scholarly journals Antibiotic Resistance Pattern of Extended Spectrum Beta Lactamase Producing Escherichia coli Isolated From Patients With Urinary Tract Infection in Morocco

2021 ◽  
Vol 11 ◽  
Author(s):  
Mohamed Kettani Halabi ◽  
Fatima Azzahra Lahlou ◽  
Idrissa Diawara ◽  
Younes El Adouzi ◽  
Rabiaa Marnaoui ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Resistance Pattern ◽  
E Coli ◽  
Antibiotic Resistance Pattern ◽  
Extended Spectrum

Extended-spectrum β-lactamases producing Escherichia coli (ESBL-EC) lend resistance to most β-lactam antibiotics. Because of limited treatment options, ESBL-EC infections are generally more difficult to treat, leading to higher hospital costs, reduced rates of microbiological and clinical responses, and a threat to the patient’s life. This study aimed to determine the antibiotic resistance pattern of ESBL-EC isolated from patients with urinary tract infection in Morocco. This retrospective laboratory-based study was conducted at Cheikh Khalifa International University Hospital, Casablanca, from January 2016 to June 2019. A total of 670 urine samples were collected from urinary tract infection patients and processed by standard microbiological methods. In vitro susceptibility testing to different antibiotics of all identified isolates of Escherichia coli (E. coli) was performed following Kirby–Bauer’s disc diffusion method on Mueller–Hinton Agar according to the EUCAST standards. The reviewing of ESBL-EC was confirmed by the appearance of a characteristically shaped zone referred to as a “champagne cork” using the Combined Disk Test. Among a total of 438 E. coli isolated from nonrepetitive urine samples, two hundred fifty-nine (59%) were ESBL-EC, of which 200 (77%) were isolated from adult patients (over the age of 50) and the majority were female. All ESBL-EC isolates were resistant to third-generation cephalosporin and quinolones and sensitive to carbapenem and fosfomycin. Knowledge of antimicrobial resistance patterns in ESBL-EC, the major pathogen associated with urinary tract infection, is indispensable as a guide in choosing empirical antimicrobial treatment.

scholarly journals Antimicrobial Resistance in Urinary Isolates of Escherichia coli with Special Reference to Fluoroquinolone Resistance and Extended Spectrum Beta-Lactamase (ESBL) Production

2021 ◽  
Vol 10 (9) ◽  
pp. 583-588
Author(s):  
Jyoti Rajowar ◽  
Sangeeta Dey Akoijam ◽  
Aninda Sen ◽  
Kahkashan Akhter ◽  
Shreshy Singh
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Fluoroquinolone Resistance ◽  
Beta Lactamase ◽  
Esbl Production ◽  
Extended Spectrum Beta Lactamase ◽  
Esbl Producers

BACKGROUND The commonest bacterial agent involved in causation of urinary tract infection (UTI) is Escherichia coli, both in the community as well as in the hospital. In this study Escherichia coli strains isolated from patients with UTI were studied especially for extended spectrum beta-lactamase (ESBL) production and determination of fluoroquinolone resistance. METHODS This descriptive study was conducted in the Department of Microbiology, Katihar Medical College and Hospital from December 2018 to May 2020. Urine samples from suspected UTI cases were processed and bacterial isolates were identified as per standard protocol. Antimicrobial susceptibility testing was done by Kirby-Bauer discdiffusion method on Mueller-Hinton agar. ESBL detection was done as per Clinical and Laboratory Standards Institute (CLSI) guidelines. RESULTS Out of 3938 urine samples received in the microbiology laboratory, 708 samples showed significant growth of various bacteria and candida species, out of these only 105 patients had urinary tract infection caused by Escherichia coli. The male to female ratio was 0.25:1. Isolates were highly sensitive to nitrofurantoin (80.9 %) followed by amikacin (72.4 %) and imipenem (71.5 %). Maximum resistance was seen with amoxicillin (98.1 %), cefuroxime (96.2 %), cefpodoxime and cefotaxime (90.5 %), ceftriaxone (85.7 %), nalidixic acid (91.4 %) and ciprofloxacin (70.5 %). 70.5 % were found to be ESBL producers and 29.5 % were non-ESBL producers. The double disc synergy test (DDST) could detect only 42.8 % of ESBL producers whereas phenotypic confirmatory disc diffusion (PCDDT) detected 70.5 % of ESBL producers. CONCLUSIONS It was seen in the present study that a high proportion of community acquired strains of Escherichia coli were ESBL producers. In this study, 70.5 % of Escherichia coli strains were ESBL positive. It can therefore be recommended that all gram-negative isolates be tested for ESBL production preferably by the PCDDT test as this test was found to be most sensitive for detection of ESBL production. The PCDDT test requires minimum laboratory infrastructure, is cheap and easy as compared to molecular methods. KEY WORDS Escherichia coli, UTI, ESBL, PCDDT, DDST

scholarly journals Prevalence and comparative likelihood of urinary tract infection (UTI) among female out patients in BSMMU

2013 ◽  
Vol 40 (2) ◽  
pp. 231-239
Author(s):  
Hamida Khanum ◽  
Fahmida Munir ◽  
AZM Shafiullah ◽  
Farhana Muznebin
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Relative Risk ◽  
Tract Infection ◽  
Old Age ◽  
Middle Age ◽  
Age Groups ◽  
Urine Samples ◽  
E Coli

Out of 200 urine samples of female out patients from BSMMU hospital, 128 (64%) cases were detected as Escherichia coli positive, 66.67% of cases in summer and 60% in winter. To compare the risks of E. coli infection in summer and winter and also among different age groups, odd ratio (OR) and relative risk (RR) were used as measures of comparative likelihood. The values of both OR and RR were greater than one when the risk of E. coli infection of adults (16-35) was compared with that of middle age (36-50) and old age (51-70) groups. The adults were always at higher risk of UTI by E. coli, and the livelihood of infection was not equal in summer and winter. DOI: http://dx.doi.org/10.3329/bjz.v40i2.14317 Bangladesh J. Zool. 40(2): 231-239, 2012

scholarly journals Asymptomatic Bacteriuria among Pregnant Women Attending a Tertiary Care Hospital in Western Nepal: A Cross-sectional Prospective Study

2021 ◽  
Vol 9 (01) ◽  
pp. 38-42
Author(s):  
Shristi Raut ◽  
Sulochana Khatiwada ◽  
Narayan GC
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Asymptomatic Bacteriuria ◽  
Urine Samples ◽  
Diffusion Method ◽  
Care Hospital ◽  
Significant Bacteriuria

INTRODUCTION Urinary tract of female undergoes tremendous changes during pregnancy which increases their risk of acquiring infection. Asymptomatic bacteriuria is a common entity among pregnant women which refers to significant bacteriuria (>105 bacteria per ml of urine) without any typical symptoms of urinary tract infection. Asymptomatic bacteriuria can lead to various maternal and fetal complications if not detected and treated on time. MATERIAL AND METHODS Total 280 urine samples were collected in sterile universal containers from pregnant women not showing typical symptoms of urinary tract infection at the time of sample collection. Urine samples were inoculated in both MacConkey agar and Blood agar by semi quantitative culture method. Culture plates were reported after 24 hours of incubation at 370C. Bacteria isolated were subjected to antibacterial susceptibility testing by modified Kirby-Bauer disc diffusion method. RESULTS Out of 280 urine cultures, 213 samples were sterile. Significant bacteriuria was seen in 25 cases (8.9%) followed by insignificant bacteriuria (20, 7.14%) and contamination (17, 6.10%). Highest number of cultures positive were in age group 21-30 years (19, 9.1%,). Out of 25 cases of significant bacteriuria, 60% were primigravida and 40% were multigravida. The most common organism isolated was Escherichia coli (10, 60%) followed by Klebsiella pneumoniae (5, 40%). CONCLUSION Screening of all pregnant women for asymptomatic bacteriuria is essential during their antenatal checkup. Escherichia coli is the commonest organism that cause asymptomatic bacteriuria. Appropriate antibiotic therapy is absolutely necessary for positive cases on the basis of antimicrobial susceptibility result to prevent unwanted obstetric complications.

scholarly journals Frequency and Distribution of Gram Negative Bacteria among Hospital and Community Acquired UTI Patients

2015 ◽  
Vol 1 (2) ◽  
pp. 24-26
Author(s):  
Md. Badrul Islam ◽  
Md. Abdullah Yusuf ◽  
Md. Shahjahan Chowdhury ◽  
AFM Arshaedi Sattar ◽  
Samia Afrin
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Gram Negative Bacteria ◽  
Klebsiella Species ◽  
Gram Negative ◽  
Pseudomonas Species ◽  
Community Patients

Background: Gram negative bacteria create a great problem during the treatment of urinary tract infection patients. Objective: This study was undertaken to determine the frequency and distribution of Gram negative bacteria among the UTI patients. Methodology: This cross sectional study was carried out in the Department of Microbiology at Sir Salimullah Medical College, Dhaka from June 2007 to May 2008 for a period of 1(one) year. All the patients presented with the clinically suspected UTI at any age with both sexes were selected as study population. Patients who were hospitalized for at least 2 days or more received different antibiotics were designated as hospital acquired UTI; on the other hand patients who were attended in OPD for the first time were considered as community acquired UTI patients. All urine samples were inoculated in Blood agar and MacConkeys agar media. Gram negative bacteria were isolated and identified by colony morphology, Gram staining and biochemical tests. Result: A total of 220 urine samples were collected from patients suspected to urinary tract infections of which 116 samples were from hospitalized patients and 104 samples were from community patients. Among 220 samples, 132(60.0%) Gram negative bacteria were isolated of which 88(66.7%) isolates were from hospitalized acquired UTI and 44(33.3%) bacteria were isolated from community acquired UTI patients. The difference was statistically significant (p=0.0001). In hospitalized patients out of 88(75.9%) isolated Gram negative bacteria, 67(76.1%) isolates were Escherichia coli, 10(11.4%) isolates were Klebsiella species, 5(5.7%) isolates were Proteus species and 6(6.8%) isolates were Pseudomonas species. Among the isolated bacteria 44(42.3%) bacterial isolates were from community patients of which 36(81.8%) isolates were Escherichia coli, 4(9.1 %) isolates were Klebsiella species, 2(4.5%) isolates were Proteus species and 2(4.5%) bacteria were Pseudomonas species. Conclusion: In the present study, it was observed that considerable numbers of Gram negative bacteria were detected from urinary tract infection cases.Bangladesh Journal of Infectious Diseases 2014;1(2):24-26

scholarly journals Pathogens isolated from clinical cases of urinary tract infection in dogs and their antibiogram

2018 ◽  
pp. 1037-1042 ◽  
Author(s):  
Manisha Punia ◽  
Ashok Kumar ◽  
Gaurav Charaya ◽  
Tarun Kumar
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Significant Proportion ◽  
Colony Growth ◽  
Multidrug Resistant ◽  
Urine Samples ◽  
Sensitivity Testing

Aim: This study aims to determine the etiology of urinary tract infection (UTI) in dogs and to develop an antibiogram of organisms isolated. Materials and Methods: Urine samples were collected either through catheterization or cystocentesis from 35 dogs suspected of UTI admitted to VCC, LUVAS, Hisar. Bacteria were identified on the basis of cultural characteristics in 22 samples, and all the isolates were subjected to in vitro antimicrobial sensitivity testing. Results: The urine samples found positive for bacteria yielded pure colony growth in 77.27% and mixed growth in 22.73% samples, respectively. Escherichia coli (29.62%) and Streptococcus spp. (29.62%) were the most prevalent microorganisms followed by Staphylococcus spp. (22.22%), Klebsiella spp. (11.11%), Pseudomonas spp. (3.7%), and Bacillus spp. (3.7%). Overall, maximum sensitivity of isolates was found toward ceftriaxone/tazobactam (88.88%) and least toward amoxicillin and cloxacillin (29.62%). Conclusion: E. coli and Streptococcus spp. were the most predominant bacteria isolated from UTI affected dogs. In vitro sensitivity revealed a significant proportion of bacteria to be multidrug resistant. Keywords: antibiogram, multidrug resistance, Escherichia coli, Staphylococci, Streptococci.

Study of Bacterial Pathogens in Urinary Tract Infection and their Antimicrobial Sensitivity Pattern

2018 ◽  
Vol 4 (2) ◽  
pp. 40-44
Author(s):  
Rezina Parveen ◽  
Ina Rahim
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Urine Samples ◽  
College Hospital ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Patient Department

Background: Urinary tract infection (UTI) is one of the most common bacterial infections in Bangladesh. Antimicrobial agents are used for its treatment. The increase in antibiotic resistance among uropathogens is a global problem.Objective: This study was designed to find out the prevalence of UTI, its causative agents and their antimicrobial susceptibility patterns among suspected patients of UTI attending Dhaka Dental College Hospital, Dhaka.Methodology: This cross-sectional study was carried out in Dhaka Dental College Hospital, Dhaka during the period of November 2014 to May 2017. Clinically diagnosed cases of UTI irrespective of age and sex from out-patient department and in-patient department were selected for the purpose of the study. For culture, the urine samples were inoculated on 5% sheep blood agar and MacConkey’s agar media using calibrated loop following standard bacteriological technique. After the incubation period, the plate were examined for bacterial pathogen. The disk diffusion method (Kirby Bauer’s) was used to determine the antimicrobial susceptibility of isolates. Standard inoculums were inoculated on Mueller-Hinton agar and incubated at 37oC for 24 hours.Result: Ninety three urine samples were studied. Among the 93 samples most of them were in the age group of 21 to 30 years. Out of 93 samples, 26 (27.95%) samples were found culture positive. Escherichia coli (92.30%) was found to be the predominant organism.  Regarding antimicrobial sensitivity pattern Esch. coli was found to be most sensitive to Imipenem (100%), Amikacin (87.5%), and Nitrofurantoin (83.33%).Conclusion: In conclusion young aged female are commonly suffering from UTI and Escherichia coli is the most common isolated bacteria.Bangladesh Journal of Infectious Diseases, December 2017;4(2):40-44

scholarly journals Causative Microorganisms and Antibiotics Susceptibilities in Children with Urinary Tract Infection

2021 ◽  
Vol 32 (1) ◽  
pp. 5
Author(s):  
Thaer M. Al-Baqer ◽  
Samar Abdul Raheem Al-Gharrawi ◽  
Noor ALHuda A. Saeed
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Salmonella Spp ◽  
Gram Negative ◽  
Antibiotic Susceptibility Test ◽  
Acinetobacter Spp ◽  
Microbial Agents

Generally, treatment of urinary tract infection (UTI) in children is a difficult task, due to development of high resistance against antibiotics. The object of this study is to investigate the greatest causative microorganisms, their susceptibility and resistance to antibiotics in children suffering from UTI in Baghdad. A total of 810 urine samples were collected from suspected cases of UTI in children patients of ages (1 day to 12 years) of both sexes. Urine specimens were examined by urinalysis and cultured for isolation of microbial agents. In this study, Bacterial growth was obtained in 202 samples (24.39%) out of 810 urine samples. Various pathogenic organisms were isolated, which represented by A) Gram-Negative bacilli include Escherichia coli (41.58%), Enterobacter spp. (13.68%), Proteus Mirabilis (12.78%), Acinetobacter spp. (4.45%), Providencia spp. (4.45%), Pseudomonas aeruginosa (3.46), Klebsiella pneumoniae (2.47%), Citrobacter spp.(0.49%), Salmonella Spp.(0.49%), B) Gram- Positive cocci: Staphylococcus aureus (11.38%), Enterococcus faecalis (1.98%) and Streptococcus agalactiae (1.48%) C) Candida albicans (0.9%).). According to antibiotic susceptibility test, mostly the isolates were resistant to Nalidixic acid (67.05%), cefotaxime (59.40%) and cefepime (50.49%). Mostly Gram-Negative bacilli were responsible for UTI, and maximum recurrent isolated bacteria were Escherichia coli. The isolated bacteria were found to be sensitive to Imipenem, Amikacin, and ciprofloxacin. Therefore, the selection of antibiotic therapy in UTI should depend on the native sensitivity form of the infecting organism.

scholarly journals Prevalence of Multidrug Resistant Escherichia Coli In Suspected Cases of Urinary Tract Infection Among Patients Attending Ahmadu Bello University Medical Center, Zaria

2020 ◽  
pp. 123-130
Author(s):  
Shitu S. ◽  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Medical Center ◽  
Urine Samples ◽  
Drug Resistant ◽  
E Coli ◽  
High Prevalence ◽  
Antibacterial Susceptibility

Urinary tract infections (UTIs) are a major health problem, the second most prevalent human bacterial infection after respiratory tract infection. Patient with urinary tract infection is a potential source of multi drug resistant (MDR) Escherichia coli (E. coli) with the potentials to spread antimicrobial resistant genes to other bacteria in the environment and other human populations. The aim of the study was to isolate and determine the prevalence of MDR Escherichia coli from patients suspected with urinary tract infection attending Ahmadu Bello University Medical Center, Zaria. A total of 95 urine samples were collected and processed according to standard microbiological methods for the isolation and identification of E. coli. Antibacterial susceptibility pattern of the isolates was determined using Kirby-Bauer’s disk diffusion technique as well as MAR indices. Out of the 95 urine samples collected 32 were from males and 63 were from females, whose ages were between 5 and 74 years. The results revealed that 35 (36.8%) out of the 95 samples collected were positive for E. coli with high prevalence among the female patients 23(24.2%) compared to the male patients 12(12.4%). High prevalence of E. coli was also reported among the patients within the age ranges of 15-24 and 25-34 years. The Escherichia coli isolates demonstrated high resistance to sparfloxacin (91.4%), followed by cotrimoxazole and amoxicillin (82.9%). Additionally, 30 (85.7%) of the isolates exhibited multi drug resistance and 94.3% (n = 33/35) had a MAR index above 0.2. The study demonstrated that some of the E. coli isolates in the study are from high-risk contaminated sources where there may be high frequency of antibiotic usage. Therefore, the study indicated the need for Physicians to prescribed antibiotics to patients following standard antibacterial susceptibility testing. Keywords: Prevalence, Escherichia coli, Multi drug resistant, Urinary tract infection

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