Birds of Parque Estadual Ilha do Cardoso: ecology, conservation and natural history

The Parque Estadual Ilha do Cardoso (PEIC), located on extreme South of São Paulo’s cost, in Brazil, holds an important Atlantic Forest remnant which still in a good state of conservation, but lack a deepen study about the avian community that habits the island. This study aimed to elaborate a census of avian species that occur in the park approaching richness, occurrence frequency, occurrence status and the structure of trophic guilds. A total of 25 field expedictions occurred between September 2015 and September 2017 during five days each, the field work included different ecosystems as mangrove, restinga, forest, sandbank, beach and marine. The census was made combine three different techniques used in ornithological studies: visual identification, auditory identification and catch by mist-nets (with five fixed sites in mangrove, restinga and forest). Were recorded 335 avian species, with 28 of them endemic from Brazil and 33 being threatened with extinction. Seventy-three species were recorded in all sampled months (FO 100%), while 46 were recorded in just one month (FO = 4%). About occurrence status, 55% of species are residents, 20% occasional visitors, 13% unusual residents, 6% migratory and 6% visitors. According the recorded species were recognized 25 trophic guilds based on food items, corporal size and strata that commonly forage, which of most representative in the community, the guild of “of insectivorous of medium-strata” (N = 55), “canopy omnivorous” (N = 33), “aquatic invertebrates consummers” and “piscivorous” (N = 31). Finally, with the current study, we aimed through a significative field effort bring a better knowledge about avifauna of PEIC, which could be a good base when is necessary take actions that aim to park management and the Conservation Unities around it.

A continent-wide high genetic load in African buffalo revealed by clines in the frequency of deleterious alleles, genetic hitchhiking and linkage disequilibrium

A high genetic load can negatively affect population viability and increase susceptibility to diseases and other environmental stressors. Prior microsatellite studies of two African buffalo (Syncerus caffer) populations in South Africa indicated substantial genome-wide genetic load due to high-frequency occurrence of deleterious alleles. The occurrence of these alleles, which negatively affect male body condition and bovine tuberculosis resistance, throughout most of the buffalo’s range were evaluated in this study. Using available microsatellite data (2–17 microsatellite loci) for 1676 animals from 34 localities (from 25°S to 5°N), we uncovered continent-wide frequency clines of microsatellite alleles associated with the aforementioned male traits. Frequencies decreased over a south-to-north latitude range (average per-locus Pearson r = -0.22). The frequency clines coincided with a multilocus-heterozygosity cline (adjusted R2 = 0.84), showing up to a 16% decrease in southern Africa compared to East Africa. Furthermore, continent-wide linkage disequilibrium (LD) at five linked locus pairs was detected, characterized by a high fraction of positive interlocus associations (0.66, 95% CI: 0.53, 0.77) between male-deleterious-trait-associated alleles. Our findings suggest continent-wide and genome-wide selection of male-deleterious alleles driven by an earlier observed sex-chromosomal meiotic drive system, resulting in frequency clines, reduced heterozygosity due to hitchhiking effects and extensive LD due to male-deleterious alleles co-occurring in haplotypes. The selection pressures involved must be high to prevent destruction of allele-frequency clines and haplotypes by LD decay. Since most buffalo populations are stable, these results indicate that natural mammal populations, depending on their genetic background, can withstand a high genetic load.

What the frequency list can teach us about Turkish sign language?

Recent studies on linguistics, cognitive science and psychology have shown that describing lexical frequency characteristics can answer many critical questions on language acquisition, mental lexicon and language use. Given the importance of corpus-based methodology, this study reports the preliminary findings from the objective lexical frequency list in TİD based on 103.087 sign tokens. This paper shows that frequency occurrence has a very decisive role on the linguistics categories and language in use. With respect to the multi-functionality of pointing in signed languages, the top ranked ID-gloss occurrences are mostly shaped by the pronominal references. Moreover, when compared to previous studies in terms of lexical density and lexical diversity, TİD shares both similar and different statistical features observed in other signed languages.

Diet Diversity and Dietary Overlap of Two Sympatric Vulture Species in Hirpora Wildlife Sanctuary, Kashmir

Bird species that use similar resources are expected to use variant foraging approaches and segregate in order to minimize a possible competition among them. However, if they fail to attain this exclusion, competition results. We studied the diet composition and dietary overlap between two sympatric vulture species: Himalayan vulture (Gyps himalayensis) and Bearded vulture (Gypaetus barbatus), the two important avian scavengers of Hirpora Wildlife Sanctuary of Kashmir Himalaya. Analysis of pellets collected from feeding and roosting sites of these vulture species revealed a significant variation in their food consumption with Himalayan species consuming mostly larger dead mammalian species with higher frequency occurrence of 66.66% of Bubalus bubalus and bearded species the smaller dead mammalian ones with highest frequency occurrence of Ovis aries (83.82%). Diet spectrum of Himalayan vulture was more diverse (H’=1.97) than that of Bearded vulture (H’=1.64). The values of Berger-Parker index (Pimax) follows the reverse order of diversity with Pimax=36.2% for Himalayan vulture and Pimax =48.7% for Bearded vulture. There was very low dietary overlap between the two species (Ojk=0.466; C=0.457) which may favor their co-existence. The management of carrions of livestock and setting up of vulture restaurants is the need of the hour for the conservation of these scavenging birds which are facing high risk of extinction in Hirpora Wildlife Sanctuary.

Thai Undergraduate Student Awareness of Regular and Irregular Grapheme-Phoneme Correspondence

<p>Recent events in Thailand in reference to the teaching of phonics for better comprehension of English vocabulary have highlighted the overuse of identifying letter-sound relationships in English by utilizing the familiar Thai orthography to assist developing Thai EFL learners. This paper investigated the long-term effects of using such pedagogy on recognizing regular and irregular Grapheme-Phoneme Correspondences (GPC) in English by Thai undergraduate students. To address this matter, the study used a convenience sampling of 373 first-year university students from 11 general education English classes at a mid-sized private university near Bangkok, Thailand. The familiar English poem <em>I Take It You Already Know</em> was employed for data collection, for it consists of a practical ratio of frequent and infrequent English grapheme-phoneme correspondences. Extensive lists of recognized grapheme-phoneme correspondences were used to identify the frequent or regular, and the infrequent or irregular main phoneme present in each of the 60 most frequently queried content words of the poem. Point-Biserial Correlation was employed to measure the strength of association between the frequency occurrence of the most queried content words from the data set, and the examined weighted word frequency data. The findings suggest that, in general, the Thai undergraduate students demonstrated an overall lack of recognition of regular and irregular Grapheme-Phoneme Correspondences of English. </p>

Frequency Occurrence and Percentage Distribution of Rh C, Rh c, Rh E and Rh e Blood Group Amongst Pregnant Women Attending Antenatal Clinic in Port Harcourt, Nigeria

Background: The Rhesus (Rh) blood group is one of the most complex blood groups in humans comprising mainly of Rh D, C, c, E and e. However, only Rh D is routinely screened for in Nigeria despite the fact that other Rh antigens are clinically significant and can cause haemolytic disease of the newborn and delayed haemolytic transfusion reactions. Aim: The aim was to determine the frequency distribution of Rh C, c, E and e blood groups among pregnant women attending antenatal clinic in Port Harcourt, Nigeria. Study Design: The study consisted of 147 apparently healthy pregnant women within the age range of 18-45 years, attending a selected Primary Healthcare Centre (Obio Cottage Hospital) in Port Harcourt. The study was carried out from January 20, 2020 to March 27, 2020. The presence of Rh C, c, E and e blood groups were investigated using Anti-C, c, E and e monoclonal antibody in the same order. Results: Rh C, c, E and e were observed in 38.09%, 100%, 17.68% and 100% in the same order. Conclusion: The study indicated dominance of Rh c and Rh e over Rh C and Rh E among the studied pregnant women. It is necessary to take into cognizance the fact that the presence of Rh C, c, E and e antigens may be the cause of some delayed transfusion reactions and haemolytic disease of the foetus and newborn. Therefore, routine antigen typing for Rh antigens may help in decreasing red blood cell allo-immunization and delayed haemolytic transfusion reaction during pregnancy.

Improving the Reversible LSB Matching Scheme Based on the Likelihood Re-encoding Strategy

In 2018, Tseng et al. proposed a dual-image reversible embedding method based on the modified Least Significant Bit matching (LSB matching) method. This method improved on the dual-image LSB matching method proposed by Lu et al. In Lu et al.’s scheme, there are seven situations that cannot be restored and need to be modified. Furthermore, the scheme uses two pixels to conceal four secret bits. The maximum modification of each pixel, in Lu et al.’s scheme, is two. To decrease the modification, Tseng et al. use one pixel to embed two secret bits and allow the maximum modification to decrease from two to one such that the image quality can be improved. This study enhances Tseng et al.’s method by re-encoding the modified rule table based on the probability of each hiding combination. The scheme analyzes the frequency occurrence of each combination and sets the lowest modified codes to the highest frequency case to significantly reduce the amount of modification. Experimental results show that better image quality is obtained using our method under the same amount of hiding payload.

High Frequency of Enterocytozoon bieneusi Genotype WL12 Occurrence among Immunocompromised Patients with Intestinal Microsporidiosis

Microsporidiosis is an emerging opportunistic infection causing severe digestive disorders in immunocompromised patients. The aim of this study was to investigate the prevalence of intestinal microsporidia carriage among immunocompromised patients hospitalized at a major hospital complex in the Tunis capital area, Tunisia (North Africa), and perform molecular epidemiology and population structure analyses of Enterocytozoon bieneusi, which is an emerging fungal pathogen. We screened 250 stool samples for the presence of intestinal microsporidia from 171 patients, including 81 organ transplant recipients, 73 Human Immunodeficiency Virus (HIV)-positive patients, and 17 patients with unspecified immunodeficiency. Using a nested PCR-based diagnostic approach for the detection of E. bieneusi and Encephalitozoon spp., we identified 18 microsporidia-positive patients out of 171 (10.5%), among which 17 were infected with E. bieneusi. Microsporidia-positive cases displayed chronic diarrhea (17 out of 18), which was associated more with HIV rather than with immunosuppression other than HIV (12 out of 73 versus 6 out of 98, respectively, p = 0.02) and correlated with extended hospital stays compared to microsporidia-negative cases (60 versus 19 days on average, respectively; p = 0.001). Strikingly, internal transcribed spacer (ITS)-based genotyping of E. bieneusi strains revealed high-frequency occurrence of ITS sequences that were identical (n = 10) or similar (with one single polymorphic site, n = 3) to rare genotype WL12. Minimum-spanning tree analyses segregated the 17 E. bieneusi infection cases into four distinct genotypic clusters and confirmed the high prevalence of genotype WL12 in our patient population. Phylogenetic analyses allowed the mapping of all 17 E. bieneusi strains to zoonotic group 1 (subgroups 1a and 1b/1c), indicating loose host specificity and raising public health concern. Our study suggests a probable common source of E. bieneusi genotype WL12 transmission and prompts the implementation of a wider epidemiological investigation.

RESEARCH OF THE INFLUENCE OF ANTIBACTERIAL AND FUNGICIDAL PREPARATIONS ON FORMATION BIOFILM OF MICROORGANISMS

The results of studies general patterns formation heterogeneous structure biofilms gram-negative and gram-positive bacteria, as well as yeast-like fungi Candida spp. are presented. Рrocesses intercellular communication of various systematic groups microorganisms has common morphological and functional patterns biofilm formation. Heteromorphic structures of biofilms united by the intercellular matrix have been revealed in natural, industrial, and clinical conditions, both in the body of mammals and birds, and in food products, devices and equipment, animal husbandry and food production technologies. Indication in a large number of microcolonies, as well as yeast and micellar phases in isolates from pathological material of animals, was a differential sign in local and systemic pathologies. Under the influence drugs on biofilms microorganisms, a direct correlation was established between morphometric and densitometric indicators, reflecting a decrease in the frequency occurrence clusters and optical density, respectively. Under the bacteriostatic effect of chemotherapeutic and disinfecting drugs, accumulations altered cells of spheroplastic type, capable forming stable and unstable L-forms, were revealed. For detection of viable microorganisms in a heterogeneous population microorganisms in vitro and in vivo, fluorescence microscopy and culture media with growth factors for the repair cell wall of L-forms bacteria are promising.

Species Composition and Biomass Production in Two Communal Coastal Rangelands of the Eastern Cape Province, South Africa

This study investigated species composition and biomass production of herbaceous plants in two communal rangelands surrounding homesteads areas. In each study area, the rangelands were divided based on the distance from homesteads into near (up to 1 km), middle (> 1–2 km) and far (> 2–3 km) sites. On each site a HVU of 50 m x 20 m was used and six 0.25 m2 quadrants were laid randomly on each HVU. Herbaceous species found within a quadrant were identified, counted and their height and tuft were measured. Herbaceous species were also harvested, bulked and placed into well labelled brown paper bags and oven-dried for 48 hours at 60 oC to determine biomass production. Herbaceous species were also classified according to their palatability ecological status and life form. Data were collected for two seasons over 2014/15 (winter and summer). A total of 20 herbaceous species were identified in the study areas. Of these 17 were grass species. The most common or dominant grass species were T. triandra, C. dactylon, E. capensis, E. plana and S. africanus. At Dyamdyam T. triandra showed the greatest frequency of occurrence at far site than middle and near homesteads sites. The frequency occurrence of S. africanus at Machibi was relatively similar in all the study sites. Density of herbaceous species was significantly lowest on near and bottom sites at Dyamdyam and Machibi, respectively. In both winter and summer, grazing site far and top from homestead had greatest (P <0.05) biomass production at Dyamdyam and Machibi respectively. The biomass production was significantly highest in the summer than the winter across the study sites in both study areas. It can be concluded that composition of herbaceous species and biomass production are significantly dependent on distance and topography from homesteads and seasons. Therefore, any rangeland management practices in communal grazing lands should consider these factors in to consideration during the planning of development progress.