Features of the Development and Application of Diagnostic ELISA Systems for the Detection of Antibodies to the SARS-CoV-2 Coronavirus in Clinical Practice

2021 ◽  
Vol 21 (1) ◽  
pp. 19-30
Author(s):  
Elena I. Zagoruyko ◽  
Dmitriy A. Kudlay ◽  
Maryana A. Lysenko ◽  
Darya S. Fomina ◽  
Ilya V. Vinogradov ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Scientific Literature ◽  
Disease Onset ◽  
Test System ◽  
Test Systems ◽  
Healthy Donors ◽  
National Production ◽  
Coronavirus Infection ◽  
Kinetics Of ◽  
Real Clinical Practice

Introduction. The new coronavirus infection, COVID-19, caused by SARS-CoV-2 has reached the scale of a pandemic. Detection of antibodies to SARS-CoV-2 is an aid in the diagnosis of COVID-19, and is also used to assess the immune response to the infection. Since COVID-19 is a new disease, seroconversions and kinetics of antibody to SARS-CoV-2 are not well understood. The aim of the study is to evaluate seroconversions and kinetics of antibodies to SARS-CoV-2 (IgA, IgM, IgG and total) in real clinical practice using test systems of national production. Materials and methods. 327 blood samples obtained from patients with a confirmed diagnosis of COVID-19 with active infection, convalescents and healthy donors were analyzed. Detection of antibodies to SARS-CoV-2 was carried out by enzyme immunoassay method using registered test systems Antigma-A, Antigma-G, Antigma-Screen and an unregistered test system for the detection of class M immunoglobulins. Results. When testing for total antibodies, 50.0% of samples were positive on days 1-7; 89.4% on days 8-14; 96.2% on days 15-45; and 93.3% after the 46th day after disease onset. The earliest seroconversions occurred on the 3rd day; antibodies were detected up to 81 days after the disease onset. Conclusions. The frequency of detecting seroconversions when using test systems of the Antigma series in real clinical practice is comparable to the data published in the world scientific literature and is high enough to use these test systems in the COVID-19 diagnostics.

The lung-gut axis and COVID-19

2021 ◽  
Vol 19 (1) ◽  
pp. 91-96
Author(s):  
V.P. Novikova ◽  
◽  
A.I. Khavkin ◽  
A.V. Polunina ◽  
A.V. Gorelov ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Gastrointestinal Tract ◽  
Scientific Literature ◽  
Intestinal Microbiome ◽  
Disease Course ◽  
Qualitative And Quantitative ◽  
Commensal Flora ◽  
Gastrointestinal Lesions ◽  
Coronavirus Infection ◽  
Quantitative Changes

This review summarizes relevant scientific literature analyzing the lung-gut axis and its association with coronavirus infection (COVID-19), lesions to the gastrointestinal tract caused by this infection, and state of the microbiome. Approximately 20%–50% of COVID-19 patients have such symptoms as nausea, vomiting, and diarrhea, as well as SARS-CoV-2 RNA detected in their feces. Therefore, investigation of the virus effect on the gastrointestinal tract and its commensal flora is important not only for research purposes, but for clinical practice, since patients with COVID-19 demonstrate both qualitative and quantitative changes in their microbiome. The latter may serve as a basis for the development of additional probiotic therapy for gastrointestinal lesions in COVID-19 patients. Despite the existing evidence, it is still necessary to clarify the effect of the intestinal microbiome on the pathogenesis of coronavirus infection and the disease course. Key words: COVID-19, coronavirus infection, microbiome, intestinal microbiota, SARS-CoV-2, probiotics, probiotic therapy

scholarly journals IFN-α as a time-sensitive biomarker during Oropouche virus infection in early and late seroconverters

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Euzébio de Oliveira ◽  
Raimunda do Socorro Silva Azevedo ◽  
Jordana Grazziela Coelho-dos-Reis ◽  
Lis Ribeiro do Valle Antonelli ◽  
Milene Silveira Ferreira ◽  
...  
Keyword(s):  
Virus Infection ◽  
Disease Onset ◽  
Healthy Donors ◽  
Ifn Γ ◽  
Kinetics Of

AbstractIn the present study, patients with acute OROV fever were classified as early seroconverters (IgM/IgG positive at baseline) or late seroconverters (IgM/IgG negative at baseline) and the timeline kinetics of the production of chemokines and cytokines were assessed at 1–3, 4–7, 8–10 and ≥11 days after patients have reported the first symptoms. Regardless immunoglobulin profile, all OROV fever patients presented higher levels of CXCL8, and IFN-α and lower levels of TNF and IL-10 at baseline as compared to healthy donors (HD). Lower levels of CCL2, CXCL10, and IFN-γ and higher levels of CCL2, CXCL10, IL-6, and IL-17A were detected in early and late seroconverters, respectively, as compared to HD. While early seroconverters presented the increasing levels of CCL2 along the timeline, late seroconverters displayed decreasing levels of CCL2, CXCL10, and IL-6 following days of disease onset. Noteworthy was that IFN-α was revealed as universal biomarker of human OROV fever, while CXCL8 & IL-5 and CXCL10 & IL-17 were consistently observed in early and late seroconverters, respectively. Thus, our results suggest that the production of IFN-α, CXCL10, and IL-17 precede the seroconversion bringing novel insights on the immunological events triggered by the OROV disease.

scholarly journals CORONAVIRUS PANDEMIC: EXPERIENCE OF THE FIRST WAVE IN THE NIZHNY NOVGOROD REGION

2021 ◽  
Vol 14 (2) ◽  
pp. 39-45
Author(s):  
VASILY D. FEDOTOV ◽  
◽  
ALEXANDER A. TULICHEV ◽  
OLGA O. FEDOTOVA ◽  
IRINA S. DOBROTINA ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Diagnosis And Treatment ◽  
Coronavirus Infection ◽  
Nizhny Novgorod Region ◽  
Real Clinical Practice

The aim of the study was to analyze the data from real clinical practice of diagnosis and treatment of a new coronavirus infection obtained during the first wave of the virus in Nizhny Novgorod Region.

In real clinical practice osteoporosis drugs are taken for a very short period: analysis of persistence in the campania region

2013 ◽  
Author(s):  
Giovanni Iolascon ◽  
Annarita Capaldo ◽  
Valentina Orlando ◽  
Enrica Menditto ◽  
Francesca Gimigliano
Keyword(s):  
Clinical Practice ◽  
Campania Region ◽  
Period Analysis ◽  
Short Period ◽  
Real Clinical Practice

PCSK9 inhibitors as an add on for the treatment of dislipemia in real clinical practice

2018 ◽  
Author(s):  
Carmen Hernandez Garcia ◽  
Cristina Maria Diaz Perdigones ◽  
Miguel Damas Fuentes ◽  
Clara Estaun Martinez ◽  
Andrea Sanchez Ramos ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Pcsk9 Inhibitors ◽  
Real Clinical Practice

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

Comprehensive assessment of drug consumption as a basis for pharmacotherapy optimization

2019 ◽  
pp. 22-31
Author(s):  
Olga Vyacheslavovna Zhukova
Keyword(s):  
Clinical Practice ◽  
Treatment Options ◽  
Comprehensive Assessment ◽  
Drug Consumption ◽  
Treatment Standards ◽  
Disease Epidemiology ◽  
Efficacy Analysis ◽  
Epidemiological Monitoring ◽  
Guidelines Development ◽  
Real Clinical Practice

This article describes the rationale for methodology of comprehensive assessment of drug consumption in real clinical practice. The proposed methodology includes three stages: 1) epidemiological monitoring – disease epidemiology assessment; assessment of the role of factors leading to the disease; 2) pharmacoepidemiological monitoring – assessment of pharmacotherapy in real clinical practice; clinical efficacy analysis of drugs; cost-effectiveness analysis; 3) long-term clinical and economical evaluation of various treatment options. Comprehensive assessment of drug consumption should result in optimal pharmacotherapy regimens, decrease of the drug load, increasing of the therapy effectiveness and cost reduction. The scheme of comprehensive assessment of drug consumption is universal and can be used for clinical guidelines development, treatment standards, for the optimal formation of drugs lists at the federal level. Separate stages and sub-steps of an integrated assessment also can be used at the territorial and local levels, medical institution, to optimize pharmacotherapy.

Pharmacoepidemiological Analysis of Pharmacotherapy for Burn Injuries in Real Clinical Practice

2020 ◽  
pp. 70-79
Author(s):  
Olga Vyacheslavovna Zhukova ◽  
Ekaterina Sergeevna Nekaeva ◽  
Elena Sergeevna Khoroshavina ◽  
Ekaterina Alexeevna Kozlova ◽  
Yulia Alexandrovna Dudukina ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Average Cost ◽  
Burn Injury ◽  
General Structure ◽  
Burn Injuries ◽  
Antimicrobial Drugs ◽  
Positive Tendency ◽  
Defined Daily Doses ◽  
Duration Of Hospitalization ◽  
Real Clinical Practice

Objective: to conduct pharmacoepidemiological analysis and analysis of the costs of pharmacotherapy, taking into account the actual consumption of drugs in the real inpatient clinical practice at the federal center in Russia. Materials and methods. Data from the medical records of 14 patients with burn injury, who were hospitalized in 2018, was analyzed. Patients’ age was from 23 to 67 years (44,93 ± 14,66). Duration of hospitalization was from 17 to 62 days (35,93 ± 14,17). We calculated rate of prescription foe each drug and its share in general structure of all utilized drug courses (n = 460). We performed frequency analysis of prescription structure, DDD (defined daily doses) analysis, DU90% (Drug Utilization 90%) analysis, ABC-analysis and analysis of average cost of pharmacotherapy. Results. Most frequently used drugs, prescribed in 75-100% of all hospital cases, included 15 names, e.g. 2 antimicrobial drugs (vancomycin and amikacin), 19 were used commonly, including 4 antimicrobial drugs (co-trimoxazole, cefoperazone/sulbactam, tigecyclin and cefepime). Other drugs were used in less than 25% of cases. 33 drugs made 90% of all consumed NDDD, including 5 antimicrobial drugs (vancomycin, amikacin, co-trimoxazole, cefoperazone/sulbactam and tigecyclin). These drugs comprised 70,24% in the prescription structure. The cost of one DDD in DU90% segments (512,33 rubles) is 1,4 higher than in DU10% segment (649,34 rubles). Average cost of drugs included in DU90% group was 4735,89 rubles vs 4966,80 rubles for drugs from DU10% group. This finding shows positive tendency of burn injuries pharmacological treatment. Conclusion. We obtained the data, which can be used for comparison of real clinical practice costs with a current payment rates for medical care.

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