scholarly journals Experimental Modelling of the Processes for Connective Tissue Reparative Regeneration under Unfavorable Conditions

2015 ◽  
Vol 22 (1) ◽  
pp. 18-22
Author(s):  
M. V Vlasov ◽  
S. N Bugrov ◽  
A. B Bogos’yan ◽  
I. V Musukhina
Keyword(s):  
Connective Tissue ◽  
Fibrous Tissue ◽  
Reparative Regeneration ◽  
Lymphoid Cells ◽  
Fatty Tissue ◽  
Experimental Modelling ◽  
Defect Zone ◽  
Dense Fibrous Tissue ◽  
Mesenteric Vessels ◽  
Reverse Development

The purpose of the work was to elaborate experimental model for transverse tenotomy with intersection of facial membranes and mesenteric vessels as well as to study the process of connective tissue reparative regeneration under created conditions. Experiment was performed on 25 chinchilla rabbits of both sexes, aged 1 month with weight 1376 ± 124.8 g. Processes of Achilles tendon reparative regeneration was studied on days 15, 21, 30, 60 and 90 after operation. Histomorphologic study showed that within 30 days after tenotomy the substitution of defect zone with friable, reach in cells and gradually maturating fibrous tissue took place. By the end of day 60 a considerable part of the defect was substituted by dense fibrous connective tissue assumed a scar pattern. On day 90 after tenotomy a reverse development of the defect zone with focal substitution of dense fibrous tissue by friable unformed connective tissue containing histiocytes, lymphoid cells and inclusions of fatty tissue foci was observed. At that term histologic structure of the specimen showed its relatively low mechanical strength. The achieved data enabled to demonstrate that performance of tenotomy with traumatization of peritendinous tissues adversely affected the dynamics and pattern of reparative processes in the tendinosus defect zone.

scholarly journals Experimental Modelling of the Processes for Connective Tissue Reparative Regeneration under Unfavorable Conditions

2015 ◽  
pp. 18-22
Author(s):  
M. V. Vlasov ◽  
S. N. Bugrov ◽  
A. B. Bogos’Yan ◽  
I. V. Musukhina
Keyword(s):  
Connective Tissue ◽  
Fibrous Tissue ◽  
Reparative Regeneration ◽  
Lymphoid Cells ◽  
Fatty Tissue ◽  
Experimental Modelling ◽  
Defect Zone ◽  
Dense Fibrous Tissue ◽  
Mesenteric Vessels ◽  
Reverse Development

The purpose of the work was to elaborate experimental model for transverse tenotomy with intersection of facial membranes and mesenteric vessels as well as to study the process of connective tissue reparative regeneration under created conditions. Experiment was performed on 25 chinchilla rabbits of both sexes, aged 1 month with weight 1376 ± 124.8 g. Processes of Achilles tendon reparative regeneration was studied on days 15, 21, 30, 60 and 90 after operation. Histomorphologic study showed that within 30 days after tenotomy the substitution of defect zone with friable, reach in cells and gradually maturating fibrous tissue took place. By the end of day 60 a considerable part of the defect was substituted by dense fibrous connective tissue assumed a scar pattern. On day 90 after tenotomy a reverse development of the defect zone with focal substitution of dense fibrous tissue by friable unformed connective tissue containing histiocytes, lymphoid cells and inclusions of fatty tissue foci was observed. At that term histologic structure of the specimen showed its relatively low mechanical strength. The achieved data enabled to demonstrate that performance of tenotomy with traumatization of peritendinous tissues adversely affected the dynamics and pattern of reparative processes in the tendinosus defect zone.

scholarly journals MORPHOLOGICAL FEATURES OF REGENERATION OF THE ACHILLES TENDON AFTER ITS CROSS TENOTOMY WITH INTERSECTION OF FASCIAL SHEATHS AND DAMAGE OF MESENTERIC VESSELS

2016 ◽  
Vol 175 (1) ◽  
pp. 59-63
Author(s):  
M. V. Vlasov ◽  
S. N. Bugrov ◽  
A. B. Bogos’Yan ◽  
I. V. Musikhina
Keyword(s):  
Connective Tissue ◽  
Achilles Tendon ◽  
Fibrous Tissue ◽  
Complete Recovery ◽  
Reparative Regeneration ◽  
Morphological Features ◽  
Lymphoid Cells ◽  
Fatty Tissue ◽  
Circulatory Disturbance ◽  
Mesenteric Vessels

The article is aimed to study morphological features of regeneration process of the Achilles tendon after its cross tenotomy with intersection of facial sheaths and damage of mesenteric vessels in experiment on growing rabbits. The experiment was made on 25 rabbits of «Shinshilla» breed of both sexes in the age of one month with the mass of 1376±124,8 g. The processes of reparative regeneration of heel tendon were investigated. There was used a rabbit’s output from the experiment on 15, 21,30,60 and 90 days after cross tenotomy in number of five animal units. According to results of carried out researches, the considerable part of defect was completed by dense connective tissue obtaining scarry character on 60 days. There was observed an opposite development of defect zone, a focal substitution of dense fibrous tissue by loose connective tissue with histiocytic, lymphoid cells and inclusions of foci of fatty tissue on 90 days. The authors didn’t noted the complete recovery of tendinous tissues «retitucica ad integrum» in the zone of tenotomy, because of circulatory disturbance in this area due to damage of facial sheats and mesenteric vessels. Thereby, the reparative regeneration of heel tendon was originated in unfavorable conditions, because of cross tenotomy performance with intersection of synovial sheath and damage of mesenteric vessels. The results obtained indicated that it is necessary to avoid the damage of synovial sheathes and mesenteric vessels in cross intersection of heel tendon during correction of foot element deformations.

Hyperplasia of the bile duct in fascioliasis and its relation to the problem of nutrition in the liver-fluke, Fasciola hepatica L.

Parasitology ◽  
1963 ◽  
Vol 53 (1-2) ◽  
pp. 123-133 ◽  
Author(s):  
Ben Dawes
Keyword(s):  
Bile Duct ◽  
Connective Tissue ◽  
Bile Ducts ◽  
Inflammatory Process ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Fibrous Tissue ◽  
Feeding Habit ◽  
Glandular Tissue ◽  
Dense Fibrous Tissue

Histological investigations of the bile ducts of various hosts of F. hepatica during the early stages of infection, when the flukes are burrowing in the liver and have not yet reached the bile ducts, indicate that subtle changes are brought about, probably by chemical agencies and as a result of the inflammatory process, in the bile ducts of the hosts. The transitory appearance of minute cytoplasmic blebs at the free margins of the epithelial cells seems to inaugurate an intense hyperplasia of the epithelium with tubular ingrowths and adenomatous development of glandular tissue, and an associated fibrosis which produces a thick underlying layer of dense fibrous tissue. At the time of entry of the flukes into the biliary system the hyperplastic epithelium is intact, but soon the spines and suckers of the flukes abrade the superficial cells until this part of the duct is completely denuded. It is suggested that as a result of the inflammatory reaction, the fluke has been provided with a ‘pasture’ of hyperplastic epithelium and connective tissue on which it may continue to browse for considerable periods without necessarily affecting the host adversely. Tissue debris is found in the contents of the bile duct and similar homogenized materials occur in the caecal contents of the fluke. Previous researches have shown that the fluke will feed on various tissues, and it is suggested that this tissue-feeding habit persists during its life in the bile duct. These results agree with the findings of Müller (1923) but are at variance with the results obtained by other investigators, as is shown in discussion. The hyperplasia of the bile duct is not characteristic of fascioliasis, occurring also in clonorchiasis, opisthorchiasis and dicrocoeliasis, and in these and possibly other instances it is probably intimately connected with the nutrition of the flukes concerned.

The cardiac conduction system in Hurler syndrome: pathological features and clinical implications

1992 ◽  
Vol 2 (2) ◽  
pp. 196-199
Author(s):  
Louis Tsun-cheung Chow ◽  
Wing-Hing Chow
Keyword(s):  
Sinus Node ◽  
Fibrous Tissue ◽  
Conduction System ◽  
Cardiac Conduction ◽  
Cardiac Conduction System ◽  
Clinical Implications ◽  
Hurler Syndrome ◽  
Pathological Features ◽  
Hydropic Degeneration ◽  
Dense Fibrous Tissue

SummaryWe studied the cardiac conduction system in a case of Hurler syndrome. There was dense fibrosis of the supporting matrix of the sinus node and accumulation of mucopolysaccharide in the nodal cells. The bundle branches showed prominent hydropic degeneration, being encased and punctuated by dense fibrous tissue. These changes in the conduction system may predispose to the development of arrhythmias, accounting for the sudden deaths in Hurler syndrome.

Porudominskie and Herzenberg. Towards the doctrine of the pathological anatomy of male gonorrhea. (Ztsch. F. Urol, No. II, 1936)

1937 ◽  
Vol 33 (7) ◽  
pp. 938-938
Keyword(s):  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Squamous Epithelium ◽  
Plasma Cells ◽  
Lymphoid Cells ◽  
Genitourinary Tract ◽  
Columnar Epithelium ◽  
Pathological Anatomy ◽  
Surface Layers

The material of the authors consists of 5 cases of gonorrhea that died from accidental causes, 1 case of amputation and 3 cases. extirpation of the paraurethral course. Conclusions: the gonorrhea process is played out in the epithelium and connective tissue of the genitourinary tract and has an exudative-proliferative character. The inflammatory infiltrate consists of lymphoid cells, plasma cells, leukocytes, and eosinophils. The squamous epithelium is not an obstacle to the penetration of infection this is clearly seen when studying the process in the paraurethral passages. The penetrated deep into the gonococcus are phagocytosed by lecocytes in the surface layers of the submucosa. Metaplasia of columnar epithelium in squamous and transitional, can be in any part of the genitourinary tract and can be detected before the onset of gonorrhea. Litreitis is not necessary for gonorrhea. Prostatitis can be without vesiculitis.

scholarly journals The neuroma-like structure of the long-time severed and isolated nerve stumps

1965 ◽  
Vol 23 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Eros Abrantes Erhart ◽  
Cecil J. Rezze
Keyword(s):  
Connective Tissue ◽  
Fibrous Tissue ◽  
Nerve Fibres ◽  
Present Complex ◽  
Proximal Stump ◽  
Long Time ◽  
Distal Nerve

The long-time severed and isolated intermediate and distal nerve segments, maintained undisturbed in their connective tissue bed, completely separated from each other and from the proximal stump, are repopulated by nerve-fibres which origin is still unknown. The extremities of such nerve segments present complex nervous nodules capped by fibrous tissue, neuroma-like structures.

scholarly journals THE ORIGIN AND STRUCTURE OF A FIBROUS TISSUE WHICH APPEARS IN LIVING CULTURES OF ADULT FROG TISSUES

1915 ◽  
Vol 21 (5) ◽  
pp. 455-478 ◽  
Author(s):  
George A. Baitsell
Keyword(s):  
Wound Healing ◽  
Connective Tissue ◽  
Fibrous Tissue ◽  
Cell Movement ◽  
Isolated Cells ◽  
Plasma Clot ◽  
True Nature ◽  
Adult Frog ◽  
Hanging Drop Method ◽  
Living Tissues

In living cultures of various kinds of adult frog tissues, which have been made according to the hanging drop method, there occurs, in many cases, a transformation of the plasma clot by which it becomes entirely changed from a typical fibrin net both in appearance and structure. The changes in the fibrin net generally begin when the culture is from two to three days old. During these changes it appears that the elements of the fibrin net fuse or consolidate, and as a result a great number of fine wavy fibrils are formed which unite to form wavy bundles of fibers, and these freely intertwine and anastomose as they ramify through the area of the plasma clot. The transformation of the fibrin net occurs first in the region of the clot which lies next to the imbedded tissue, gradually extends to the distal regions of the clot, and in time—as a rule in about two weeks—the entire plasma clot becomes changed from the fibrin net into a structure which to all appearances is identical with regular connective tissue. Photographs of both living and preserved cultures have been made to show the course of the transformation of the plasma clot and the development of the fibers. Experiments have been made which show that the fibers which are formed are not outgrowths of the imbedded tissue. Also they are not formed by an intracellular action, but arise directly by a transformation of the fibrin elements of the plasma clot. Experiments have been made which indicate that the transformation of the fibrin net will not occur unless it has come under the influence of living tissues or of living isolated cells. However, mechanical means, such as exerting tension on the clot with needles, may hasten the formation of the fibers. Also, in some cultures, movements of living isolated cells appear to aid in the formation of the fibers. The living tissues alone, however, are able to cause the fibers to form without the aid of any apparent mechanical factor. This is shown by cultures of various tissues in which no cell movement occurs and in which the plasma clot is undisturbed and yet a prolific formation of fibers may take place. Experiments have been made in order to determine the true nature of the transformed plasma clot and to see if the new fibrous tissue were still fibrin in character. The results that have so far been obtained from these tests have not been entirely conclusive and leave the question unsettled. The transformation of the fibrin net results in a shrinkage of the clot. It also becomes very tough and resistant to injury and, therefore, entirely different from the fragile and easily destroyed fibrin net when in its original condition. It is believed that such a reaction must play an important part in wound healing. A study of the relation between connective tissue fibers formed in wound healing and in embryonic development to the fibers formed in the plasma clot is being made, and the results will appear in a later paper.

Magnetic resonance imaging after frontal sinus surgery with fat obliteration

1995 ◽  
Vol 109 (11) ◽  
pp. 1115-1119 ◽  
Author(s):  
R. Keerl ◽  
R. Weber ◽  
G. Kahle ◽  
W. Draf ◽  
J. Constantinidis ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Connective Tissue ◽  
Frontal Sinus ◽  
Spin Echo ◽  
Sinus Surgery ◽  
Fast Spin Echo ◽  
Fatty Tissue ◽  
Resonance Imaging ◽  
Magnetic Resonance Imaging Mri

AbstractThe obliteration of the frontal sinus via an osteoplastic approach is performed with the aim of achieving a permanent ‘switching off’ by final and conclusive clearing out. For this, freshly harvested abdominal fat has shown itself to be the best clinically. It is possible to demonstrate the vitality of fat transplanted into the frontal sinus without an operation i.e. by a macroscopical and histological examination using magnetic resonance imaging (MRI). The magnetic resonance examinations were carried out on a supraconductive 0.5 T Magnet (Gyroscan T.S.II, Philips Medicine Systems, Eindhoven, Netherlands) with a quadrature (square) head spool. We produced T1-weighted spin echo images (TR: 450–550 ms; TE: 20–25 ms), T2-weighted fast spin echo images or in double-echo technique in transverse orientation (Turbo SE or TR: 2000–2500 ms; TE: 50–90 ms) and short tau inversion recovery (STIR) sequences for fat suppression (TJ: 140 ms; TR: 1400 ms; TE: 30 ms). The fat implanted into the frontal sinus of 11 patients aged 22–65 years, having undergone an osteoplastic frontal sinus operation with obliteration, was examined post-operatively by MRI. Objectives were the time-dependent distribution of portions of vital fatty or connective tissue, the eventual development of necroses or cysts as well as recurrences, inflammatory complications or re-epithelization of the frontal sinus four to 24 months postoperatively. In only six out of 11 cases was vital fatty tissue found. Fatty necrosis occurred five times, whereas in four cases a transformation into granulation tissue and in one case into connective tissue could be seen. All 11 patients were complaint-free. Long-term observations are needed to see if differences in the recurrence rate of frontal sinus disease are dependent on whether the implanted fat remains vital or necrosed and transformed.

OBSERVATIONS ON THE HISTOPATHOLOGICAL CHANGES CAUSED BY THE LARVAE OF HYPODERMA BOVIS (L.) AND HYPODERMA LINEATUM (DE VILL.) (DIPTERA: OESTRIDAE) IN TISSUES OF CATTLE

1959 ◽  
Vol 39 (2) ◽  
pp. 145-157 ◽  
Author(s):  
L. S. Wolfe
Keyword(s):  
Connective Tissue ◽  
Plasma Cells ◽  
Fibrous Tissue ◽  
Ground Substance ◽  
Collagen Fibres ◽  
Hypoderma Lineatum ◽  
Cellular Debris ◽  
Hypoderma Bovis ◽  
Epidural Fat ◽  
Suppurative Inflammation

The lesions in submucosal and adventitial connective tissue of the oesophagus of cattle caused by the migrating larvae of Hypoderma lineatum (De Vill.), consist of pseudocystic areas and tracks of a gelatinous fibrinous exudate containing necrotic cellular debris and collagen fibres in various stages of dissolution surrounded by accumulations of eosinophils and lymphocytes. The larvae feed on the dissolved connective tissue. These changes suggest that migration through the tissues is mainly by enzymic dissolution of collagen fibres and ground substance. Similar changes were found in the connective tissue surrounding the muscles of "licked beef". The larvae of H. bovis (L.) in the epidural fat cause areas of fat necrosis and sometimes invade the periosteum and dura. The greenish colour of the tracks is due to infiltrations of eosinophils. The warble shows a mixed picture of chronic granulomatous and non-suppurative inflammation, encapsulated by dense fibrous tissue. The larva is surrounded by a vascular layer of granulation tissue infiltrated with eosinophils, large mononuclears and plasma cells. Numerous giant cell systems and pseudotubercles are present. The larvae feed on the exudate within the warble cavity. Areas of suppuration are rarely found. The healing of the warble and the reaction to dead larvae are described.

scholarly journals A HISTOLOGICAL STUDY OF THE SKIN LESIONS OF PELLAGRA

1911 ◽  
Vol 13 (1) ◽  
pp. 98-114
Author(s):  
Fraser B. Gurd
Keyword(s):  
Epithelial Cells ◽  
Connective Tissue ◽  
Blood Vessels ◽  
Pathological Condition ◽  
Histological Study ◽  
The Body ◽  
Lymphoid Cells ◽  
Cutaneous Lesions ◽  
Predisposing Factor ◽  
Pigment Formation

The cutaneous lesions in pellagra consist of an early erythema, or, in occasional cases, of vesicles or bullous formations which are followed by hyperkeratosis and pigmentation, resulting in a dry, dark brown scaliness. These various lesions are similar to those normally produced by the action of sunlight, but are much more marked. The histological phenomena of the erythematous and bullous stage are those of a mild acute inflammatory reaction, together with a degeneration in the superficial layers of the corium. Following this degeneration, which involves not only the general connective tissue but the connective tissue of the blood vessels, there is a reparative change evidenced histologically by an increased cellularity of the corium and the presence of fibroblasts. The capillaries also are increased in number and much dilated. Apparently as a result of this increased vascularity of the corium, there is an increased proliferation of the epithelium resulting in a thickening of the epidermis. This increase in thickness of the epithelial layer is especially marked in the prickle cells and the stratum granulosum. In the later stages, in an effort to secure a firm basement membrane, the epithelium is seen to dip down deeply into the rarefied connective tissue. About the blood vessels during the reactionary process are found collections of lymphoid cells, a few plasma cells, but no mast cells or eosinophiles. That the irritant producing the degeneration in the corium is sunlight in the presence of some predisposing factor, is suggested by the enormous increase in pigment formation in the epithelial cells and by the large number of chromatophores in the superficial layers of the corium. This pigmentation is autochthonous in both types of cell. There is no reason for believing that the pigment is formed in the cells of the corium and thence discharged into the epithelium, or that the reverse process takes place. The predisposing factor inducing the changes in the corium is, apparently, a lessened resistance of the epithelium to the violet and ultra-violet rays, due to some metabolic insufficiency on the part of the epithelial cells. Further observation may justify the conclusion that throughout the body, pellagra is a disease essentially of the epithelium, including the nervous system, this pathological condition manifesting itself by an insufficient or altered function.

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