scholarly journals Chemical Fingerprint Analysis and Quantitative Analysis of Saccharides in Morindae Officinalis Radix by HPLC-ELSD

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7242
Author(s):  
Hongmei Sun ◽  
Yini Cai ◽  
Jie Shen ◽  
Enyao Ma ◽  
Zhimin Zhao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Ion Mobility ◽  
High Performance ◽  
Gradient Elution ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

A method based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the quantitative analysis of three active compounds and chemical fingerprint analyses of saccharides in Morindae officinalis radix. Ten batches of Morindae officinalis radix were collected from different plantations in the Guangdong region of China and used to establish the fingerprint. The samples were separated with a COSMOIL Sugar-D column (4.6 mm × 250 mm, 5 μm) by using gradient elution with water (A) and acetonitrile (B). In addition, Trapped-Ion-Mobility (tims) Time-Of-Flight (tims TOF) was used to identify saccharides of Morindae officinalis radix. Fingerprint chromatogram presented 26 common characteristic peaks in the roots of Morinda officinalis How, and the similarities were more than 0.926. In quantitative analysis, the three compounds showed good regression (r = 0.9995–0.9998) within the test ranges, and the recoveries of the method were in the range of 96.7–101.7%. The contents of sucrose, kestose and nystose in all samples were determined as 1.21–7.92%, 1.02–3.37%, and 2.38–6.55%, respectively. The developed HPLC fingerprint method is reliable and was validated for the quality control and identification of Morindae officinalis radix and can be successfully used to assess the quality of Morindae officinalis radix.

scholarly journals A Strategy for Quality Control of Vespa magnifica (Smith) Venom Based on HPLC Fingerprint Analysis and Multi-Component Separation Combined with Quantitative Analysis

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2920 ◽  
Author(s):  
Si-Tong Zhou ◽  
Kai Luan ◽  
Lian-Li Ni ◽  
Ying Wang ◽  
Shi-Meng Yuan ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Principal Component ◽  
Good Precision ◽  
Fingerprint Analysis ◽  
Wasp Venom ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Relative Standard

As a folk medicine of the Jingpo minority in Yunnan province, the venom of Vespa magnifica has been commonly used for the treatment of rheumatoid arthritis. Quality standardization of the wasp venom is a necessary step for its pharmaceutical research and development. To control the quality of the wasp venom, a method based on high-performance liquid chromatography (HPLC) was developed for chemical fingerprint analysis. In the chromatographic fingerprinting, chemometrics procedures, including similarity analysis (SA), hierarchical clustering analysis (HCA), and principal component analysis (PCA), were applied to classify 134 batches (S1–S134) of wasp venom from different origins. The HPLC fingerprint method displayed good precision (Relative standard deviation, RSD < 0.27%), stability (in 16 h, RSD < 0.34%), and repeatability (RSD < 1.00%). Simultaneously, four compounds (VMS1, VMS2, VMS3, and VMS4) in the wasp venom were purified and identified. VMS1 was 5-hydroxytryptamine, and the other compounds were three peptides that were sequenced as follows: Gly–Arg–Pro–Hyp–Gly–Phe–Ser–Pro–Phe–Arg–Ile–Asp–NH2 (VMS2), Ile–Asn–Leu–Lys–Ala–Ile–Ala–Ala–Leu–Ala–Lys–Lys–Leu–Leu–NH2 (VMS3), and Phe–Leu–Pro–Ile–Ile–Gly–Lys–Leu–Leu–Ser–Gly–Leu–Leu–NH2 (VMS4). The quantifications for these components were 110.2 mg/g, 26.9 mg/g, 216.3 mg/g, and 58.0 mg/g, respectively. The results of this work indicated that the combination of the chemical fingerprint and quantitative analysis offers a reasonable way to evaluate the quality of wasp venom.

scholarly journals Identification and Quantification of three Tubeimosides in Rhizoma Bolbostematis by High Performance Liquid Chromatography with Evaporative Light Scattering Detection and Electrospray Mass Spectrometric Detection

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Hao Huang ◽  
MingJin Liang ◽  
Wen Xu ◽  
Chuan Zhang ◽  
WeiDong Zhang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Light Scattering ◽  
High Performance ◽  
Methanol Extract ◽  
Gradient Elution ◽  
Evaporative Light Scattering Detection ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

Tubeimoside I, tubeimoside II and tubeimoside III were simultaneously determined and identified by high performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-ESI/MS), and a novel and sensitive high performance liquid chromatography - evaporative light scattering detection (HPLC-ELSD) in a 70% methanol extract of Rhizoma Bolbostematis. The chromatographic separation was performed on a Zorbax Extend C18 analytical column using gradient elution with a solution of acetonitrile and 0.5% acetic acid. The method was validated with acceptable linearities (r > 0.9992) and recoveries (98.6 to 102.4 %). The limits of detection of these three tubeimosides were as low as 0.05 μg. The intra- and inter-day precisions of the method were evaluated and were less than 3.6%. The method was successfully used to analyze 15 batches of Rhizoma Bolbostematis. The content of tubeimosides in the plant material varied from habitat to habitat confirming the necessity to control the quality of Rhizoma Bolbostematis during its preparation and application in the clinic.

scholarly journals Determination of Triterpenoids and Phenolic Acids from Sanguisorba officinalis L. by HPLC-ELSD and Its Application

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4505
Author(s):  
Jiahui Sun ◽  
Chunli Gan ◽  
Jing Huang ◽  
Zhenyue Wang ◽  
Chengcui Wu ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Gradient Elution ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Acetic Acid Water ◽  
Sanguisorba Officinalis ◽  
Functional Components ◽  
Gradient Elution Mode

A novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for simultaneous determination of 11 phenolic acids and 12 triterpenes in Sanguisorba officinalis L. Chromatographic separation was conducted with gradient elution mode by using a DiamonsilTM C18 column (250 mm × 4.6 mm, 5 μm) with the mobile phase of 0.1% acetic acid water (A) and methanol (B). The drift tube temperature of ELSD was set at 70 °C and the nitrogen cumulative flow rate was 1.6 L/min. The method was fully validated to be linear over a wide concentration range (R2 ≥ 0.9991). The precisions (RSD) were less than 3.0% and the recoveries were between 97.7% and 101.4% for all compounds. The results indicated that this method is accurate and effective for the determination of 23 functional components in Sanguisorba officinalis L. and could also be successfully applied to study the influence of processing method on those functional components in Sanguisorba officinalis L.

scholarly journals Quality Evaluation of Fritillaria thunbergii by High-Performance Liquid Chromatography Fingerprinting Coupled with Multicomponent Quantitative Analysis

2021 ◽  
Vol 25 (01) ◽  
pp. 131-138
Author(s):  
Ruoxu He
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Quality Evaluation ◽  
Light Scattering Detection ◽  
Mobile Phases ◽  
Evaporative Light Scattering ◽  
Significant Difference ◽  
The Family ◽  
Main Production ◽  
Main Components

Medicinal Zhebeimu refers to the dried bulb of the plant Fritillaria thunbergii, which belongs to the family Liliaceae. Their main production sites are in Zhejiang, Jiangsu, and Anhui Provinces, with alkaloids as the main components. In this study, according to Chinese Pharmacopoeia, different mobile phases were investigated. Because most alkaloids in Fritillaria do not have UV absorption, evaporative light scattering detection (ELSD) was used instead of UV to produce the HPLC-ELSD spectra for five F. thunbergii samples and other samples, e.g. F. chuanxiensis, F. anhuiensis, and F. ussuriensis. Comparison showed significant differences exist among these species. There was no significant difference between the F. thunbergii samples produced in Zhejiang, which clustered together in both the cluster analyses. However, the F. thunbergii samples produced in Nantong, Jiangsu and the F. thunbergii samples produced in Zhejiang were separated in the clustering chart, which may be caused by different cultivation environments. The obtained results showed that the combination of quantitative analysis and chromatographic fingerprint analysis can be readily utilized for quality control purposes, offering a comprehensive strategy for quality evaluation of F. thunbergii and related products. © 2021 Friends Science Publishers

scholarly journals Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of Platycodi Radix Collected from Various Regions in China by HPLC Coupled with Chemometrics

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1823 ◽  
Author(s):  
Haiyang Lu ◽  
Mengzhen Ju ◽  
Shanshan Chu ◽  
Tao Xu ◽  
Yuzhe Huang ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Southern China ◽  
Pulmonary Diseases ◽  
Evaporative Light Scattering Detection ◽  
Fingerprint Analysis ◽  
Light Scattering Detection ◽  
Platycodi Radix ◽  
Evaporative Light Scattering ◽  
Long Time

Platycodi Radix (PR) is the root of Platycodon grandiflorum (Jacq.) A. DC., which has been used for a long time in China to treat pulmonary diseases. The present study aimed to evaluate the quality of PR samples collected from 23 regions of 11 provinces in China. Eight saponins were quantified using HPLC coupled with evaporative light scattering detection (HPLC-ELSD). The samples with the highest total contents of saponins were from southern China, such as Yunnan, Guangxi, Jiangxi, and Guangzhou. The fingerprint analysis of PR samples was conducted by HPLC-UV method. Nineteen common peaks were selected and the similarity values varied from 0.607 to 0.921. These findings indicated that the saponins contents of PR from different regions varied significantly, with PR samples from southern China having the highest contents of saponins. These comprehensive methods were successful in evaluating the quality of PR samples from northern and southern China, which will serve as a guide for the development of PR as a clinical medication.

Free Amino Acids Fingerprint Analysis for the Quality Evaluation of Oriental Latrine Fly Larvina by RP-HPLC Pre-Column Derivatization Method

2013 ◽  
Vol 781-784 ◽  
pp. 1842-1846
Author(s):  
Yu Ping Wu ◽  
Fu Jiang Chu ◽  
Xiao Bao Jin ◽  
Wen Bin Liu ◽  
Yin Ye Xu ◽  
...  
Keyword(s):  
Free Amino ◽  
High Performance ◽  
Quality Evaluation ◽  
Control Method ◽  
Gradient Elution ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint ◽  
Rp Hplc ◽  
Quality Control Method

A simple and reliable method of high-performance liquid chromatography with pre-column derivatization was developed to evaluate the quality of Oriental Latrine Fly Larvina through establishing chromatographic fingerprint. The separation was performed on a welch Ultimate XB-C8 column with a liner gradient elution of acetonitrile and acetate buffer. The developed method was validated by precision, stability and recovery. This study indicated that the chromatographic fingerprint analysis can be readily utilized as a quality control method for oriental latrine fly larvina.

Fingerprint Analysis of Flos Lonicerae by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

2012 ◽  
Vol 554-556 ◽  
pp. 2058-2063
Author(s):  
Feng Lai Lu ◽  
Chuan Ming Fu ◽  
Hai Ying Jiang ◽  
Yue Yuan Chen ◽  
Jin Lei Liu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Flow Rate ◽  
Retention Time ◽  
High Performance ◽  
Evaporative Light Scattering Detection ◽  
Fingerprint Analysis ◽  
Light Scattering Detection ◽  
Flos Lonicerae ◽  
Evaporative Light Scattering ◽  
Relative Standard

A high-performance liquid chromatographic method (HPLC) with evaporative light scattering detection (ELSD) has been developed for fingerprint analysis of Flos Lonicerae. The samples were separated with an Agilent C18 column using acetonitrile (A) and 0.1% formic acid solution under gradient conditions (0→10 min：11.5%→15% A, 10→12 min：15% →22% A, 12→18min：22%→33% A; 18→30min: 33%→45% A) as the mobile phase at a flow rate of 0.8ml min−1 within 30 min. The ELSD conditions were optimized at nebulizer-gas flow rate 2.7 L min−1 and drift tube temperature 105°C. Precision experiments showed relative standard deviation (R.S.D.) of peak area and retention time were better than 2.5%, inter-day and intra-day variabilities showed that R.S.D. was ranged from 0.66% to 4.17%. Accuracy validation showed that average recovery was between 95.33% and 104.10%. The method was validated to achieve the satisfactory precision and recovery. Relative retention time and relative peak area were used to identify the common peaks for fingerprint analysis. There are nine common peaks in the fingerprint. The quality of sixteen batches of Flos Lonicerae samples was evaluated by hierarchical clustering analysis, which classfied all bacthes into two clusters. Furthermore, the contents of important medicinal compounds (chlorogenic acid, macranthoidin B and dipsacoside B) in different batches of Flos Lonicerae samples were determined simultaneously using the developed HPLC-ELSD method. The developed analytical procedure was proved to be a reliable and rapid method for the quality control of Flos Lonicerae.

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