scholarly journals Selection of Rhizobium spp. Isolates for Inoculation of Field Pea (Pisum sativum)

2021 ◽  
Vol 25 (04) ◽  
pp. 845-850
Author(s):  
Çiğdem Küçük
Keyword(s):  
Nitrogen Fixation ◽  
Glutamine Synthetase ◽  
Dry Matter ◽  
Root Nodules ◽  
Bacterial Isolates ◽  
Streptomycin Sulphate ◽  
Microbiological Characteristics ◽  
Rhizobium Spp ◽  
Dry Matter Weight ◽  
Selection Of

Rhizobium isolates from wild pea nodules were characterized on the basis of microbiological characteristics. P4, P7, P12, P14, P16, P19, P20, P22, P23 and P24 isolates grew at the 4.5 pH, P5, P6, P11, P12, P13, P14, P16, P17, P19, P20 and P21 isolates grew at 4% NaCl and P7, P8, P10, P11, P12, P14, P19, P20, P22, P23, P24 and P25 isolates grew at 40°C. Resistance to antibiotics (μg mL-1) was investigated in a large propotion of isolates; streptomycin sulphate (80), rifampisin (40), erythromycin (30), chloramphenicol (100), Penicillin (40). In this study, local Rhizobium bacterial isolates were isolated from wild pea root nodules and their efficacy was investigated. Isolates significantly increased plant dry matter weight. The highest nitrogen fixation was achieved with P4 inoculation. Glutamine synthetase and leghemoglobin content of the nodules were determined in the inoculated with the highest P4 isolate. © 2021 Friends Science Publishers

scholarly journals ABSENCE OF A CORRELATION BETWEEN NITRATE REDUCTASE AND SYMBIOTIC NITROGEN FIXATION EFFICIENCY IN RHIZOBIUM MELILOTI

1980 ◽  
Vol 60 (1) ◽  
pp. 209-212 ◽  
Author(s):  
H. ANTOUN ◽  
L. M. BORDELEAU ◽  
D. PRÉVOST ◽  
R. A. LACHANCE
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Dry Matter ◽  
Symbiotic Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Specific Activities ◽  
Selection Of

Specific activities of the assimilatory and "regulatory" types of nitrate reductase were studied in 41 strains of Rhizobium meliloti having different symbiotic nitrogen fixation activities. Both nitrate reductase enzymes were present in very effective and ineffective strains and no significant correlation was found between the specific activities of the two enzymes and the dry matter yields of alfalfa obtained with the 41 strains. Measurements of the specific activities of the two nitrate reductase enzymes in the vegetative bacteria cannot be used as a rapid physiological test for the selection of very effective strains of R. meliloti.

Renodulation and nitrogen fixing potential of Acacia nilotica inoculated with Rhizobium isolates

1993 ◽  
Vol 39 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Banwari Lal ◽  
Sunil Khanna
Keyword(s):  
Nitrogen Fixation ◽  
Dry Matter ◽  
Nitrogenase Activity ◽  
Root Nodules ◽  
Acacia Nilotica ◽  
Resistance Pattern ◽  
Dry Matter Yield ◽  
Native Population ◽  
Rhizobium Isolate ◽  
Matter Production

Renodulation and nitrogen fixation potential of indigenous and exotic isolates of Rhizobium were studied in a field experiment with Acacia nilotica from July 1990 to June 1991. The inoculum isolates belonged to different serotypes and did not show cross-reaction with the native population of Rhizobium. Nitrogenase activity of root nodules observed at 4-month intervals covering three seasons snowed a decline during winter months, which corresponded with the senescence of the nodules. Maximal renodulation as checked by serotyping and antibiotic resistance pattern was observed with isolate AB 3 followed by AD 4 and USDA 3325. The highest nitrogenase activity was exhibited in plants inoculated with AD 4 at 12 months. Plants inoculated with Rhizobium isolate USDA 3325 showed the highest increase in dry-matter yield at 12 months. Except for AB 3, dual inoculation with Rhizobium isolates and Glomus fasciculatum did not enhance dry matter production as compared with uninoculated controls or single inoculation of either Rhizobium isolate or G. fasciculatum.Key words: Rhizobium, Acacia nilotica, nitrogenase activity, renodulation.

scholarly journals Expression Map for Genes Involved in Nitrogen and Carbon Metabolism in Alfalfa Root Nodules

1999 ◽  
Vol 12 (6) ◽  
pp. 526-535 ◽  
Author(s):  
Gian B. Trepp ◽  
Stephen J. Temple ◽  
Bruna Bucciarelli ◽  
Li Fang Shi ◽  
Carroll P. Vance
Keyword(s):  
Nitrogen Fixation ◽  
Glutamine Synthetase ◽  
Carbon Metabolism ◽  
Root Nodule ◽  
Root Nodules ◽  
Constitutive Expression ◽  
Nodule Development ◽  
Relative Distribution ◽  
Nitrogen Fixing ◽  
Alfalfa Root

During root nodule development several key genes involved in nitrogen fixation and assimilation exhibit enhanced levels of expression. However, little is known about the temporal and spatial distribution patterns of these transcripts. In a systematic study the transcripts for 13 of the essential enzymes involved in alfalfa (Medicago sativa) root nodule nitrogen and carbon metabolism were localized by in situ hybridization. A serial section approach allowed the construction of a map that reflects the relative distribution of these transcripts. In 33-day-old root nodules, the expression of nifH, NADH-dependent glutamate synthase (NADH-GOGAT; EC 1.4.1.14) and a cytosolic isoform of glutamine synthetase (GS13; GS; EC 6.3.1.2) were localized predominantly in a 5- to 15-cell-wide region in the distal part of the nitrogen-fixing zone. This zone was also the region of high expression for leghemoglobin, a second cytosolic glutamine synthetase isoform (GS100), aspartate aminotransferase-2 (AAT-2; EC 2.6.1.1), asparagine synthetase (AS; 6.3.5.4), phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31), and sucrose synthase (SuSy; EC 2.4.1.13). This suggests that, in 33-day-old alfalfa root nodules, nitrogen fixation is restricted to this 5- to 15-cell-wide area. The continued significant expression of the GS100 subclass of GS and AS in the proximal part of the nitrogen-fixing zone implicates these gene products in nitrogen remobilization. A low constitutive expression of NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) was observed throughout the nodule. The transcript distribution map will be used as a navigational tool to assist in developing strategies for the genetic engineering of alfalfa root nodules for enhanced nitrogen assimilation.

Phylogenetic and symbiotic characterization of rhizobial bacteria nodulating Argyrolobium uniflorum in Tunisian arid soils

2008 ◽  
Vol 54 (3) ◽  
pp. 209-217 ◽  
Author(s):  
M. Mahdhi ◽  
P. de Lajudie ◽  
M. Mars
Keyword(s):  
Nitrogen Fixation ◽  
16S Rrna ◽  
Root Nodules ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Arid Areas ◽  
Arid Soils ◽  
Bacterial Isolates ◽  
Phenotypic Features

Forty-two bacterial isolates from root nodules of Argyrolobium uniflorum growing in the arid areas of Tunisia were characterized by phenotypic features, RFLP, and sequencing of PCR-amplified 16S rRNA genes. The isolates were found to be phenotypically diverse. The majority of the isolates tolerated 3% NaCl and grew at temperatures up to 40 °C. Phylogenetically, the new isolates were grouped in the genera Sinorhizobium (27), Rhizobium (13), and Agrobacterium (2). Except for the 2 Agrobacterium isolates, all strains induced nodulation on Argyrolobium uniflorum, but the number of nodules and nitrogen fixation efficiency varied among them. Sinorhizobium sp. strains STM 4034, STM 4036, and STM 4039, forming the most effective symbiosis, are potential candidates for inoculants in revegetalisation programs.

Agricultural practices of improve hardiness a winter-annual rape

2016 ◽  
Vol 2 (91) ◽  
pp. 57-62
Author(s):  
O.L. Kyrylesko
Keyword(s):  
Dry Matter ◽  
Agricultural Practices ◽  
Mineral Fertilizers ◽  
Farming Practices ◽  
Winter Rape ◽  
Output Capacity ◽  
Careful Preparation ◽  
Digestible Protein ◽  
Winter Annual ◽  
Selection Of

Influence of top-dressing is considered in the article, norms and terms of sowing on of winter-annual rape. The assessment conducted by the yield of green mass and seeds, output capacity by about 1 hectare of dry matter, feed units and digestible protein, the number of dead plants and density of herbage. Established that hardiness and productivity of winter rape can be enhanced through the use of farming practices as: by creating a moderate density of herbage, using optimal terms of planting and doses of mineral fertilizers, selection of predecessors and careful preparation of the soil ect. The mechanism of influence of agrotechnical receptions is exposed on of winter-annual rape through determination in roots before the offensive of the winter of separate biochemical indexes (sugar, starch, to protein).

scholarly journals Potential impact of biocide adaptation on selection of antibiotic resistance in bacterial isolates

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Engy Elekhnawy ◽  
Fatma Sonbol ◽  
Ahmed Abdelaziz ◽  
Tarek Elbanna
Keyword(s):  
Antibiotic Resistance ◽  
Cross Resistance ◽  
Main Body ◽  
Bacterial Isolates ◽  
New Agents ◽  
Biocidal Products ◽  
Potential Impact ◽  
Emergence Of Resistance ◽  
Vital Factor ◽  
Selection Of

Abstract Background Antibiotic resistance in pathogenic bacterial isolates has increased worldwide leading to treatment failures. Main body Many concerns are being raised about the usage of biocidal products (including disinfectants, antiseptics, and preservatives) as a vital factor that contributes to the risk of development of antimicrobial resistance which has many environmental and economic impacts. Conclusion Consequently, it is important to recognize the different types of currently used biocides, their mechanisms of action, and their potential impact to develop cross-resistance and co-resistance to various antibiotics. The use of biocides in medical or industrial purposes should be monitored and regulated. In addition, new agents with biocidal activity should be investigated from new sources like phytochemicals in order to decrease the emergence of resistance among bacterial isolates.

scholarly journals Mutation in the ntrR Gene, a Member of the vap Gene Family, Increases the Symbiotic Efficiency of Sinorhizobium meliloti

2001 ◽  
Vol 14 (7) ◽  
pp. 887-894 ◽  
Author(s):  
Boglárka Oláh ◽  
Erno Kiss ◽  
Zoltán Györgypál ◽  
Judit Borzi ◽  
Gyöngyi Cinege ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Pathogenic Bacteria ◽  
Sinorhizobium Meliloti ◽  
Root Nodules ◽  
Nifh Gene ◽  
Dry Weight ◽  
Nodule Occupancy ◽  
Gene Encoding ◽  
Symbiotic Efficiency ◽  
Host Interactions

In specific plant organs, namely the root nodules of alfalfa, fixed nitrogen (ammonia) produced by the symbiotic partner Sinorhizobium meliloti supports the growth of the host plant in nitrogen-depleted environment. Here, we report that a derivative of S. meliloti carrying a mutation in the chromosomal ntrR gene induced nodules with enhanced nitrogen fixation capacity, resulting in an increased dry weight and nitrogen content of alfalfa. The efficient nitrogen fixation is a result of the higher expression level of the nifH gene, encoding one of the subunits of the nitrogenase enzyme, and nifA, the transcriptional regulator of the nif operon. The ntrR gene, controlled negatively by its own product and positively by the symbiotic regulator syrM, is expressed in the same zone of nodules as the nif genes. As a result of the nitrogen-tolerant phenotype of the strain, the beneficial effect of the mutation on efficiency is not abolished in the presence of the exogenous nitrogen source. The ntrR mutant is highly competitive in nodule occupancy compared with the wild-type strain. Sequence analysis of the mutant region revealed a new cluster of genes, termed the “ntrPR operon,” which is highly homologous to a group of vap-related genes of various pathogenic bacteria that are presumably implicated in bacterium-host interactions. On the basis of its favorable properties, the strain is a good candidate for future agricultural utilization.

scholarly journals NITROGEN FIXATION BY EXCISED SOY BEAN ROOT NODULES

1954 ◽  
Vol 208 (1) ◽  
pp. 29-39
Author(s):  
M.H. Aprison ◽  
Wayne E. Magee ◽  
R.H. Burris
Keyword(s):  
Nitrogen Fixation ◽  
Root Nodules ◽  
Bean Root

scholarly journals Growth and yield performance of soybean with the application of Bradyrhyzobium inoculant via furrow and seed

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2387
Author(s):  
Santiel Alves Vieira Neto ◽  
Fábio Ribeiro Pires ◽  
João Carlos Madalão ◽  
Douglas Gomes Viana ◽  
Carlos César Evangelista de Menezes ◽  
...  
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Agricultural Production ◽  
Aerial Part ◽  
Dry Matter ◽  
Growth And Yield ◽  
Recommended Dose ◽  
Yield Performance ◽  
Seed Inoculation ◽  
Dry Matter Weight

Given the high costs of agricultural production, especially due to the price of fertilisers, particularly nitrogen, the use of inoculants to supply nitrogen to soybean crops is a widely recommended practice. The objective of this study was to evaluate the feasibility of applying inoculants through seed and planting furrow in soil previously cultivated with soybean and Brazilian native “cerrado” biome soil under greenhouse conditions. Seven treatments were tested: 1) inoculation via seed (inoculant + fungicide + micronutrient), 2) treatment via seed (fungicide + micronutrient), 3) control (only seed), 4) inoculation via furrow-dose 1 (recommended dose), 5) inoculation via furrow-dose 2 (twice the recommended dose), 6) inoculation via furrow-dose 3 (three times the recommended dose) and 7) inoculation via furrow-dose 1 + seed inoculation. We evaluated plant height, fresh and dry matter weight of the aerial part and nodules, number of total, viable and non-viable nodules, number of pods per plant and grain yield. Inoculation was more effective when used in cerrado soil, but soybean performance in treatments without inoculation was higher in previously cultivated soil. Application through furrow proved to be a viable practice due to the similarity of the results obtained with the traditional application by seed.

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