scholarly journals Production of Antifungal Gelatinase by Enterococcus durans S2C and Its Potential Role in the Biological Control

2021 ◽  
Vol 25 (05) ◽  
pp. 1043-1050
Author(s):  
Zafar Hayat
Keyword(s):  
Antifungal Activity ◽  
Food Industry ◽  
Potential Role ◽  
Economic Value ◽  
Pathogenic Fungi ◽  
Enterococcus Durans ◽  
Normal Microbiota ◽  
Raw Cow’S Milk ◽  
Culture Dependent ◽  
Low Rate

Probiotic bacteria are good sources for antimicrobial and are normal microbiota of the gastrointestinal tract of animals and humans. Enterococci are found everywhere in nature and have been utilized as probiotics in the food industry. In the present study, Enterococcus durans S2C strain was isolated from raw cow’s milk using the culture-dependent method and evaluated for antifungal properties. The strain S2C showed promising extracellular proteolytic activity and the extracellular peptide was an important source of antifungal activity. Besides, a low rate of antibiotic resistance and non-hemolytic activity was detected in E. durans S2C. Strong antifungal activity of E. durans against two plant pathogenic fungi namely Fusarium oxysporum and Rhizoctonia solani was also recorded. Antifungal activity evaluation showed that R. solani colony was the most inhibited fungus followed by F. oxysporum. Gelatinase enzyme having antifungal activity was also purified from E. durans S2C isolate. This study concludes that the environmental-derived E. durans S2C strain can be selected for technological application to control pathogenic fungi and to protect the economic value of the crops. © 2021 Friends Science Publishers

Homogenate Extraction of Dihydroartemisinin from Artemisia Hedinii and Its Antifungal Activity

2021 ◽  
Author(s):  
Cong You ◽  
Jun Yu ◽  
Guangjiong Qin ◽  
JinPeng Yang ◽  
Chunlei Yang ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Crude Extract ◽  
Spore Germination ◽  
Alternaria Alternata ◽  
Low Cost ◽  
Pathogenic Fungi ◽  
Extraction Yield ◽  
Optimal Conditions ◽  
Extraction Technology ◽  
Homogenate Extraction

Abstract Background Artemisia hedinii is a well-known traditional Chinese medicine. It can be used to extract dihydroartemisinin (DHA). Objective The purpose of this study was to explore the optimal conditions for the homogenate extraction of DHA from A. hedinii and the antifungal activity of DHA. Methods In this study, single factor experiments and response surface method were used to determine the optimal extraction conditions of crude extract and DHA, the method of spore germination was used to study the antifungal activity of DHA to Alternaria alternata. Result The optimal conditions were found as fellow: ratio of liquid to material 22 mL/g; Extraction time 60 s; soaking time 34 min. Under these conditions, extraction yield of DHA was (1.76 ± 0.04%). When the concentration of crude extract were 0.5 and 8 mg/mL, the spore germination inhibition rates of Alternaria alternata were (17.00 ± 2.05%) and (92.56 ± 2.01%), which were 3.34 and 1.15 times that of DHA standard, respectively. Conclusion Homogenate extraction technology is a fast and efficient method to extract DHA from A. hedinii. The crude extract has significant antifungal activity against A. alternata with low cost, which provides a possibility for the use of DHA in the prevention and treatment of plant pathogenic fungi. Highlights The optimum conditions of the extraction of DHA from A. hedinii by homogenate extraction were obtained. DHA has antifungal activity against A. alternata. Compared with pure DHA, the crude extract has stronger antifungal activity against A. alternata.

scholarly journals Chemical Composition and Antimicrobial Properties of Mentha × piperita cv. ‘Kristinka’ Essential Oil

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1567
Author(s):  
Ippolito Camele ◽  
Daniela Gruľová ◽  
Hazem S. Elshafie
Keyword(s):  
Antifungal Activity ◽  
Xanthomonas Campestris ◽  
Fruits And Vegetables ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Cell Membrane Permeability ◽  
Penicillium Expansum ◽  
Mentha Piperita ◽  
Plant Essential Oils ◽  
Main Components

Several economically important crops, fruits and vegetables are susceptible to infection by pathogenic fungi and/or bacteria postharvest or in field. Recently, plant essential oils (EOs) extracted from different medicinal and officinal plants have had promising antimicrobial effects against phytopathogens. In the present study, the potential microbicide activity of Mentha × piperita cv. ‘Kristinka’ (peppermint) EO and its main constituents have been evaluated against some common phytopathogens. In addition, the cell membrane permeability of the tested fungi and the minimum fungicidal concentrations were measured. The antifungal activity was tested against the following postharvest fungi: Botrytis cinerea, Monilinia fructicola, Penicillium expansum and Aspergillus niger, whereas antibacterial activity was evaluated against Clavibacter michiganensis, Xanthomonas campestris, Pseudomonas savastanoi and P. syringae pv. phaseolicola. The chemical analysis has been carried out using GC-MS and the main components were identified as menthol (70.08%) and menthone (14.49%) followed by limonene (4.32%), menthyl acetate (3.76%) and β-caryophyllene (2.96%). The results show that the tested EO has promising antifungal activity against all tested fungi, whereas they demonstrated only a moderate antibacterial effect against some of the tested bacteria.

Antifungal activity of phthalide lactones on plant pathogenic fungi and yeast

2018 ◽  
Vol 280 ◽  
pp. S27
Author(s):  
Enriqueta Martinez Rojas ◽  
Fatuma Hassan ◽  
Filip Boratynski ◽  
Teresa Olejniczak ◽  
Karl Steffens ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi

Design, Synthesis and Evaluate In Vitro Antifungal Activity of Novel Benzamide Derivatives

2019 ◽  
Vol 41 (3) ◽  
pp. 549-549
Author(s):  
Xuesong Wang and Xiaorong Tang Xuesong Wang and Xiaorong Tang
Keyword(s):  
Antifungal Activity ◽  
Pathogenic Fungi ◽  
Gibberella Zeae ◽  
Design Synthesis ◽  
Carboxylic Acid Amides ◽  
Almost All ◽  
Negative Controls ◽  
New Compounds ◽  
Benzamide Derivatives

A series of novel benzamide derivatives according to fluopicolide were designed and synthesized following the rule of combination carboxylic acid amides and amines derivatives together. The antifungal activity of the 15 new compounds were evaluated in vitro against five pathogenic fungi, including Sclerotinia sclerotiorum, Gibberella zeae, Rhizoctonia solani, Helminthosporium maydis and Botrytis cinerea. Almost all the structure have not been reported, except compounds 3, 5 and 6. A surprising finding is that all the five tested fungi breed faster than negative controls when supplementary with compound 715 , respectively.

scholarly journals Antifungal Activity of Euclea divinorum Root and Study of its Ethnobotany and Phytopharmacology

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 680 ◽  
Author(s):  
Al-Fatimi
Keyword(s):  
Antifungal Activity ◽  
Plant Root ◽  
Pathogenic Fungi ◽  
Microsporum Gypseum ◽  
Root Bark ◽  
Ethnobotanical Survey ◽  
Traditional Use ◽  
Absidia Corymbifera ◽  
Methanolic Extracts ◽  
Antibiotic Drug

The ethnobotanical survey of Euclea divinorum Hiern (Ebenaceae) was conducted on Soqotra Island, Yemen. The root bark is used to treat mouth, dental, dermal and blood diseases in the traditional medicine of the island. The study is the first report about the effect of the plant root barks against six human pathogenic fungi. The non-polar dichloromethane extract of Euclea divinorum root bark showed stronger antifungal activities compared to polar direct and sequential methanolic extracts. These extracts showed significant broad antifungal activity against Absidia corymbifera, Aspergillus fumigatus, Candida krusei, Microsporum gypseum, Mucor sp. and Trichophyton mentagrophytes compared to the standard antibiotic drug nystatin. Thin-layer chromatography (TLC) revealed the presence of the naphthoquinones in the extracts. The results showed an extraction process to separate most antifungal naphthoquinones from the root bark by using non-polar solvent dichloromethane, while flavonoids remained in the polar methanolic extracts; therefore, the polar sequential and direct methanolic extracts recorded higher antioxidant activity than the non-polar extracts with less toxicity. The biological and chemical results identified the presence of antifungal and antioxidant constituents in the root bark and confirm its traditional use in Soqotra Island as crude powder to treat dental and dermal diseases and to clean teeth. Moreover, our results are compared with literature review on ethnobotany and phytopharmacology of the E. divinorum to present a medicinal monograph about the plant.

In vitrokinetics and antifungal activity of various extracts ofTerminalia chebulaseeds against plant pathogenic fungi

2010 ◽  
Vol 43 (8) ◽  
pp. 801-809 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Atiqur Rahman ◽  
Shruti Shukla ◽  
Savita Shukla ◽  
S.M. Yassir Arafat ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi

scholarly journals ANTI-CANDIDA ALBICANS ACTIVITY OF THE ASSOCIATION OF CITRONELAL WITH ANFOTERICIN B OR WITH CETOCONAZOLE

2019 ◽  
Vol 16 (31) ◽  
pp. 250-257
Author(s):  
Patrícia Duarte Costa SILVA ◽  
Brenda Lavínia Calixto dos SANTOS ◽  
Gustavo Lima SOARES ◽  
Wylly Araújo de OLIVEIRA
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Amphotericin B ◽  
Inhibitory Concentration ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Mortality Rates ◽  
New Drugs ◽  
High Morbidity ◽  
Isolated Species

Fungal infections caused by species of the genus Candida are responsible for high morbidity and mortality rates, mainly affecting immunocompromised individuals. Among fungi, Candida albicans is the most frequently isolated species of clinical specimens. A problem associated with increased resistance of pathogenic fungi to the agents used in the therapeutic regimen and the limited number of drugs to cure these infections. As a result, the search for new drugs with antifungal activity has become increasingly important. The aim of this study is to study the antifungal activity of citronellal alone and in combination with amphotericin B or ketoconazole. The Minimal Inhibitory Concentration of citronellal, amphotericin B and ketoconazole against strains of Candida albicans were evaluated by the microdilution technique, and the Minimum Fungicide Concentration of citronellal against the same strains was also performed. Through the checkerboard methodology the effect of the combination of citronelal with amphotericin B or with ketoconazole was determined. This study showed that the association of citronellal with ketoconazole was shown to be an additive against one of the strains of C. albicans and indifferent to another strain. While the combined activity of citronellal and amphotericin B demonstrated an indifferent effect on the strains tested.

scholarly journals PERBANDINGAN KEMAMPUAN EKSTRAK KULIT PISANG AGUNG SEMERU DAN PISANG MAS KIRANA VARIETAS LUMAJANG DALAM MENGHAMBAT PERTUMBUHAN Candida albicans

2017 ◽  
Vol 4 (2) ◽  
pp. 31
Author(s):  
Dwi Nur Rikhmasari
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Pathogenic Fungi ◽  
Optimal Concentration ◽  
Banana Peel ◽  
Anova Test ◽  
Random Design ◽  
The Difference ◽  
Peel Extract ◽  
Better Than

<p align="center"> </p><p><em>A</em><em>gung Semeru </em><em>banana </em><em>and </em><em>M</em><em>as Kirana, is one of the typical banana varieties found in Lumajang Regency. Where on the </em><em>banana peel extraction</em><em> contain various antifungi compounds. The purpose of this research is to know the difference of banana extract of Agung Semeru and banana Mas Kirana varieties of lumajang, in inhibiting the growth of pathogenic fungi Candida albicans, and to know the optimal concentration which can inhibit the growth of Candida albicans. The method used was Completely Random Design (RAL) with four of factors, that is: 0, 25, 50, 75 and 100 %. The results were analyzed with Anova test and Duncan test multiple ranger test at the level of 5%. Each treatment was repeated three times. </em><em>The results show that there is an effect of giving extract of Semeru banana peel (sig = 0,00) and banana Mas Kirana (sig = 0,00) to Candida albicans growth at various concentration (0%, 25%, 50%, 75% and 100%). For extract of Semeru banana peel at concentration 75% (</em><em>3,19  ± 0,84</em><em> mm) showed better result from other concentration, while for banana Mas Kirana Lumajang peel extract, at concentration 50% (</em><em>2,44 ± 0,0 mm</em><em>) showed better result than treatment other. Ability of antifungal activity of Agung Semeru banana peel extract better than banana Mas Kirana.</em></p><p><em> </em></p>

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