scholarly journals Haemato-biochemical profile and milk leukocyte count in subclinical and clinical mastitis affected crossbred cattle

2017 ◽  
Vol 5 (1) ◽  
pp. 001-006
Author(s):  
Sarvesha K ◽  
◽  
Satyanarayana M L ◽  
Narayanaswamy H D ◽  
Rao S ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Clinical Mastitis ◽  
Biochemical Profile ◽  
Crossbred Cattle

Direct blood PCR detection of Babesia bigemina and its effect on haematological and biochemical profile in crossbred cattle of eastern Haryana

2017 ◽  
Author(s):  
Anita Ganguly ◽  
R. S. Bisla ◽  
Indrajit Ganguly ◽  
Harpreet Singh ◽  
Vandna Bhanot ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Pcr Detection ◽  
Biochemical Profile ◽  
Crossbred Cattle ◽  
Babesia Bigemina ◽  
Direct Pcr ◽  
Blood Samples ◽  
Crossbred Cows ◽  
Specificity And Sensitivity ◽  
Healthy Control

The present study aimed to diagnose Babesia bigemina in naturally infected crossbred cows and to determine its effect on haemato-biochemical profile of host animals. Blood samples from lactating crossbred cows (n=30) between 3-6 years of age and showing clinical signs of babesiosis were collected, with or without anticoagulant, and analyzed for the protozoa by direct smear, direct blood PCR detection of the apical membrane antigen 1 (AMA-1) gene specific amplicon of B. bigemina and estimation of haematological and biochemical parameters. Healthy crossbred cows (n=10), examined free from haemoprotozoan infections were included as control. Blood Direct PCR revealed a 448-bp amplified fragment. Out of 150 random blood samples screened, (27/150) 18% were positive under light microscope, whereas direct blood PCR revealed (39/150) 26% samples positive for B. bigemina. The result shows higher specificity and sensitivity of PCR test over blood smear examination. The infected group showed significantly (p less than 0.001) decreased levels of TEC (3.04±0.19), Hb (4.78±0.27) and PCV (14.53 ±0.87) than healthy control animals. However, differences in the red blood cell indices (MCV, MCH and MCHC) were non-significant (p>0.05) between the groups indicating normocytic hypochromic anaemia in affected crossbred cattle. Serum samples of infected cows showed significantly (p less than 0.01) higher values of ALT (78.83±8.95), AST (146.13±7.62), BUN (27.09±1.02), creatinine (1.93±0.1) and TBIL (1.42±0.06) than that of healthy control. A significant decrease (p less than 0.01) of TSP (6.12±0.13) and albumin (2.39±0.09) was also recorded in the infected cows compare to healthy control. The standardized blood direct PCR method of the present investigation may be useful for rapid and reliable diagnosis of B. bigemina in conjunction with microscopic examination. Moreover, marked changes in haematological and serum biochemical profile observed in B. bigemina infected crossbred cows may be useful in understanding disease pathogenesis and undertaking necessary corrective measures..

Immunological profiles of lactating and mastitis crossbred cattle

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
Kuldeep Rai ◽  
Manju Ashutosh ◽  
Sakainder Singh ◽  
Ashok Baindha ◽  
Virendra Kumar ◽  
...  
Keyword(s):  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Crossbred Cattle ◽  
Immune Markers ◽  
Lactating Cows ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

In order to assess the immune profiles of lactating and mastitis crossbred cows, blood samples were collected weekly for one month from early, mid and late lactating cows and once from clinical and subclinical mastitis cows. Immune markers nitric oxide, tumor necrosis factor α and immunoglobulin G were estimated in blood plasma. A concurrent significant (P<0.05) fall in IgG concentration was noted in clinical mastitis cows and was highest during mid lactation in lactating cows. The clinical and subclinical mastitis cows had significantly (P<0.05) high levels of TNF-α and were non-detectable in lactating cows. Plasma NO level exhibited a massive rise (P<0.05) in mastitis cows (clinical and subclinical), the levels were significantly (P<0.05) higher than in the normal lactating states.

Lactoferrin gene promoter variants and their association with clinical and subclinical mastitis in indigenous and crossbred cattle

2015 ◽  
Vol 18 (3) ◽  
pp. 465-471 ◽  
Author(s):  
A. Chopra ◽  
I.D. Gupta ◽  
A. Verma ◽  
A.K. Chakravarty ◽  
V. Vohra
Keyword(s):  
Regulatory Region ◽  
Gene Promoter ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
North India ◽  
Bovine Lactoferrin ◽  
Crossbred Cattle ◽  
Single Nucleotide ◽  
Lactoferrin Gene ◽  
Karan Fries

Abstract Lactoferrin (Lf) gene promoter was screened for the presence of single nucleotide polymphism in indigenous and crossbred cattle from North India and to evaluate its association with Mastitis. Study revealed the presence of genetic variation in regulatory region of bovine Lactoferrin gene using PCR-RFLP technique. Three genotypes namely GG, GH and HH were identified. A single nucleotide change, from guanine to adenine at 25th position was found to be significantly associated (p<0.05) with clinical mastitis in indigenous Sahiwal and crossbred Karan Fries cattle maintained at organised herd of National Dairy Research Institute, Karnal. A non-significant association was observed between subclinical mastitis, somatic cell score (SCS), and GG genotype in Karan Fries cattle, however, a lower SCS was observed in animals having GG genotype. Overall a lower incidence of clinical mastitis was recorded in those animals having GG genotype of Lf in Sahiwal and Karan Fries (KF) cattle. The SNP identified in the promoter region may effect expression lactoferrin protein, which may lead to different levels of antibacterial and anti-inflammatory activity of Lf gene. Results from this study indicated the probable role played by Lactoferrin promoter to serve as candidate gene for mastitis susceptibility among indigenous and crossbred milch cattle.

Allelic diversity at BoLA DRB3 locus and association with predisposition to clinical mastitis in indicus and crossbred cattle

2021 ◽  
pp. 1-10
Author(s):  
Shubham Loat ◽  
Namita Kumari ◽  
Shallu Saini ◽  
M. S. Dige ◽  
Anurag Kumar ◽  
...  
Keyword(s):  
Allelic Diversity ◽  
Clinical Mastitis ◽  
Crossbred Cattle

Phenotypic classification and biochemical profile of obesity for cardiovascular prevention

2020 ◽  
Vol 179 (5) ◽  
Author(s):  
Marco A. Perrone ◽  
Jagadish Babu Dasari ◽  
Alfredo Intorcia ◽  
Paola Gualtieri ◽  
Massimo Marche ◽  
...  
Keyword(s):  
Cardiovascular Prevention ◽  
Biochemical Profile ◽  
Phenotypic Classification

Crossbred Cattle Adoption and its Implications on Raising Employment in the Smallholder Dairying of Rural Assam

2020 ◽  
pp. 81-88
Keyword(s):  
Development Programme ◽  
Farm Households ◽  
Crossbred Cattle ◽  
Dairy Farmers ◽  
Cross Sectional ◽  
Extension Services ◽  
Cooperative Society ◽  
Average Size ◽  
Smallholder Dairying ◽  
Dairy Development

ABSTRACT Using cross-sectional data of 245 smallholder rural dairy farmers and propensity score matching, the present study attempted to estimate the treatment effect of changing the breed of cattle (indigenous to crossbred) on changes in labour use in Assam. The analysis indicated that crossbred cattle adopters had higher herd sizes, access to extension services, membership of dairy cooperative society (DCS) and the majority of them are beneficiary of government dairy development programme. The estimation results show that crossbred cattle adopters had significantly higher labour use for selected dairy farming operations. This points out that there was a higher employment prospect in the adoption of crossbred cattle. Additionally, the study pointed out that crossbred cattle adoption was associated with higher employment effect on small to average size farms and farm households with literate farmers. The findings of the study recommend that there is a need for adequate diffusion of breeding technology such as artificial insemination (AI) or deployment of pure-breed exotic bulls in not so easily accessible areas to facilitate the small and medium farmers with crossbred cattle adoption for generating employment along with augmenting productivity and income.

STUDI GAMBARAN HASIL PEMERIKSAAN APUSAN DARAH TEPI PADA IBU HAMIL DI LABORATORIUM RSUD H.A.SULTHAN DAENG RADJA KABUPATEN BULUKUMBA

2018 ◽  
Vol 3 (2) ◽  
pp. 13-26
Author(s):  
Rahmat Aryandi ◽  
Subakhir Salnus
Keyword(s):  
Platelet Count ◽  
Pregnant Women ◽  
Blood Smear ◽  
Leukocyte Count ◽  
Marked Change ◽  
Peripheral Blood Smear ◽  
Morphological Examination ◽  
Laboratory Observation ◽  
Hematologic Disorder ◽  
Normal Platelet

During pregnancy, there will be a marked change in anatomy, physiology and biochemistry since the onset of pregnancy and often lackof nutrient intake. Hematologic disorder is often found in pregnant women because it causes pregnant women more susceptible to disturbances in blood circulation, The purpose of this study to determine the description of blood smear results in pregnant women in the laboratory RSUD H.A.Sulthan Daeng Radja District. This research is descriptive with laboratory observation approach. The sample used in this study were 30 samples of pregnant women who checked themselves in the Laboratory of RSUDH.A.Sulthan Daeng Radka Bulukumba District. The result of this research showed the result of peripheral blood smear on the morphology of erythrocytes using 30 samples of pregnant women showed 14 samples (46,66%) normocytic normochrom and the remaining 16 samples were morphological variation (53,33%), on morphological examination and platelet count with using 30 samples of pregnant women showed each 29 samples had morphology and normal platelet counts with respectively 96.66% percentage and platelet aggregation and decreased platelet count (thrombocytopenia) with each persentase 3.33%. At leukocyte morphology examination using 30 samples of pregnant women showed 29 samples had normal morphology with 96,66% percentage and one sample with hypersegmentation with percentage 3,33%. normal leukocyte count at 9 samples with percentage 30% and leukocyte count increased at 21 samples with percentage 70%.

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