Lactoferrin gene promoter variants and their association with clinical and subclinical mastitis in indigenous and crossbred cattle

Abstract Lactoferrin (Lf) gene promoter was screened for the presence of single nucleotide polymphism in indigenous and crossbred cattle from North India and to evaluate its association with Mastitis. Study revealed the presence of genetic variation in regulatory region of bovine Lactoferrin gene using PCR-RFLP technique. Three genotypes namely GG, GH and HH were identified. A single nucleotide change, from guanine to adenine at 25th position was found to be significantly associated (p<0.05) with clinical mastitis in indigenous Sahiwal and crossbred Karan Fries cattle maintained at organised herd of National Dairy Research Institute, Karnal. A non-significant association was observed between subclinical mastitis, somatic cell score (SCS), and GG genotype in Karan Fries cattle, however, a lower SCS was observed in animals having GG genotype. Overall a lower incidence of clinical mastitis was recorded in those animals having GG genotype of Lf in Sahiwal and Karan Fries (KF) cattle. The SNP identified in the promoter region may effect expression lactoferrin protein, which may lead to different levels of antibacterial and anti-inflammatory activity of Lf gene. Results from this study indicated the probable role played by Lactoferrin promoter to serve as candidate gene for mastitis susceptibility among indigenous and crossbred milch cattle.

Download Full-text

Immunological profiles of lactating and mastitis crossbred cattle

Indian Journal of Animal Research ◽

10.18805/ijar.7047 ◽

2015 ◽

Vol 49 (6) ◽

Author(s):

Kuldeep Rai ◽

Manju Ashutosh ◽

Sakainder Singh ◽

Ashok Baindha ◽

Virendra Kumar ◽

...

Keyword(s):

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Subclinical Mastitis ◽

Clinical Mastitis ◽

Crossbred Cattle ◽

Immune Markers ◽

Lactating Cows ◽

Tnf Α ◽

Factor Α ◽

Necrosis Factor

In order to assess the immune profiles of lactating and mastitis crossbred cows, blood samples were collected weekly for one month from early, mid and late lactating cows and once from clinical and subclinical mastitis cows. Immune markers nitric oxide, tumor necrosis factor α and immunoglobulin G were estimated in blood plasma. A concurrent significant (P<0.05) fall in IgG concentration was noted in clinical mastitis cows and was highest during mid lactation in lactating cows. The clinical and subclinical mastitis cows had significantly (P<0.05) high levels of TNF-α and were non-detectable in lactating cows. Plasma NO level exhibited a massive rise (P<0.05) in mastitis cows (clinical and subclinical), the levels were significantly (P<0.05) higher than in the normal lactating states.

Download Full-text

Quarter-wise prevalence of subclinical mastitis in crossbred cows

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.7604 ◽

2017 ◽

Author(s):

Rachna Ullah ◽

Manju Ashutosh ◽

Sujata Pandita ◽

Ashutosh . ◽

Panjab Singh Yadav ◽

...

Keyword(s):

Subclinical Mastitis ◽

Clinical Mastitis ◽

Research Needs ◽

Crossbred Cows ◽

Lactating Cows ◽

California Mastitis Test ◽

Karan Fries

The present study was designed to determine the quarter-wise and animal-wise prevalence of sub-clinical mastitis in crossbred Karan Fries (Holstein x Tharparker) lactating cows. For this purpose, the udders of 73 cows were screened by modified California Mastitis Test (mCMT) and EC measurements. The efficiency of detection of mastitis and non-mastitis by EC measurements was 86% and 93% respectively. Animal-wise prevalence was found 41%, while quarter-wise prevalence was 31.16%. Among mastitic quarters, prevalence of single quarter infection, two quarter infection, three quarter infection and all four quarter infection per animal, was 0.00, 26.66, 43.33 and 30.00%, respectively. Out of all quarters, maximum prevalence was found in right hind quarters (29.67%) followed by left hind (26.37%), left fore (23.07%) and right fore (20.87%).The same pattern was observed during EC measurements. Though EC showed similarity with CMT in the detection of subclinical mastitis, the research needs to be carried out on more number of animals.

Download Full-text

Detection and Characterization of PCR-SSCP Markers of the Bovine Lactoferrin Gene for Clinical Mastitis

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.2006.1399 ◽

2006 ◽

Vol 19 (10) ◽

pp. 1399-1403 ◽

Cited By ~ 2

Author(s):

Zhou Lei ◽

Yuan-Yuan Yang ◽

Zhong-Hao Li ◽

Li-Juan Kong ◽

Guan-Dong Xing ◽

...

Keyword(s):

Clinical Mastitis ◽

Bovine Lactoferrin ◽

Lactoferrin Gene

Download Full-text

Characterisation of single nucleotide polymorphisms identified in the bovine lactoferrin gene sequences across a range of dairy cow breeds

Biochimie ◽

10.1016/j.biochi.2008.05.011 ◽

2009 ◽

Vol 91 (1) ◽

pp. 68-75 ◽

Cited By ~ 16

Author(s):

F OHALLORAN ◽

B BAHAR ◽

F BUCKLEY ◽

O OSULLIVAN ◽

T SWEENEY ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Dairy Cow ◽

Bovine Lactoferrin ◽

Nucleotide Polymorphisms ◽

Gene Sequences ◽

Single Nucleotide ◽

Lactoferrin Gene

Download Full-text

Fas gene promoter –670 polymorphism in gynecological cancer

International Journal of Gynecological Cancer ◽

10.1136/ijgc-00009577-200602001-00028 ◽

2006 ◽

Vol 16 (Suppl 1) ◽

pp. 179-182 ◽

Cited By ~ 2

Author(s):

M. Ueda ◽

Y. Terai ◽

K. Kanda ◽

M. Kanemura ◽

M. Takehara ◽

...

Keyword(s):

Ovarian Cancer ◽

Cervical Cancer ◽

Cancer Patients ◽

Germ Line ◽

Gynecological Cancer ◽

Gene Promoter ◽

Single Nucleotide ◽

Increased Risk ◽

Significant Difference ◽

Germ Line Polymorphism

Single-nucleotide polymorphism at −670 of Fas gene promoter (A/G) was examined in a total of 354 blood samples from normal healthy women and gynecological cancer patients. They consisted of 95 normal, 83 cervical, 108 endometrial, and 68 ovarian cancer cases. Eighty-three patients with cervical cancer had statistically higher frequency of GG genotype and G allele than 95 controls (P= 0.0353 and 0.0278, respectively). There was no significant difference in the genotype or allele prevalence between control subjects and endometrial or ovarian cancer patients. The Fas −670 GG genotype was associated with an increased risk for the development of cervical cancer (OR = 2.56, 95% CI = 1.08–6.10) compared with the AA genotype. The G allele also increased the risk of cervical cancer (OR = 1.60, 95% CI = 1.05–2.43) compared with the A allele. Germ-line polymorphism of Fas gene promoter −670 may be associated with the risk of cervical cancer in a Japanese population.

Download Full-text

Characterization of Escherichia coli Strains Derived from Cow Milk of Subclinical and Clinical Cases of Mastitis

Applied Sciences ◽

10.3390/app11020541 ◽

2021 ◽

Vol 11 (2) ◽

pp. 541

Author(s):

Katarzyna Grudlewska-Buda ◽

Krzysztof Skowron ◽

Ewa Wałecka-Zacharska ◽

Natalia Wiktorczyk-Kapischke ◽

Jarosław Bystroń ◽

...

Keyword(s):

Escherichia Coli ◽

Bacterial Species ◽

Subclinical Mastitis ◽

Clinical Mastitis ◽

Economic Problem ◽

Cow Milk ◽

P Value ◽

Dairy Herds ◽

E Coli ◽

Bonferroni Test

Mastitis is a major economic problem in dairy herds, as it might decrease fertility, and negatively affect milk quality and milk yield. Out of over 150 bacterial species responsible for the udder inflammation, Escherichia coli is one of the most notable. This study aimed to assess antimicrobial susceptibility, resistance to dipping agents and biofilm formation of 150 E. coli strains isolated from milk of cows with subclinical and clinical mastitis. The strains came from three dairy herds located in Northern and Central Poland. The statistical analyses were performed with post-hoc Bonferroni test and chi-square test (including Yates correction). The data with a p value of <0.05 were considered significant. We found that the tested strains were mostly sensitive to antimicrobials and dipping agents. It was shown that 37.33% and 4.67% of strains were resistant and moderately resistant to at least one antimicrobial agent, respectively. No extended-spectrum beta-lactamases (ESBL)-producing E. coli were detected. The majority of strains did not possess the ability to form biofilm or formed a weak biofilm. The strong biofilm formers were found only among strains derived from cows with subclinical mastitis. The lowest bacteria number was noted for subclinical mastitis cows’ strains, after stabilization with iodine (3.77 log CFU × cm−2) and chlorhexidine (3.96 log CFU × cm−2) treatment. In the present study, no statistically significant differences in susceptibility to antibiotics and the ability to form biofilm were found among the strains isolated from cows with subclinical and clinical mastitis. Despite this, infections in dairy herds should be monitored. Limiting the spread of bacteria and characterizing the most common etiological factors would allow proper treatment.

Download Full-text